Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmmt25b01
(534 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC21.05c |ral2||Ras guanyl-nucleotide exchange factor Ral2 |Sc... 30 0.25
SPCC188.02 |par1||protein phosphatase regulatory subunit Par1 |S... 29 0.58
SPCC895.06 |||RNA polymerase II elongator complex subunit Elp2 |... 29 0.58
SPAC6F12.12 |par2|pbp2|protein phosphatase regulatory subunit Pa... 27 1.3
SPAC30D11.14c |||RNA-binding protein |Schizosaccharomyces pombe|... 27 1.3
SPCC550.03c |||RNA helicase involved in mRNA catabolism|Schizosa... 27 1.8
SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr 1... 27 2.3
SPBC365.11 |||GRIP domain protein|Schizosaccharomyces pombe|chr ... 26 4.1
SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family ... 25 5.4
SPBC1D7.02c |scr1||transcription factor Scr1|Schizosaccharomyces... 25 5.4
SPAC22F3.03c |rdh54|tid1, mug34|ATP-dependent DNA helicase Rdh54... 25 7.1
SPAC1B9.02c |sck1||serine/threonine protein kinase Sck1|Schizosa... 25 9.4
SPBC13A2.03 |||phosphatidate cytidylyltransferase|Schizosaccharo... 25 9.4
>SPBC21.05c |ral2||Ras guanyl-nucleotide exchange factor Ral2
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 611
Score = 29.9 bits (64), Expect = 0.25
Identities = 19/86 (22%), Positives = 39/86 (45%), Gaps = 2/86 (2%)
Frame = +2
Query: 278 VFLAGLIMCGGFFMMYLVEELVHLYIHARE--HKPSEKNDAKPFSLRKSISGNDPEINQE 451
+F G+ +Y++ ++ I ++ H P + + K ++G+D ++++
Sbjct: 98 IFFGGMGFNDDTKCLYVLNDIDIYDIETKQWSHIPGMITENQTNDDAKEVNGSD--VDEK 155
Query: 452 TKQIENETNSGHSHLVLDSSQEXFCG 529
+K + GH H VLD FCG
Sbjct: 156 SKHLYPSARYGHLHCVLDHYLIIFCG 181
>SPCC188.02 |par1||protein phosphatase regulatory subunit Par1
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 548
Score = 28.7 bits (61), Expect = 0.58
Identities = 11/54 (20%), Positives = 27/54 (50%)
Frame = -3
Query: 304 AHYQTGKEHWHIGDLSCLLQQFNVFVHLRQEMHERCSEKYSATKRQQSHHQLIR 143
A ++ K HW+ S + +F+ + + + +YS ++R++ ++IR
Sbjct: 452 ALFEISKSHWNRVIHSMVCNVLKLFMDINPSLFDEVDAEYSESRRKKEDEEIIR 505
>SPCC895.06 |||RNA polymerase II elongator complex subunit Elp2
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 760
Score = 28.7 bits (61), Expect = 0.58
Identities = 14/39 (35%), Positives = 19/39 (48%)
Frame = +3
Query: 168 WRLVAEYFSLQRSCISCLRWTKTLNCCKRQERSPMCQCS 284
W+L EYF+ CI + T+NC E +C CS
Sbjct: 83 WQLEEEYFT----CIKTIELEATVNCLCVNENLVVCGCS 117
>SPAC6F12.12 |par2|pbp2|protein phosphatase regulatory subunit
Par2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 627
Score = 27.5 bits (58), Expect = 1.3
Identities = 18/74 (24%), Positives = 30/74 (40%), Gaps = 5/74 (6%)
Frame = -3
Query: 298 YQTGKEHWHIGDLSCLLQQFNVFVHLRQEMHERCSEKYSATKRQQSHHQLIRRPQVW--- 128
Y+T EHW+ + +FV + + E Y +Q +IR+ Q W
Sbjct: 555 YKTANEHWNSTIQAIACNVLQIFVDMDADFFNGLVEDYKQAIIKQEEVMIIRKQQ-WCQI 613
Query: 127 --IAGFREPVELLR 92
+A +P + LR
Sbjct: 614 EALAAENKPTDYLR 627
>SPAC30D11.14c |||RNA-binding protein |Schizosaccharomyces pombe|chr
1|||Manual
Length = 534
Score = 27.5 bits (58), Expect = 1.3
Identities = 21/50 (42%), Positives = 24/50 (48%)
Frame = +1
Query: 295 DNVRRILHDVLSRRISSLVHTRQGAQTIREERRQTVQPKEEHLGERSRNK 444
D R D SRR S R G + RE R KEEHL ERSR++
Sbjct: 17 DRRSRFTEDSYSRRDSQ----RSGNEAPRESRYYR---KEEHLQERSRSR 59
>SPCC550.03c |||RNA helicase involved in mRNA
catabolism|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1213
Score = 27.1 bits (57), Expect = 1.8
