Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmmt1k21
(748 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC18H10.02 |lcf1||long-chain-fatty-acid-CoA ligase Lcf1 |Schiz... 54 2e-08
SPCC191.02c ||SPCC417.14c|acetyl-CoA ligase |Schizosaccharomyces... 45 1e-05
SPCC1827.03c |||acetyl-CoA ligase |Schizosaccharomyces pombe|chr... 44 2e-05
SPAP7G5.04c |lys1||aminoadipate-semialdehyde dehydrogenase |Schi... 39 9e-04
SPAC23G3.02c |sib1||ferrichrome synthetase Sib1|Schizosaccharomy... 36 0.005
SPBP4H10.11c |||long-chain-fatty-acid-CoA ligase |Schizosaccharo... 35 0.014
SPBC17F3.02 |nak1|orb3, mor4|PAK-related kinase Nak1|Schizosacch... 31 0.17
SPBC337.03 |||conserved eukaryotic protein|Schizosaccharomyces p... 31 0.23
SPCC18B5.03 |wee1||dual specificity protein kinase Wee1|Schizosa... 29 0.53
SPBC947.09 |||ThiJ domain protein|Schizosaccharomyces pombe|chr ... 26 5.0
SPCC584.15c |||arrestin/PY protein 2|Schizosaccharomyces pombe|c... 26 5.0
SPAC4D7.03 |pop2|sud1|F-box/WD repeat protein Pop2|Schizosacchar... 25 8.7
SPCC74.01 |sly1||SNARE binding protein Sly1|Schizosaccharomyces ... 25 8.7
>SPBC18H10.02 |lcf1||long-chain-fatty-acid-CoA ligase Lcf1
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 676
Score = 54.0 bits (124), Expect = 2e-08
Identities = 40/171 (23%), Positives = 84/171 (49%), Gaps = 6/171 (3%)
Frame = +3
Query: 252 MTYGEIVQQSMRFAISLSRLGVRKGERIAILSENRQEFWTAVIGPACVGAIVTTISTNYV 431
+++ +I +++R+A +L +LG+ KG++ + + FW + AC+ +T ++
Sbjct: 97 LSFNDIYDKALRYAGALRKLGLNKGDKFELYAPT-SAFWL-LTAEACLSQSMTIVTAYDT 154
Query: 432 KGE--LKHAMNISKPKYVIISPKMHERHVRTLKSFGFIEKTIIFGDAGSKSGLYSFDE-- 599
GE L H++ S + + + + V LK +E II+ + + + + E
Sbjct: 155 LGEEGLLHSLRESGVRGMYTEGHLLKTLVNPLKEIESLE-VIIYRNDAKEEDIKTIQEIR 213
Query: 600 --LVLAGGGSEIEIEKFTAVNVNGQNDVLFMVYSSGTTGLPKGVLLTHYNV 746
L L ++ + ++ ++Y+SG+TGLPKGV+L+H N+
Sbjct: 214 PNLKLIKFADFEKMSPPVEPDPPSPEEICCIMYTSGSTGLPKGVILSHKNM 264
>SPCC191.02c ||SPCC417.14c|acetyl-CoA ligase |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 662
Score = 44.8 bits (101), Expect = 1e-05
Identities = 41/171 (23%), Positives = 77/171 (45%), Gaps = 9/171 (5%)
Frame = +3
Query: 243 GETMTYGEIVQQSMRFAISLSRLGVRKGERIAILSENRQEFWTAVIGPACVGAIVTTIST 422
G +TY E++ + A +L +GV G+R+AI E A++ +GAI + I
Sbjct: 115 GRYITYRELLASVSQCAGALQSMGVGMGDRVAIYMPMIPETIIAMLAIVRLGAIHSVIFA 174
Query: 423 NYVKGELKHAMNISKPKYVIISPKMHERHVRTLKSFGFIEKTIIFGDAGSKSGLY--SFD 596
+ + +N S+ K +I + + H R + + G + K + K ++ S +
Sbjct: 175 GFSAESVADRVNDSECKVIITADESH-RGGKRIPLKGVVNKALTECPTIKKVLVFQRSAE 233
Query: 597 ELVLAGGGSEI-------EIEKFTAVNVNGQNDVLFMVYSSGTTGLPKGVL 728
G ++ + ++ V LF++Y+SG+TG PKGV+
Sbjct: 234 PTASMVEGRDVWWHDIIPKFPRYCPPAVVNPEHPLFLLYTSGSTGKPKGVV 284
