Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= bmmt22e10
         (654 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC16E8.17c |||succinate-CoA ligase alpha subunit|Schizosacchar...   256   2e-69
SPBP4H10.19c |||calreticulin/calnexin homolog|Schizosaccharomyce...    28   1.4  
SPBC28F2.12 |rpb1||DNA-directed RNA polymerase II large subunit|...    27   1.8  
SPAC3G6.09c |tps2||trehalose-phosphate synthase Tps2 |Schizosacc...    27   3.1  
SPCC1235.05c |fft2||fun thirty related protein Fft2|Schizosaccha...    26   4.1  
SPBC16C6.02c |vps1302|vps13b|chorein homolog|Schizosaccharomyces...    25   7.2  
SPAC31A2.05c |mis4||cohesin loading factor Mis4|Schizosaccharomy...    25   7.2  
SPAC26H5.03 |||WD repeat protein Cac2|Schizosaccharomyces pombe|...    25   9.5  

>SPAC16E8.17c |||succinate-CoA ligase alpha
           subunit|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 331

 Score =  256 bits (628), Expect = 2e-69
 Identities = 124/202 (61%), Positives = 149/202 (73%)
 Frame = +1

Query: 46  LSKFKDGLKLGNVRFASGNPYAETRKNLILTSETKVIVQGFTGKQGTFHSQQALDYGTKV 225
           L +F    +L N    S + Y +T  NL++ S+TKVI QGFTGKQGTFH+Q A+DYGTKV
Sbjct: 12  LRRFSSSSQLKN----SKSLYEQTIPNLMINSDTKVIFQGFTGKQGTFHAQHAMDYGTKV 67

Query: 226 VGGVSPKKAGTEHLGKPVFGTVKEAKAGTGATASVIYVPPPGXXXXXXXXXXXXMPLIVC 405
           VGG +PKKAGT HLGKPVFGT++EA   T A AS ++VPPP             +PLIV 
Sbjct: 68  VGGTNPKKAGTTHLGKPVFGTIEEAMKETKADASAVFVPPPLAAGAIEEAIAAEVPLIVA 127

Query: 406 ITEGVPQHDMVRVKHALLRQNKSRLVGPNCPGIIAPEKCKIGIMPAAVHKRGCIGVVSRS 585
           ITEG+PQHDM+RV   L  Q+KSRLVGPNCPGII P +CKIGIMP+ +HK GCIG+VSRS
Sbjct: 128 ITEGIPQHDMLRVSDILKTQSKSRLVGPNCPGIIRPGQCKIGIMPSHIHKPGCIGIVSRS 187

Query: 586 GTLTYEACHQTTITGLGQTLCV 651
           GTLTYEA +QTT T LGQ+L +
Sbjct: 188 GTLTYEAVNQTTQTDLGQSLVI 209


>SPBP4H10.19c |||calreticulin/calnexin homolog|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 381

 Score = 27.9 bits (59), Expect = 1.4
 Identities = 16/50 (32%), Positives = 22/50 (44%)
 Frame = -1

Query: 525 FTLLGSNDTRTVRTNQSRLVLSQEGMFYSHHIMLWHTFCNAHNEGHFSLN 376
           +TLL  N T  +R +QS +     G  + H    W T  N  N  + S N
Sbjct: 186 YTLLLQNTTVVIRRDQSVVYTGDVGTNFFHSPTKWITHSNVSNGYYTSRN 235


>SPBC28F2.12 |rpb1||DNA-directed RNA polymerase II large
           subunit|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1752

 Score = 27.5 bits (58), Expect = 1.8
 Identities = 20/70 (28%), Positives = 32/70 (45%), Gaps = 1/70 (1%)
 Frame = +1

Query: 103 PYAETRKNLILTSETKVIVQGFTGKQGTFHSQQALDYGTKVVG-GVSPKKAGTEHLGKPV 279
           PY+  R NL +TS        F G +   H  Q+ +   ++    + PK+  +    KPV
Sbjct: 470 PYSTFRLNLSVTSPYNA---DFDGDEMNMHVPQSEETRAEIQEITMVPKQIVSPQSNKPV 526

Query: 280 FGTVKEAKAG 309
            G V++  AG
Sbjct: 527 MGIVQDTLAG 536


>SPAC3G6.09c |tps2||trehalose-phosphate synthase Tps2
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 849

 Score = 26.6 bits (56), Expect = 3.1
 Identities = 14/44 (31%), Positives = 24/44 (54%)
 Frame = -1

Query: 261 FCTSLLW*HSSNNFSTIVKGLLAVEGTLLSSETLNNHFCFTCKD 130
           FCTS ++   S   + I+KG+LA     + +++   HF  TC +
Sbjct: 252 FCTSEVFRCLSKR-AEILKGVLASNVIAMQTDSYTRHFLSTCSN 294


>SPCC1235.05c |fft2||fun thirty related protein
           Fft2|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 1284

 Score = 26.2 bits (55), Expect = 4.1
 Identities = 10/34 (29%), Positives = 20/34 (58%)
 Frame = -2

Query: 314 PVPAFASLTVPNTGLPRCSVPAFFGDTPPTTLVP 213
           P+P++ + ++PN G+P  ++P      P T  +P
Sbjct: 124 PLPSYNTASLPNAGIP-AAMPGMPSGYPGTVPIP 156


>SPBC16C6.02c |vps1302|vps13b|chorein homolog|Schizosaccharomyces
            pombe|chr 2|||Manual
          Length = 3131

 Score = 25.4 bits (53), Expect = 7.2
 Identities = 13/69 (18%), Positives = 32/69 (46%)
 Frame = +1

Query: 4    FTFNNSTMAMPVRVLSKFKDGLKLGNVRFASGNPYAETRKNLILTSETKVIVQGFTGKQG 183
            +  NN+     V+V   F+  +KLG V        ++T++N +++     ++  F   + 
Sbjct: 801  YKLNNTPSEQSVKVSHFFEFNMKLGEVTLILCREDSQTKRNSMVSVNFAKLLLSFNQFED 860

Query: 184  TFHSQQALD 210
              H + +++
Sbjct: 861  NSHLRMSIN 869


>SPAC31A2.05c |mis4||cohesin loading factor Mis4|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 1583

 Score = 25.4 bits (53), Expect = 7.2
 Identities = 18/65 (27%), Positives = 29/65 (44%), Gaps = 3/65 (4%)
 Frame = -1

Query: 309 ACLCLFDCTKHRLTKMFCTSLLW*HS---SNNFSTIVKGLLAVEGTLLSSETLNNHFCFT 139
           A L L     +++  +F  SL   H+     NF+ I+  + ++      S+   NHF F 
Sbjct: 632 AALDLISLIVNKVLALFDLSLFEKHNIPAPTNFNDIISFIPSITRLNELSQVSFNHFYFL 691

Query: 138 CKDQI 124
           CK  I
Sbjct: 692 CKGDI 696


>SPAC26H5.03 |||WD repeat protein Cac2|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 512

 Score = 25.0 bits (52), Expect = 9.5
 Identities = 10/30 (33%), Positives = 15/30 (50%)
 Frame = +2

Query: 20  LQWLCQSGFFLSSKMD*NSAMYDLHPATLM 109
           L W   S F ++  MD +  +YD H   L+
Sbjct: 134 LCWSVDSNFLIAGAMDNSLRLYDAHTGQLL 163


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,742,376
Number of Sequences: 5004
Number of extensions: 57106
Number of successful extensions: 158
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 148
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 157
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 295793106
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)