BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmmt22f05
(709 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC1223.07c |||aspartate-tRNA ligase |Schizosaccharomyces pombe... 36 0.004
SPBC1773.10c |||asparagine-tRNA ligase Ded81 |Schizosaccharomyce... 31 0.16
SPAC20G8.02 |||phospholipase|Schizosaccharomyces pombe|chr 1|||M... 29 0.86
SPCC736.06 |||aspartate-tRNA ligase|Schizosaccharomyces pombe|ch... 28 1.1
SPAC27E2.06c |||methionine-tRNA ligase, mitochondrial|Schizosacc... 26 4.6
SPCC1919.12c |||aminopeptidase |Schizosaccharomyces pombe|chr 3|... 25 8.0
>SPCC1223.07c |||aspartate-tRNA ligase |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 580
Score = 36.3 bits (80), Expect = 0.004
Identities = 35/112 (31%), Positives = 52/112 (46%), Gaps = 13/112 (11%)
Frame = +1
Query: 385 RTHT-CGELRPKNVGERVVICGWVQYSRLSK----FLLLRDAYGLAQC--IVGCDNID-- 537
RT+T ++ KN G+ V++ V SRL F LR Y Q +V D I
Sbjct: 90 RTYTQISDISAKNDGQTVLLRARVYTSRLQGNKMCFFSLRQKYDTIQALAVVNKDTISKQ 149
Query: 538 ----LSSLQLESVVQIEGMVSIRPGDTVNPEMATGEIEVTIISLKVLNPQKK 681
S+ LES+V +EG+V P + + E+ I S+ V++P KK
Sbjct: 150 MVKWCGSISLESIVLVEGIVKKSP--EIIKSATVQDAEIHISSIYVISPIKK 199
>SPBC1773.10c |||asparagine-tRNA ligase Ded81 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 568
Score = 31.1 bits (67), Expect = 0.16
Identities = 21/64 (32%), Positives = 31/64 (48%), Gaps = 8/64 (12%)
Frame = +1
Query: 430 RVVICGWVQYSRLSK---FLLLRDAYGLAQCIVGCD-----NIDLSSLQLESVVQIEGMV 585
RV + GWV R K F++LRD G QC++ + D +L ES V + G++
Sbjct: 143 RVRVNGWVHRMRTQKGIIFIILRDGTGFLQCVLSGKVYDRASYDFINLGPESTVCLYGVI 202
Query: 586 SIRP 597
P
Sbjct: 203 KELP 206
>SPAC20G8.02 |||phospholipase|Schizosaccharomyces pombe|chr
1|||Manual
Length = 757
Score = 28.7 bits (61), Expect = 0.86
Identities = 13/28 (46%), Positives = 16/28 (57%)
Frame = +2
Query: 419 MSVKESSYAAGCSIHVCPNFYCFETPTD 502
+S E S+ +G SI C NFY PTD
Sbjct: 585 LSYSEQSFDSGVSILSCQNFYNIFHPTD 612
>SPCC736.06 |||aspartate-tRNA ligase|Schizosaccharomyces pombe|chr
3|||Manual
Length = 611
Score = 28.3 bits (60), Expect = 1.1
Identities = 18/47 (38%), Positives = 25/47 (53%)
Frame = +1
Query: 538 LSSLQLESVVQIEGMVSIRPGDTVNPEMATGEIEVTIISLKVLNPQK 678
LSS+ ESVVQ+ G + RP P E E+ + +K+LN K
Sbjct: 83 LSSIPPESVVQVTGKLQRRPEHDRRP---GNEFELHVEDVKLLNVAK 126
>SPAC27E2.06c |||methionine-tRNA ligase,
mitochondrial|Schizosaccharomyces pombe|chr 1|||Manual
Length = 539
Score = 26.2 bits (55), Expect = 4.6
Identities = 12/36 (33%), Positives = 17/36 (47%)
Frame = +1
Query: 145 TEIVTMLGHKINSNKIKNVCTVFRSTNILKHLCNNN 252
TE++ LG N ++N VF T H NN+
Sbjct: 496 TELLDQLGIPKNQRSLQNATNVFEPTEFTFHSGNNS 531
>SPCC1919.12c |||aminopeptidase |Schizosaccharomyces pombe|chr
3|||Manual
Length = 843
Score = 25.4 bits (53), Expect = 8.0
Identities = 11/33 (33%), Positives = 18/33 (54%)
Frame = -3
Query: 704 IVF*VECYFFCGFKTFKLIMVTSISPVAISGFT 606
+ F V C F F T ++++T I+P I+ T
Sbjct: 387 VCFVVSCIFVSIFDTLTVLLLTWINPYVINSHT 419
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,842,166
Number of Sequences: 5004
Number of extensions: 58205
Number of successful extensions: 139
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 135
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 139
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 329179816
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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