Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmmt20o06
(696 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC2F3.09 |hem1||5-aminolevulinate synthase|Schizosaccharomyces... 103 2e-23
SPAC21E11.08 |lcb2|SPAC2C4.02|serine palmitoyltransferase |Schiz... 94 1e-20
SPBC18E5.02c ||SPBC29A3.20c|serine palmitoyltransferase complex ... 55 1e-08
SPAC2F7.16c |||phospholipase D |Schizosaccharomyces pombe|chr 1|... 29 0.48
SPBP23A10.04 |apc2||anaphase-promoting complex subunit Apc2 |Sch... 28 1.1
SPBC1289.16c ||SPBC8E4.06|copper amine oxidase |Schizosaccharomy... 26 4.5
SPAC22F3.12c |rgs1||regulator of G-protein signaling Rgs1|Schizo... 26 5.9
SPCC736.13 |||short chain dehydrogenase|Schizosaccharomyces pomb... 26 5.9
SPAC3H5.09c |||conserved fungal protein|Schizosaccharomyces pomb... 25 7.8
SPCC285.10c |||SPRY domain protein|Schizosaccharomyces pombe|chr... 25 7.8
SPCC550.14 |||vigilin |Schizosaccharomyces pombe|chr 3|||Manual 25 7.8
>SPAC2F3.09 |hem1||5-aminolevulinate synthase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 558
Score = 103 bits (247), Expect = 2e-23
Identities = 55/154 (35%), Positives = 83/154 (53%), Gaps = 3/154 (1%)
Frame = +3
Query: 243 FCANNYLGLSNHPEVVEAAREGLKKYGAGLSSVRFICGTQSIHKELENRLSQFHGREDTI 422
+C+N+YL + H ++ EA + ++ YG G R I G LE L+ H + +
Sbjct: 179 WCSNDYLNMGGHKKIREAMHQCIETYGGGAGGTRNIAGHNQHAVRLEKSLADLHQKPAAL 238
Query: 423 LYGSCFDANAGLFESM--LTPEDAVFSDALNHASIIDGIRLCKAQKYRYPHRDLTELEHL 596
++GSC+ AN ++ P SD +NHAS+I+GIR + +K + H DL +LE
Sbjct: 239 VFGSCYVANDATLSTLGRKLPNCIFLSDEMNHASMINGIRNSRCEKIIFKHNDLVDLEAK 298
Query: 597 LAHSEA-RIKLIVTDGVFSMDGTVAPIKGLRDLA 695
LA R K+I + V+SM G VAPI + DLA
Sbjct: 299 LASLPLNRPKIIAFESVYSMSGNVAPISEICDLA 332
>SPAC21E11.08 |lcb2|SPAC2C4.02|serine palmitoyltransferase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 603
Score = 94.3 bits (224), Expect = 1e-20
Identities = 54/180 (30%), Positives = 88/180 (48%), Gaps = 11/180 (6%)
Frame = +3
Query: 186 TSPQDTKVRVQGAQGEFLNFCANNYLGLS-NHPEVVEAAREGLKKYGAGLSSVRFICGTQ 362
++ ++ ++ G LN + NYLG + +H E ++KYG S ICGT
Sbjct: 173 STDSNSTFKLTGDTSLALNVSSYNYLGFAQSHGPCATKVEEAMQKYGLSTCSSNAICGTY 232
Query: 363 SIHKELENRLSQFHGREDTILYGSCFDANAGLFESMLTPEDAVFSDALNHASIIDGIRLC 542
+HKE+E + F G+ +++ F NA +F +++ P + SD LNH SI G RL
Sbjct: 233 GLHKEVEELTANFVGKPAALVFSQGFSTNATVFSTLMCPGSLIISDELNHTSIRFGARLS 292
Query: 543 KAQKYRYPHRDLTELEHLL----------AHSEARIKLIVTDGVFSMDGTVAPIKGLRDL 692
A Y H D+T+LE +L H L+V +G++SM+G + + +L
Sbjct: 293 GANIRVYKHNDMTDLERVLREVISQGQPRTHRPYSKILVVIEGLYSMEGNFCDLPKVVEL 352
>SPBC18E5.02c ||SPBC29A3.20c|serine palmitoyltransferase complex
subunit |Schizosaccharomyces pombe|chr 2|||Manual
Length = 509
Score = 54.8 bits (126), Expect = 1e-08
Identities = 32/124 (25%), Positives = 56/124 (45%)
Frame = +3
Query: 240 NFCANNYLGLSNHPEVVEAAREGLKKYGAGLSSVRFICGTQSIHKELENRLSQFHGREDT 419
NF + N+L L+ + + E A L++ G G GTQ H LE ++ F G E
Sbjct: 131 NFASFNFLDLAENKHITECAVATLRECGLGACGPPGFYGTQDKHLRLEKDIASFIGVERA 190
Query: 420 ILYGSCFDANAGLFESMLTPEDAVFSDALNHASIIDGIRLCKAQKYRYPHRDLTELEHLL 599
I+Y F + + + D + D + +I GI++ + + H ++ +LE +L
Sbjct: 191 IVYAQSFQTISSVIPAFSKRGDILVVDEACNFAIQKGIQISRTTIRYFKHNNMKDLERIL 250
Query: 600 AHSE 611
E
Sbjct: 251 QELE 254
>SPAC2F7.16c |||phospholipase D |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1369
Score = 29.5 bits (63), Expect = 0.48
Identities = 33/144 (22%), Positives = 53/144 (36%), Gaps = 7/144 (4%)
Frame = +3
Query: 84 KRQERAGVAKLRDVLEDR---LQEIKRAKTWKHERVLTSPQDTKVRVQGAQGEFLNFCAN 254
KR R + DVLE R + + R WK V T + + E L
