Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmmt23i08
(633 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC1259.15c |ubc11|ubcdp, ubcp4|ubiquitin conjugating enzyme E2... 84 1e-17
SPAC11E3.04c |ubc13|spu13|ubiquitin conjugating enzyme Ubc13|Sch... 83 3e-17
SPAC18B11.07c |rhp6|ubc2|Rad6 homolog, ubiquitin conjugating enz... 81 1e-16
SPBC119.02 |ubc4||ubiquitin conjugating enzyme Ubc4|Schizosaccha... 80 2e-16
SPBC1198.09 |ubc16||ubiquitin conjugating enzyme Ubc16|Schizosac... 75 1e-14
SPBP16F5.04 |ubc3|ubcp3|ubiquitin conjugating enzyme Ubc3|Schizo... 67 2e-12
SPBC1105.09 |ubc15||ubiquitin conjugating enzyme Ubc15|Schizosac... 65 9e-12
SPBC2D10.20 |ubc1||ubiquitin conjugating enzyme Ubc1|Schizosacch... 65 9e-12
SPAC1250.03 |ubc14||ubiquitin conjugating enzyme Ubc14|Schizosac... 64 2e-11
SPAC30D11.13 |hus5|ubc9|SUMO conjugating enzyme Hus5|Schizosacch... 62 9e-11
SPCC777.10c |ubc12||ubiquitin conjugating enzyme Ubc12|Schizosac... 56 4e-09
SPAC10F6.05c |ubc6||ubiquitin conjugating enzyme Ubc6|Schizosacc... 52 5e-08
SPBC211.07c |ubc8||ubiquitin conjugating enzyme Ubc8|Schizosacch... 45 8e-06
SPAC30C2.07 |||sequence orphan|Schizosaccharomyces pombe|chr 1||... 26 3.9
SPBC12C2.03c |||FAD binding protein |Schizosaccharomyces pombe|c... 25 6.9
SPBC28E12.03 |rga4||GTPase activating protein Rga4|Schizosacchar... 25 6.9
SPCC757.08 |||exosome subunit Rrp45 |Schizosaccharomyces pombe|c... 25 9.1
>SPCC1259.15c |ubc11|ubcdp, ubcp4|ubiquitin conjugating enzyme E2-C
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 176
Score = 84.2 bits (199), Expect = 1e-17
Identities = 46/112 (41%), Positives = 63/112 (56%), Gaps = 1/112 (0%)
Frame = +3
Query: 297 RRLQKELMSLMKEPPPGVTVDGDQASQNLTLWTVHMEGVPGTLYEGEKFVLQFKFTNKYP 476
+RL+ ELMSLM PG++ D S NL W + G T YEG KF + F YP
Sbjct: 31 KRLRSELMSLMMSNTPGISAFPDSDS-NLLHWAGTITGPSDTYYEGLKFKISMSFPANYP 89
Query: 477 FDSPEVTFIGNNIPV-HPHVYSNGHICLSILTDDWSPALSVQSVCLSIVSML 629
+ P + F P+ HP+V +G+ICL IL D WS +VQ++ LS+ S+L
Sbjct: 90 YSPPTIIFTS---PMWHPNVDMSGNICLDILKDKWSAVYNVQTILLSLQSLL 138
>SPAC11E3.04c |ubc13|spu13|ubiquitin conjugating enzyme
Ubc13|Schizosaccharomyces pombe|chr 1|||Manual
Length = 148
Score = 83.0 bits (196), Expect = 3e-17
Identities = 43/111 (38%), Positives = 65/111 (58%)
