Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmmt1i12
(735 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC25H2.03 |||vacuolar protein involved in phosphoinositide met... 128 8e-31
SPCC1840.03 |sal3|pse1|karyopherin Sal3|Schizosaccharomyces pomb... 34 0.024
SPAC27F1.09c |prp10|sap155|U2 snRNP-associated protein Sap155|Sc... 32 0.098
SPAC18G6.05c |||translation elongation regulator Gcn1 |Schizosac... 32 0.098
SPBC15C4.05 |||ATP-dependent RNA/DNA helicase |Schizosaccharomyc... 28 1.2
SPAP8A3.09c |paa1||protein phosphatase regulatory subunit Paa1|S... 28 1.6
SPAC4G8.09 |||mitochondrial leucine-tRNA ligase|Schizosaccharomy... 27 2.8
SPBC839.16 |||C-1-tetrahydrofolate synthase|Schizosaccharomyces ... 27 3.7
SPAC1002.09c |dld1|dldh|dihydrolipoamide dehydrogenase Dld1|Schi... 26 6.4
SPAPB8E5.07c |||ribosome biogenesis protein Rrp12|Schizosaccharo... 26 6.4
SPCC825.01 |||ribosome biogenesis ATPase, Arb family |Schizosacc... 26 6.4
SPBC19C2.01 |cdc28|prp8|ATP-dependent RNA helicase Cdc28 |Schizo... 26 6.4
SPAC56F8.06c |alg10||dolichyl-phosphate-glucose-glycolipid alpha... 26 6.4
SPBC16E9.14c |||membrane transporter|Schizosaccharomyces pombe|c... 25 8.5
SPAC1002.03c |gls2||glucosidase II Gls2|Schizosaccharomyces pomb... 25 8.5
>SPBC25H2.03 |||vacuolar protein involved in phosphoinositide
metabolism|Schizosaccharomyces pombe|chr 2|||Manual
Length = 811
Score = 128 bits (309), Expect = 8e-31
Identities = 63/150 (42%), Positives = 94/150 (62%), Gaps = 2/150 (1%)
Frame = +1
Query: 292 VRGLCDKLYEKRKVAGVEIEKMVKDFNDAKNTSQIKKLIKVLGQDLMSST--NPNMKNGA 465
VRGL KLY+KRK E+E++VK + + T +I+ +I L D + S PN G
Sbjct: 6 VRGLTHKLYDKRKATAYELERVVKGYLENDETEKIRAVISQLANDFVYSPARGPNATFGG 65
Query: 466 LMGLSTVAVGLGKASVDYLPELTNPIVACFSESESRVRYQAAEALFNVLKIVRGASLSQF 645
L+GL+ VA+ LG Y+ + P++ CF++S+S++RY A E+++N+ K+ +G F
Sbjct: 66 LIGLAAVAIALGPKIDSYMESILLPVLYCFNDSDSKIRYYACESMYNIGKVAKGEVFRYF 125
Query: 646 PVIFDALAKLAADPEQQVKHAAELLDRLLK 735
+IFD L KL AD E VK+ AELLDRL+K
Sbjct: 126 NLIFDVLCKLFADTEITVKNGAELLDRLIK 155
>SPCC1840.03 |sal3|pse1|karyopherin Sal3|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 1095
Score = 33.9 bits (74), Expect = 0.024
Identities = 25/97 (25%), Positives = 47/97 (48%), Gaps = 2/97 (2%)
Frame = +1
Query: 427 LMSSTNPNMKNGALMGLSTVAVGLGKASVDYLPELTNPIVACFSESESRVRYQAAEALFN 606
L+ S + ++ ALM +S++A G K L + + ++ ++ RVR+ A A+
Sbjct: 379 LIPSQKWSERHAALMAISSIAEGAEKLMKKELSRVLDMVLPLLADPHPRVRWAACNAVGQ 438
Query: 607 VLKIVRGASLSQFPV-IFDALAKLAADPEQQVK-HAA 711
+ ++P I +AL + PE +V+ HAA
Sbjct: 439 MSTDFAPDMQVKYPSRILEALVPVLESPESRVQAHAA 475
>SPAC27F1.09c |prp10|sap155|U2 snRNP-associated protein
Sap155|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1188
Score = 31.9 bits (69), Expect = 0.098
Identities = 22/112 (19%), Positives = 54/112 (48%), Gaps = 1/112 (0%)
Frame = +1
Query: 292 VRGLCDKLYEKRKVAGVEIEKMVKDFNDAKNTSQIKKLIKVLGQDLMSSTNPNMKNGALM 471
V D+ RK+ ++K++ ++ ++++L+ L L + +++ ++
