Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= bmmt20a19
         (735 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPCC191.02c ||SPCC417.14c|acetyl-CoA ligase |Schizosaccharomyces...    71   1e-13
SPCC1827.03c |||acetyl-CoA ligase |Schizosaccharomyces pombe|chr...    68   1e-12
SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||...    36   0.006
SPAC19A8.08 |upf2||nonsense-mediated decay protein Upf2|Schizosa...    25   8.5  

>SPCC191.02c ||SPCC417.14c|acetyl-CoA ligase |Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 662

 Score = 71.3 bits (167), Expect = 1e-13
 Identities = 41/110 (37%), Positives = 64/110 (58%), Gaps = 3/110 (2%)
 Frame = +3

Query: 6   YIVDRIKELIKYKAGQVAPSELEAILLQHDAVQDVGVAGAPDPLVGELPTAFVVKKPNSK 185
           +I  R+ +++     +++ +E+EA LL HDAV +  V G  D L G+   AF++ KP  +
Sbjct: 521 WIRGRVDDVVNISGHRLSTAEIEAALLSHDAVAESAVVGVHDELTGQAVNAFILLKPGYE 580

Query: 186 VT---EKELIDFVAARVSSWKQLRGGVRFVNEIPKTGSGKILRRILRDSL 326
            T   EKELI  V + +  +   R  +   +++PKT SGKI+RRILR  L
Sbjct: 581 ATVELEKELIMAVRSTIGPFASPRKLI--FSDLPKTRSGKIMRRILRKIL 628


>SPCC1827.03c |||acetyl-CoA ligase |Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 512

 Score = 68.1 bits (159), Expect = 1e-12
 Identities = 36/115 (31%), Positives = 64/115 (55%)
 Frame = +3

Query: 6   YIVDRIKELIKYKAGQVAPSELEAILLQHDAVQDVGVAGAPDPLVGELPTAFVVKKPNSK 185
           +I  RIKEL+     +++P+E++A+L+QH  V +      PD   G+   A +       
Sbjct: 399 FITGRIKELVNRGGEKISPAEIDAVLMQHPDVSEAVCFAVPDEKYGQDIQAAINPVAGKT 458

Query: 186 VTEKELIDFVAARVSSWKQLRGGVRFVNEIPKTGSGKILRRILRDSLTKPPASKL 350
           VT K+L D++  +V+++K +     F + IPKT +GK+ RR++ D+      +KL
Sbjct: 459 VTPKQLHDYLEQKVAAFK-IPKKFYFTDRIPKTATGKVQRRLVCDAFFNHSKAKL 512


>SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 534

 Score = 35.9 bits (79), Expect = 0.006
 Identities = 29/105 (27%), Positives = 53/105 (50%), Gaps = 3/105 (2%)
 Frame = -3

Query: 370 ASSSKDQSLLAGGLVNESRSMRRNIFPLPVFG---ISFTNLTPPRNCFHEETLAATKSIN 200
           +SSS   S  +  L + S S   +    P      IS ++ +P  + F   T +++KS +
Sbjct: 308 SSSSSSSSSFSSTLSSSSMSSSSSFSSSPTSSSSTISSSSSSPSSSSF-SSTTSSSKSSS 366

Query: 199 SFSVTLEFGFLTTNAVGNSPTRGSGAPATPTS*TASCCNNIASSS 65
           SFS T+     T+++   S +  S  PA+ +S ++S  ++ +SSS
Sbjct: 367 SFSSTVSSSSSTSSSTLTSSSSSSSRPASSSSHSSSLSSHKSSSS 411


>SPAC19A8.08 |upf2||nonsense-mediated decay protein
           Upf2|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1049

 Score = 25.4 bits (53), Expect = 8.5
 Identities = 10/19 (52%), Positives = 13/19 (68%)
 Frame = +3

Query: 468 FQTVLLYLRFDWWKVYQVL 524
           F T+LL  +FDW   YQ+L
Sbjct: 665 FPTLLLLRKFDWKDDYQIL 683


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,040,174
Number of Sequences: 5004
Number of extensions: 64900
Number of successful extensions: 157
Number of sequences better than 10.0: 4
Number of HSP's better than 10.0 without gapping: 152
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 155
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 347244562
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)