Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= bmmt20h02
         (698 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC19A8.04 |erg5||C-22 sterol desaturase Erg5 |Schizosaccharomy...    55   9e-09
SPAC13A11.02c |erg11||sterol 14-demethylase|Schizosaccharomyces ...    55   9e-09
SPAC22A12.01c |pso2|snm1, SPAC56F8.17c, snm1|DNA 5' exonuclease ...    30   0.28 
SPAC890.06 |||nucleoporin Nup157/170|Schizosaccharomyces pombe|c...    30   0.37 
SPBC26H8.09c |snf59||SWI/SNF complex subunit Snf59|Schizosacchar...    27   2.0  
SPBC19G7.01c |msh2|swi8, mut3, SPBC24C6.12c|MutS protein homolog...    27   2.0  
SPBC725.13c |psf2|dre13, bsh3|GINS complex subunit Psf2|Schizosa...    27   2.6  
SPAC1F5.11c |||phosphatidylinositol kinase |Schizosaccharomyces ...    27   2.6  
SPBC25H2.11c |||bromodomain protein|Schizosaccharomyces pombe|ch...    27   3.4  
SPCC320.07c |mde7||RNA-binding protein Mde7|Schizosaccharomyces ...    27   3.4  
SPBC15D4.05 |||conserved protein|Schizosaccharomyces pombe|chr 2...    26   4.5  
SPBC947.01 |||AAA family ATPase, unknown biological role|Schizos...    26   4.5  
SPAC23H3.06 |apl6||AP-3 adaptor complex subunit Apl6 |Schizosacc...    26   6.0  
SPAC1D4.14 |tho2|SPAC22F3.14c|THO complex subunit Tho2 |Schizosa...    26   6.0  
SPBC13E7.01 |cwf22|SPBC15D4.16|splicing factor Cwf22|Schizosacch...    26   6.0  
SPCC1322.05c |||leukotriene A-4 hydrolase |Schizosaccharomyces p...    25   7.9  
SPBC336.12c |cdc10||MBF transcription factor complex subunit Cdc...    25   7.9  

>SPAC19A8.04 |erg5||C-22 sterol desaturase Erg5 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 541

 Score = 55.2 bits (127), Expect = 9e-09
 Identities = 51/184 (27%), Positives = 79/184 (42%), Gaps = 14/184 (7%)
 Frame = +3

Query: 87  NKSKSDDEKPVLEKLLDINEEYAYIMASDMLVAGVDTTSNTMSATLYLMAINQDKQQKLR 266
           +++K   EKP +  + + ++E   +     L A  D TS+ M+    L+A + D  QK+R
Sbjct: 288 SENKEGAEKPSV-LIREFSDEEISLTFLSFLFASQDATSSAMTWLFQLLADHPDVLQKVR 346

Query: 267 EEVMS-KNG------------KRSYLRACIKEAMRILPVVSGNMRRTTKEYNIL-GYHIP 404
           EE +  + G            K +Y RA +KE +R+ P V     R  K + I   Y +P
Sbjct: 347 EEQLRIRKGDIDVPLSLDLMEKMTYTRAVVKECLRLRPPVLMVPYRVKKAFPITPDYTVP 406

Query: 405 ENVDIAFAHQHLSMMEKYYPRPTEFIPERWLTNKSDPLYYGNAHPFANSPFGFGVRSCIG 584
           ++  +           K YP P  F P+RW  N           P     FG G   C+G
Sbjct: 407 KDAMVIPTLYGALHDSKVYPEPETFNPDRWAPNG-----LAEQSPKNWMVFGNGPHVCLG 461

Query: 585 RRIA 596
           +R A
Sbjct: 462 QRYA 465


>SPAC13A11.02c |erg11||sterol 14-demethylase|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 495

 Score = 55.2 bits (127), Expect = 9e-09
 Identities = 51/197 (25%), Positives = 91/197 (46%), Gaps = 25/197 (12%)
 Frame = +3