Frame = +3
Query: 297 RRLQKELMSLMKEPPPGVTVDGDQASQNLTLWTVHMEGVPGTLYEGEKFVLQFKFTNKYP 476
+R+ KE+ +L ++PPPG+ + NL + + MEG + YEG KF L+ ++YP
Sbjct: 5 KRIIKEIETLTRDPPPGIVAAPTE--DNLRYFKITMEGPQQSAYEGGKFHLELFLPDEYP 62
Query: 477 FDSPEVTFIGNNIPVHPHVYSNGHICLSILTDDWSPALSVQSVCLSIVSML 629
P V F+ HP+V G ICLS L DWSPAL +++V LSI +++
Sbjct: 63 MMPPNVRFLTKIY--HPNVDKLGRICLSTLKKDWSPALQIRTVLLSIQALM 111
>SPAC18B11.07c |rhp6|ubc2|Rad6 homolog, ubiquitin conjugating enzyme
E2 Rhp6|Schizosaccharomyces pombe|chr 1|||Manual
Length = 151
Score = 81.0 bits (191), Expect = 1e-16
Identities = 41/117 (35%), Positives = 62/117 (52%)
Frame = +3
Query: 282 SVNSQRRLQKELMSLMKEPPPGVTVDGDQASQNLTLWTVHMEGVPGTLYEGEKFVLQFKF 461
S ++RRL ++ + ++PP GV+ S N+ LW + G T +E F L F
Sbjct: 2 STTARRRLMRDFKRMQQDPPAGVSAS--PVSDNVMLWNAVIIGPADTPFEDGTFKLVLSF 59
Query: 462 TNKYPFDSPEVTFIGNNIPVHPHVYSNGHICLSILTDDWSPALSVQSVCLSIVSMLS 632
+YP P V F+ HP+VY+NG +CL IL + WSP V ++ SI S+L+
Sbjct: 60 DEQYPNKPPLVKFVSTMF--HPNVYANGELCLDILQNRWSPTYDVAAILTSIQSLLN 114
>SPBC119.02 |ubc4||ubiquitin conjugating enzyme
Ubc4|Schizosaccharomyces pombe|chr 2|||Manual
Length = 147
Score = 80.2 bits (189), Expect = 2e-16
Identities = 43/112 (38%), Positives = 58/112 (51%)
Frame = +3
Query: 297 RRLQKELMSLMKEPPPGVTVDGDQASQNLTLWTVHMEGVPGTLYEGEKFVLQFKFTNKYP 476
+R+ +EL L K+PP + +L W + G + Y G F L F YP
Sbjct: 4 KRINRELADLGKDPPSSCSAG--PVGDDLFHWQATIMGPADSPYAGGVFFLSIHFPTDYP 61
Query: 477 FDSPEVTFIGNNIPVHPHVYSNGHICLSILTDDWSPALSVQSVCLSIVSMLS 632
F P+V F HP++ SNG ICL IL D WSPAL++ V LSI S+L+
Sbjct: 62 FKPPKVNFTTRIY--HPNINSNGSICLDILRDQWSPALTISKVLLSICSLLT 111
>SPBC1198.09 |ubc16||ubiquitin conjugating enzyme
Ubc16|Schizosaccharomyces pombe|chr 2|||Manual
Length = 160
Score = 74.5 bits (175), Expect = 1e-14
Identities = 42/116 (36%), Positives = 61/116 (52%), Gaps = 1/116 (0%)
Frame = +3
Query: 288 NSQRRLQKELMSLMKEPPPGVTVDGDQASQNLTLWTVHMEGVPGTLYEGEKFVLQFKFTN 467
+S +RL KE S G+ + ++ W +EG T YEG ++VL
Sbjct: 3 SSIKRLHKEYASFQAGDISGLLLLKPVTDVDMFHWKAVIEGPTETPYEGGQWVLDIHVHE 62
Query: 468 KYPFDSPEVTFIGNNIPVHPHV-YSNGHICLSILTDDWSPALSVQSVCLSIVSMLS 632
YP P V F VHP++ ++NG +C+ IL WSPA S+QS CL+I+S+LS
Sbjct: 63 GYPISPPSVYF--QTKIVHPNISWTNGEVCMDILKTHWSPAWSLQSACLAIISLLS 116
