Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmmt22b14
(714 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC2F3.09 |hem1||5-aminolevulinate synthase|Schizosaccharomyces... 110 2e-25
SPAC21E11.08 |lcb2|SPAC2C4.02|serine palmitoyltransferase |Schiz... 99 3e-22
SPBC18E5.02c ||SPBC29A3.20c|serine palmitoyltransferase complex ... 57 2e-09
SPAC2F7.16c |||phospholipase D |Schizosaccharomyces pombe|chr 1|... 29 0.50
SPBP23A10.04 |apc2||anaphase-promoting complex subunit Apc2 |Sch... 28 1.2
SPBC1289.16c ||SPBC8E4.06|copper amine oxidase |Schizosaccharomy... 26 4.7
SPAC22F3.12c |rgs1||regulator of G-protein signaling Rgs1|Schizo... 26 6.1
SPCC736.13 |||short chain dehydrogenase|Schizosaccharomyces pomb... 26 6.1
SPAC3H5.09c |||conserved fungal protein|Schizosaccharomyces pomb... 25 8.1
SPCC285.10c |||SPRY domain protein|Schizosaccharomyces pombe|chr... 25 8.1
SPCC550.14 |||vigilin |Schizosaccharomyces pombe|chr 3|||Manual 25 8.1
>SPAC2F3.09 |hem1||5-aminolevulinate synthase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 558
Score = 110 bits (264), Expect = 2e-25
Identities = 58/160 (36%), Positives = 87/160 (54%), Gaps = 3/160 (1%)
Frame = +3
Query: 240 FCANNYLGLSNHPEVVEAAREGLKKYGAGLSSVRFICGTQSIHKELENRLSQFHGREDTI 419
+C+N+YL + H ++ EA + ++ YG G R I G LE L+ H + +
Sbjct: 179 WCSNDYLNMGGHKKIREAMHQCIETYGGGAGGTRNIAGHNQHAVRLEKSLADLHQKPAAL 238
Query: 420 LYGSCFDANAGLFESM--LTPEDAVFSDALNHASIIDGIRLCKAQKYRYPHRDLTELEHL 593
++GSC+ AN ++ P SD +NHAS+I+GIR + +K + H DL +LE
Sbjct: 239 VFGSCYVANDATLSTLGRKLPNCIFLSDEMNHASMINGIRNSRCEKIIFKHNDLVDLEAK 298
Query: 594 LAHSEA-RIKLIVTDGVFSMDGTVAPIKGLXDLADKYRAL 710
LA R K+I + V+SM G VAPI + DLA KY A+
Sbjct: 299 LASLPLNRPKIIAFESVYSMSGNVAPISEICDLAKKYGAI 338
>SPAC21E11.08 |lcb2|SPAC2C4.02|serine palmitoyltransferase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 603
Score = 99 bits (238), Expect = 3e-22
Identities = 56/188 (29%), Positives = 93/188 (49%), Gaps = 11/188 (5%)
Frame = +3
Query: 183 TSPQDTKVRVQGAQGEFLNFCANNYLGLS-NHPEVVEAAREGLKKYGAGLSSVRFICGTQ 359
++ ++ ++ G LN + NYLG + +H E ++KYG S ICGT
Sbjct: 173 STDSNSTFKLTGDTSLALNVSSYNYLGFAQSHGPCATKVEEAMQKYGLSTCSSNAICGTY 232
Query: 360 SIHKELENRLSQFHGREDTILYGSCFDANAGLFESMLTPEDAVFSDALNHASIIDGIRLC 539
+HKE+E + F G+ +++ F NA +F +++ P + SD LNH SI G RL
Sbjct: 233 GLHKEVEELTANFVGKPAALVFSQGFSTNATVFSTLMCPGSLIISDELNHTSIRFGARLS 292
Query: 540 KAQKYRYPHRDLTELEHLL----------AHSEARIKLIVTDGVFSMDGTVAPIKGLXDL 689
A Y H D+T+LE +L H L+V +G++SM+G + + +L
Sbjct: 293 GANIRVYKHNDMTDLERVLREVISQGQPRTHRPYSKILVVIEGLYSMEGNFCDLPKVVEL 352
Query: 690 ADKYRALL 713
++Y+ L
Sbjct: 353 KNRYKFYL 360
>SPBC18E5.02c ||SPBC29A3.20c|serine palmitoyltransferase complex
subunit |Schizosaccharomyces pombe|chr 2|||Manual
Length = 509
Score = 57.2 bits (132), Expect = 2e-09
Identities = 39/165 (23%), Positives = 71/165 (43%), Gaps = 9/165 (5%)
Frame = +3
Query: 237 NFCANNYLGLSNHPEVVEAAREGLKKYGAGLSSVRFICGTQSIHKELENRLSQFHGREDT 416
NF + N+L L+ + + E A L++ G G GTQ H LE ++ F G E
Sbjct: 131 NFASFNFLDLAENKHITECAVATLRECGLGACGPPGFYGTQDKHLRLEKDIASFIGVERA 190
Query: 417 ILYGSCFDANAGLFESMLTPEDAVFSDALNHASIIDGIRLCKAQKYRYPHRDLTELEHLL 596
I+Y F + + + D + D + +I GI++ + + H ++ +LE +L
Sbjct: 191 IVYAQSFQTISSVIPAFSKRGDILVVDEACNFAIQKGIQISRTTIRYFKHNNMKDLERIL 250
Query: 597 AHSEARI---------KLIVTDGVFSMDGTVAPIKGLXDLADKYR 704
E + I+T+G+ G + + + L KY+
Sbjct: 251 QELEDDFVKHNRPLTRRFIITEGISENYGDMVDLTKIVALKKKYK 295
>SPAC2F7.16c |||phospholipase D |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1369
Score = 29.5 bits (63), Expect = 0.50
Identities = 33/144 (22%), Positives = 53/144 (36%), Gaps = 7/144 (4%)
Frame = +3
