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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= bmmt25g02
         (664 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC14F5.07 |||ER-localized ubiquitin ligase |Schizosaccharomyce...    27   1.8  
SPBC106.10 |pka1|tpk, git6|cAMP-dependent protein kinase catalyt...    27   3.2  
SPCC70.03c |||proline dehydrogenase|Schizosaccharomyces pombe|ch...    27   3.2  
SPAC343.18 |rfp2||ubiquitin-protein ligase E3 Rfp2|Schizosacchar...    26   5.6  
SPBC29A10.04 |psm1|smc1|mitotic cohesin complex subunit Psm1 |Sc...    25   9.7  

>SPBC14F5.07 |||ER-localized ubiquitin ligase |Schizosaccharomyces
            pombe|chr 2|||Manual
          Length = 1242

 Score = 27.5 bits (58), Expect = 1.8
 Identities = 23/76 (30%), Positives = 39/76 (51%), Gaps = 6/76 (7%)
 Frame = +3

Query: 330  FYLHRIPKFYL*F--FRLHLAVDYLYIDYLEKLLNV--SCVVIKSLVC*FLEKKYFYI*L 497
            FY + I  + + F  + +H +V +L +DYL  L+N     +V++SLV   +  KY  +  
Sbjct: 1003 FYAYAIGFYSISFPMYAIHASVKFLKLDYLRSLMNKLNLKIVMRSLV---MALKYLLLAF 1059

Query: 498  WWSNLLGLFVG--WSL 539
                +L L +G  W L
Sbjct: 1060 LGIFILPLLLGAIWEL 1075


>SPBC106.10 |pka1|tpk, git6|cAMP-dependent protein kinase catalytic
           subunit Pka1 |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 512

 Score = 26.6 bits (56), Expect = 3.2
 Identities = 22/71 (30%), Positives = 33/71 (46%), Gaps = 1/71 (1%)
 Frame = -2

Query: 528 RQTSPAD*TTTTRCKNIFFQEISKQGT*LQHKTHLTIFPNNLYIDSRQLNEAEKIID-KI 352
           R+  PAD TT  R     F  +   GT    + HL    +N    + ++ E +KI+D K 
Sbjct: 183 RRIRPADHTTKDRYGIQDFNFLQTLGTGSFGRVHLVQSNHNRLYYAIKVLEKKKIVDMKQ 242

Query: 351 LESGADRKIIL 319
           +E   D + IL
Sbjct: 243 IEHTCDERYIL 253


>SPCC70.03c |||proline dehydrogenase|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 492

 Score = 26.6 bits (56), Expect = 3.2
 Identities = 9/31 (29%), Positives = 20/31 (64%)
 Frame = +1

Query: 1   SITTSPITKKLKSIFIIHNTHRICLNEQKKV 93
           ++T   + K  K + I+HNT+++ L + +K+
Sbjct: 279 AVTVDLMRKYNKEVAIVHNTYQLYLKKSRKI 309


>SPAC343.18 |rfp2||ubiquitin-protein ligase E3
           Rfp2|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 205

 Score = 25.8 bits (54), Expect = 5.6
 Identities = 9/17 (52%), Positives = 11/17 (64%)
 Frame = +1

Query: 472 KKNIFTSSCGGLICWAC 522
           KK+IF + CG L C  C
Sbjct: 159 KKSIFAAKCGHLFCSTC 175


>SPBC29A10.04 |psm1|smc1|mitotic cohesin complex subunit Psm1
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1233

 Score = 25.0 bits (52), Expect = 9.7
 Identities = 15/38 (39%), Positives = 23/38 (60%), Gaps = 1/38 (2%)
 Frame = -2

Query: 441 QHKTHLTIFPNNLYIDSRQLNEA-EKIIDKILESGADR 331
           Q K  LT   + L+  +++LNE  +  + KILE+ ADR
Sbjct: 464 QQKKRLTY--SELFHKTQELNEELQSCLQKILEASADR 499


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,338,210
Number of Sequences: 5004
Number of extensions: 44216
Number of successful extensions: 101
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 95
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 100
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 301829700
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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