>SPBP16F5.04 |ubc3|ubcp3|ubiquitin conjugating enzyme
Ubc3|Schizosaccharomyces pombe|chr 2|||Manual
Length = 166
Score = 67.3 bits (157), Expect = 2e-12
Identities = 41/125 (32%), Positives = 61/125 (48%), Gaps = 13/125 (10%)
Frame = +3
Query: 297 RRLQKELMSLMKEPPPGVTVDGDQASQNLTLWTVHMEGVPGTLYEGEKFVLQFKFTNKYP 476
RRL KE L + P G+T G + W ++G GT +EG + KF + YP
Sbjct: 8 RRLMKEYKELTENGPDGITA-GPSNEDDFFTWDCLIQGPDGTPFEGGLYPATLKFPSDYP 66
Query: 477 FDSPEVTFIGNNIPVHPHVYSNGHICLSIL-------------TDDWSPALSVQSVCLSI 617
P + F HP+VY +G +C+SIL ++ WSP SV+ + LS+
Sbjct: 67 LGPPTLKFECEFF--HPNVYKDGTVCISILHAPGDDPNMYESSSERWSPVQSVEKILLSV 124
Query: 618 VSMLS 632
+SML+
Sbjct: 125 MSMLA 129
>SPBC1105.09 |ubc15||ubiquitin conjugating enzyme
Ubc15|Schizosaccharomyces pombe|chr 2|||Manual
Length = 167
Score = 64.9 bits (151), Expect = 9e-12
Identities = 43/127 (33%), Positives = 65/127 (51%), Gaps = 13/127 (10%)
Frame = +3
Query: 291 SQRRLQKELMSLMKEPPPGVTVDGDQASQNLTLWTVHMEGVPGTLYEGEKFVLQFKFTNK 470
S++ L+K+L + K PP G +V G +++ W V + G TLYEG F F
Sbjct: 6 SEQLLRKQLKEIQKNPPQGFSV-GLVDDKSIFEWEVMIIGPEDTLYEGGFFHATLSFPQD 64
Query: 471 YPFDSPEVTFIGNNIPVHPHVYSNGHICLSIL---TDD----------WSPALSVQSVCL 611
YP P++ F HP+V+ NG +C+SIL DD W P S +++ +
Sbjct: 65 YPLMPPKMKF--TTEIWHPNVHPNGEVCISILHPPGDDKYGYEDAGERWLPVHSPETILI 122
Query: 612 SIVSMLS 632
S++SMLS
Sbjct: 123 SVISMLS 129
>SPBC2D10.20 |ubc1||ubiquitin conjugating enzyme
Ubc1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 217
Score = 64.9 bits (151), Expect = 9e-12
Identities = 40/117 (34%), Positives = 61/117 (52%), Gaps = 1/117 (0%)
Frame = +3
Query: 282 SVNSQRRLQKELMSLMKEPPPGVTVDGDQASQNLTLWTVHMEGVPGTLYEGEKFVLQFKF 461
S N RR+ KEL + ++ G+ V + +++ G GT YEG FV+ +
Sbjct: 2 SDNRSRRIAKELADVQQDKQAGIQVW--TINDDISHLKGMFRGPEGTPYEGGYFVVDIEI 59
Query: 462 TNKYPFDSPEVTFIGNNIPVHPHVYSN-GHICLSILTDDWSPALSVQSVCLSIVSML 629
YPF P++ F + HP+V S G ICL IL D WSP +++S +S+ S+L
Sbjct: 60 PIDYPFRPPKMNF--DTKIYHPNVSSQTGAICLDILKDQWSPVYTMKSALISLQSLL 114
>SPAC1250.03 |ubc14||ubiquitin conjugating enzyme
Ubc14|Schizosaccharomyces pombe|chr 1|||Manual
Length = 155
Score = 64.1 bits (149), Expect = 2e-11
Identities = 40/121 (33%), Positives = 58/121 (47%), Gaps = 1/121 (0%)
Frame = +3
Query: 270 MYYTSVNSQRRLQKELMSLMKEPPPGVTVDGDQASQNLTLWTVHMEGVPGTLYEGEKFVL 449
