Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmmt23c09
(607 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC18G6.04c |shm2||serine hydroxymethyltransferase Shm2 |Schizo... 177 8e-46
SPAC24C9.12c |||glycine hydroxymethyltransferase |Schizosaccharo... 165 6e-42
SPAC167.01 |ppk4||serine/threonine protein kinase Ppk4 |Schizosa... 31 0.17
SPBC32H8.13c |mok12||alpha-1,3-glucan synthase Mok12|Schizosacch... 29 0.70
SPAC1F3.05 |||adaptin |Schizosaccharomyces pombe|chr 1|||Manual 28 1.2
SPAC4A8.12c |sds22||protein phosphatase regulatory subunit Sds22... 27 1.6
SPAC1F12.02c |p23fy||translationally controlled tumor protein ho... 27 2.1
SPAC30.01c |sec72|sec7b|Sec7 domain|Schizosaccharomyces pombe|ch... 26 3.7
SPAC869.08 |pcm2||protein-L-isoaspartate O-methyltransferase |Sc... 26 4.9
SPAC20H4.10 |ufd2||ubiquitin-protein ligase E4 |Schizosaccharomy... 25 6.5
SPAC3F10.17 |||ribosome biogenesis protein Ltv1|Schizosaccharomy... 25 6.5
SPBC20F10.08c |||conserved eukaryotic protein|Schizosaccharomyce... 25 8.6
SPAC29E6.03c |uso1|SPAC30.07c|ER to Golgi tethering factor Uso1 ... 25 8.6
SPAC2G11.02 |urb2||ribosome biogenesis protein Urb2 |Schizosacch... 25 8.6
>SPAC18G6.04c |shm2||serine hydroxymethyltransferase Shm2
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 472
Score = 177 bits (432), Expect = 8e-46
Identities = 79/136 (58%), Positives = 99/136 (72%)
Frame = +3
Query: 195 KLLNSNLWEADPELFDIIVKEKDRQRAGLEMIASENFTSVPVLQCLSSCLHNKYSEGMPN 374
KLL + L E DP ++ I+ EK RQ+ + +IASENFTS V+ L S + NKYSEG P
Sbjct: 12 KLLKAPLAECDPTVYKILESEKSRQKESIALIASENFTSRAVMDALGSIMQNKYSEGYPG 71
Query: 375 QRYYGGNEYIDEIEILAQNRSLEAYRLKSEEWGVNVQPYSGSPANFAVYTGIVEPHGRIM 554
RYYGGNE+ID+ E L Q R+LEA+ L E+WGVNVQP+SGSPAN Y +++PH R+M
Sbjct: 72 ARYYGGNEFIDQAERLCQTRALEAFHLDGEKWGVNVQPHSGSPANLQAYQAVMKPHDRLM 131
Query: 555 GLDLPDGGHLTHGFFT 602
GLDLP GGHL+HGF T
Sbjct: 132 GLDLPHGGHLSHGFST 147
>SPAC24C9.12c |||glycine hydroxymethyltransferase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 467
Score = 165 bits (400), Expect = 6e-42
Identities = 76/130 (58%), Positives = 94/130 (72%)
Frame = +3
Query: 213 LWEADPELFDIIVKEKDRQRAGLEMIASENFTSVPVLQCLSSCLHNKYSEGMPNQRYYGG 392
L E DP + +I+ E DRQR+ + +IASENFTS V+ L S + NKYSEG P RYYGG
Sbjct: 12 LKEQDPTVAEIMRHEADRQRSSVVLIASENFTSRAVMDALGSVMSNKYSEGYPGARYYGG 71
Query: 393 NEYIDEIEILAQNRSLEAYRLKSEEWGVNVQPYSGSPANFAVYTGIVEPHGRIMGLDLPD 572
N++ID+IE L Q R+L A+ L +WGVNVQ SGSPAN VY I+ PHGR+MGLDLP
Sbjct: 72 NKFIDQIETLCQERALAAFNLDPAKWGVNVQCLSGSPANMQVYQAIMPPHGRLMGLDLPS 131
Query: 573 GGHLTHGFFT 602