Sbjct: 1007 KRLMREHLGLETDVLEQREYNMDGLDRDTEWKRVEVWTPDEGNAINGSAYTAEELKMKYR 1066
Query: 255 NYLGLSNHPEVVEAAREGLKKYGAGL----SSVRFICGTQSIHKELENRLSQFHGREDTI 422
+ + P+++ A + +KK + SS+ F TQ E E + D I
Sbjct: 1067 SQSQFTTTPDILRKAEKSMKKLDQRVSLIPSSIEFNIKTQKDKVEFEKNYEKSKKGPDVI 1126
Query: 423 LYGSCFDANAGLFESMLTPEDAVF 494
G+ S+ T ED+++
Sbjct: 1127 ANALV----GGIPLSLKTKEDSLY 1146
>SPBP23A10.04 |apc2||anaphase-promoting complex subunit Apc2
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 681
Score = 28.3 bits (60), Expect = 1.1
Identities = 14/44 (31%), Positives = 24/44 (54%), Gaps = 1/44 (2%)
Frame = +3
Query: 105 VAKLRDVLEDRLQEIKR-AKTWKHERVLTSPQDTKVRVQGAQGE 233
+ K ++L+ + ++R K W H RVLT D + RV+ + E
Sbjct: 545 IEKAAELLDQPKEIVERHLKFWLHHRVLTDIGDDRYRVRETEAE 588
>SPBC1289.16c ||SPBC8E4.06|copper amine oxidase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 794
Score = 26.2 bits (55), Expect = 4.5
Identities = 13/36 (36%), Positives = 20/36 (55%)
Frame = +3
Query: 141 QEIKRAKTWKHERVLTSPQDTKVRVQGAQGEFLNFC 248
QE++ K K R+ T P+ R++G E+ NFC
Sbjct: 221 QEMEAMKMLKPLRI-TQPEGVNFRIKGRYIEWQNFC 255
>SPAC22F3.12c |rgs1||regulator of G-protein signaling
Rgs1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 481
Score = 25.8 bits (54), Expect = 5.9
Identities = 13/32 (40%), Positives = 18/32 (56%)
Frame = +1
Query: 163 LGNMRGCSHRRKTLRCGSKVLKENS*TSVPTT 258
L N++ R RCGSKVLK + ++P T
Sbjct: 88 LENLQLSQVNRIKSRCGSKVLKSTTKFTIPKT 119
>SPCC736.13 |||short chain dehydrogenase|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 339
Score = 25.8 bits (54), Expect = 5.9
Identities = 32/134 (23%), Positives = 60/134 (44%)
Frame = +3
Query: 126 LEDRLQEIKRAKTWKHERVLTSPQDTKVRVQGAQGEFLNFCANNYLGLSNHPEVVEAARE 305
L +R+ E T K L +P+ T + G+ ++ G + +E AR+
Sbjct: 8 LLNRVNESAIVNTLKEYTGLNTPKWTFNDIPDLTGKVALVTGSS--GGIGYVTALELARK 65
Query: 306 GLKKYGAGLSSVRFICGTQSIHKELENRLSQFHGREDTILYGSCFDANAGLFESMLTPED 485
G K Y AG + ++ + IH E+ + +F R D + + S + A ES + E+
Sbjct: 66 GAKVYLAGRNEEKYQKVMKQIHDEVRHSKIRFL-RLDLLDFESVYQA----AESFIAKEE 120
Query: 486 AVFSDALNHASIID 527
+ +N+A I++
Sbjct: 121 KLHI-LVNNAGIMN 133
>SPAC3H5.09c |||conserved fungal protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 2685
Score = 25.4 bits (53), Expect = 7.8
Identities = 13/53 (24%), Positives = 22/53 (41%)
Frame = +3
Query: 462 ESMLTPEDAVFSDALNHASIIDGIRLCKAQKYRYPHRDLTELEHLLAHSEARI 620
E + P+ F D H ++ KA +Y + ELE ++ + RI
Sbjct: 1714 EKEIIPDTCRFGDEFTHRCLMPSRENPKAVQYELLQKRRKELEEFMSSEQERI 1766
>SPCC285.10c |||SPRY domain protein|Schizosaccharomyces pombe|chr
3|||Manual
Length = 382
Score = 25.4 bits (53), Expect = 7.8
Identities = 10/29 (34%), Positives = 17/29 (58%)
Frame = -3
Query: 514 AWFKASENTASSGVSMDSNRPALASKQDP 428
+W ASE++A + D+NRP + + P
Sbjct: 317 SWDAASESSAGTTTQGDTNRPDKSKNRSP 345
>SPCC550.14 |||vigilin |Schizosaccharomyces pombe|chr 3|||Manual
Length = 1279
Score = 25.4 bits (53), Expect = 7.8
Identities = 11/22 (50%), Positives = 16/22 (72%)
Frame = +3
Query: 81 VKRQERAGVAKLRDVLEDRLQE 146
V + RAGV K +D++ +RLQE
Sbjct: 1253 VVQGSRAGVVKAKDLIFERLQE 1274
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,733,285
Number of Sequences: 5004
Number of extensions: 52089
Number of successful extensions: 184
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 175
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 182
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 321151040
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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