Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmmt19b04
(755 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef... 367 e-103
SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef... 367 e-103
SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef... 367 e-103
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 110 2e-25
SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related prote... 81 2e-16
SPBC9B6.04c |tuf1||mitochondrial translation elongation factor E... 73 6e-14
SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces po... 28 1.3
SPBC21D10.06c |map4||cell agglutination protein Map4|Schizosacch... 27 2.9
SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subuni... 27 3.8
SPAC19G12.16c |adg2|SPAC23A1.01c, mug46|conserved fungal protein... 26 6.7
SPAC6F12.17 |rna14||mRNA cleavage and polyadenylation specificit... 25 8.8
SPAC631.01c |acp2||F-actin capping protein beta subunit |Schizos... 25 8.8
>SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha
Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual
Length = 460
Score = 367 bits (904), Expect = e-103
Identities = 168/212 (79%), Positives = 189/212 (89%)
Frame = +1
Query: 1 EALDAILPPARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGTIVVFAPANITTEV 180
EA+D+I PPARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGV+KPG IV FAPA +TTEV
Sbjct: 228 EAIDSIEPPARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVIKPGMIVTFAPAGVTTEV 287
Query: 181 KSVEMHHEALQEAVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPKGAADFTAQVIVLNH 360
KSVEMHHE+L +PGDNVGFNVKNVSVK++RRG V GDSKN+PP G A FTAQVI+LNH
Sbjct: 288 KSVEMHHESLDAGLPGDNVGFNVKNVSVKDIRRGNVCGDSKNDPPMGCASFTAQVIILNH 347
Query: 361 PGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKP 540
PGQIS GY+PVLDCHTAHIACKFAE+ EK+DRR+GK E +PK +KSGDA I +VPSKP
Sbjct: 348 PGQISAGYSPVLDCHTAHIACKFAELIEKIDRRSGKKIEESPKFVKSGDACIAKMVPSKP 407
Query: 541 LCVESFQEFPPLGRFAVRDMRQTVAVGVIKAV 636
+CVE+F ++ PLGRFAVRDMRQTVAVGVIKAV
Sbjct: 408 MCVEAFTDYAPLGRFAVRDMRQTVAVGVIKAV 439
>SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha
Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual
Length = 460
Score = 367 bits (904), Expect = e-103
Identities = 168/212 (79%), Positives = 189/212 (89%)
Frame = +1
Query: 1 EALDAILPPARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGTIVVFAPANITTEV 180
EA+D+I PPARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGV+KPG IV FAPA +TTEV
Sbjct: 228 EAIDSIEPPARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVIKPGMIVTFAPAGVTTEV 287
Query: 181 KSVEMHHEALQEAVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPKGAADFTAQVIVLNH 360
KSVEMHHE+L +PGDNVGFNVKNVSVK++RRG V GDSKN+PP G A FTAQVI+LNH
Sbjct: 288 KSVEMHHESLDAGLPGDNVGFNVKNVSVKDIRRGNVCGDSKNDPPMGCASFTAQVIILNH 347
Query: 361 PGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKP 540
PGQIS GY+PVLDCHTAHIACKFAE+ EK+DRR+GK E +PK +KSGDA I +VPSKP
Sbjct: 348 PGQISAGYSPVLDCHTAHIACKFAELIEKIDRRSGKKIEESPKFVKSGDACIAKMVPSKP 407
Query: 541 LCVESFQEFPPLGRFAVRDMRQTVAVGVIKAV 636
+CVE+F ++ PLGRFAVRDMRQTVAVGVIKAV
Sbjct: 408 MCVEAFTDYAPLGRFAVRDMRQTVAVGVIKAV 439
>SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha
Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual
Length = 460
Score = 367 bits (904), Expect = e-103
Identities = 168/212 (79%), Positives = 189/212 (89%)