Sbjct: 738 VNNFKDESEPYRKMTAETVDKVIGSLGVSEIDERLEELL--LDGVLFAFQEQSVEEKVIL 795
Query: 472 G-LSTVAVGLGKASVDYLPELTNPIVACFSESESRVRYQAAEALFNVLKIVR 624
STV LG YLP++ + I+ + + VR QAA+ + ++ +++
Sbjct: 796 TCFSTVVNALGTRCKPYLPQIVSTILYRLNNKSANVREQAADLVSSITIVLK 847
>SPAC18G6.05c |||translation elongation regulator Gcn1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2670
Score = 31.9 bits (69), Expect = 0.098
Identities = 23/78 (29%), Positives = 37/78 (47%), Gaps = 1/78 (1%)
Frame = +1
Query: 301 LCDKLYEKRKVAGVEIEKMVKDFNDAKNTSQIKKLIKVLGQDLMSSTNPNMKNGALMGLS 480
L D + + R A + +++ + K + I +L VL S + + GA GLS
Sbjct: 1650 LIDPVPDTRATAAKALGSLIEKLGEKKFPTLIPELFNVL----RSECSEVDRQGAAQGLS 1705
Query: 481 TVAVGLGKASV-DYLPEL 531
+ GLG A + D LPE+
Sbjct: 1706 EILAGLGLARLEDVLPEI 1723
>SPBC15C4.05 |||ATP-dependent RNA/DNA helicase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1428
Score = 28.3 bits (60), Expect = 1.2
Identities = 16/58 (27%), Positives = 27/58 (46%)
Frame = -2
Query: 503 LPSPTATVDRPISAPFFILGLVEDIKSWPKTLINFFIWLVFFASLKSFTIFSISTPAT 330
L +PT+ + AP +G + P+TL+N + + S + F +F PAT
Sbjct: 577 LETPTSATTSKLMAPMDNIGKFSGFERPPETLLNKWRQQLESESAEKFKVFRNQLPAT 634
>SPAP8A3.09c |paa1||protein phosphatase regulatory subunit
Paa1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 590
Score = 27.9 bits (59), Expect = 1.6
Identities = 20/60 (33%), Positives = 29/60 (48%)
Frame = +1
Query: 529 LTNPIVACFSESESRVRYQAAEALFNVLKIVRGASLSQFPVIFDALAKLAADPEQQVKHA 708
L P+ + S+S R RY A + K+V G SL + +I L D EQ+V+ A
Sbjct: 246 LLQPLRSFVSDSSWRTRYMVAANFVKLAKVV-GPSLIKDELI-KPFVLLMKDTEQEVRRA 303
>SPAC4G8.09 |||mitochondrial leucine-tRNA ligase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 874
Score = 27.1 bits (57), Expect = 2.8
Identities = 29/102 (28%), Positives = 43/102 (42%), Gaps = 12/102 (11%)
Frame = +1
Query: 289 CVRGLCDKLYEKRKVAGVEIEKM--VKDFNDAKNTSQIKKLIK--------VLGQDLMSS 438
CV L ++ EK+ + K+ V D N K SQ + I V +L+ S
Sbjct: 685 CVHQLLER--EKKMSDAMRQTKLTIVDDHNSRKLESQYNEFISQCSAHYENVFSLNLVIS 742
Query: 439 TNPNMKNGALMGLSTVAVGLG--KASVDYLPELTNPIVACFS 558
+ N L V +G KAS++ L + PI+ CFS
Sbjct: 743 DAMKLTNNIADALKNNKVNIGTIKASLEVLVKCIAPIIPCFS 784
>SPBC839.16 |||C-1-tetrahydrofolate synthase|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 937
Score = 26.6 bits (56), Expect = 3.7
Identities = 20/69 (28%), Positives = 28/69 (40%), Gaps = 3/69 (4%)
Frame = +1
Query: 532 TNPIVACFSESESRVRYQAAEALFNVLKIVRGASLSQFPVIFDALAKLAAD---PEQQVK 702
TN + A Q+ AL+ L +V+G PV+F L KL D PE+ +
Sbjct: 446 TNLLAAAIDTRMFHENTQSDAALYKRLTLVKGNKREFAPVMFRRLKKLGIDKTNPEELTE 505
Query: 703 HAAELLDRL 729
RL
Sbjct: 506 EEQRKFARL 514
>SPAC1002.09c |dld1|dldh|dihydrolipoamide dehydrogenase
Dld1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 511
Score = 25.8 bits (54), Expect = 6.4
Identities = 24/87 (27%), Positives = 40/87 (45%), Gaps = 1/87 (1%)
Frame = +1
Query: 319 EKRKVAGVEI-EKMVKDFNDAKNTSQIKKLIKVLGQDLMSSTNPNMKNGALMGLSTVAVG 495
E + GV I EK + A + S++ K + VLG ++ G MG +G
