BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmmt25d04
(500 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC664.14 |amt2||ammonium transporter Amt2|Schizosaccharomyces ... 27 2.1
SPBPB10D8.02c |||arylsulfatase |Schizosaccharomyces pombe|chr 2|... 26 2.8
SPCC1620.04c |mug55||Cdc20/Fizzy family WD repeat protein|Schizo... 26 3.7
SPCC1753.02c |git3||G-protein coupled receptor Git3|Schizosaccha... 25 4.8
SPCC965.06 |||potassium channel subunit |Schizosaccharomyces pom... 25 6.4
SPAPB15E9.02c |||dubious|Schizosaccharomyces pombe|chr 1|||Manual 25 6.4
SPBC1348.10c |||phospholipase |Schizosaccharomyces pombe|chr 2||... 25 8.4
SPAC977.09c |||phospholipase |Schizosaccharomyces pombe|chr 1|||... 25 8.4
>SPAC664.14 |amt2||ammonium transporter Amt2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 512
Score = 26.6 bits (56), Expect = 2.1
Identities = 12/40 (30%), Positives = 19/40 (47%)
Frame = +2
Query: 128 AKKGVVAFVKDFYYDPFKWSLVKSVGFFTVGVVIASECTG 247
A G+V + D+ + KWS + G+V A+ C G
Sbjct: 280 AVAGLVWVIYDYIFRTRKWSTIGFCSGVVAGLVAATPCAG 319
>SPBPB10D8.02c |||arylsulfatase |Schizosaccharomyces pombe|chr
2|||Manual
Length = 554
Score = 26.2 bits (55), Expect = 2.8
Identities = 10/18 (55%), Positives = 14/18 (77%)
Frame = +2
Query: 350 NAVAELKRPNFVLILTDD 403
N AE K+PNF++I+ DD
Sbjct: 4 NKQAESKKPNFLVIVADD 21
>SPCC1620.04c |mug55||Cdc20/Fizzy family WD repeat
protein|Schizosaccharomyces pombe|chr 3|||Manual
Length = 509
Score = 25.8 bits (54), Expect = 3.7
Identities = 17/65 (26%), Positives = 24/65 (36%), Gaps = 1/65 (1%)
Frame = +2
Query: 80 SSHSQQKQLPLFELIMAKKGVVAFVKDFYYDPFKWSLVKSVGF-FTVGVVIASECTGLEI 256
+ H Q L+E + + DFY P WS + V + SE +G I
Sbjct: 135 NGHLSNMQGSLYETPLRILDAPGLLDDFYISPLAWSTNGELAVALAQNVYLWSEISGPSI 194
Query: 257 MPAMP 271
M P
Sbjct: 195 MELSP 199
>SPCC1753.02c |git3||G-protein coupled receptor
Git3|Schizosaccharomyces pombe|chr 3|||Manual
Length = 466
Score = 25.4 bits (53), Expect = 4.8
Identities = 10/25 (40%), Positives = 14/25 (56%)
Frame = -1
Query: 383 QSLVVLVQPQHCSRKILKINIVTLF 309
Q +V+L QP HCS + V +F
Sbjct: 388 QIIVILAQPLHCSGSCKRFAFVAVF 412
>SPCC965.06 |||potassium channel subunit |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 344
Score = 25.0 bits (52), Expect = 6.4
Identities = 10/38 (26%), Positives = 20/38 (52%)
Frame = +2
Query: 74 IISSHSQQKQLPLFELIMAKKGVVAFVKDFYYDPFKWS 187
+I +H +P+ E++ A ++ K FY+ +WS
Sbjct: 133 VIMAHRPDPSVPMEEVVRAFTQLIQDGKAFYWGTSEWS 170
>SPAPB15E9.02c |||dubious|Schizosaccharomyces pombe|chr 1|||Manual
Length = 188
Score = 25.0 bits (52), Expect = 6.4
Identities = 9/20 (45%), Positives = 12/20 (60%)
Frame = -2
Query: 499 FFFTFMYNQFILSHCNFFYI 440
FF+ F + F SHC F+I
Sbjct: 74 FFYWFFFFFFFFSHCRRFHI 93
>SPBC1348.10c |||phospholipase |Schizosaccharomyces pombe|chr
2|||Manual
Length = 673
Score = 24.6 bits (51), Expect = 8.4
Identities = 13/45 (28%), Positives = 24/45 (53%), Gaps = 2/45 (4%)
Frame = +2
Query: 74 IISSHSQQKQLPLFELIMAKKGV-VAFVKDFYY-DPFKWSLVKSV 202
++ ++ +PL+ L+ ++ V V F D Y DP+ W L S+
Sbjct: 424 LVDGGEDRENIPLWPLLHPQRFVDVVFAIDSTYNDPYGWPLGSSI 468
>SPAC977.09c |||phospholipase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 673
Score = 24.6 bits (51), Expect = 8.4
Identities = 13/45 (28%), Positives = 24/45 (53%), Gaps = 2/45 (4%)
Frame = +2
Query: 74 IISSHSQQKQLPLFELIMAKKGV-VAFVKDFYY-DPFKWSLVKSV 202
++ ++ +PL+ L+ ++ V V F D Y DP+ W L S+
Sbjct: 424 LVDGGEDRENIPLWPLLHPQRFVDVVFAIDSTYNDPYGWPLGSSI 468
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,856,253
Number of Sequences: 5004
Number of extensions: 34258
Number of successful extensions: 67
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 67
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 67
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 198176188
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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