GGHL+HG+ T
Sbjct: 132 GGHLSHGYQT 141
>SPAC167.01 |ppk4||serine/threonine protein kinase Ppk4
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1072
Score = 30.7 bits (66), Expect = 0.17
Identities = 16/62 (25%), Positives = 33/62 (53%)
Frame = +3
Query: 249 VKEKDRQRAGLEMIASENFTSVPVLQCLSSCLHNKYSEGMPNQRYYGGNEYIDEIEILAQ 428
V+E+D L+M+ EN + + + ++CLH+ + + R Y G++ +D + +L
Sbjct: 957 VEERDPPSPLLQML--ENNSKSVIGENWTTCLHSSLVDNLGKYRKYDGSKILDILRVLRN 1014
Query: 429 NR 434
R
Sbjct: 1015 KR 1016
>SPBC32H8.13c |mok12||alpha-1,3-glucan synthase
Mok12|Schizosaccharomyces pombe|chr 2|||Manual
Length = 2352
Score = 28.7 bits (61), Expect = 0.70
Identities = 20/74 (27%), Positives = 37/74 (50%), Gaps = 3/74 (4%)
Frame = +3
Query: 324 QCLSSCLH-NKYSEGMPNQRYYG-GNEY-IDEIEILAQNRSLEAYRLKSEEWGVNVQPYS 494
+C+S + + ++ + +Q Y G N++ I E+ + N+S +A + +G PYS
Sbjct: 1762 ECVSKDIEVDNFALNLQSQSYEGDSNDFGIREVPLSDANQSSQADSTSIDRYG----PYS 1817
Query: 495 GSPANFAVYTGIVE 536
NF+ Y VE
Sbjct: 1818 SQKVNFSKYKDFVE 1831
>SPAC1F3.05 |||adaptin |Schizosaccharomyces pombe|chr 1|||Manual
Length = 510
Score = 27.9 bits (59), Expect = 1.2
Identities = 18/52 (34%), Positives = 28/52 (53%)
Frame = -1
Query: 292 AIISSPAR*RSFSLTIISKSSGSASQRLLFSNLALIFVELMYLFAANVLEFL 137
+I+ S + S LT + KSSG AS + FSN +L + +F V++ L
Sbjct: 397 SILQSDSLMISTQLTSVQKSSGFASYSVQFSNCSLTWPVSEVVFQVAVVKSL 448
>SPAC4A8.12c |sds22||protein phosphatase regulatory subunit Sds22
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 332
Score = 27.5 bits (58), Expect = 1.6
Identities = 18/54 (33%), Positives = 25/54 (46%), Gaps = 2/54 (3%)
Frame = +3
Query: 267 QRAGLEMIASENFTSVPVLQCLSSCLHNKY--SEGMPNQRYYGGNEYIDEIEIL 422
Q+ L I S T L CLS CL Y G+ + + G E ++ +EIL
Sbjct: 192 QKLSLLSIQSNRITQFENLACLSHCLRELYVSHNGLTS---FSGIEVLENLEIL 242
>SPAC1F12.02c |p23fy||translationally controlled tumor protein
homolog|Schizosaccharomyces pombe|chr 1|||Manual
Length = 168
Score = 27.1 bits (57), Expect = 2.1
Identities = 18/49 (36%), Positives = 25/49 (51%), Gaps = 2/49 (4%)
Frame = +3
Query: 114 TTNN-IYGKRNSKT-FAAKRYISSTKMSAKLLNSNLWEADPELFDIIVK 254
T NN +Y R S T F K Y+S K K + + L E++PE + K
Sbjct: 64 TVNNLVYSFRLSPTSFDKKSYMSYIKGYMKAIKARLQESNPERVPVFEK 112
>SPAC30.01c |sec72|sec7b|Sec7 domain|Schizosaccharomyces pombe|chr
1|||Manual
Length = 1822
Score = 26.2 bits (55), Expect = 3.7
Identities = 14/55 (25%), Positives = 32/55 (58%)
Frame = -2
Query: 462 PILICMLPETCSVPVSLFHQYIHSPHSTVDWACLQNICYEDMNSGTEVQELM*NF 298
P++ +L ++P+ LF + IH T+ ++C I E+++ +++EL+ N+
