Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmmt20d15
(726 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC146.07 |prp2|mis11|U2AF large subunit |Schizosaccharomyces p... 36 0.004
SPAC328.05 |||RNA-binding protein involved in export of mRNAs|Sc... 35 0.010
SPBC3B9.11c |ctf1||mRNA cleavage and polyadenylation specificity... 34 0.024
SPBC18H10.04c |sce3|tif48|translation initiation factor eIF4B|Sc... 32 0.073
SPCC1827.05c |||nucleolar RNA-binding protein NIFK |Schizosaccha... 31 0.22
SPAC644.16 |||RNA-binding protein|Schizosaccharomyces pombe|chr ... 31 0.22
SPBC16E9.12c |pab2||poly|Schizosaccharomyces pombe|chr 2|||Manual 29 0.51
SPBC660.15 |||mRNA cleavage factor complex subunit |Schizosaccha... 29 0.68
SPBC839.10 |usp107|snu71|U1 snRNP-associated protein Usp107|Schi... 29 0.89
SPBC887.05c |cwf29||RNA-binding protein Cwf29|Schizosaccharomyce... 28 1.2
SPAC2F7.11 |nrd1|msa2|RNA-binding protein Nrd1|Schizosaccharomyc... 28 1.6
SPBC14C8.04 |||acetolactate synthase regulatory unit|Schizosacch... 28 1.6
SPBC1861.04c |||RNA-binding protein Prp24|Schizosaccharomyces po... 27 2.1
SPAC16.02c |srp2||mRNA export factor Srp2|Schizosaccharomyces po... 27 2.7
SPCC16C4.07 |scw1||RNA-binding protein Scw1|Schizosaccharomyces ... 27 3.6
SPAC1F7.11c |||transcription factor zf-fungal binuclear cluster ... 27 3.6
SPBC13A2.01c |||nuclear cap-binding complex small subunit|Schizo... 26 4.8
SPAC140.02 |gar2||GAR family|Schizosaccharomyces pombe|chr 1|||M... 26 6.3
SPAC1687.20c |mis6||inner centromere protein Mis6|Schizosaccharo... 26 6.3
>SPBC146.07 |prp2|mis11|U2AF large subunit |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 517
Score = 36.3 bits (80), Expect = 0.004
Identities = 36/149 (24%), Positives = 63/149 (42%), Gaps = 16/149 (10%)
Frame = +3
Query: 252 WDVPPPGFEHITPLQYK---------AMQAAGQIPANIV--ADTPQAAV----PVVGSTI 386
WD+ PPG+E +T Q K A +AA P ++ A + + ++ P +
Sbjct: 135 WDIKPPGYELVTADQAKMSGVFPLPGAPRAAVTDPEKLLEFARSAEGSIIAPPPPLQPGA 194
Query: 387 TRQARRLYVGNIPFGVTEEETMEFFNQQMHLSGLAQAAGNPVLACQINLDKNFAFLEFRS 566
+RQARRL V IP E+ + F + + + + ++NFA LE +
Sbjct: 195 SRQARRLVVTGIPNEFVEDAFVSFIEDLFISTTYHKPETKHFSSVNVCKEENFAILEVAT 254
Query: 567 IDETTQAMAFDGINFKGQ-SLKIRRPHDY 650
++ T ++ LK +R +Y
Sbjct: 255 PEDATFLWGLQSESYSNDVFLKFQRIQNY 283
>SPAC328.05 |||RNA-binding protein involved in export of
mRNAs|Schizosaccharomyces pombe|chr 1|||Manual
Length = 434
Score = 35.1 bits (77), Expect = 0.010
Identities = 26/101 (25%), Positives = 42/101 (41%), Gaps = 1/101 (0%)
Frame = +3
Query: 375 GSTITRQARRLYVGNIPFGVTEEETMEFFNQQMHLSGLAQAAGNPVLACQINLDKNFAFL 554
G T+Q RR+YVGN+ + V E EF Q + +L L K A +
Sbjct: 58 GQKYTQQERRVYVGNLSYQVRWFELKEFMGQ------VGNVLNCEILNLPNGLSKGCAII 111