Frame = +1
Query: 1 EALDAILPPARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGTIVVFAPANITTEV 180
EA+D+I PPARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGV+KPG IV FAPA +TTEV
Sbjct: 228 EAIDSIEPPARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVIKPGMIVTFAPAGVTTEV 287
Query: 181 KSVEMHHEALQEAVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPKGAADFTAQVIVLNH 360
KSVEMHHE+L +PGDNVGFNVKNVSVK++RRG V GDSKN+PP G A FTAQVI+LNH
Sbjct: 288 KSVEMHHESLDAGLPGDNVGFNVKNVSVKDIRRGNVCGDSKNDPPMGCASFTAQVIILNH 347
Query: 361 PGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKP 540
PGQIS GY+PVLDCHTAHIACKFAE+ EK+DRR+GK E +PK +KSGDA I +VPSKP
Sbjct: 348 PGQISAGYSPVLDCHTAHIACKFAELIEKIDRRSGKKIEESPKFVKSGDACIAKMVPSKP 407
Query: 541 LCVESFQEFPPLGRFAVRDMRQTVAVGVIKAV 636
+CVE+F ++ PLGRFAVRDMRQTVAVGVIKAV
Sbjct: 408 MCVEAFTDYAPLGRFAVRDMRQTVAVGVIKAV 439
>SPCC584.04 |sup35|erf3|translation release factor eRF3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 662
Score = 110 bits (265), Expect = 2e-25
Identities = 67/215 (31%), Positives = 113/215 (52%), Gaps = 2/215 (0%)
Frame = +1
Query: 1 EALDAILPPARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGTIVVFAPANITTEV 180
E LD++ R + P +P+ YK +GT+ G++E G +K + V+ P N T EV
Sbjct: 453 EYLDSMTHLERKVNAPFIMPIASKYK--DLGTILEGKIEAGSIKKNSNVLVMPINQTLEV 510
Query: 181 KSV-EMHHEALQEAVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPKGAADFTAQVIVLN 357
++ + E + ++ GD V V+ +++ GYV +KN P F AQ+ +L
Sbjct: 511 TAIYDEADEEISSSICGDQVRLRVRGDD-SDVQTGYVLTSTKN-PVHATTRFIAQIAILE 568
Query: 358 HPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSK 537
P ++ GY+ V+ HTA FA++ K+D+ T + ++ P G I L
Sbjct: 569 LPSILTTGYSCVMHIHTAVEEVSFAKLLHKLDK-TNRKSKKPPMFATKGMKIIAELETQT 627
Query: 538 PLCVESFQEFPPLGRFAVRDMRQTVAVG-VIKAVN 639
P+C+E F+++ +GRF +RD TVAVG V+K ++
Sbjct: 628 PVCMERFEDYQYMGRFTLRDQGTTVAVGKVVKILD 662
>SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 592
Score = 81.0 bits (191), Expect = 2e-16
Identities = 65/210 (30%), Positives = 100/210 (47%), Gaps = 1/210 (0%)
Frame = +1
Query: 4 ALDAILPPARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGTIVVFAPANITTEVK 183
ALD ++PP +P KPLRL + DVY+ TV GRVE G ++ ++ + VK
Sbjct: 390 ALDQLVPPEKPYRKPLRLSIDDVYRSPRSVTV-TGRVEAGNVQVNQVLYDVSSQEDAYVK 448
Query: 184 SVEMHHEALQE-AVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPKGAADFTAQVIVLNH 360
+V + + AV GD V + ++ V +LR G + + + NP + F A++ +
Sbjct: 449 NVIRNSDPSSTWAVAGDTVTLQLADIEVNQLRPGDILSNYE-NPVRRVRSFVAEIQTFDI 507
Query: 361 PGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKP 540
G I +G T VL H+ + K+ K + + S K I L P
Sbjct: 508 HGPILSGSTLVL-----HLGRTVTSVSLKIVTVNNKRSR-HIASRKRALVRISFLDGLFP 561
Query: 541 LCVESFQEFPPLGRFAVRDMRQTVAVGVIK 630
LC+ +E P LGRF +R TVA G++K
Sbjct: 562 LCLA--EECPALGRFILRRSGDTVAAGIVK 589
>SPBC9B6.04c |tuf1||mitochondrial translation elongation factor
EF-Tu Tuf1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 439
Score = 72.5 bits (170), Expect = 6e-14
Identities = 57/212 (26%), Positives = 97/212 (45%), Gaps = 3/212 (1%)
Frame = +1
Query: 1 EALDAILP-PARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGT--IVVFAPANIT 171
EA+D+ + P R TD P + ++DV+ I G GTV GRVE G LK G +V +++
Sbjct: 236 EAVDSYITLPERKTDVPFLMAIEDVFSISGRGTVVTGRVERGTLKKGAEIEIVGYGSHLK 295
