Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= bmmt25l06
         (704 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC31G5.16c |dpm1||dolichol-phosphate mannosyltransferase catal...   315   5e-87
SPBC56F2.10c |alg5||dolichyl-phosphate beta-glucosyltransferase ...    50   2e-07
SPBC27.02c |ask1|mug181|DASH complex subunit Ask1|Schizosaccharo...    29   0.86 
SPBC12C2.07c |||spermidine synthase |Schizosaccharomyces pombe|c...    27   2.0  
SPBC1685.14c |||Vid27 family protein|Schizosaccharomyces pombe|c...    27   2.6  
SPAC29A4.15 |||serine-tRNA ligase|Schizosaccharomyces pombe|chr ...    27   3.5  

>SPAC31G5.16c |dpm1||dolichol-phosphate mannosyltransferase
           catalytic subunit Dpm1|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 236

 Score =  315 bits (773), Expect = 5e-87
 Identities = 143/211 (67%), Positives = 176/211 (83%)
 Frame = +1

Query: 70  KYSILLPTYNERENLPIIIWLIIKYLDESGVDYEVIIIDDGSPDGTSEVARQLQKLYGSS 249
           KYS+LLPTYNER+NLPII +LI K  D+  +D+E++IIDD SPDGT EVA++LQK+YG  
Sbjct: 3   KYSVLLPTYNERKNLPIITYLIAKTFDQEKLDWEIVIIDDASPDGTQEVAKELQKIYGED 62

Query: 250 KIVLRPREMKLGLGTAYIHGIQQASGNFIIIMDADLSHHPKFIPEFIKLQLKYDYDIVSG 429
           KI+L+PR  KLGLGTAYIHG++ A+G+F+IIMDAD SHHPK++PEFIKLQ +++YDIV G
Sbjct: 63  KILLKPRSGKLGLGTAYIHGLKFATGDFVIIMDADFSHHPKYLPEFIKLQKEHNYDIVLG 122

Query: 430 TRYKGSGGVYGWDFKRKLISRGANFLTQLMLRPGVSDLTGSFRLYKKEVLEKLILSCVSK 609
           TRY   GGVYGW+ KRK ISRGAN L   +L  GVSD+TGSFRLYKK VLE L+    SK
Sbjct: 123 TRYAKDGGVYGWNLKRKFISRGANLLASTVLGTGVSDVTGSFRLYKKPVLETLMSEVTSK 182

Query: 610 GYVFQMEMIIRARQFDYSIGEVPISFVDRVY 702
           GYVFQME+I RAR+ +Y+IGEVPI+FVDR+Y
Sbjct: 183 GYVFQMEIIARAREHNYTIGEVPIAFVDRLY 213


>SPBC56F2.10c |alg5||dolichyl-phosphate beta-glucosyltransferase
           Alg5 |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 322

 Score = 50.4 bits (115), Expect = 2e-07
 Identities = 50/216 (23%), Positives = 95/216 (43%), Gaps = 12/216 (5%)
 Frame = +1

Query: 76  SILLPTYNERENLPIIIWLIIKYLDE-------SGVD-YEVIIIDDGSPDGTSEVARQLQ 231
           ++++P YNE + +  ++   + +L++       +G   +E++I+DD S D T     +  
Sbjct: 65  TVIVPAYNESKRIGNMLQETVDHLEKYYRSSSSAGQRRWEILIVDDESKDTTVNAVLEFS 124

Query: 232 -KLYGSSKIVLRPREMKLGLGTAYIHGIQQASGNFIIIMDAD-LSHHPKFIPEFIKLQLK 405
            KL     + +   +   G G A   G+  A G + I  DAD  S        F  +   
Sbjct: 125 NKLDLRDHLRVCSLKRNRGKGGAVTWGMLYARGQYAIFADADGASQFSDLELLFKNMPPG 184

Query: 406 YDYDIVSGTRYK--GSGGVYGWDFKRKLISRGANFLTQLMLRPGVSDLTGSFRLYKKEVL 579
               +V G+R     +  V    F R  +    + L Q++    + D    F+L+ +E  
Sbjct: 185 PRGGVVVGSRAHMVNTAAVVKRSFIRNFLMHCFHKLLQILGIREIGDTQCGFKLFSREAY 244

Query: 580 EKLILSCVSKGYVFQMEMIIRARQFDYSIGEVPISF 687
           + +      +G++F +E++  AR F   I EVPI++
Sbjct: 245 QSIFPRMHVEGWIFDIEVLTLARFFGLPIIEVPITW 280


>SPBC27.02c |ask1|mug181|DASH complex subunit
           Ask1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 307

 Score = 28.7 bits (61), Expect = 0.86
 Identities = 8/21 (38%), Positives = 15/21 (71%)
 Frame = +3

Query: 276 EAWSRNCLHSWDTASFWKFYY 338
           E +++NC   WD++ FWK ++
Sbjct: 41  EKYAKNCNTIWDSSKFWKQFF 61


>SPBC12C2.07c |||spermidine synthase |Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 298

 Score = 27.5 bits (58), Expect = 2.0
 Identities = 22/72 (30%), Positives = 33/72 (45%), Gaps = 3/72 (4%)
 Frame = +1

Query: 19  VETIQVMEVDSGLIKRDKYSILLPTYNERENLPIIIWLI---IKYLDESGVDYEVIIIDD 189
           VE   + ++D  +IK  K    LP  +   N P +   I    K+L +    ++VII D 
Sbjct: 112 VEEAILCDIDEDVIKVSKQ--YLPEMSAGFNHPKVKVHIGDGFKFLQDYQNTFDVIITDS 169

Query: 190 GSPDGTSEVARQ 225
             PDG +E   Q
Sbjct: 170 SDPDGPAEALFQ 181


>SPBC1685.14c |||Vid27 family protein|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 801

 Score = 27.1 bits (57), Expect = 2.6
 Identities = 23/85 (27%), Positives = 34/85 (40%), Gaps = 1/85 (1%)
 Frame = +3

Query: 201 WDFRSGASAAEALWLL*NSASTSRDEAWSRNCLHSWDTASF-WKFYYYNGCGFEPSSKVH 377
           W++    S  E  WL   S S   D     N + S++  SF W ++  N   F    +  
Sbjct: 279 WNYWFTISTEEKTWL---SQSVDAD----MNPVFSFEHLSFIWNYFDANSNAFSWLLRFD 331

Query: 378 SRVHQTPIKI*LRYCLWYSLQGEWW 452
           S+V     +  L   LW SL  + W
Sbjct: 332 SQVRMEQFQELLMRALWESLNQQRW 356


>SPAC29A4.15 |||serine-tRNA ligase|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 450

 Score = 26.6 bits (56), Expect = 3.5
 Identities = 17/51 (33%), Positives = 25/51 (49%)
 Frame = +1

Query: 538 DLTGSFRLYKKEVLEKLILSCVSKGYVFQMEMIIRARQFDYSIGEVPISFV 690
           D  G FR++  E +E+ +L+   K +    EMI  A  F Y   E+P   V
Sbjct: 280 DAWGIFRVHAFEKIEQFVLTDPEKSWEAFTEMINHAEDF-YKSLELPYRIV 329


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,956,274
Number of Sequences: 5004
Number of extensions: 59547
Number of successful extensions: 143
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 142
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 143
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 327172622
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)