Query: 555 EFRSIDETTQAM-AFDGINFKGQSLKIRRPHDYQPMPGTEN 674
E+ + +E A+ F G+ + IR + G+ +
Sbjct: 112 EYSTAEEARTAIKTLSNQKFMGRLVYIREDREQNARFGSSS 152
Score = 29.9 bits (64), Expect = 0.39
Identities = 19/98 (19%), Positives = 43/98 (43%), Gaps = 1/98 (1%)
Frame = +3
Query: 405 LYVGNIPFGVTEEETMEFFNQQMHLSGLAQAAGNPVLACQINLDKNFAFLEFRSIDETTQ 584
+YVGN+P+ ++ ++ F + A+ A P K F ++F + ++
Sbjct: 282 IYVGNLPWATSDRNLLDLFTDIGSVI-RARIAYEPT-----GRSKGFGVVQFENENDAAS 335
Query: 585 AM-AFDGINFKGQSLKIRRPHDYQPMPGTENPAINVPA 695
++ +G + G+ L++ H P+P +P+
Sbjct: 336 SIEKLNGYRYGGRPLQLSYAHYATPLPAVPTVLSGLPS 373
>SPBC3B9.11c |ctf1||mRNA cleavage and polyadenylation specificity
factor complex subunit Ctf1|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 363
Score = 33.9 bits (74), Expect = 0.024
Identities = 23/68 (33%), Positives = 33/68 (48%)
Frame = +3
Query: 405 LYVGNIPFGVTEEETMEFFNQQMHLSGLAQAAGNPVLACQINLDKNFAFLEFRSIDETTQ 584
++VGNIP+ V+E++ E FNQ + VL + K + F EF D T
Sbjct: 9 VFVGNIPYDVSEQQMTEIFNQVGPVKTF-----KLVLDPETGSGKGYGFCEF--FDSETT 61
Query: 585 AMAFDGIN 608
AMA +N
Sbjct: 62 AMAVRKLN 69
>SPBC18H10.04c |sce3|tif48|translation initiation factor
eIF4B|Schizosaccharomyces pombe|chr 2|||Manual
Length = 388
Score = 32.3 bits (70), Expect = 0.073
Identities = 19/75 (25%), Positives = 37/75 (49%)
Frame = +3
Query: 408 YVGNIPFGVTEEETMEFFNQQMHLSGLAQAAGNPVLACQINLDKNFAFLEFRSIDETTQA 587
+VGN+ F +TE + +FF + + L V+ + F ++EF + D + A
Sbjct: 97 HVGNLSFDLTENDLGDFFGEGVTSIRL-------VIDPLTERSRGFGYVEFETADTLSAA 149
Query: 588 MAFDGINFKGQSLKI 632
+A G + G+ ++I
Sbjct: 150 LALSGEDLMGRPVRI 164
>SPCC1827.05c |||nucleolar RNA-binding protein NIFK
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 276
Score = 30.7 bits (66), Expect = 0.22
Identities = 17/56 (30%), Positives = 29/56 (51%)
Frame = +3
Query: 405 LYVGNIPFGVTEEETMEFFNQQMHLSGLAQAAGNPVLACQINLDKNFAFLEFRSID 572
LYVG +P G E++ +F+Q + L + + K++AF+EF S+D
Sbjct: 107 LYVGRLPHGFYEKQMRMYFSQFGPVLRLRMSRNR-----KTGSSKHYAFIEFESLD 157
>SPAC644.16 |||RNA-binding protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 422
Score = 30.7 bits (66), Expect = 0.22
Identities = 21/68 (30%), Positives = 34/68 (50%)
Frame = +3
Query: 405 LYVGNIPFGVTEEETMEFFNQQMHLSGLAQAAGNPVLACQINLDKNFAFLEFRSIDETTQ 584
+YVGNIP+ + EE+ ++ F Q SG ++ V+ + K + F E+ D T
Sbjct: 7 VYVGNIPYEMAEEQVIDIFKQ----SGPVKSF-QLVIDPESGQPKGYGFCEYH--DPATA 59
Query: 585 AMAFDGIN 608
A A +N
Sbjct: 60 ASAVRNLN 67
>SPBC16E9.12c |pab2||poly|Schizosaccharomyces pombe|chr 2|||Manual
Length = 166
Score = 29.5 bits (63), Expect = 0.51
Identities = 21/80 (26%), Positives = 34/80 (42%), Gaps = 1/80 (1%)
Frame = +3
Query: 396 ARRLYVGNIPFGVTEEETMEFFNQQMHLSGLAQAAGNPVLACQI-NLDKNFAFLEFRSID 572