M S +S RRL KE L + P P + V+ NL W G ++Y G KF
Sbjct: 1 MASASPSSSRRLTKEYSDLREHPIPDIRVN--LVDDNLFHWACTALGPSDSVYAGGKFHF 58
Query: 450 QFKFTNKYPFDSPEVTFIGNNIPVHPHVYSNGHICLSILTDD-WSPALSVQSVCLSIVSM 626
KF YPF P + F HP+ S G++CL+IL + P++ ++SV I+ +
Sbjct: 59 SLKFPLDYPFQPPTIEF--TTRIYHPNFDSEGNVCLAILKQQVFKPSIKLRSVLEQILQL 116
Query: 627 L 629
L
Sbjct: 117 L 117
>SPAC30D11.13 |hus5|ubc9|SUMO conjugating enzyme
Hus5|Schizosaccharomyces pombe|chr 1|||Manual
Length = 157
Score = 61.7 bits (143), Expect = 9e-11
Identities = 37/115 (32%), Positives = 59/115 (51%), Gaps = 5/115 (4%)
Frame = +3
Query: 300 RLQKELMSLMKEPPPGVTVDGDQASQ---NLTLWTVHMEGVPGTLYEGEKFVLQFKFTNK 470
RLQ+E ++ P G ++S +L W V + G P T +EG + L F +
Sbjct: 8 RLQEERKQWRRDHPFGFYAKPCKSSDGGLDLMNWKVGIPGKPKTSWEGGLYKLTMAFPEE 67
Query: 471 YPFDSPEVTFIGNNIPVHPHVYSNGHICLSILTDD--WSPALSVQSVCLSIVSML 629
YP P+ F HP+VY +G +CLSIL ++ W PA++++ + L I +L
Sbjct: 68 YPTRPPKCRFTPPLF--HPNVYPSGTVCLSILNEEEGWKPAITIKQILLGIQDLL 120
>SPCC777.10c |ubc12||ubiquitin conjugating enzyme
Ubc12|Schizosaccharomyces pombe|chr 3|||Manual
Length = 177
Score = 56.0 bits (129), Expect = 4e-09
Identities = 34/110 (30%), Positives = 60/110 (54%), Gaps = 1/110 (0%)
Frame = +3
Query: 291 SQRRLQKELMSLMKEPPPGVTVDGDQASQNLTLWTVHMEGVPGT-LYEGEKFVLQFKFTN 467
+Q R+QK++ L E P ++ + L +H+E P Y+G KF + + +
Sbjct: 24 AQIRIQKDVTDL--EIPSTMSTSWPDP---IKLNVLHLEIRPDEGYYKGGKFKFRIQIDD 78
Query: 468 KYPFDSPEVTFIGNNIPVHPHVYSNGHICLSILTDDWSPALSVQSVCLSI 617
YP D P+V + N I HP++ G++CL+IL DW+P L++ S+ + +
Sbjct: 79 NYPHDPPKVKCL-NKI-YHPNIDIEGNVCLNILRQDWNPVLNLNSILVGL 126
>SPAC10F6.05c |ubc6||ubiquitin conjugating enzyme
Ubc6|Schizosaccharomyces pombe|chr 1|||Manual
Length = 227
Score = 52.4 bits (120), Expect = 5e-08
Identities = 34/120 (28%), Positives = 56/120 (46%), Gaps = 3/120 (2%)
Frame = +3
Query: 282 SVNSQRRLQKELMSLMKEPPPGVTVDGDQASQNLTLWTVHMEGVPGTLYEGEKFVLQFKF 461
S + +RL KE ++L K P VD A++N+ W + G P T YEG ++ F
Sbjct: 3 SKGAYKRLMKEYLALQKNPVE--LVDAKPATENILEWHYIITGPPDTPYEGGQYHGTLIF 60
Query: 462 TNKYPFDSPEVTFIGNNIPVHPHVYSNGHICLSIL---TDDWSPALSVQSVCLSIVSMLS 632
YPF P + I + +N +CLS W+P+ V ++ + +VS ++
Sbjct: 61 PPDYPFKPPAIRMITPS----GRFQTNTRLCLSFSDFHPKSWNPSWMVSTILVGLVSFMT 116
>SPBC211.07c |ubc8||ubiquitin conjugating enzyme
