Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= bmmt22d05
         (728 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPCC306.08c |||malate dehydrogenase|Schizosaccharomyces pombe|ch...   185   5e-48
SPAC186.08c |||L-lactate dehydrogenase |Schizosaccharomyces pomb...    62   6e-11
SPAC23H3.05c |swd1||COMPASS complex subunit Swd1|Schizosaccharom...    28   1.2  
SPBC3B8.07c |dsd1|SDCB3B8.07c|dihydroceramide delta-4 desaturase...    27   2.7  
SPAC11D3.06 |||MatE family transporter|Schizosaccharomyces pombe...    26   4.8  
SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family ...    26   6.3  
SPAC16C9.04c |||CCR4-Not complex subunit Mot2 |Schizosaccharomyc...    26   6.3  
SPBC1683.05 |||thiamine transporter |Schizosaccharomyces pombe|c...    25   8.4  
SPAC19A8.07c |||U3 snoRNP-associated protein Imp4 |Schizosacchar...    25   8.4  
SPBC30D10.10c |tor1||phosphatidylinositol kinase Tor1|Schizosacc...    25   8.4  

>SPCC306.08c |||malate dehydrogenase|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 341

 Score =  185 bits (451), Expect = 5e-48
 Identities = 94/175 (53%), Positives = 122/175 (69%), Gaps = 2/175 (1%)
 Frame = +1

Query: 136 KNFSTTSQRNFKXXXXXXXXXIGQPLALLLKQNPLVTRLALYDIAPVTPGVAADLSHMNT 315
           ++FSTTS R FK         IGQPL++LLK N  V+ LAL+DI    PGVAAD+ H+NT
Sbjct: 19  RSFSTTSSRAFKVAVLGAGGGIGQPLSMLLKLNDKVSELALFDIRGA-PGVAADIGHINT 77

Query: 316 PAKVSGHKGPEE-LSAAIKDADVVVIPAGVPRKPGMTRDDLFNTNASIVRDIALSIAQNA 492
            + V G+   ++ L  A+  ADVV+IPAGVPRKPGMTRDDLF TNASIVRD+A +  +  
Sbjct: 78  TSNVVGYAPDDKGLEKALNGADVVIIPAGVPRKPGMTRDDLFATNASIVRDLAFAAGETC 137

Query: 493 PKAIVAIITNPVNSTVPIASEVLKKAGVYDPNRVLGVTTLDVVRAATFVGEI-NG 654
           P+A   ++TNPVNSTVPI  + L++ GV+ P  + GVTTLD VRA+ F  ++ NG
Sbjct: 138 PEAKYLVVTNPVNSTVPIFKKALERVGVHQPKHLFGVTTLDSVRASRFTSQVTNG 192



 Score = 35.5 bits (78), Expect = 0.008
 Identities = 14/22 (63%), Positives = 18/22 (81%)
 Frame = +3

Query: 663 DLRRRPVIGGHSGITIIPILSQ 728
           +L   PV+GGHSG TI+P+LSQ
Sbjct: 195 ELLHIPVVGGHSGATIVPLLSQ 216


>SPAC186.08c |||L-lactate dehydrogenase |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 330

 Score = 62.5 bits (145), Expect = 6e-11
 Identities = 58/185 (31%), Positives = 83/185 (44%), Gaps = 8/185 (4%)
 Frame = +1

Query: 130 GAKNFSTTSQRN--FKXXXXXXXXX--IGQPLALLLKQNPLVTRLALYDI-APVTPGVAA 294
           G+K FS  S R+  FK           +G   A  L  + L   + + D+      G A 
Sbjct: 4   GSKVFSNDSVRSSSFKSIKIVIVGAGNVGSTTAFTLLLSGLAAEIVIIDLNKKKAEGEAM 63

Query: 295 DLSHMNTPAKVSGHKGPEELS--AAIKDADVVVIPAGVPRKPGMTRDDLFNTNASIVRDI 468
           DL+H    A    H+    L      KDA  VVI AG  +KPG TR DL   N SI ++I
Sbjct: 64  DLNH----AAPLSHETRVYLGDYKDCKDATAVVITAGKNQKPGETRMDLLKANISIFKEI 119

Query: 469 ALSIAQNAPKAIVAIITNPVNSTVPIASEVLKKAGVYDPNRVLGV-TTLDVVRAATFVGE 645
              + +    AI+ + TNPV+    +    LK  G +   RV+G  T +D  R    +G+
Sbjct: 120 LREVTKYTKDAILLVATNPVD---VLTYATLKLTG-FPAERVIGSGTIIDTARFQYLIGK 175