Identities = 13/34 (38%), Positives = 21/34 (61%), Gaps = 1/34 (2%)
Frame = +2
Query: 206 MHLLPEVDENIELLQETGEI-PDVPVFLAGLIMC 304
+ LLP+ ++ I++LQE G I + V L G + C
Sbjct: 1014 LELLPDYEQRIKVLQELGYIDAERTVLLKGRVAC 1047
>SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 857
Score = 26.6 bits (56), Expect = 2.3
Identities = 17/61 (27%), Positives = 27/61 (44%)
Frame = -1
Query: 342 TNSSTKYIMKNPPHIIKPARNTGTSGISPVSCNNSMFSSTSGRRCMNVVAKSTPPPNANS 163
++ K I P KP+ +T T+G S S +S +T + + K P P + S
Sbjct: 258 SHGPAKQISVQPSEHPKPSISTTTTGSSYRSAESSHAPTTPDHFKLTPLTKLEPQPPSGS 317
Query: 162 P 160
P
Sbjct: 318 P 318
>SPBC365.11 |||GRIP domain protein|Schizosaccharomyces pombe|chr
2|||Manual
Length = 266
Score = 25.8 bits (54), Expect = 4.1
Identities = 19/73 (26%), Positives = 33/73 (45%)
Frame = +1
Query: 241 IVARDRRDPRCASVPCRFDNVRRILHDVLSRRISSLVHTRQGAQTIREERRQTVQPKEEH 420
I ++D+ P V +N + L D++ RRI + + Q R + K+EH
Sbjct: 70 IDSKDKEKPLDEKVK-ELENANKTLSDLV-RRIQ--IQRDEAEQKAEIYNRDALNTKQEH 125
Query: 421 LGERSRNKPRDQT 459
L + R + D+T
Sbjct: 126 LDIKKRLEKSDET 138
>SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1420
Score = 25.4 bits (53), Expect = 5.4
Identities = 16/57 (28%), Positives = 31/57 (54%), Gaps = 4/57 (7%)
Frame = +2
Query: 356 HAREHKPSEKNDAKPFSL----RKSISGNDPEINQETKQIENETNSGHSHLVLDSSQ 514
H +HK K++A+P S R+S S + +++ K+ +++ + HS DSS+
Sbjct: 593 HESKHKRDSKHEARPESKHESHRESKSAEKDKKDKKDKKEDSKRSRSHSVSKPDSSK 649
>SPBC1D7.02c |scr1||transcription factor Scr1|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 565
Score = 25.4 bits (53), Expect = 5.4
Identities = 19/63 (30%), Positives = 30/63 (47%), Gaps = 1/63 (1%)
Frame = -1
Query: 336 SSTKYIMKNPPHIIKPARNTGTSGISPVSCNNSM-FSSTSGRRCMNVVAKSTPPPNANSP 160
SS + + PH KPA + + S S FSS+S N++A S P P++++
Sbjct: 484 SSLSNVNSSMPH--KPASQSNVGPVRISSNRRSRKFSSSSRVSVSNLLAGSPPSPSSSTK 541
Query: 159 ITS 151
S
Sbjct: 542 SAS 544
>SPAC22F3.03c |rdh54|tid1, mug34|ATP-dependent DNA helicase
Rdh54|Schizosaccharomyces pombe|chr 1|||Manual
Length = 811
Score = 25.0 bits (52), Expect = 7.1
Identities = 12/32 (37%), Positives = 19/32 (59%), Gaps = 3/32 (9%)
Frame = -1
Query: 462 ICLVSWFISGSFPEMLFLRLN---GLASFFSD 376
+C+ ++ SG E +F+R N GL+S F D
Sbjct: 652 VCIYTFLSSGMLDERIFIRQNTKQGLSSSFID 683
>SPAC1B9.02c |sck1||serine/threonine protein kinase
Sck1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 696
Score = 24.6 bits (51), Expect = 9.4
Identities = 16/50 (32%), Positives = 32/50 (64%)
Frame = -1
Query: 255 VSCNNSMFSSTSGRRCMNVVAKSTPPPNANSPITS*FEDRRFGSRDFVNQ 106
VS + F++T+ + +N+V+ + P NA++P+++ +D RF FVN+
Sbjct: 590 VSNFDKEFTNTNVKN-INIVS-NVDPANASTPLSNTIQD-RFRGFTFVNK 636
>SPBC13A2.03 |||phosphatidate
cytidylyltransferase|Schizosaccharomyces pombe|chr
2|||Manual
Length = 439
Score = 24.6 bits (51), Expect = 9.4
Identities = 12/36 (33%), Positives = 20/36 (55%), Gaps = 1/36 (2%)
Frame = -1
Query: 336 SSTKYIMKNPPHIIKPARNTGTSGISPVSCN-NSMF 232
S Y++ + + I P R+ TS S ++C NS+F
Sbjct: 258 SLISYVLMHFKYFICPTRDLSTSAFSGLNCTPNSVF 293
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,361,053
Number of Sequences: 5004
Number of extensions: 52400
Number of successful extensions: 221
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 211
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 220
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 220420454
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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