Query: 174 MLVAGVDTTSNTMSATLYLMAINQDKQQKLREE---VMSKNGKRSY--------LRACIK 320
           +L+AG  T++ T+   L L+    +  + L EE   V+ +N +  +        L   I+
Sbjct: 285 LLMAGQHTSAATIVWVLALLGSKPEIIEMLWEEQKRVVGENLELKFDQYKDMPLLNYVIQ 344

Query: 321 EAMRILPVVSGNMRRTTKEYNILGYHI--PENVDIAFAHQHLSMMEKYYPRPTEFIPERW 494
           E +R+ P +  +MR+  ++  + G  I  P N  +  A    +  E+Y+   T+F P+RW
Sbjct: 345 ETLRLHPPIHSHMRKVKRDLPVPGSKIVIPANNYLLAAPGLTATEEEYFTHATDFDPKRW 404

Query: 495 -----LTNKSDPLYY-------GNAHPFANSPFGFGVRSCIGRRIAELEVETFLSKIVEN 638
                    ++ + Y       G A P+   PFG G   CIG + A + + T +SK V +
Sbjct: 405 NDRVNEDENAEQIDYGYGLVTKGAASPYL--PFGAGRHRCIGEQFAYMHLSTIISKFVHD 462

Query: 639 FQVEWSGSSPRVEQTSI 689
           +     G  P V+ +S+
Sbjct: 463 YTWTLIGKVPNVDYSSM 479


>SPAC22A12.01c |pso2|snm1, SPAC56F8.17c, snm1|DNA 5' exonuclease
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 560

 Score = 30.3 bits (65), Expect = 0.28
 Identities = 17/48 (35%), Positives = 27/48 (56%)
 Frame = +3

Query: 318 KEAMRILPVVSGNMRRTTKEYNILGYHIPENVDIAFAHQHLSMMEKYY 461
           K+A+  LP    N+R     Y ++ Y+IP  VD AFA+  +  +E Y+
Sbjct: 166 KQALLPLPGKQINVRSVVPFYKLMPYNIPFAVD-AFAYGAIDGVEAYF 212


>SPAC890.06 |||nucleoporin Nup157/170|Schizosaccharomyces pombe|chr
            1|||Manual
          Length = 1315

 Score = 29.9 bits (64), Expect = 0.37
 Identities = 19/73 (26%), Positives = 40/73 (54%), Gaps = 5/73 (6%)
 Frame = +3

Query: 57   IKKALDDIKMNKSKSDDEKPVLEKLLDINEEYAYIMASDMLVAG--VDTTSNT-MSATLY 227
            +   L ++ +  S+S++  P+ E+++DI E YA+    + +  G  +DT     +S  L 
Sbjct: 1164 VSSTLKNLTLRFSQSENVFPI-EQIIDITERYAFDQQGEAVATGWVIDTFLGAGVSHELI 1222

Query: 228  LMAINQ--DKQQK 260
             + +NQ  D+++K
Sbjct: 1223 FIVLNQLYDRREK 1235


>SPBC26H8.09c |snf59||SWI/SNF complex subunit
           Snf59|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 515

 Score = 27.5 bits (58), Expect = 2.0
 Identities = 15/46 (32%), Positives = 26/46 (56%), Gaps = 2/46 (4%)
 Frame = -2

Query: 670 LGDDPDH--ST*KFSTIFDKNVSTSNSAMRRPIQLRTPKPNGEFAN 539
           + ++P H  S+ K ST  D N +   S  ++  ++  PKP+G+F N
Sbjct: 114 MSEEPKHHDSSNKESTNLD-NSNMDESENQKNFKIEEPKPSGDFRN 158


>SPBC19G7.01c |msh2|swi8, mut3, SPBC24C6.12c|MutS protein homolog
           2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 982

 Score = 27.5 bits (58), Expect = 2.0
 Identities = 21/110 (19%), Positives = 48/110 (43%), Gaps = 4/110 (3%)
 Frame = +3

