Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmmt25a23
(669 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC6B12.12 |tom70||mitochondrial TOM complex subunit Tom70|Schi... 78 1e-15
SPAC1142.02c ||SPAC17G6.19c|TPR repeat protein|Schizosaccharomyc... 62 7e-11
SPBC543.02c |||DNAJ/TPR domain protein DNAJC7 family|Schizosacch... 54 2e-08
SPAC17A2.04c |||HSP chaperone complex subunit |Schizosaccharomyc... 47 2e-06
SPCC645.14c |sti1||chaperone activator Sti1 |Schizosaccharomyces... 45 1e-05
SPBC3F6.01c |||serine/threonine protein phosphatase |Schizosacch... 44 3e-05
SPAC2F3.02 |||ER protein translocation subcomplex subunit|Schizo... 34 0.016
SPBC17A3.05c |||DNAJ/DUF1977 DNAJB12 homolog|Schizosaccharomyces... 31 0.11
SPBC32H8.06 |mug93||TPR repeat protein, meiotically spliced|Schi... 31 0.15
SPCC338.16 |pof3||F-box protein Pof3|Schizosaccharomyces pombe|c... 30 0.35
SPCC16C4.14c |sfc4||transcription factor TFIIIC complex subunit ... 27 1.8
SPAC23C4.10 |sec2||guanyl-nucleotide exchange factor Sec2 |Schiz... 26 5.6
SPCC63.10c |||dolichol kinase |Schizosaccharomyces pombe|chr 3||... 26 5.6
SPAC17G6.17 |pof8||F-box protein Pof8|Schizosaccharomyces pombe|... 26 5.6
SPAC16A10.03c |||zinc finger protein Pep5/Vps11 |Schizosaccharom... 26 5.6
SPBC28F2.07 |sfr1|dds20, mug13|Swi five-dependent recombination ... 25 7.5
SPAC3H8.09c |nab3||poly|Schizosaccharomyces pombe|chr 1|||Manual 25 7.5
SPBC2F12.13 |klp5|sot1|kinesin-like protein Klp5|Schizosaccharom... 25 7.5
SPBC31E1.04 |pep12||SNARE Pep12|Schizosaccharomyces pombe|chr 2|... 25 9.9
>SPAC6B12.12 |tom70||mitochondrial TOM complex subunit
Tom70|Schizosaccharomyces pombe|chr 1|||Manual
Length = 625
Score = 78.2 bits (184), Expect = 1e-15
Identities = 52/144 (36%), Positives = 79/144 (54%), Gaps = 1/144 (0%)
Frame = +1
Query: 241 RNRLEDPEKKKASELKAKTTISLDNEENTTKVS-ESAIERAMKLKGAGNRAFHAGEYDKA 417
+ + ++ +K KA ++ + + EE+ ++ E + A +LK GN+A+ EY A
Sbjct: 111 KKKKKNKKKVKAKKVGDSSVDKIATEESVKSMTKEERAKLAAELKTLGNKAYGQKEYANA 170
Query: 418 IALYNEAIEACPPDRPVDLATFYQNRSACYEKREMWEQVKEDCTFALKLNEKYVKAFLRR 597
I Y +AI C D P+ F+ NR+ACY +EQV +D + AL L+ YVKA RR
Sbjct: 171 IDYYTQAI-TCSHD-PI----FFSNRAACYAAIGDFEQVIKDTSEALSLDSSYVKALNRR 224
Query: 598 SRAAEKSGDLVLALEDVTSACILE 669
S A E+ G L AL D T +CI +
Sbjct: 225 SAAYEQLGKLDEALMDSTVSCIFD 248
>SPAC1142.02c ||SPAC17G6.19c|TPR repeat protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 317
Score = 62.1 bits (144), Expect = 7e-11
Identities = 41/109 (37%), Positives = 56/109 (51%)
Frame = +1
Query: 316 EENTTKVSESAIERAMKLKGAGNRAFHAGEYDKAIALYNEAIEACPPDRPVDLATFYQNR 495
E++T V++ E A KLK GN A A +Y KA+ LY +AIE P PV +Y NR
Sbjct: 72 EDSTAHVNK---EEAEKLKLEGNNAIAAKDYQKALDLYTKAIEI-DPTSPV----YYSNR 123
Query: 496 SACYEKREMWEQVKEDCTFALKLNEKYVKAFLRRSRAAEKSGDLVLALE 642