A+ +YVGN+ + VT EE Q H + +L + K FA++EF
Sbjct: 54 AQSVYVGNVDYSVTPEEL------QSHFASCGSVNRVTILCDKFTGHPKGFAYIEFSEPS 107
Query: 573 ETTQAMAFDGINFKGQSLKI 632
A+ +G + LK+
Sbjct: 108 LVPNALLLNGSMLHERPLKV 127
>SPBC660.15 |||mRNA cleavage factor complex subunit
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 474
Score = 29.1 bits (62), Expect = 0.68
Identities = 11/22 (50%), Positives = 15/22 (68%)
Frame = +3
Query: 402 RLYVGNIPFGVTEEETMEFFNQ 467
+++VG +P TEEE FFNQ
Sbjct: 248 KMFVGGVPGDCTEEEFRNFFNQ 269
>SPBC839.10 |usp107|snu71|U1 snRNP-associated protein
Usp107|Schizosaccharomyces pombe|chr 2|||Manual
Length = 695
Score = 28.7 bits (61), Expect = 0.89
Identities = 25/91 (27%), Positives = 47/91 (51%), Gaps = 1/91 (1%)
Frame = +3
Query: 321 QIPANIVADTPQAAVPVVGSTITRQARRLYVGNIPFGVTEEETMEFF-NQQMHLSGLAQA 497
Q P++ + T + + P V + R L++GNIP +E +F+ ++ + LSG +
Sbjct: 113 QTPSSEKSTTERESRPFVPPNSQQMRRMLFIGNIP-----KELDDFWMDKILRLSG--KL 165
Query: 498 AGNPVLACQINLDKNFAFLEFRSIDETTQAM 590
A +A N +F F EF S ++ ++A+
Sbjct: 166 ASWRRVADADNSMTSFGFAEFESNEQFSRAL 196
>SPBC887.05c |cwf29||RNA-binding protein Cwf29|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 217
Score = 28.3 bits (60), Expect = 1.2
Identities = 26/101 (25%), Positives = 46/101 (45%), Gaps = 3/101 (2%)
Frame = +3
Query: 405 LYVGNIPFGVTEEETMEFFNQQMHLSGLAQAAGNPVLACQINLDKNFAFLEFRSIDETTQ 584
+Y+GN+ F + E++ + F++ + N V + K FAFL++ D+ +
Sbjct: 33 IYIGNLDFDLNEDDILCVFSEFGEPVDI-----NLVRDKETGKSKGFAFLKYE--DQRST 85
Query: 585 AMAFDGI-NFKGQSLKIRRPH--DYQPMPGTENPAINVPAG 698
+A D + N K +R H Y+ + PA VP G
Sbjct: 86 VLAVDNMTNVKLLDRLVRVDHVASYKVPQKEKEPANLVPLG 126
>SPAC2F7.11 |nrd1|msa2|RNA-binding protein Nrd1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 529
Score = 27.9 bits (59), Expect = 1.6
Identities = 25/94 (26%), Positives = 44/94 (46%)
Frame = +3
Query: 399 RRLYVGNIPFGVTEEETMEFFNQQMHLSGLAQAAGNPVLACQINLDKNFAFLEFRSIDET 578
R +++GN+P G+TE+E E + G P+ +I ++N AF+ F +I
Sbjct: 206 RNVFLGNLPNGITEDEIRE----DLEPFG-------PIDQIKIVTERNIAFVHFLNIAAA 254
Query: 579 TQAMAFDGINFKGQSLKIRRPHDYQPMPGTENPA 680
+A+ +N K +I D + G + PA
Sbjct: 255 IKAVQELPLNPKWSKRRIYYGRD-RCAVGLKQPA 287
>SPBC14C8.04 |||acetolactate synthase regulatory
unit|Schizosaccharomyces pombe|chr 2|||Manual
Length = 289
Score = 27.9 bits (59), Expect = 1.6
Identities = 12/34 (35%), Positives = 19/34 (55%)
Frame = +3
Query: 285 TPLQYKAMQAAGQIPANIVADTPQAAVPVVGSTI 386
+P+ YKA+ A +P + + P+A VP S I
Sbjct: 23 SPITYKALHANSPLPRCRIIEPPRATVPEAVSNI 56
>SPBC1861.04c |||RNA-binding protein Prp24|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1014
Score = 27.5 bits (58), Expect = 2.1
Identities = 15/45 (33%), Positives = 20/45 (44%)
Frame = +3
Query: 327 PANIVADTPQAAVPVVGSTITRQARRLYVGNIPFGVTEEETMEFF 461