Query: 78  IKMNKSKSDDEKPVLEKLLDINEEYAYI--MASDMLVAGVDTTSNTMSATLYLMAINQDK 251
           I+ ++  + D +  L K+LD    +A +  +   + +A  +     +   L    +  D+
Sbjct: 237 IRSSEFSARDVELELSKVLDSPVTHALVPELGLQLAMASCNALLRYLGPALLNPDMEDDR 296

Query: 252 QQKLREEVMSKNGKRSYLRACIK--EAMRILPVVSGNMRRTTKEYNILGY 395
           +   R+  +  +    Y+R  I    ++ +LP  +GN  +T   Y +L +
Sbjct: 297 ENTSRKLHLYHHNLEQYMRLDIAAVRSLNLLPPPNGNAHKTMSLYGLLNH 346


>SPBC725.13c |psf2|dre13, bsh3|GINS complex subunit
           Psf2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 183

 Score = 27.1 bits (57), Expect = 2.6
 Identities = 13/43 (30%), Positives = 22/43 (51%)
 Frame = +1

Query: 361 DAPQRSTTFLDIIYRKMSTLHLLINICR*WRNTIQDRRSSFLN 489
           D    + TF ++ +R + T HLL+N C      ++D R   L+
Sbjct: 86  DDELENETFSELPFRWLETAHLLLNFCADDIEDVEDIRRILLD 128


>SPAC1F5.11c |||phosphatidylinositol kinase |Schizosaccharomyces
            pombe|chr 1|||Manual
          Length = 3655

 Score = 27.1 bits (57), Expect = 2.6
 Identities = 30/132 (22%), Positives = 62/132 (46%), Gaps = 3/132 (2%)
 Frame = +3

Query: 126  KLLDINEEYAYIMASDMLVAGVDTTSN--TMSATLYLMAINQDKQQKLREEVMSKNGKRS 299
            +L    EE AY +   +L  G+   S    +S   + + ++Q   Q+  E V+  + + +
Sbjct: 3146 RLKSFPEEDAYRLIVALLNDGLQYISRLGVVSKNTFQLPMSQANIQRFAENVLPVSVREA 3205

Query: 300  YLRACIKEAMRILPVVSGNMRRTTKEY-NILGYHIPENVDIAFAHQHLSMMEKYYPRPTE 476
            +LR  ++  + +L  V   +R   K++ NIL    P+ + +     +LS  +       E
Sbjct: 3206 FLRDFVETKLDLLTYVD-KLRMWRKKFENILDQR-PKFLHLEQCSLYLSEFQHQKFDEVE 3263

Query: 477  FIPERWLTNKSD 512
             IP ++L +K++
Sbjct: 3264 -IPGQYLLDKNN 3274


>SPBC25H2.11c |||bromodomain protein|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 979

 Score = 26.6 bits (56), Expect = 3.4
 Identities = 14/38 (36%), Positives = 24/38 (63%), Gaps = 1/38 (2%)
 Frame = +3

Query: 39  NLTKYFIKKALDDIKMNKSK-SDDEKPVLEKLLDINEE 149
           N T   +K+ L D+K NKSK ++D++   E+L +  E+
Sbjct: 272 NATDADVKQLLSDVKKNKSKWANDQRIGQEELYEAAEK 309


>SPCC320.07c |mde7||RNA-binding protein Mde7|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 761

 Score = 26.6 bits (56), Expect = 3.4
 Identities = 20/89 (22%), Positives = 43/89 (48%), Gaps = 1/89 (1%)
 Frame = +3

Query: 57  IKKALDDIKMNKSKSDDEKPVLEKLLDINEEYAYIMASDMLVAGVDTTSNTMSATLYLMA 236
           + ++   +K    +S +   V E      EE+   +  + + + V T  N+   T+Y+  
Sbjct: 549 VVRSRPSLKQRPLRSAEYVRVCELKCLQEEEFDNKVLLEQVESNVQTDHNSPCNTIYVGN 608