+A Y + +E ED L L+ + +AF R RA GD A +
Sbjct: 124 AAAYNQLGQFENAVEDALTCLSLDPHHARAFGRLGRAKLSLGDAAAAAD 172
>SPBC543.02c |||DNAJ/TPR domain protein DNAJC7
family|Schizosaccharomyces pombe|chr 2|||Manual
Length = 476
Score = 54.0 bits (124), Expect = 2e-08
Identities = 35/123 (28%), Positives = 54/123 (43%)
Frame = +1
Query: 301 ISLDNEENTTKVSESAIERAMKLKGAGNRAFHAGEYDKAIALYNEAIEACPPDRPVDLAT 480
+ LD + T K + + K GN F G Y A Y+EA++ PD +A
Sbjct: 204 LKLDPDCTTAKTLFKQVRKLENTKNQGNDLFRQGNYQDAYEKYSEALQI-DPDNKETVAK 262
Query: 481 FYQNRSACYEKREMWEQVKEDCTFALKLNEKYVKAFLRRSRAAEKSGDLVLALEDVTSAC 660
Y NR+ + + E+ D AL ++ Y+K R++A E A+ DV SA
Sbjct: 263 LYMNRATVLLRLKRPEEALSDSDNALAIDSSYLKGLKVRAKAHEALEKWEEAVRDVQSAI 322
Query: 661 ILE 669
L+
Sbjct: 323 ELD 325
>SPAC17A2.04c |||HSP chaperone complex subunit |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 358
Score = 47.2 bits (107), Expect = 2e-06
Identities = 26/85 (30%), Positives = 42/85 (49%)
Frame = +1
Query: 352 ERAMKLKGAGNRAFHAGEYDKAIALYNEAIEACPPDRPVDLATFYQNRSACYEKREMWEQ 531
E A + GN F + Y A Y +A+ D+ +++A Y NR+AC E + Q
Sbjct: 62 EVAQNFREHGNECFASKRYKDAEEFYTKALAQKCGDKDIEIAC-YSNRAACNLLFENYRQ 120
Query: 532 VKEDCTFALKLNEKYVKAFLRRSRA 606
V DC L+ + + KA+ R ++A
Sbjct: 121 VLNDCAQVLQRDSTHAKAYYRSAKA 145
>SPCC645.14c |sti1||chaperone activator Sti1 |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 591
Score = 44.8 bits (101), Expect = 1e-05
Identities = 28/90 (31%), Positives = 40/90 (44%)
Frame = +1
Query: 358 AMKLKGAGNRAFHAGEYDKAIALYNEAIEACPPDRPVDLATFYQNRSACYEKREMWEQVK 537
A +LK GN AF +Y AI + +AI + Y NRSACY + +
Sbjct: 2 AEELKAKGNAAFSKKDYKTAIDYFTQAIGLDERNH-----ILYSNRSACYASEKDYADAL 56
Query: 538 EDCTFALKLNEKYVKAFLRRSRAAEKSGDL 627
+D T +L + K + R+ A GDL
Sbjct: 57 KDATKCTELKPDWAKGWSRKGAALHGLGDL 86
Score = 42.7 bits (96), Expect = 5e-05
Identities = 24/85 (28%), Positives = 44/85 (51%)
Frame = +1
Query: 352 ERAMKLKGAGNRAFHAGEYDKAIALYNEAIEACPPDRPVDLATFYQNRSACYEKREMWEQ 531
++A + + GN F +G++ AI Y E + P D + NR+A Y K +
Sbjct: 397 DKAEESRVKGNELFKSGDFANAIKEYTEMTKRAPSD-----PRGFGNRAAAYLKVMAPAE 451
Query: 532 VKEDCTFALKLNEKYVKAFLRRSRA 606
DC A++L+ + KA++R+++A
Sbjct: 452 CIRDCNKAIELDPNFAKAYVRKAQA 476
>SPBC3F6.01c |||serine/threonine protein phosphatase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 473
Score = 43.6 bits (98), Expect = 3e-05
Identities = 27/85 (31%), Positives = 43/85 (50%)
Frame = +1
Query: 352 ERAMKLKGAGNRAFHAGEYDKAIALYNEAIEACPPDRPVDLATFYQNRSACYEKREMWEQ 531
+ A++LK N+ G +AI LY +AIE + A Y NRS + K E +
Sbjct: 3 KEALELKNEANKFLKEGHIVQAIDLYTKAIELDSTN-----AILYSNRSLAHLKSEDYGL 57
Query: 532 VKEDCTFALKLNEKYVKAFLRRSRA 606
D + A++ + +Y KA+ RR+ A
Sbjct: 58 AINDASKAIECDPEYAKAYFRRATA 82
>SPAC2F3.02 |||ER protein translocation subcomplex