Query: 646 INGVD 660
           + G+D
Sbjct: 176 LYGLD 180


>SPAC23H3.05c |swd1||COMPASS complex subunit
           Swd1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 398

 Score = 28.3 bits (60), Expect = 1.2
 Identities = 13/32 (40%), Positives = 17/32 (53%)
 Frame = -3

Query: 486 LSNGQSNVTDNGSISVE*VITGHSRFTGYSCW 391
           L NG   + D  + SV  V+TGH+R     CW
Sbjct: 43  LVNGSVVIWDLSTFSVSRVLTGHTRAIQSVCW 74


>SPBC3B8.07c |dsd1|SDCB3B8.07c|dihydroceramide delta-4
           desaturase|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 362

 Score = 27.1 bits (57), Expect = 2.7
 Identities = 10/26 (38%), Positives = 14/26 (53%)
 Frame = +3

Query: 114 PCCTKWCKELFHHITEEL*SGGRWCR 191
           P C  WC  ++  IT+   + G WCR
Sbjct: 319 PDCKSWCGIIYQFITDS--NVGMWCR 342


>SPAC11D3.06 |||MatE family transporter|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 455

 Score = 26.2 bits (55), Expect = 4.8
 Identities = 15/36 (41%), Positives = 18/36 (50%)
 Frame = +1

Query: 256 LYDIAPVTPGVAADLSHMNTPAKVSGHKGPEELSAA 363
           L + APV  G A   S   +   V+G  GP ELS A
Sbjct: 12  LINSAPVILGYALQNSLQTSSVIVTGRLGPSELSVA 47


>SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1420

 Score = 25.8 bits (54), Expect = 6.3
 Identities = 11/26 (42%), Positives = 15/26 (57%)
 Frame = +1

Query: 580 DPNRVLGVTTLDVVRAATFVGEINGV 657
           D  R LG+   D++R    V E+NGV
Sbjct: 835 DTLRTLGLKEGDIIRVMNHVNELNGV 860


>SPAC16C9.04c |||CCR4-Not complex subunit Mot2 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 489

 Score = 25.8 bits (54), Expect = 6.3
 Identities = 13/33 (39%), Positives = 18/33 (54%)
 Frame = +3

Query: 102 KTCRPCCTKWCKELFHHITEEL*SGGRWCRRWY 200
           K C+ C  + C+  +HHI E+L      CRR Y
Sbjct: 33  KPCQ-CGYRVCRFCWHHIKEDLNGRCPACRRLY 64


>SPBC1683.05 |||thiamine transporter |Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 559

 Score = 25.4 bits (53), Expect = 8.4
 Identities = 12/45 (26%), Positives = 24/45 (53%)
 Frame = +1

Query: 502 IVAIITNPVNSTVPIASEVLKKAGVYDPNRVLGVTTLDVVRAATF 636
           +VA ++ P+     +AS+++    ++DP ++    T    RAA F
Sbjct: 307 LVAAVSAPIGIVSGVASKIMYGTAMWDPLQIANNWTSRGGRAAAF 351


>SPAC19A8.07c |||U3 snoRNP-associated protein Imp4
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 289

 Score = 25.4 bits (53), Expect = 8.4
 Identities = 10/16 (62%), Positives = 11/16 (68%)
 Frame = -2

Query: 67  RVYTCIFELFPPDPPD 20
           RV T +  LFPPDP D
Sbjct: 199 RVKTALSALFPPDPKD 214


>SPBC30D10.10c |tor1||phosphatidylinositol kinase
           Tor1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 2335

 Score = 25.4 bits (53), Expect = 8.4
 Identities = 13/43 (30%), Positives = 24/43 (55%)
 Frame = +1

Query: 238 LVTRLALYDIAPVTPGVAADLSHMNTPAKVSGHKGPEELSAAI 366
           ++ RLALY+ A V P +   +  + +  + SG+   +E SA +
Sbjct: 586 IIGRLALYNPAHVMPSLRKTIIQLLSDMEYSGNSRQKEESAQL 628


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.316    0.132    0.366 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,893,401
Number of Sequences: 5004
Number of extensions: 61981
Number of successful extensions: 158
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 149
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 156
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 343230174
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.6 bits)