Query: 237 I-NQDKQQKLREEVMSKNGKRSYLRACIK 320
           + N D+++KLR   ++ + ++ Y R C K
Sbjct: 609 LSNPDQEKKLR---LAFSKEKGYRRLCFK 634


>SPBC15D4.05 |||conserved protein|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 411

 Score = 26.2 bits (55), Expect = 4.5
 Identities = 14/52 (26%), Positives = 26/52 (50%)
 Frame = +3

Query: 141 NEEYAYIMASDMLVAGVDTTSNTMSATLYLMAINQDKQQKLREEVMSKNGKR 296
           N+  ++   SD L+   D+T + +  T+    +   K   LR  + ++NGKR
Sbjct: 38  NDIDSFEQQSDFLLVDHDSTLDPVPVTILTGFLGAGKTSLLRSILENRNGKR 89


>SPBC947.01 |||AAA family ATPase, unknown biological
           role|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 660

 Score = 26.2 bits (55), Expect = 4.5
 Identities = 11/30 (36%), Positives = 16/30 (53%)
 Frame = -2

Query: 631 TIFDKNVSTSNSAMRRPIQLRTPKPNGEFA 542
           TI  K +S S +    P+Q  TP  + E+A
Sbjct: 335 TIVSKTISASTTQQTEPLQQTTPSSDFEYA 364


>SPAC23H3.06 |apl6||AP-3 adaptor complex subunit Apl6
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 745

 Score = 25.8 bits (54), Expect = 6.0
 Identities = 11/27 (40%), Positives = 17/27 (62%)
 Frame = -2

Query: 631 TIFDKNVSTSNSAMRRPIQLRTPKPNG 551
           T++DKN  T ++A+R    +R P  NG
Sbjct: 114 TLYDKNPLTRSTAIRVMSSIRVPAING 140


>SPAC1D4.14 |tho2|SPAC22F3.14c|THO complex subunit Tho2
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1628

 Score = 25.8 bits (54), Expect = 6.0
 Identities = 10/28 (35%), Positives = 15/28 (53%)
 Frame = +3

Query: 474 EFIPERWLTNKSDPLYYGNAHPFANSPF 557
           EF+PE WL ++ D  +  N +   N  F
Sbjct: 122 EFLPEEWLLSRLDCKFLENINVVPNGDF 149


>SPBC13E7.01 |cwf22|SPBC15D4.16|splicing factor
           Cwf22|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 834

 Score = 25.8 bits (54), Expect = 6.0
 Identities = 12/41 (29%), Positives = 19/41 (46%)
 Frame = -3

Query: 492 SVQE*TPSVLDSISPSSTNVDEQMQCRHFPVYDIQECCTPL 370
           SV +  P V+D  + S  N+ + +        D +ECC  L
Sbjct: 393 SVNDEKPQVIDQTNASLVNLRKSIYLTIMSSVDFEECCHKL 433


>SPCC1322.05c |||leukotriene A-4 hydrolase |Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 612

 Score = 25.4 bits (53), Expect = 7.9
 Identities = 7/13 (53%), Positives = 11/13 (84%)
 Frame = +2

Query: 188 CRYHFKHDVRNFV 226
           C+Y F+HD+ NF+
Sbjct: 228 CKYEFEHDMENFM 240


>SPBC336.12c |cdc10||MBF transcription factor complex subunit
           Cdc10|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 767

 Score = 25.4 bits (53), Expect = 7.9
 Identities = 13/29 (44%), Positives = 17/29 (58%)
 Frame = +2

Query: 170 RHAGGWCRYHFKHDVRNFVPHGDKSGQTA 256
           +HA G   Y  K D  N++ H DK+G TA
Sbjct: 460 KHASGNNGYMLK-DFINYLNHQDKNGDTA 487


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,993,335
Number of Sequences: 5004
Number of extensions: 66551
Number of successful extensions: 255
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 241
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 254
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 323158234
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)