Query: 172 TEVKSVEMHHEALQEAVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPKGAADFTAQVIV 351
T V +EM + L AV GDN G ++++ ++L+RG + P F A +
Sbjct: 296 TTVTGIEMFKKQLDAAVAGDNCGLLLRSIKREQLKRGMIVAQPGTVAPH--QKFKASFYI 353
Query: 352 LNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVP 531
L + T +D + + + +++ ++ T + K + GD +
Sbjct: 354 LTK--EEGGRRTGFVDKYRPQLYSRTSDVTVEL---THPDPNDSDKMVMPGDNVEMICTL 408
Query: 532 SKPLCVESFQEFPPLGRFAVRDMRQTVAVGVI 627
P+ +E Q RF VR+ TV ++
Sbjct: 409 IHPIVIEKGQ------RFTVREGGSTVGTALV 434
>SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 646
Score = 28.3 bits (60), Expect = 1.3
Identities = 24/91 (26%), Positives = 40/91 (43%), Gaps = 5/91 (5%)
Frame = +1
Query: 1 EALDAILPPARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGTIVVFAPANITTEV 180
E + I PP + PLR L D + G + + R+ G +K G V+ EV
Sbjct: 228 EIIQKIPPPKGSENAPLRCLLIDSWYNSYQGVIGLVRIMEGFIKKGGKVMSVNTGRKYEV 287
Query: 181 KSVEMHHEALQEA--VPGDNVGF---NVKNV 258
+ V + + + E + VG+ N+KN+
Sbjct: 288 QQVGIMYPDMTEVSRLRAGQVGYIIWNMKNI 318
>SPBC21D10.06c |map4||cell agglutination protein
Map4|Schizosaccharomyces pombe|chr 2|||Manual
Length = 948
Score = 27.1 bits (57), Expect = 2.9
Identities = 14/39 (35%), Positives = 22/39 (56%)
Frame = -1
Query: 179 TSVVMLAGAKTTMVPGFNTPVSTLPTGTVPIPPILYTSC 63
+SVV+ + +T V + + VST TGTV +P +C
Sbjct: 86 SSVVLYSAKETVTVSSYWSLVSTSVTGTVYVPYTSSVAC 124
>SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subunit a
Pol2 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 2199
Score = 26.6 bits (56), Expect = 3.8
Identities = 12/25 (48%), Positives = 15/25 (60%)
Frame = -3
Query: 273 QFLDGHVLYVETYIVSRYSFLESFV 199
+F DGH+L ETY+ LES V
Sbjct: 527 KFFDGHLLASETYVGGHVESLESGV 551
>SPAC19G12.16c |adg2|SPAC23A1.01c, mug46|conserved fungal
protein|Schizosaccharomyces pombe|chr 1|||Manual
Length = 670
Score = 25.8 bits (54), Expect = 6.7
Identities = 24/85 (28%), Positives = 42/85 (49%), Gaps = 2/85 (2%)
Frame = -1
Query: 680 SAALVTLPPPASLKLTALMTPTATVCLMSRTAKRPRGGNSWKDSTH--RGLEGTKLTMAA 507
S +VTLPPPAS ++ T T T + S ++ G+ + +++ + ++++
Sbjct: 183 STDIVTLPPPAS-STSSFSTITNTSMIPSSSSFTTTTGSPYYNTSSFLPSSVISSASLSS 241
Query: 506 SPDLMDFGLTSVDLPVRRSTFSLIS 432
S L +TS PV S+ SL S
Sbjct: 242 SSVLPTSIITSTSTPVTVSSSSLSS 266
>SPAC6F12.17 |rna14||mRNA cleavage and polyadenylation specificity
factor complex subunit Rna14|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 733
Score = 25.4 bits (53), Expect = 8.8
Identities = 10/18 (55%), Positives = 12/18 (66%)
Frame = -1
Query: 128 NTPVSTLPTGTVPIPPIL 75
N P S LPT VP+P I+
Sbjct: 666 NPPTSALPTVPVPLPSII 683
>SPAC631.01c |acp2||F-actin capping protein beta subunit
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 268
Score = 25.4 bits (53), Expect = 8.8
Identities = 11/25 (44%), Positives = 18/25 (72%)
Frame = -1
Query: 515 MAASPDLMDFGLTSVDLPVRRSTFS 441
++ +PDL D L+SVD P++ +T S
Sbjct: 27 LSVAPDLADVLLSSVDQPLKVNTCS 51
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,181,832
Number of Sequences: 5004
Number of extensions: 67409
Number of successful extensions: 211
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 195
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 207
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 361294920
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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