Query: 81 KRQERAGVAKLRDVLEDR---LQEIKRAKTWKHERVLTSPQDTKVRVQGAQGEFLNFCAN 251
KR R + DVLE R + + R WK V T + + E L
Sbjct: 1007 KRLMREHLGLETDVLEQREYNMDGLDRDTEWKRVEVWTPDEGNAINGSAYTAEELKMKYR 1066
Query: 252 NYLGLSNHPEVVEAAREGLKKYGAGL----SSVRFICGTQSIHKELENRLSQFHGREDTI 419
+ + P+++ A + +KK + SS+ F TQ E E + D I
Sbjct: 1067 SQSQFTTTPDILRKAEKSMKKLDQRVSLIPSSIEFNIKTQKDKVEFEKNYEKSKKGPDVI 1126
Query: 420 LYGSCFDANAGLFESMLTPEDAVF 491
G+ S+ T ED+++
Sbjct: 1127 ANALV----GGIPLSLKTKEDSLY 1146
>SPBP23A10.04 |apc2||anaphase-promoting complex subunit Apc2
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 681
Score = 28.3 bits (60), Expect = 1.2
Identities = 14/44 (31%), Positives = 24/44 (54%), Gaps = 1/44 (2%)
Frame = +3
Query: 102 VAKLRDVLEDRLQEIKR-AKTWKHERVLTSPQDTKVRVQGAQGE 230
+ K ++L+ + ++R K W H RVLT D + RV+ + E
Sbjct: 545 IEKAAELLDQPKEIVERHLKFWLHHRVLTDIGDDRYRVRETEAE 588
>SPBC1289.16c ||SPBC8E4.06|copper amine oxidase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 794
Score = 26.2 bits (55), Expect = 4.7
Identities = 13/36 (36%), Positives = 20/36 (55%)
Frame = +3
Query: 138 QEIKRAKTWKHERVLTSPQDTKVRVQGAQGEFLNFC 245
QE++ K K R+ T P+ R++G E+ NFC
Sbjct: 221 QEMEAMKMLKPLRI-TQPEGVNFRIKGRYIEWQNFC 255
>SPAC22F3.12c |rgs1||regulator of G-protein signaling
Rgs1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 481
Score = 25.8 bits (54), Expect = 6.1
Identities = 13/32 (40%), Positives = 18/32 (56%)
Frame = +1
Query: 160 LGNMRGCSHRRKTLRCGSKVLKENS*TSVPTT 255
L N++ R RCGSKVLK + ++P T
Sbjct: 88 LENLQLSQVNRIKSRCGSKVLKSTTKFTIPKT 119
>SPCC736.13 |||short chain dehydrogenase|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 339
Score = 25.8 bits (54), Expect = 6.1
Identities = 32/134 (23%), Positives = 60/134 (44%)
Frame = +3
Query: 123 LEDRLQEIKRAKTWKHERVLTSPQDTKVRVQGAQGEFLNFCANNYLGLSNHPEVVEAARE 302
L +R+ E T K L +P+ T + G+ ++ G + +E AR+
Sbjct: 8 LLNRVNESAIVNTLKEYTGLNTPKWTFNDIPDLTGKVALVTGSS--GGIGYVTALELARK 65
Query: 303 GLKKYGAGLSSVRFICGTQSIHKELENRLSQFHGREDTILYGSCFDANAGLFESMLTPED 482
G K Y AG + ++ + IH E+ + +F R D + + S + A ES + E+
Sbjct: 66 GAKVYLAGRNEEKYQKVMKQIHDEVRHSKIRFL-RLDLLDFESVYQA----AESFIAKEE 120
Query: 483 AVFSDALNHASIID 524
+ +N+A I++
Sbjct: 121 KLHI-LVNNAGIMN 133
>SPAC3H5.09c |||conserved fungal protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 2685
Score = 25.4 bits (53), Expect = 8.1
Identities = 13/53 (24%), Positives = 22/53 (41%)
Frame = +3
Query: 459 ESMLTPEDAVFSDALNHASIIDGIRLCKAQKYRYPHRDLTELEHLLAHSEARI 617
E + P+ F D H ++ KA +Y + ELE ++ + RI
Sbjct: 1714 EKEIIPDTCRFGDEFTHRCLMPSRENPKAVQYELLQKRRKELEEFMSSEQERI 1766
>SPCC285.10c |||SPRY domain protein|Schizosaccharomyces pombe|chr
3|||Manual
Length = 382
Score = 25.4 bits (53), Expect = 8.1
Identities = 10/29 (34%), Positives = 17/29 (58%)
Frame = -3
Query: 511 AWFKASENTASSGVSMDSNRPALASKQDP 425
+W ASE++A + D+NRP + + P
Sbjct: 317 SWDAASESSAGTTTQGDTNRPDKSKNRSP 345
>SPCC550.14 |||vigilin |Schizosaccharomyces pombe|chr 3|||Manual
Length = 1279
Score = 25.4 bits (53), Expect = 8.1
Identities = 11/22 (50%), Positives = 16/22 (72%)
Frame = +3
Query: 78 VKRQERAGVAKLRDVLEDRLQE 143
V + RAGV K +D++ +RLQE
Sbjct: 1253 VVQGSRAGVVKAKDLIFERLQE 1274
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,764,231
Number of Sequences: 5004
Number of extensions: 52401
Number of successful extensions: 182
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 173
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 180
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 333194204
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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