BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmmt27c19
(688 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC27F1.07 |||dolichyl-diphospho-oligosaccharide-protein glycos... 61 2e-10
SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyce... 30 0.36
SPBC12C2.05c |||diacylglycerol binding protein Bzz1 |Schizosacch... 29 0.83
SPAC22H10.10 |alp21|sto1|tubulin specific chaperone cofactor E|S... 27 3.4
SPBC3E7.01 |fab1|ste12, SPBC6B1.11c|1-phosphatidylinositol-3-pho... 26 5.9
SPCC550.04c |gpi2||pig-C|Schizosaccharomyces pombe|chr 3|||Manual 26 5.9
SPAC227.13c |isu1||mitochondrial iron-sulfur cluster assembly sc... 26 5.9
SPAC6F6.06c |rax2||cell polarity factor Rax2|Schizosaccharomyces... 26 5.9
SPAC17C9.07 |alg8||glucosyltransferase Alg8|Schizosaccharomyces ... 25 7.8
>SPAC27F1.07 |||dolichyl-diphospho-oligosaccharide-protein
glycosyltransferase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 450
Score = 60.9 bits (141), Expect = 2e-10
Identities = 32/148 (21%), Positives = 71/148 (47%), Gaps = 2/148 (1%)
Frame = +1
Query: 181 NVDRTVDISTQLVKVVSKIVLENSGKSSVKHFLFAVEEAAKNNLAFIGA--KDSNSKDLR 354
N RT+D+ + K ++ +++ N G + + + + ++ + K+ K
Sbjct: 26 NAIRTIDLRSTYTKTLTSLIIVNEGDEPQNTYRYFQQTSPGESVLSLSVSIKEEEKKGTN 85
Query: 355 LAETTVKGYDYVKFWRVELKEPLNAGATVNVVVETVFTKALQPYPTAITQQEDQFVKYVG 534
+ YD + L+ P+ G + ++++ ++P P A+ Q E+Q++ Y+
Sbjct: 86 PIKVNNNVYD------IPLQPPVKPGESRTLLIQAGLDGGVRPLPAAVDQDEEQYLVYLT 139
Query: 535 NLYTYSPYYTSTQKTSVILNTKTVESFT 618
LY SPY T Q+T +IL + ++++T
Sbjct: 140 TLYLDSPYTTDLQRTRLILPSAKIDTYT 167
>SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1666
Score = 29.9 bits (64), Expect = 0.36
Identities = 27/110 (24%), Positives = 49/110 (44%), Gaps = 5/110 (4%)
Frame = +1
Query: 196 VDISTQLVKVVSKIVLENSGKSSVKH-----FLFAVEEAAKNNLAFIGAKDSNSKDLRLA 360
VD+ +QL++++ KIVL+ S S + FL A++ + +I D D +A
Sbjct: 994 VDLPSQLIELLEKIVLQPSSFSENANLQNLLFLTAIKADKSRVMEYIDKLDKYDVD-EIA 1052
Query: 361 ETTVKGYDYVKFWRVELKEPLNAGATVNVVVETVFTKALQPYPTAITQQE 510
E ++ Y + +R+ + A +V + V Q Y + Q E
Sbjct: 1053 EIAIENGLYEEAFRIYKIHNKHEQAMKVLVEDIVSLDRAQDYAETVEQPE 1102
>SPBC12C2.05c |||diacylglycerol binding protein Bzz1
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 642
Score = 28.7 bits (61), Expect = 0.83
Identities = 17/42 (40%), Positives = 25/42 (59%)
Frame = +1
Query: 535 NLYTYSPYYTSTQKTSVILNTKTVESFTKVKPFSQVDGTIIY 660
N YT +S +TS + N KT E+ T +P S+VDG ++Y
Sbjct: 485 NTYTDDMDSSSIYQTS-LSNVKTEET-TPAEPASKVDGVVLY 524
>SPAC22H10.10 |alp21|sto1|tubulin specific chaperone cofactor
E|Schizosaccharomyces pombe|chr 1|||Manual
Length = 511
Score = 26.6 bits (56), Expect = 3.4
Identities = 14/27 (51%), Positives = 17/27 (62%), Gaps = 1/27 (3%)
Frame = +1
Query: 556 YYTSTQKTSVILNT-KTVESFTKVKPF 633
YYTS + VI N K VE TK++PF
Sbjct: 364 YYTSCIRKLVIDNEIKDVEELTKIEPF 390
>SPBC3E7.01 |fab1|ste12,
SPBC6B1.11c|1-phosphatidylinositol-3-phosphate 5-kinase
Fab1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 1932
Score = 25.8 bits (54), Expect = 5.9
Identities = 17/34 (50%), Positives = 19/34 (55%)
Frame = +1
Query: 160 SSDIKLKNVDRTVDISTQLVKVVSKIVLENSGKS 261
SSDI N+D T DISTQ S + NSG S
Sbjct: 1303 SSDIPKANIDFTNDISTQNTFPASPV--SNSGFS 1334
>SPCC550.04c |gpi2||pig-C|Schizosaccharomyces pombe|chr 3|||Manual
Length = 324
Score = 25.8 bits (54), Expect = 5.9
Identities = 9/21 (42%), Positives = 14/21 (66%)
Frame = -2
Query: 543 IQIANIFHKLVFLLSYSCRIW 481
+ IA +F L+F +S+ C IW
Sbjct: 277 VVIAFVFLSLIFFISFICPIW 297
>SPAC227.13c |isu1||mitochondrial iron-sulfur cluster assembly
scaffold protein Isu1|Schizosaccharomyces pombe|chr
1|||Manual
Length = 192
Score = 25.8 bits (54), Expect = 5.9
Identities = 12/41 (29%), Positives = 23/41 (56%)
Frame = +1
Query: 154 TISSDIKLKNVDRTVDISTQLVKVVSKIVLENSGKSSVKHF 276
T+ K+KN ++ VK+ ++ E++ KS+VKH+
Sbjct: 132 TLEEASKIKNTQIAKELCLPPVKLHCSMLAEDAIKSAVKHY 172
>SPAC6F6.06c |rax2||cell polarity factor Rax2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1155
Score = 25.8 bits (54), Expect = 5.9
Identities = 20/68 (29%), Positives = 31/68 (45%), Gaps = 8/68 (11%)
Frame = +1
Query: 508 EDQFVKYVGNLYTYS--PYYTSTQKTSVILNTKTVESFTKVKP------FSQVDGTIIYG 663
+D+F Y +T+S P +TS + +V + T+E F VK F D I G
Sbjct: 131 DDKF-PYAYATFTFSDKPSFTSIYRWNVTNSNVTIEHFYNVKGNVDSLFFLNNDSVAISG 189
Query: 664 PYANVGPF 687
+ + PF
Sbjct: 190 NFTEISPF 197
>SPAC17C9.07 |alg8||glucosyltransferase Alg8|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 501
Score = 25.4 bits (53), Expect = 7.8
Identities = 16/51 (31%), Positives = 25/51 (49%)
Frame = -3
Query: 248 FSKTIFDTTFTSWVDISTVRSTFFNFISLDIVSTLALRVCIVRNNTKKSVN 96
F K + D ++V STV + I L++V ALR ++ +N K N
Sbjct: 80 FDKAMLDPYNLNYVSPSTVVFQRGSVIVLELVLLFALREYVLSSNVKDQRN 130
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,880,564
Number of Sequences: 5004
Number of extensions: 61665
Number of successful extensions: 175
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 169
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 174
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 317927284
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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