Sbjct: 190 EVKPFPGVTIDEKKIVSSTGALSLSEVPKKMTVLGGGII---------GLEMGSVWSRLG 240
Query: 496 LGKASVDYLPELTNPIVACFSESESRV 576
V++LP + P+ A S++ SR+
Sbjct: 241 AEVTVVEFLPAVGGPMDADISKALSRI 267
>SPAPB8E5.07c |||ribosome biogenesis protein
Rrp12|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1163
Score = 25.8 bits (54), Expect = 6.4
Identities = 17/84 (20%), Positives = 34/84 (40%)
Frame = +1
Query: 364 DFNDAKNTSQIKKLIKVLGQDLMSSTNPNMKNGALMGLSTVAVGLGKASVDYLPELTNPI 543
D+ A T +K++ + L + + + L L+ + V +P+L
Sbjct: 724 DYGKAYATQHVKEIFEELSS-VADRVVSSTRKDRLASLNALYELQSSELVIAIPQLLPEA 782
Query: 544 VACFSESESRVRYQAAEALFNVLK 615
+ E + R+ A + LFN+ K
Sbjct: 783 IISLKEVNEKARHTAFQLLFNIAK 806
>SPCC825.01 |||ribosome biogenesis ATPase, Arb family
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 822
Score = 25.8 bits (54), Expect = 6.4
Identities = 12/54 (22%), Positives = 29/54 (53%)
Frame = +1
Query: 238 TIPYTMSERDYAPLSIACVRGLCDKLYEKRKVAGVEIEKMVKDFNDAKNTSQIK 399
++ + + +R+Y P + CV + D ++RK +E M++D D + + ++
Sbjct: 331 SLDFYLLDREYIPNELTCVEAVLDINEQERK----HLEAMMEDLLDDPDKNAVE 380
>SPBC19C2.01 |cdc28|prp8|ATP-dependent RNA helicase Cdc28
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1055
Score = 25.8 bits (54), Expect = 6.4
Identities = 23/53 (43%), Positives = 28/53 (52%)
Frame = +1
Query: 559 ESESRVRYQAAEALFNVLKIVRGASLSQFPVIFDALAKLAADPEQQVKHAAEL 717
E E R+R + EA N + V S S+F AL KLA DPE K A+EL
Sbjct: 186 EFEERLRRKDLEAATN--EFVEDYS-SKFSSEELALRKLADDPESWRKLASEL 235
>SPAC56F8.06c |alg10||dolichyl-phosphate-glucose-glycolipid
alpha-glucosyltransferase Alg10|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 445
Score = 25.8 bits (54), Expect = 6.4
Identities = 9/18 (50%), Positives = 12/18 (66%)
Frame = -2
Query: 407 INFFIWLVFFASLKSFTI 354
IN+F+W FF S S+ I
Sbjct: 259 INYFLWFFFFFSFPSYII 276
>SPBC16E9.14c |||membrane transporter|Schizosaccharomyces pombe|chr
2|||Manual
Length = 386
Score = 25.4 bits (53), Expect = 8.5
Identities = 12/38 (31%), Positives = 20/38 (52%)
Frame = -2
Query: 503 LPSPTATVDRPISAPFFILGLVEDIKSWPKTLINFFIW 390
L S AT+ +S + G++ I W +TL +F +W
Sbjct: 112 LLSGDATILLTLSCSLIVEGVLIIINVWRETLDSFLVW 149
>SPAC1002.03c |gls2||glucosidase II Gls2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 923
Score = 25.4 bits (53), Expect = 8.5
Identities = 16/52 (30%), Positives = 24/52 (46%)
Frame = -2
Query: 599 NASAAWYLTRDSLSLKQATIGFVNSGK*STDALPSPTATVDRPISAPFFILG 444
+ S WY +L L F+ G ++D S +A V RP+ P F +G
Sbjct: 332 STSTHWYSESGTLDL------FIFLGPKASDVYESYSALVGRPLLPPLFSIG 377
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,794,067
Number of Sequences: 5004
Number of extensions: 53567
Number of successful extensions: 143
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 135
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 142
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 347244562
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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