P + P P G+ + R LYV NI F V E++ FF
Sbjct: 735 PIQVFISDPLRRTPRSGAVY--EGRELYVTNIDFKVNEKDVETFF 777
>SPAC16.02c |srp2||mRNA export factor Srp2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 365
Score = 27.1 bits (57), Expect = 2.7
Identities = 11/20 (55%), Positives = 14/20 (70%)
Frame = +3
Query: 402 RLYVGNIPFGVTEEETMEFF 461
RL+VG IP T E+ M+FF
Sbjct: 5 RLFVGRIPPQATREDMMDFF 24
>SPCC16C4.07 |scw1||RNA-binding protein Scw1|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 561
Score = 26.6 bits (56), Expect = 3.6
Identities = 17/59 (28%), Positives = 26/59 (44%)
Frame = +3
Query: 297 YKAMQAAGQIPANIVADTPQAAVPVVGSTITRQARRLYVGNIPFGVTEEETMEFFNQQM 473
+ A ++ I I A TP P + +YVGN+P +EEE F+ Q+
Sbjct: 395 FSAYSSSHGIHQRIPASTPTNTNPADQNP---PCNTIYVGNLPPSTSEEELKVLFSTQV 450
>SPAC1F7.11c |||transcription factor zf-fungal binuclear cluster
type |Schizosaccharomyces pombe|chr 1|||Manual
Length = 782
Score = 26.6 bits (56), Expect = 3.6
Identities = 11/26 (42%), Positives = 12/26 (46%)
Frame = +1
Query: 361 PCRWWVRL*HVKREGYMSATYPLASP 438
P RWWV H G + A Y L P
Sbjct: 550 PLRWWVFRFHSLNAGIIQAAYALCFP 575
>SPBC13A2.01c |||nuclear cap-binding complex small
subunit|Schizosaccharomyces pombe|chr 2|||Manual
Length = 182
Score = 26.2 bits (55), Expect = 4.8
Identities = 10/26 (38%), Positives = 17/26 (65%)
Frame = +3
Query: 390 RQARRLYVGNIPFGVTEEETMEFFNQ 467
+Q+ +YVGN+ F TEE+ F++
Sbjct: 29 KQSNCVYVGNLSFYTTEEQIYALFSK 54
>SPAC140.02 |gar2||GAR family|Schizosaccharomyces pombe|chr
1|||Manual
Length = 500
Score = 25.8 bits (54), Expect = 6.3
Identities = 19/86 (22%), Positives = 36/86 (41%)
Frame = +3
Query: 375 GSTITRQARRLYVGNIPFGVTEEETMEFFNQQMHLSGLAQAAGNPVLACQINLDKNFAFL 554
G ++ + ++VGN+ F TE++ F G Q+ P Q K F ++
Sbjct: 358 GDQLSEPSDTVFVGNLSFNATEDDLSTAFGG----CGDIQSIRLPT-DPQSGRLKGFGYV 412
Query: 555 EFRSIDETTQAMAFDGINFKGQSLKI 632
F ID + + +G G+ ++
Sbjct: 413 TFSDIDSAKKCVEMNGHFIAGRPCRL 438
>SPAC1687.20c |mis6||inner centromere protein
Mis6|Schizosaccharomyces pombe|chr 1|||Manual
Length = 672
Score = 25.8 bits (54), Expect = 6.3
Identities = 15/44 (34%), Positives = 19/44 (43%)
Frame = +3
Query: 351 PQAAVPVVGSTITRQARRLYVGNIPFGVTEEETMEFFNQQMHLS 482
PQ + G I Q G+IP +EET F +HLS
Sbjct: 597 PQFFSYINGYQIYLQQTNPATGSIPLKPIQEETFGAFQSNLHLS 640
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,757,163
Number of Sequences: 5004
Number of extensions: 58317
Number of successful extensions: 182
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 173
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 180
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 341222980
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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