subunit|Schizosaccharomyces pombe|chr 1|||Manual
Length = 192
Score = 34.3 bits (75), Expect = 0.016
Identities = 27/109 (24%), Positives = 47/109 (43%), Gaps = 8/109 (7%)
Frame = +1
Query: 364 KLKGAGNRAFHAGEYDKAIALYNEAIEA----CPPDRPV----DLATFYQNRSACYEKRE 519
K+K N AF +Y++A LY A++ C + + + + NR+A
Sbjct: 64 KMKQTANEAFKRKKYEEAKKLYGLALQLALNRCTWEPSILTREEASVMLCNRAAAEIALS 123
Query: 520 MWEQVKEDCTFALKLNEKYVKAFLRRSRAAEKSGDLVLALEDVTSACIL 666
+ + D ALK+ Y K + R+++A E + A + V IL
Sbjct: 124 QFPEALADANAALKIRNNYGKCYYRKAKALEAMHRIEEAKQVVRDGLIL 172
>SPBC17A3.05c |||DNAJ/DUF1977 DNAJB12 homolog|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 403
Score = 31.5 bits (68), Expect = 0.11
Identities = 13/34 (38%), Positives = 22/34 (64%)
Frame = +1
Query: 340 ESAIERAMKLKGAGNRAFHAGEYDKAIALYNEAI 441
ES+ E A++ G + +AGEYDKA+ N+++
Sbjct: 2 ESSREEALRCIGLARKYLNAGEYDKALKFANKSL 35
>SPBC32H8.06 |mug93||TPR repeat protein, meiotically
spliced|Schizosaccharomyces pombe|chr 2|||Manual
Length = 383
Score = 31.1 bits (67), Expect = 0.15
Identities = 21/81 (25%), Positives = 38/81 (46%)
Frame = +1
Query: 403 EYDKAIALYNEAIEACPPDRPVDLATFYQNRSACYEKREMWEQVKEDCTFALKLNEKYVK 582
E + I +++E + A + + Y + S + +++ + +V DCT+AL +N K
Sbjct: 19 ENTREIDVFDEFLNAIGNENTI--TPVYADSSLTHLRKKSYTKVVHDCTYALVINPYDKK 76
Query: 583 AFLRRSRAAEKSGDLVLALED 645
RR A + G LA D
Sbjct: 77 VIWRRGLAYLRLGHPHLANRD 97
>SPCC338.16 |pof3||F-box protein Pof3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 577
Score = 29.9 bits (64), Expect = 0.35
Identities = 17/73 (23%), Positives = 35/73 (47%)
Frame = +1
Query: 403 EYDKAIALYNEAIEACPPDRPVDLATFYQNRSACYEKREMWEQVKEDCTFALKLNEKYVK 582
+++ A+ + IE P+ +DL ++ R+ YEK + Q + D + LN + +
Sbjct: 21 KFEDALTFITKTIEQ-EPNPTIDL---FELRAQVYEKSGQYSQAELDAKRMIHLNARNAR 76
Query: 583 AFLRRSRAAEKSG 621
+LR + + G
Sbjct: 77 GYLRLGKLLQLDG 89
>SPCC16C4.14c |sfc4||transcription factor TFIIIC complex subunit
Sfc4|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1006
Score = 27.5 bits (58), Expect = 1.8
Identities = 15/52 (28%), Positives = 27/52 (51%)
Frame = +1
Query: 406 YDKAIALYNEAIEACPPDRPVDLATFYQNRSACYEKREMWEQVKEDCTFALK 561
+D+A YN A+ A PP++ +L + NRS ++ ++ E F L+
Sbjct: 221 WDQADYCYNRAVSAKPPNKS-ELKKYIWNRSVLNKEHGSLKKAAEGFKFLLQ 271
>SPAC23C4.10 |sec2||guanyl-nucleotide exchange factor Sec2
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 527
Score = 25.8 bits (54), Expect = 5.6
Identities = 13/53 (24%), Positives = 22/53 (41%)
Frame = +3
Query: 510 KTRNVGTS*RRLHFCIKT*RKVCKSISASISCCGKKWRSCACS*RCNICMYPR 668
++R TS +C+ R VC ++ C W SC+ + C+ R
Sbjct: 459 RSRPDSTSHASCTYCVARLRSVCNFVAFLHQICKGVWSSCSLEKAWDECLKKR 511
>SPCC63.10c |||dolichol kinase |Schizosaccharomyces pombe|chr
3|||Manual
Length = 465
Score = 25.8 bits (54), Expect = 5.6
