BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmmt25g02
(664 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC14F5.07 |||ER-localized ubiquitin ligase |Schizosaccharomyce... 27 1.8
SPBC106.10 |pka1|tpk, git6|cAMP-dependent protein kinase catalyt... 27 3.2
SPCC70.03c |||proline dehydrogenase|Schizosaccharomyces pombe|ch... 27 3.2
SPAC343.18 |rfp2||ubiquitin-protein ligase E3 Rfp2|Schizosacchar... 26 5.6
SPBC29A10.04 |psm1|smc1|mitotic cohesin complex subunit Psm1 |Sc... 25 9.7
>SPBC14F5.07 |||ER-localized ubiquitin ligase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1242
Score = 27.5 bits (58), Expect = 1.8
Identities = 23/76 (30%), Positives = 39/76 (51%), Gaps = 6/76 (7%)
Frame = +3
Query: 330 FYLHRIPKFYL*F--FRLHLAVDYLYIDYLEKLLNV--SCVVIKSLVC*FLEKKYFYI*L 497
FY + I + + F + +H +V +L +DYL L+N +V++SLV + KY +
Sbjct: 1003 FYAYAIGFYSISFPMYAIHASVKFLKLDYLRSLMNKLNLKIVMRSLV---MALKYLLLAF 1059
Query: 498 WWSNLLGLFVG--WSL 539
+L L +G W L
Sbjct: 1060 LGIFILPLLLGAIWEL 1075
>SPBC106.10 |pka1|tpk, git6|cAMP-dependent protein kinase catalytic
subunit Pka1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 512
Score = 26.6 bits (56), Expect = 3.2
Identities = 22/71 (30%), Positives = 33/71 (46%), Gaps = 1/71 (1%)
Frame = -2
Query: 528 RQTSPAD*TTTTRCKNIFFQEISKQGT*LQHKTHLTIFPNNLYIDSRQLNEAEKIID-KI 352
R+ PAD TT R F + GT + HL +N + ++ E +KI+D K
Sbjct: 183 RRIRPADHTTKDRYGIQDFNFLQTLGTGSFGRVHLVQSNHNRLYYAIKVLEKKKIVDMKQ 242
Query: 351 LESGADRKIIL 319
+E D + IL
Sbjct: 243 IEHTCDERYIL 253
>SPCC70.03c |||proline dehydrogenase|Schizosaccharomyces pombe|chr
3|||Manual
Length = 492
Score = 26.6 bits (56), Expect = 3.2
Identities = 9/31 (29%), Positives = 20/31 (64%)
Frame = +1
Query: 1 SITTSPITKKLKSIFIIHNTHRICLNEQKKV 93
++T + K K + I+HNT+++ L + +K+
Sbjct: 279 AVTVDLMRKYNKEVAIVHNTYQLYLKKSRKI 309
>SPAC343.18 |rfp2||ubiquitin-protein ligase E3
Rfp2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 205
Score = 25.8 bits (54), Expect = 5.6
Identities = 9/17 (52%), Positives = 11/17 (64%)
Frame = +1
Query: 472 KKNIFTSSCGGLICWAC 522
KK+IF + CG L C C
Sbjct: 159 KKSIFAAKCGHLFCSTC 175
>SPBC29A10.04 |psm1|smc1|mitotic cohesin complex subunit Psm1
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1233
Score = 25.0 bits (52), Expect = 9.7
Identities = 15/38 (39%), Positives = 23/38 (60%), Gaps = 1/38 (2%)
Frame = -2
Query: 441 QHKTHLTIFPNNLYIDSRQLNEA-EKIIDKILESGADR 331
Q K LT + L+ +++LNE + + KILE+ ADR
Sbjct: 464 QQKKRLTY--SELFHKTQELNEELQSCLQKILEASADR 499
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,338,210
Number of Sequences: 5004
Number of extensions: 44216
Number of successful extensions: 101
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 95
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 100
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 301829700
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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