BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmmt25f22
(611 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC365.04c |||RNA-binding protein|Schizosaccharomyces pombe|chr... 31 0.17
SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma... 30 0.30
SPCC1827.07c ||SPCP1E11.01c|SPX/EXS domain protein|Schizosacchar... 28 0.93
SPAC17G8.03c |dpb3||DNA polymerase epsilon subunit Dpb3|Schizosa... 28 0.93
SPBC32F12.05c |cwf12||complexed with Cdc5 protein Cwf12 |Schizos... 27 2.1
SPAC637.11 |suv3||ATP-dependent RNA helicase Suv3|Schizosaccharo... 27 2.1
SPAC29E6.09 ||SPAC30.13|sequence orphan|Schizosaccharomyces pomb... 25 6.5
SPCC1223.01 ||SPCC285.18|ubiquitin-protein ligase E3 |Schizosacc... 25 8.6
>SPBC365.04c |||RNA-binding protein|Schizosaccharomyces pombe|chr
2|||Manual
Length = 233
Score = 30.7 bits (66), Expect = 0.17
Identities = 30/122 (24%), Positives = 55/122 (45%), Gaps = 5/122 (4%)
Frame = +2
Query: 47 LEANAKEFDSVLKLYPQAIKLKAERKTKRPDELIK--LDNWYQNELPKK--IKSRGKDAH 214
LE +K+FD Q+++ ++ ++ +EL K D ++ELP+K +S KD H
Sbjct: 13 LEYRSKKFDKK----SQSLEEHEKKVQQKNEELEKKAADKISRDELPEKQLAQSNDKDKH 68
Query: 215 MIHEELVQLMKWKQARGKFYPQLSYLIKVNTPRAVMQETKKA-FRKLPNIESAMTALSNL 391
+ + +K K+ +GK + L N P+ ET + F++ + S
Sbjct: 69 SVSNPPHKTLKSKRQKGKNNDRKVILFVGNLPKDSSVETLQLHFKRAGQVPSVRIPTDKT 128
Query: 392 KG 397
G
Sbjct: 129 SG 130
>SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr
3|||Manual
Length = 176
Score = 29.9 bits (64), Expect = 0.30
Identities = 21/81 (25%), Positives = 43/81 (53%), Gaps = 4/81 (4%)
Frame = +2
Query: 170 NELPKKIKSRGKDAHMIHEELVQLMKWKQARGKFYPQLSYLIKVNTPRAV----MQETKK 337
+EL +++ G +A E++++++ GK Y Q+ ++V T + V ++E K+
Sbjct: 57 HELRAAMRALGFNAEK--SEVLKILRDFDKTGKGYLQMEDFVRVMTEKIVERDPLEEIKR 114
Query: 338 AFRKLPNIESAMTALSNLKGV 400
AF + E+ +L NL+ V
Sbjct: 115 AFELFDDDETGKISLRNLRRV 135
>SPCC1827.07c ||SPCP1E11.01c|SPX/EXS domain
protein|Schizosaccharomyces pombe|chr 3|||Manual
Length = 682
Score = 28.3 bits (60), Expect = 0.93
Identities = 15/33 (45%), Positives = 17/33 (51%)
Frame = -2
Query: 310 WRVHFYQIRQLRVEFSSGLFPFHELDKFFVDHV 212
WR+ Y I QL F SGL H D FF D +
Sbjct: 401 WRMRRYLIIQLIRVFLSGLSTVHFQDFFFADQM 433
>SPAC17G8.03c |dpb3||DNA polymerase epsilon subunit
Dpb3|Schizosaccharomyces pombe|chr 1|||Manual
Length = 199
Score = 28.3 bits (60), Expect = 0.93
Identities = 19/51 (37%), Positives = 29/51 (56%), Gaps = 5/51 (9%)
Frame = +2
Query: 56 NAKEFD---SVLKLYPQAIKLKAERKTKRPDELIKLDNWYQNE-LP-KKIK 193
+ ++FD +++ P A +KAERKTKRP + NE +P KK+K
Sbjct: 88 SVEQFDFLQDIVEKVPDAPPIKAERKTKRPRARRAANEGEHNESVPAKKVK 138
>SPBC32F12.05c |cwf12||complexed with Cdc5 protein Cwf12
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 217
Score = 27.1 bits (57), Expect = 2.1
Identities = 17/67 (25%), Positives = 29/67 (43%)
Frame = +2
Query: 29 DTSTFFLEANAKEFDSVLKLYPQAIKLKAERKTKRPDELIKLDNWYQNELPKKIKSRGKD 208
D + A+E V +L+ + ER+ K+ + +LD WY +P +S +D
Sbjct: 103 DIDDYRYYGRARELPGVKELFEADMSFIPERQRKQEMQKRRLDAWYFGYIPPAQESLLED 162
Query: 209 AHMIHEE 229
EE
Sbjct: 163 FEAKIEE 169
>SPAC637.11 |suv3||ATP-dependent RNA helicase
Suv3|Schizosaccharomyces pombe|chr 1|||Manual
Length = 647
Score = 27.1 bits (57), Expect = 2.1
Identities = 18/58 (31%), Positives = 30/58 (51%), Gaps = 6/58 (10%)
Frame = +2
Query: 11 TMATAKDTSTFFLEANAKEFDSVLKLYPQAIKLKAERKTKRPD---ELIKLDN---WY 166
++A +K +STF L+ K D +L Y + ++ + + RPD +L L N WY
Sbjct: 107 SLALSKSSSTFDLQKIKKIHDCLLSEYRKYVRYQERIEETRPDLQKQLTDLKNPIEWY 164
>SPAC29E6.09 ||SPAC30.13|sequence orphan|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 339
Score = 25.4 bits (53), Expect = 6.5
Identities = 18/71 (25%), Positives = 34/71 (47%)
Frame = +2
Query: 170 NELPKKIKSRGKDAHMIHEELVQLMKWKQARGKFYPQLSYLIKVNTPRAVMQETKKAFRK 349
+ELP+ I +G + E+L L QA + Y + + + ++ K F+K
Sbjct: 3 SELPEVI-FKGTE---FEEQLSNLTSDSQANKRVYEDYDFKDETSAKPIPTEKKLKVFKK 58
Query: 350 LPNIESAMTAL 382
N+E+ ++AL
Sbjct: 59 DDNVETLLSAL 69
>SPCC1223.01 ||SPCC285.18|ubiquitin-protein ligase E3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 732
Score = 25.0 bits (52), Expect = 8.6
Identities = 15/43 (34%), Positives = 20/43 (46%)
Frame = +2
Query: 110 KAERKTKRPDELIKLDNWYQNELPKKIKSRGKDAHMIHEELVQ 238
+ ER + P E N N L SRG+ AH+ EE V+
Sbjct: 361 RRERTSSTPSEQSTSVNETANSLSNLHLSRGEIAHLRQEEYVR 403
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,663,302
Number of Sequences: 5004
Number of extensions: 55762
Number of successful extensions: 151
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 149
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 151
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 267622334
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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