Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= bmmt20b11
         (630 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC1711.08 |||chaperone activator Aha1 |Schizosaccharomyces pom...    59   6e-10
SPAC589.05c |||conserved eukaryotic protein|Schizosaccharomyces ...    27   2.2  
SPBC215.15 |sec13||COPII-coated vesicle component Sec13|Schizosa...    25   6.8  
SPBC336.06c |rnh1||ribonuclease H Rnh1|Schizosaccharomyces pombe...    25   6.8  
SPAC1002.21 |||sequence orphan|Schizosaccharomyces pombe|chr 1||...    25   9.0  
SPBC336.14c |ppk26||serine/threonine protein kinase Ppk26|Schizo...    25   9.0  

>SPBC1711.08 |||chaperone activator Aha1 |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 336

 Score = 58.8 bits (136), Expect = 6e-10
 Identities = 30/106 (28%), Positives = 52/106 (49%)
 Frame = +3

Query: 309 NVNNWHWTEKNAGPWSKDRLKELFSDLKIAQNGIVCSITEVEKVDGEASANNRKGKLIFF 488
           N NNWHWT K+   WS +   +    ++ ++      IT+V   +G+   + RK K+I  
Sbjct: 7   NPNNWHWTSKDCRVWSHEYFNKELPKIQASEGPTSARITQVNSCEGDVDVSMRKRKVITI 66

Query: 489 YEWDIKLKWEGVLAGGSEKIKGEIHIPNLSEENDVSEVDMTVTIKS 626
           ++  I+++++G    G E   G I  P LS +   S+    + I S
Sbjct: 67  FDLKIQMEFKGETKDGVE-ATGSITCPELSYDLGYSDYVFDIDIYS 111


>SPAC589.05c |||conserved eukaryotic protein|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 346

 Score = 27.1 bits (57), Expect = 2.2
 Identities = 18/62 (29%), Positives = 28/62 (45%), Gaps = 6/62 (9%)
 Frame = +3

Query: 351 WSKDRLKELFSDLKIAQNGIV------CSITEVEKVDGEASANNRKGKLIFFYEWDIKLK 512
           +S D  K L  +  IA N  +      CSI  VEK+    +  +     I F+ WD+  +
Sbjct: 260 YSSDFKKRLLKNELIAHNDPMEIEMRGCSIWAVEKILQNINRKDVNAITIDFFLWDLAKE 319

Query: 513 WE 518
           W+
Sbjct: 320 WQ 321


>SPBC215.15 |sec13||COPII-coated vesicle component
           Sec13|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 297

 Score = 25.4 bits (53), Expect = 6.8
 Identities = 11/26 (42%), Positives = 16/26 (61%), Gaps = 1/26 (3%)
 Frame = +3

Query: 315 NNWHWTEKNAGPWSKDRL-KELFSDL 389
           N + WT++  GPW K  L +E F D+
Sbjct: 232 NVFIWTKEGDGPWQKTPLTEEKFPDI 257


>SPBC336.06c |rnh1||ribonuclease H Rnh1|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 264

 Score = 25.4 bits (53), Expect = 6.8
 Identities = 14/49 (28%), Positives = 26/49 (53%)
 Frame = +3

Query: 327 WTEKNAGPWSKDRLKELFSDLKIAQNGIVCSITEVEKVDGEASANNRKG 473
           + +K +  +S ++  E+FS+    +  I CS  +V   DG +  N +KG
Sbjct: 92  YRKKISRSYSTEKDIEIFSN-DTHEKSIACSDRQVVYADGSSLRNGKKG 139


>SPAC1002.21 |||sequence orphan|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 148

 Score = 25.0 bits (52), Expect = 9.0
 Identities = 9/20 (45%), Positives = 12/20 (60%)
 Frame = +3

Query: 270 DPRWIVEERPDATNVNNWHW 329
           D    +EE+    NVNNW+W
Sbjct: 85  DASHCLEEKKFFCNVNNWNW 104


>SPBC336.14c |ppk26||serine/threonine protein kinase
           Ppk26|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 589

 Score = 25.0 bits (52), Expect = 9.0
 Identities = 9/49 (18%), Positives = 27/49 (55%)
 Frame = -3

Query: 388 KSLKSSFNRSFDHGPAFFSVQCQLFTFVASGRSSTIHRGSPSPHLAMVY 242
           + ++   + +F HG  FF++ C++   + + R+S  +  +    ++++Y
Sbjct: 472 EKMERKISDAFQHG-RFFNILCKIMFIIDNNRASREYPIAREKEISLIY 519


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,633,349
Number of Sequences: 5004
Number of extensions: 54942
Number of successful extensions: 132
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 129
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 132
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 279695522
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)