Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= bmmt1j14
         (724 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC22H12.03 |||mitochondrial hydrolase|Schizosaccharomyces pomb...    41   2e-04
SPBP4H10.17c |||carboxyl methyl esterase|Schizosaccharomyces pom...    33   0.041
SPAC6G10.03c |||abhydrolase family protein, unknown biological r...    28   1.6  
SPBC1105.06 |pmc4|med4|RNA polymerase II holoenzyme component Pm...    27   3.6  
SPBC3B8.06 |||conserved fungal protein|Schizosaccharomyces pombe...    26   4.7  
SPAC57A10.08c |||esterase/lipase |Schizosaccharomyces pombe|chr ...    26   6.3  
SPBC21.05c |ral2||Ras guanyl-nucleotide exchange factor Ral2 |Sc...    25   8.3  

>SPAC22H12.03 |||mitochondrial hydrolase|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 270

 Score = 41.1 bits (92), Expect = 2e-04
 Identities = 37/113 (32%), Positives = 54/113 (47%), Gaps = 3/113 (2%)
 Frame = +2

Query: 194 NPPVLLCHGKLDASSGFRPLV---SLLPDSFYYVAIDLPGNGRSDHLPPGVRYTVIDLVP 364
           +PPVL+ HG L +   +R L    S   D   Y AID   +G S  + P + Y+ + L  
Sbjct: 20  HPPVLIFHGLLGSKRNWRSLAKKFSCKLDRDIY-AIDQRCHGDSPCVAP-LSYSAMAL-D 76

Query: 365 AVIKVREHFKWDKFIYIGHSLGVPIGKFFNIAHPGYVTRMVEVDPVPAHHTWP 523
           A   +++H K DK   IGHS+G        +  P  V ++V VD  P +   P
Sbjct: 77  AFQFMKDH-KLDKASIIGHSMGAKTAMVTALKWPDKVEKLVVVDNSPWYQDLP 128


>SPBP4H10.17c |||carboxyl methyl esterase|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 341

 Score = 33.1 bits (72), Expect = 0.041
 Identities = 32/100 (32%), Positives = 47/100 (47%), Gaps = 9/100 (9%)
 Frame = +2

Query: 158 GNIRG-LTWGNPSNPPVLLCHGKLDASSGFRPLVS-LLPDS---FYYVAIDLPGNGRSDH 322
           G I G LT   P    ++L HG   ++  F P+   LL +S     ++A+DL  +G +  
Sbjct: 56  GTINGYLTLPQPDGCLLVLQHGAGSSAMSFAPVTQELLSNSDNKVGFLALDLRAHGETTL 115

Query: 323 LPPG---VRYTVIDLVPAVIKVREHFKWD-KFIYIGHSLG 430
            P     +     D   AV  V+  F+ D K I +GHSLG
Sbjct: 116 EPESDMSLETLSKDFTHAVSYVQRMFELDEKIILVGHSLG 155


>SPAC6G10.03c |||abhydrolase family protein, unknown biological
           role|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 428

 Score = 27.9 bits (59), Expect = 1.6
 Identities = 11/35 (31%), Positives = 21/35 (60%)
 Frame = +2

Query: 398 DKFIYIGHSLGVPIGKFFNIAHPGYVTRMVEVDPV 502
           +K I +GHS+G  +   + + +P  V +++ V PV
Sbjct: 172 EKMILVGHSMGGYLSAVYAMQYPERVEKLLLVSPV 206


>SPBC1105.06 |pmc4|med4|RNA polymerase II holoenzyme component Pmc4
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 239

 Score = 26.6 bits (56), Expect = 3.6
 Identities = 13/33 (39%), Positives = 17/33 (51%)
 Frame = +2

Query: 449 FNIAHPGYVTRMVEVDPVPAHHTWPTTRQGMRK 547
           F+ A PGY     +    P H+ WP+  Q MRK
Sbjct: 117 FSSAPPGYNPETGQDAKAPVHYPWPSEDQ-MRK 148


>SPBC3B8.06 |||conserved fungal protein|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 511

 Score = 26.2 bits (55), Expect = 4.7
 Identities = 11/44 (25%), Positives = 21/44 (47%)
 Frame = +2

Query: 320 HLPPGVRYTVIDLVPAVIKVREHFKWDKFIYIGHSLGVPIGKFF 451
           H+P  + Y ++ L+  +     H K     Y   ++  P+G+FF
Sbjct: 58  HIPVQIVYVILTLIGYIFAHISHHKASPENYYKGNIHRPVGRFF 101


>SPAC57A10.08c |||esterase/lipase |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 364

 Score = 25.8 bits (54), Expect = 6.3
 Identities = 25/83 (30%), Positives = 34/83 (40%), Gaps = 8/83 (9%)
 Frame = +2

Query: 215 HGKLDASSGFRPLVSLLPDSFYYVAIDLPGNGRSDHLPPGVRYTVID------LVPAVIK 376
           HG     S F+ ++S  P +    + D  G G S    P  R   +D      L     K
Sbjct: 106 HGLGGQMSQFQKVMSYFPPTACLFSFDYWGCGLSRQAFPNQRIGSVDQLTTKGLSKLTYK 165

Query: 377 VREHF--KWDKFIYIGHSLGVPI 439
           V E    +  +FI IGHS+G  I
Sbjct: 166 VLEKLFPENTQFILIGHSMGATI 188


>SPBC21.05c |ral2||Ras guanyl-nucleotide exchange factor Ral2
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 611

 Score = 25.4 bits (53), Expect = 8.3
 Identities = 12/50 (24%), Positives = 27/50 (54%), Gaps = 3/50 (6%)
 Frame = +2

Query: 221 KLDASSGFRPLVSLLPD---SFYYVAIDLPGNGRSDHLPPGVRYTVIDLV 361
           +LD +   +   +L  D   SF ++ +D+     S  +PPG+R+  ++++
Sbjct: 271 ELDTAESEKKSAALAKDNNQSFRFLELDVTEKFLSSAMPPGLRFPAVNIL 320


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,969,472
Number of Sequences: 5004
Number of extensions: 64329
Number of successful extensions: 140
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 137
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 139
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 339215786
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)