Sbjct: 1758 PVIATILDSILNLPLELFSENIH----TLYFSCCSMIAKENLDD--QLRELLKNY 1806
>SPAC869.08 |pcm2||protein-L-isoaspartate O-methyltransferase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 230
Score = 25.8 bits (54), Expect = 4.9
Identities = 12/49 (24%), Positives = 22/49 (44%)
Frame = +3
Query: 396 EYIDEIEILAQNRSLEAYRLKSEEWGVNVQPYSGSPANFAVYTGIVEPH 542
+++ E + L R+++A S + + PY SP + I PH
Sbjct: 15 QHLVESKFLTNQRAIKAMNATSRSFYCPLSPYMDSPQSIGYGVTISAPH 63
>SPAC20H4.10 |ufd2||ubiquitin-protein ligase E4 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1010
Score = 25.4 bits (53), Expect = 6.5
Identities = 15/58 (25%), Positives = 29/58 (50%)
Frame = +3
Query: 186 MSAKLLNSNLWEADPELFDIIVKEKDRQRAGLEMIASENFTSVPVLQCLSSCLHNKYS 359
++ + LN L E D + + +KD + G ++ +EN +L LS+ +N +S
Sbjct: 84 ITCQALNITLSETDSSKYYLEGFKKDLEEEGSPLLFNENNVDSALLSRLSTTGNNTFS 141
>SPAC3F10.17 |||ribosome biogenesis protein Ltv1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 386
Score = 25.4 bits (53), Expect = 6.5
Identities = 8/20 (40%), Positives = 13/20 (65%)
Frame = +3
Query: 462 EEWGVNVQPYSGSPANFAVY 521
EE+G ++P G AN+ +Y
Sbjct: 57 EEYGSTIRPNEGEAANYGIY 76
>SPBC20F10.08c |||conserved eukaryotic protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 747
Score = 25.0 bits (52), Expect = 8.6
Identities = 12/36 (33%), Positives = 18/36 (50%)
Frame = -2
Query: 129 KYCSLCVLPTITKIFLFLQSRNKNIVLLVCYTSCDI 22
KY S C+LP + +F +++ V T CDI
Sbjct: 246 KYFS-CILPQVWSLFSTQPRLASQLIIAVTNTHCDI 280
>SPAC29E6.03c |uso1|SPAC30.07c|ER to Golgi tethering factor Uso1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1044
Score = 25.0 bits (52), Expect = 8.6
Identities = 12/37 (32%), Positives = 20/37 (54%)
Frame = +3
Query: 135 KRNSKTFAAKRYISSTKMSAKLLNSNLWEADPELFDI 245
K+N+ T + K I ++S K LN NL + + D+
Sbjct: 768 KKNADTESFKNTIREAELSKKALNDNLGNKENIISDL 804
>SPAC2G11.02 |urb2||ribosome biogenesis protein Urb2
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1318
Score = 25.0 bits (52), Expect = 8.6
Identities = 6/17 (35%), Positives = 13/17 (76%)
Frame = -2
Query: 390 PHSTVDWACLQNICYED 340
PHS + W C++++ ++D
Sbjct: 379 PHSKILWECIKSVSHQD 395
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,742,764
Number of Sequences: 5004
Number of extensions: 61240
Number of successful extensions: 177
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 172
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 177
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 266270664
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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