Identities = 15/47 (31%), Positives = 23/47 (48%)
Frame = -1
Query: 609 RSTRSTQKCFYILFVKF*CKSAIFFNLFPHFSFFITSRAVLVKCSKI 469
R+ S + F +LF+ F C S F + SFF+ + L+ S I
Sbjct: 26 RNFESILEIFSVLFIPFLCNSGKKFLQISNASFFLPACFYLLGSSSI 72
>SPAC17G6.17 |pof8||F-box protein Pof8|Schizosaccharomyces pombe|chr
1|||Manual
Length = 402
Score = 25.8 bits (54), Expect = 5.6
Identities = 11/30 (36%), Positives = 18/30 (60%)
Frame = +2
Query: 428 TMKQLKHAPQTVLSILLHFTKTALLVMKNE 517
T LK+A V + L HF ++ +V++NE
Sbjct: 111 TQNNLKYALSYVTNALFHFDNSSHMVIRNE 140
>SPAC16A10.03c |||zinc finger protein Pep5/Vps11
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 860
Score = 25.8 bits (54), Expect = 5.6
Identities = 12/42 (28%), Positives = 25/42 (59%)
Frame = -3
Query: 661 YMQMLHLQEQAQDRHFFPQHEIDAEMLLHTFR*VLMQKCNLL 536
Y + L+ Q + +H PQ+E+DA ++H + +L + ++L
Sbjct: 601 YTRKLYALMQKELQHSNPQNELDALQIIHDSQGLLDYESSIL 642
>SPBC28F2.07 |sfr1|dds20, mug13|Swi five-dependent recombination
repair protein Sfr1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 299
Score = 25.4 bits (53), Expect = 7.5
Identities = 14/61 (22%), Positives = 27/61 (44%)
Frame = +1
Query: 277 SELKAKTTISLDNEENTTKVSESAIERAMKLKGAGNRAFHAGEYDKAIALYNEAIEACPP 456
S+ K +SL + + IE K +GN + + G +++ ++ E +A P
Sbjct: 15 SQCKEDLKVSLSESDLRDSQGQLGIENPPKCNNSGNHSDNLGFIEQSETVHPENEKALTP 74
Query: 457 D 459
D
Sbjct: 75 D 75
>SPAC3H8.09c |nab3||poly|Schizosaccharomyces pombe|chr 1|||Manual
Length = 738
Score = 25.4 bits (53), Expect = 7.5
Identities = 14/39 (35%), Positives = 19/39 (48%)
Frame = -2
Query: 647 TSSRASTRSPLFSAARDRRRNAFTYFSLSFNAKVQSSLT 531
TSSR TRSPL S + R S +F+ + +T
Sbjct: 468 TSSRKRTRSPLMSKGKSYDRKGSISMSKNFSPDCEILVT 506
>SPBC2F12.13 |klp5|sot1|kinesin-like protein
Klp5|Schizosaccharomyces pombe|chr 2|||Manual
Length = 883
Score = 25.4 bits (53), Expect = 7.5
Identities = 12/34 (35%), Positives = 19/34 (55%)
Frame = -3
Query: 454 GGMLQLLHCIKQWLCHILQHEKPGYQHLLVSWRV 353
GGM +L +++WL I EKP L S+++
Sbjct: 609 GGMQELFSILEKWLLGIGLGEKPNISVLSESYKL 642
>SPBC31E1.04 |pep12||SNARE Pep12|Schizosaccharomyces pombe|chr
2|||Manual
Length = 317
Score = 25.0 bits (52), Expect = 9.9
Identities = 14/33 (42%), Positives = 19/33 (57%)
Frame = +2
Query: 80 LTKLFTNKHSI*CLLKPSDNLRIWLLPVVHHFR 178
LTK F N+ + L S + R+W LP VH F+
Sbjct: 272 LTKHFRNRVLV--SLYVSCSSRVWSLPYVHAFK 302
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,711,511
Number of Sequences: 5004
Number of extensions: 55693
Number of successful extensions: 200
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 188
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 197
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 305854096
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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