Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmmt1o10
(622 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC1773.05c |tms1||hexitol dehydrogenase |Schizosaccharomyces p... 199 2e-52
SPBC1198.01 |||glutathione-dependent formaldehyde dehydrogenase ... 85 1e-17
SPCC13B11.01 |adh1|adh|alcohol dehydrogenase Adh1|Schizosaccharo... 81 1e-16
SPCC13B11.04c ||SPCC777.01c|glutathione-dependent formaldehyde d... 56 3e-09
SPBC1539.07c |||glutathione-dependent formaldehyde dehydrogenase... 55 1e-08
SPBC1773.06c |||alcohol dehydrogenase |Schizosaccharomyces pombe... 29 0.72
SPAC27E2.06c |||methionine-tRNA ligase, mitochondrial|Schizosacc... 27 2.2
SPCC962.06c |bpb1|sf1|zinc finger protein Bpb1|Schizosaccharomyc... 25 6.7
SPBC36B7.09 |gcn2|ppk28, ppk28, SPBP18G5.01|eIF2 alpha kinase Gc... 25 6.7
SPBC6B1.07 |prp1|zer1|U4/U6 x U5 tri-snRNP complex subunit Prp1|... 25 8.8
>SPBC1773.05c |tms1||hexitol dehydrogenase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 360
Score = 199 bits (486), Expect = 2e-52
Identities = 90/191 (47%), Positives = 125/191 (65%), Gaps = 1/191 (0%)
Frame = +1
Query: 43 MATDNLTALLYKPNDLRLVQTPIPEISED-EVLLRMDCVGICGSDVHYWQKGQCGHFVLE 219
MA +L K D + P +++D +V + + GICGSDVHYW++G G F+L+
Sbjct: 1 MAPAEKAFVLRKKMDTAIEDRPGQTLTDDHQVKVAIKATGICGSDVHYWKEGGIGDFILK 60
Query: 220 EPMIMGHEASGVVAKIGSKVKNLTVGDRVAIEPGVPCRYCEFCKTGRYHLCPDMIFCATP 399
+PMI+GHE++GVV ++G V +L GD VA+EPG CR C++C++GRY+LCP M F ATP
Sbjct: 61 KPMILGHESAGVVVEVGKGVSSLKPGDPVAVEPGCVCRLCDYCRSGRYNLCPHMEFAATP 120
Query: 400 PVHGNLVRYYKHAADFCFKLPDHVTMEXGALLEPLAVGIHACXRGGVSAGHVVLVLGAGP 579
P G L YY DFC KLP +++E GAL EP++V +HA RG + G VLV+G G
Sbjct: 121 PYDGTLRTYYITTEDFCTKLPKQISVEEGALFEPMSVAVHAMTRGNLKCGSRVLVMGCGT 180
Query: 580 IGLLTMLTAKS 612
+GLL M AK+
Sbjct: 181 VGLLMMAVAKA 191
>SPBC1198.01 |||glutathione-dependent formaldehyde dehydrogenase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 423
Score = 84.6 bits (200), Expect = 1e-17
Identities = 61/194 (31%), Positives = 87/194 (44%), Gaps = 24/194 (12%)
Frame = +1
Query: 79 PNDLRLVQTPIPEISED-EVLLRMDCVGIC-GSDVHYWQKGQCGHFVLEEPMIMGHEASG 252
P ++++ + P P I+ +V+++ IC GSD H + G +E+ I+GHE+ G
Sbjct: 45 PLNVKIAEVPKPTITHPKDVIVKTTACTICSGSDSHIFSGEMPG---IEKGAILGHESCG 101
Query: 253 VVAKIGSKVKNLTVGDRVAIEPGVPCRYCEFCKTGRYHLCPDMIFCATPPV-----HGNL 417
+VA+ G +V NL +GDRV I + C C FCK Y C V H +
Sbjct: 102 IVAEKGDEVNNLEIGDRVVIAFDLACGQCSFCKRHEYAACDTTNDSKLMDVNYGSHHSAI 161
Query: 418 VRYYKHAADF----------------CFKLPDHVTMEXGALL-EPLAVGIHACXRGGVSA 546
Y K D C KLPD + G + + L +HAC G V
Sbjct: 162 FGYTKLLGDVPGCQAEYIRVPFAEINCCKLPDDIPDSEGLFMSDVLCTSLHACTLGEVKK 221
Query: 547 GHVVLVLGAGPIGL 588
G V + G GPIGL
Sbjct: 222 GDTVAIWGMGPIGL 235
>SPCC13B11.01 |adh1|adh|alcohol dehydrogenase
Adh1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 350
Score = 81.0 bits (191), Expect = 1e-16
Identities = 55/184 (29%), Positives = 90/184 (48%), Gaps = 5/184 (2%)
Frame = +1
Query: 79 PNDLRLVQTPIPEISEDEVLLRMDCVGICGSDVHYWQKGQCGHFVL--EEPMIMGHEASG 252
P +++ + P+ E +DEVL+ + G+C +D+H Q G + L + P+I GHE +G
Sbjct: 18 PENVKFEEVPVAEPGQDEVLVNIKYTGVCHTDLHALQ----GDWPLPAKMPLIGGHEGAG 73
Query: 253 VVAKIGSKVKNLTVGDRVAIE-PGVPCRYCEFCKTGRYHLCPDMIFCATPPVHGNLVRYY 429
VV K+G+ V L +GDRV ++ C CE+C +CP I + V G Y
Sbjct: 74 VVVKVGAGVTRLKIGDRVGVKWMNSSCGNCEYCMKAEETICPH-IQLSGYTVDGTFQHYC 132
Query: 430 KHAADFCFKLPDHVTMEXGALLEPLAVGIH-ACXRGGVSAGHVVLVLGA-GPIGLLTMLT 603
A +P+ V +E A + + + A V G + + GA G +G L +