Ubc8|Schizosaccharomyces pombe|chr 2|||Manual
Length = 184
Score = 45.2 bits (102), Expect = 8e-06
Identities = 28/105 (26%), Positives = 54/105 (51%), Gaps = 1/105 (0%)
Frame = +3
Query: 294 QRRLQKELMSLMKEPPPGVTVDGDQASQNLTLWTVHMEGVPGTLYEGEKFVLQFKFTNKY 473
+RR++ ++M L+ VT+ D N+ + V G T Y G + + + ++Y
Sbjct: 5 RRRIETDVMKLLMSDYE-VTLVND----NMQEFYVRFHGPSETPYSGGIWKVHVELPSEY 59
Query: 474 PFDSPEVTFIGNNIPVHPHVYS-NGHICLSILTDDWSPALSVQSV 605
P+ SP + F+ N I HP++ +G +CL ++ WSP + ++
Sbjct: 60 PWKSPSIGFV-NRI-FHPNIDELSGSVCLDVINQTWSPMFDMINI 102
>SPAC30C2.07 |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 842
Score = 26.2 bits (55), Expect = 3.9
Identities = 15/50 (30%), Positives = 24/50 (48%), Gaps = 1/50 (2%)
Frame = +3
Query: 171 NIFGDQNVLL*TISFKMISLINYEAGRCYKPVNMYYTSV-NSQRRLQKEL 317
++F D + + +I + YE PV+ YYT + N LQKE+
Sbjct: 317 SLFSDTRTRPASYALALIITVPYEYDEIVHPVSTYYTMLSNFTLSLQKEI 366
>SPBC12C2.03c |||FAD binding protein |Schizosaccharomyces pombe|chr
2|||Manual
Length = 571
Score = 25.4 bits (53), Expect = 6.9
Identities = 9/23 (39%), Positives = 15/23 (65%)
Frame = +1
Query: 562 SSPTTGRRRCRCNQSASRSCRCS 630
SSP++ + C N++ +CRCS
Sbjct: 20 SSPSSNFKTCVSNENGCINCRCS 42
>SPBC28E12.03 |rga4||GTPase activating protein
Rga4|Schizosaccharomyces pombe|chr 2|||Manual
Length = 933
Score = 25.4 bits (53), Expect = 6.9
Identities = 10/25 (40%), Positives = 15/25 (60%)
Frame = +3
Query: 453 FKFTNKYPFDSPEVTFIGNNIPVHP 527
F+ N PFDS + G++IP+ P
Sbjct: 251 FRSANASPFDSCDSFHSGSSIPIEP 275
>SPCC757.08 |||exosome subunit Rrp45 |Schizosaccharomyces pombe|chr
3|||Manual
Length = 291
Score = 25.0 bits (52), Expect = 9.1
Identities = 13/30 (43%), Positives = 17/30 (56%)
Frame = +3
Query: 477 FDSPEVTFIGNNIPVHPHVYSNGHICLSIL 566
F PE+T +G + VHP V + LSIL
Sbjct: 161 FRRPELTVVGEEVTVHP-VEERVPVPLSIL 189
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,585,348
Number of Sequences: 5004
Number of extensions: 55380
Number of successful extensions: 159
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 133
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 138
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 281707720
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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