>SPCC1827.03c |||acetyl-CoA ligase |Schizosaccharomyces pombe|chr
3|||Manual
Length = 512
Score = 44.4 bits (100), Expect = 2e-05
Identities = 40/168 (23%), Positives = 76/168 (45%), Gaps = 3/168 (1%)
Frame = +3
Query: 252 MTYGEIVQQSMRFAISLSRLGVRKGERIAILSENRQEFWTAVIGPACVGAIVTTISTNYV 431
+++ E+ M ++ LG++ G+ + I N EF A + AI +++NY
Sbjct: 28 LSFSELRIAIMDLQRQIASLGIKVGDPVNIAIPNGLEFVVAFYAVSWQRAICGPLNSNYK 87
Query: 432 KGELKHAMNISKPKYVII---SPKMHERHVRTLKSFGFIEKTIIFGDAGSKSGLYSFDEL 602
+ E + ++ K K VI+ S + VR K + + + F+
Sbjct: 88 QSEFEFYIDDLKSKLVIVPEGSVAANTPAVRAAKKLSVAVAELAWCPKSRLVRIVHFE-- 145
Query: 603 VLAGGGSEIEIEKFTAVNVNGQNDVLFMVYSSGTTGLPKGVLLTHYNV 746
G++I + + + +DV+ ++++SGTTG PK V LTH N+
Sbjct: 146 -----GAKINAPQ--PLGLPQPDDVMLVLHTSGTTGRPKVVPLTHKNL 186
>SPAP7G5.04c |lys1||aminoadipate-semialdehyde dehydrogenase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1419
Score = 38.7 bits (86), Expect = 9e-04
Identities = 37/171 (21%), Positives = 77/171 (45%), Gaps = 7/171 (4%)
Frame = +3
Query: 255 TYGEIVQQSMRFAISLSRLGVRKGERIAILSENRQEFWTAVIGPACVGAIVTTISTNYVK 434
TY +I + S A L + G+ +G+ + + + + AV+G GA + I Y
Sbjct: 314 TYRQIDESSNILAHHLVKNGIERGDVVMVYAYRGVDLVVAVMGVLKAGATFSVIDPAYPP 373
Query: 435 GELKHAMNISKPKYVII-------SPKMHERHVRTLKSFGFIEKTIIFGDAGSKSGLYSF 593
++++KP+ +++ SP + E ++L+ ++ + D G S
Sbjct: 374 ARQIIYLSVAKPRALVVLEDAGVLSPTVVEYVEKSLELKTYVPALKLAKDGSLTGGSVSK 433
Query: 594 DELVLAGGGSEIEIEKFTAVNVNGQNDVLFMVYSSGTTGLPKGVLLTHYNV 746
+ ++ E+ T V V G + + ++SG+ G+PKGV H+++
Sbjct: 434 GADDILQHVLHLKSEQ-TGVVV-GPDSTPTLSFTSGSEGIPKGVKGRHFSL 482
>SPAC23G3.02c |sib1||ferrichrome synthetase Sib1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 4924
Score = 36.3 bits (80), Expect = 0.005
Identities = 13/26 (50%), Positives = 22/26 (84%)
Frame = +3
Query: 669 NDVLFMVYSSGTTGLPKGVLLTHYNV 746
+++ +++Y+SG+TG PKG LTH+NV
Sbjct: 2870 SNLAYVLYTSGSTGKPKGCCLTHHNV 2895
Score = 31.5 bits (68), Expect = 0.13
Identities = 11/23 (47%), Positives = 19/23 (82%)
Frame = +3
Query: 669 NDVLFMVYSSGTTGLPKGVLLTH 737
+ V +++Y+SG+TG PKGV ++H
Sbjct: 359 DSVAYVLYTSGSTGNPKGVAISH 381
>SPBP4H10.11c |||long-chain-fatty-acid-CoA ligase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 689
Score = 34.7 bits (76), Expect = 0.014
Identities = 13/26 (50%), Positives = 20/26 (76%)
Frame = +3
Query: 669 NDVLFMVYSSGTTGLPKGVLLTHYNV 746
+D+ +Y+SG+TG PKGV+L H N+
Sbjct: 245 DDICCYMYTSGSTGKPKGVVLLHRNI 270
>SPBC17F3.02 |nak1|orb3, mor4|PAK-related kinase
Nak1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 652
Score = 31.1 bits (67), Expect = 0.17
Identities = 21/45 (46%), Positives = 25/45 (55%), Gaps = 2/45 (4%)
Frame = -1
Query: 646 VNFSISISDPPPANTNSSNEYKPLFDPAS--PKIIVFSMNPNDFN 518
VN SISI PPP + + S +KP + AS PKI S P FN