Sbjct: 133 IANATHATIIPESVPLEVAAPIMCAGITCYRALKESKVGPGEWICIPGAGGGLGHLAVQY 192
Query: 604 AKSL 615
AK++
Sbjct: 193 AKAM 196
>SPCC13B11.04c ||SPCC777.01c|glutathione-dependent formaldehyde
dehydrogenase|Schizosaccharomyces pombe|chr 3|||Manual
Length = 380
Score = 56.4 bits (130), Expect = 3e-09
Identities = 32/98 (32%), Positives = 53/98 (54%)
Frame = +1
Query: 79 PNDLRLVQTPIPEISEDEVLLRMDCVGICGSDVHYWQKGQCGHFVLEEPMIMGHEASGVV 258
P + VQ P + E V +++ G+C +D Y G+ + P+I+GHE +G+V
Sbjct: 24 PLSIENVQVFPPRVHE--VRIKIVNSGVCHTDA-YTLSGKDPEGLF--PVILGHEGAGIV 78
Query: 259 AKIGSKVKNLTVGDRVAIEPGVPCRYCEFCKTGRYHLC 372
+G +V + VGD V C+ C+FCK+G+ +LC
Sbjct: 79 ESVGPQVTTVQVGDPVIALYTPECKTCKFCKSGKTNLC 116
>SPBC1539.07c |||glutathione-dependent formaldehyde dehydrogenase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 378
Score = 54.8 bits (126), Expect = 1e-08
Identities = 27/81 (33%), Positives = 45/81 (55%)
Frame = +1
Query: 130 EVLLRMDCVGICGSDVHYWQKGQCGHFVLEEPMIMGHEASGVVAKIGSKVKNLTVGDRVA 309
EV +++D +C +D Y G P+++GHE +G+V IG V N+ GD V
Sbjct: 36 EVRVKVDWSAVCHTDA-YTLSGVDPEGAF--PIVLGHEGAGIVESIGEGVINVRPGDHVI 92
Query: 310 IEPGVPCRYCEFCKTGRYHLC 372
+ C+ C+FC++G+ +LC
Sbjct: 93 LLYTPECKECKFCRSGKTNLC 113
>SPBC1773.06c |||alcohol dehydrogenase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 346
Score = 28.7 bits (61), Expect = 0.72
Identities = 10/31 (32%), Positives = 19/31 (61%)
Frame = +1
Query: 214 LEEPMIMGHEASGVVAKIGSKVKNLTVGDRV 306
L+ P++ G + +G++ K+G V+ GD V
Sbjct: 58 LQLPVVPGSDGAGIIEKVGEDVEGFEKGDSV 88
>SPAC27E2.06c |||methionine-tRNA ligase,
mitochondrial|Schizosaccharomyces pombe|chr 1|||Manual
Length = 539
Score = 27.1 bits (57), Expect = 2.2
Identities = 21/74 (28%), Positives = 32/74 (43%), Gaps = 1/74 (1%)
Frame = -2
Query: 615 KAFSCEHGKEAD-GSRSQYQHHVSRRDSAPXARVNAHREGLQ*RSXFHGHVIRKLEAKIS 439
K S E GKE S Y +S+ S N + +Q S FH V+ +L+ IS
Sbjct: 168 KRVSMETGKEVQWSSEMNYHFLLSKFQSRLIEHYNKNPNFVQ-PSIFHTQVLEELKTGIS 226
Query: 438 CVLVISDEVSVYWG 397
+ + + + WG
Sbjct: 227 DLSISRPKQRLSWG 240
>SPCC962.06c |bpb1|sf1|zinc finger protein Bpb1|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 587
Score = 25.4 bits (53), Expect = 6.7
Identities = 11/31 (35%), Positives = 17/31 (54%)
Frame = -2
Query: 474 GHVIRKLEAKISCVLVISDEVSVYWGRGAED 382
GH ++ +EAK + I + SV G+G D
Sbjct: 211 GHTLKDMEAKSGAKIAIRGKGSVKEGKGRSD 241
>SPBC36B7.09 |gcn2|ppk28, ppk28, SPBP18G5.01|eIF2 alpha kinase Gcn2
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1576
Score = 25.4 bits (53), Expect = 6.7
Identities = 11/20 (55%), Positives = 14/20 (70%)
Frame = +1
Query: 94 LVQTPIPEISEDEVLLRMDC 153
LVQ+ I E SED + L +DC
Sbjct: 1489 LVQSAIRESSEDAIALAVDC 1508
>SPBC6B1.07 |prp1|zer1|U4/U6 x U5 tri-snRNP complex subunit
Prp1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 906
Score = 25.0 bits (52), Expect = 8.8
Identities = 6/32 (18%), Positives = 13/32 (40%)
Frame = +2
Query: 476 WXKERYWSPSRWAFTRAXGAESRRDTWCWYWE 571
W +++ W + D WCW+++
Sbjct: 821 WLEKKADKARSWFLKAVKADQDNGDVWCWFYK 852
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,558,262
Number of Sequences: 5004
Number of extensions: 50919
Number of successful extensions: 145
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 132
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 143
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 273658928
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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