Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmmt25n06
(693 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC1198.08 |||dipeptidase Dug1 |Schizosaccharomyces pombe|chr 2... 180 2e-46
SPAC24C9.08 |||vacuolar carboxypeptidase |Schizosaccharomyces po... 37 0.003
SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 31 0.21
SPBC19C7.08c |||leucine carboxyl methyltransferase|Schizosacchar... 26 4.5
SPAC20G8.09c |||N-acetyltransferase Nat10 |Schizosaccharomyces p... 26 5.9
SPBC1289.10c |||transcription factor |Schizosaccharomyces pombe|... 25 7.9
SPBC16D10.05 |mok13||alpha-1,3-glucan synthase Mok13|Schizosacch... 25 7.9
SPAC222.13c |||6-phosphofructo-2-kinase |Schizosaccharomyces pom... 25 7.9
SPBC17A3.01c |tim50|SPBC8D2.21c|TIM23 translocase complex subuni... 25 7.9
>SPBC1198.08 |||dipeptidase Dug1 |Schizosaccharomyces pombe|chr
2|||Manual
Length = 474
Score = 180 bits (437), Expect = 2e-46
Identities = 85/194 (43%), Positives = 120/194 (61%), Gaps = 1/194 (0%)
Frame = +2
Query: 113 TLPEIFKYVDQNKDSYKQLLKEAVAIPSVSCDVKYRADCIRMVHWMQDKLKEVGATTELR 292
+L ++++ +D+ KD + L AV+IPSVS DV R + M ++ + ++GA E R
Sbjct: 2 SLDKLYEVIDKKKDEFVTRLSRAVSIPSVSADVTLRPKVVEMADFVVSEFTKLGAKMEKR 61
Query: 293 DVGFQTIDGKDVQXXXXXXXXXXNDPKKNTVCIYGHLDVQPALKSDGWETEPFEL-VERN 469
D+G+ +DG+DV NDP K TV IY H DVQPA DGW T+PF L V+
Sbjct: 62 DIGYHQMDGQDVPLPPIVLGQYGNDPSKKTVLIYNHFDVQPASLEDGWSTDPFTLTVDNK 121
Query: 470 EKLYGRGSTDDKGPVLGWLHTINAYKGTGAELPVNLKFIFECMEESGSEGLDSLLMDKLK 649
+++GRG+TDDKGP++GW+ I A+K G + PVNL FE MEE GSEGL+ L+ + +
Sbjct: 122 GRMFGRGATDDKGPLIGWISAIEAHKELGIDFPVNLLMCFEGMEEYGSEGLEDLI--RAE 179
Query: 650 PEGFFDSVDYVCIS 691
E +F D VCIS
Sbjct: 180 AEKYFAKADCVCIS 193
>SPAC24C9.08 |||vacuolar carboxypeptidase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 596
Score = 36.7 bits (81), Expect = 0.003
Identities = 18/51 (35%), Positives = 25/51 (49%), Gaps = 2/51 (3%)
Frame = +2
Query: 389 IYGHLDVQPALKS--DGWETEPFELVERNEKLYGRGSTDDKGPVLGWLHTI 535
+ GH DV P ++ D W PF N +Y RG+ DDK V+ L +
Sbjct: 194 LMGHQDVVPVNQASLDRWYFPPFSATYHNGHVYSRGAADDKNSVVAILEAL 244
>SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 534
Score = 30.7 bits (66), Expect = 0.21
Identities = 32/120 (26%), Positives = 50/120 (41%), Gaps = 3/120 (2%)
Frame = -1
Query: 525 SQPSTGPLSSVEPLPYNFSLRSTSSKGSVSHPSDFNAGCTSK*P*IQTVFFFGSLPNTPT 346
S T S +P S S++S S S A +S I + S ++PT
Sbjct: 227 SSTLTSSSLSTSSIPSTSSSSSSTSSSLSSSSSSSTASSSSSSSSI--ISSSSSSSSSPT 284
Query: 345 KTGGS*TSLPSIV*KPTSLNSVVAPTSFNLSCIQ*TI---RMQSARYFTSHETDGMATAS 175
T + +S S PTS +S ++ +S + S T+ M S+ F+S T +T S
Sbjct: 285 STSSTISSSSSSSSSPTSTSSTISSSSSSSSSFSSTLSSSSMSSSSSFSSSPTSSSSTIS 344
>SPBC19C7.08c |||leucine carboxyl
methyltransferase|Schizosaccharomyces pombe|chr
2|||Manual
Length = 681
Score = 26.2 bits (55), Expect = 4.5
Identities = 10/22 (45%), Positives = 14/22 (63%)
Frame = -2
Query: 416 QVVHPNDHRYKLYFFLDHYPIR 351
++V P D + YFFL HY I+
Sbjct: 295 EMVEPFDEWEEFYFFLQHYSIQ 316
>SPAC20G8.09c |||N-acetyltransferase Nat10 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1033
Score = 25.8 bits (54), Expect = 5.9
Identities = 15/35 (42%), Positives = 22/35 (62%), Gaps = 1/35 (2%)
Frame = +2
Query: 506 GPVLGWL-HTINAYKGTGAELPVNLKFIFECMEES 607
GP L ++ TIN Y+GTG L +LK + + E+S
Sbjct: 401 GPYLVFMASTINGYEGTGRSL--SLKLLQQLREQS 433
>SPBC1289.10c |||transcription factor |Schizosaccharomyces pombe|chr
2|||Manual
Length = 743
Score = 25.4 bits (53), Expect = 7.9
Identities = 14/32 (43%), Positives = 19/32 (59%)
Frame = +3
Query: 405 MYNLH*NLMDGRLNLLN*LSAMKNYMEEVLLM 500
+YN + N RLN A+KNYMEE+ L+
Sbjct: 435 LYNFNGNANPSRLN-----PALKNYMEELKLL 461
>SPBC16D10.05 |mok13||alpha-1,3-glucan synthase
Mok13|Schizosaccharomyces pombe|chr 2|||Manual
Length = 2358
Score = 25.4 bits (53), Expect = 7.9
Identities = 10/28 (35%), Positives = 16/28 (57%)
Frame = -2
Query: 473 FHCAQLVQKVQSPIHQISMQVVHPNDHR 390
FH + + + IH+I +Q VH DH+
Sbjct: 796 FHWSGDLINIADGIHEIKLQRVHSQDHQ 823
>SPAC222.13c |||6-phosphofructo-2-kinase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 592
Score = 25.4 bits (53), Expect = 7.9
Identities = 14/39 (35%), Positives = 21/39 (53%)
Frame = -1
Query: 486 LPYNFSLRSTSSKGSVSHPSDFNAGCTSK*P*IQTVFFF 370
L Y L ST+ + S+S S FN + + I+T+ FF
Sbjct: 406 LRYTNDLASTNKRFSLSEASSFNVWSSVRKRAIETIEFF 444
>SPBC17A3.01c |tim50|SPBC8D2.21c|TIM23 translocase complex subunit
Tim50 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 452
Score = 25.4 bits (53), Expect = 7.9
Identities = 14/37 (37%), Positives = 17/37 (45%)
Frame = +2
Query: 368 PKKNTVCIYGHLDVQPALKSDGWETEPFELVERNEKL 478
P + I DV+P LKS + P E R EKL
Sbjct: 311 PLLEFIAIMDIKDVRPVLKSYQGKNIPLEYARREEKL 347
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,039,701
Number of Sequences: 5004
Number of extensions: 64434
Number of successful extensions: 192
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 183
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 191
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 321951680
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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