Sbjct: 528 VNRSISI--PPPKSISPSILHKPSLESASSTPKISSCSSTPKPFN 570
>SPBC337.03 |||conserved eukaryotic protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 387
Score = 30.7 bits (66), Expect = 0.23
Identities = 14/46 (30%), Positives = 22/46 (47%)
Frame = -1
Query: 688 TMNKTSFCPFTLTAVNFSISISDPPPANTNSSNEYKPLFDPASPKI 551
T+ F + TA ++ + PP ++ NS + Y P D SP I
Sbjct: 261 TLTDAGFGDKSSTAGKHNVETTSPPSSSPNSDDAYSPQVDSYSPSI 306
>SPCC18B5.03 |wee1||dual specificity protein kinase
Wee1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 877
Score = 29.5 bits (63), Expect = 0.53
Identities = 13/28 (46%), Positives = 19/28 (67%)
Frame = -1
Query: 673 SFCPFTLTAVNFSISISDPPPANTNSSN 590
SF PF ++ NF +S S P P N+N++N
Sbjct: 221 SFSPFATSSPNF-LSTSTPAPPNSNNAN 247
>SPBC947.09 |||ThiJ domain protein|Schizosaccharomyces pombe|chr
2|||Manual
Length = 261
Score = 26.2 bits (55), Expect = 5.0
Identities = 15/44 (34%), Positives = 18/44 (40%)
Frame = +1
Query: 121 ILTTCTGPGTSPFRCT*TSASSYWTECGCTKTGLHRQMEPQAKL 252
+ C GP PF TS S CG T R E ++KL
Sbjct: 158 VAAVCHGPTLLPFMKRQTSDGSVSIVCGKDVTAFDRVAEDKSKL 201
>SPCC584.15c |||arrestin/PY protein 2|Schizosaccharomyces pombe|chr
3|||Manual
Length = 594
Score = 26.2 bits (55), Expect = 5.0
Identities = 19/61 (31%), Positives = 29/61 (47%), Gaps = 1/61 (1%)
Frame = -1
Query: 442 SSPLT*LVLIVVTMAPTHAGPITAVQNSCLFSDSIAILSPLRTP-SRESEIANLIDCCTI 266
+SPL + A T A ++V NS L +LSPL +P S S + + CT+
Sbjct: 440 TSPLAPFSGVTRRAARTRANSASSVFNSQLQPLQTDLLSPLPSPTSSNSRLPRVRSACTL 499
Query: 265 S 263
+
Sbjct: 500 N 500
>SPAC4D7.03 |pop2|sud1|F-box/WD repeat protein
Pop2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 703
Score = 25.4 bits (53), Expect = 8.7
Identities = 12/31 (38%), Positives = 19/31 (61%), Gaps = 4/31 (12%)
Frame = +3
Query: 159 PLHLNFG----QFILDRMWMHKDRIAQTNGA 239
P HL+F F++ + +HKDRI T+G+
Sbjct: 340 PSHLSFPIHVPNFMITSLLLHKDRIITTSGS 370
>SPCC74.01 |sly1||SNARE binding protein Sly1|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 639
Score = 25.4 bits (53), Expect = 8.7
Identities = 15/52 (28%), Positives = 22/52 (42%), Gaps = 4/52 (7%)
Frame = -1
Query: 631 SISDPPPANTNSSNEYKPLFDPASPKIIVFSMNPNDFNVRTCLS----CIFG 488
S+ DP + + E L DP S + I + +P N R S C+ G
Sbjct: 544 SLMDPGSSPASKQTESYLLLDPKSARAITVNNDPRAMNKRQTFSEAIVCVLG 595
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,079,082
Number of Sequences: 5004
Number of extensions: 63871
Number of successful extensions: 195
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 183
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 193
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 355273338
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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