Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmmt18j04
(689 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC7D4.07c |trx1||cytosolic thioredoxin Trx1 |Schizosaccharomyc... 72 7e-14
SPBC12D12.07c |trx2||mitochondrial thioredoxin Trx2|Schizosaccha... 57 3e-09
SPBC577.08c |txl1|trx3|thioredoxin-like I protein Txl1|Schizosac... 56 5e-09
SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces po... 52 8e-08
SPBC26H8.06 |grx4||glutaredoxin Grx4|Schizosaccharomyces pombe|c... 47 2e-06
SPAC17H9.14c |||protein disulfide isomerase|Schizosaccharomyces ... 44 2e-05
SPAC13F5.05 |||thioredoxin family protein|Schizosaccharomyces po... 32 0.068
SPBC3D6.13c |||protein disulfide isomerase |Schizosaccharomyces ... 30 0.36
SPAC167.07c ||SPAC57A7.03c|ubiquitin-protein ligase E3 |Schizosa... 25 7.8
SPBC947.10 |||ubiquitin-protein ligase E3 |Schizosaccharomyces p... 25 7.8
SPAC14C4.15c ||SPAPJ760.01c|dipeptidyl aminopeptidase |Schizosac... 25 7.8
>SPAC7D4.07c |trx1||cytosolic thioredoxin Trx1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 103
Score = 72.1 bits (169), Expect = 7e-14
Identities = 34/90 (37%), Positives = 51/90 (56%)
Frame = +1
Query: 118 IKDSDDLKTRLAEAGDKLVVIDFMATWCGPCKMIGPKLDEIAAEMXXXXXXXXXXXXXXX 297
+ DS + K+ + + DKLVV+DF ATWCGPCK I PK ++ +
Sbjct: 5 VSDSSEFKSIVCQ--DKLVVVDFFATWCGPCKAIAPKFEQF-SNTYSDATFIKVDVDQLS 61
Query: 298 XXASEYNINSMPTFVFVKNGKKLDEFSGAN 387
A+E +++MP+F KNG+K++E GAN
Sbjct: 62 EIAAEAGVHAMPSFFLYKNGEKIEEIVGAN 91
>SPBC12D12.07c |trx2||mitochondrial thioredoxin
Trx2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 121
Score = 56.8 bits (131), Expect = 3e-09
Identities = 28/91 (30%), Positives = 47/91 (51%)
Frame = +1
Query: 118 IKDSDDLKTRLAEAGDKLVVIDFMATWCGPCKMIGPKLDEIAAEMXXXXXXXXXXXXXXX 297
++ D TR++ DK+ V+DF A WCGPCK + P L+++ +E
Sbjct: 22 VESFGDYNTRIS--ADKVTVVDFYADWCGPCKYLKPFLEKL-SEQNQKASFIAVNADKFS 78
Query: 298 XXASEYNINSMPTFVFVKNGKKLDEFSGANV 390
A + + ++PT V + G++LD GA+V
Sbjct: 79 DIAQKNGVYALPTMVLFRKGQELDRIVGADV 109
>SPBC577.08c |txl1|trx3|thioredoxin-like I protein
Txl1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 290
Score = 56.0 bits (129), Expect = 5e-09
Identities = 26/73 (35%), Positives = 38/73 (52%), Gaps = 1/73 (1%)
Frame = +1
Query: 172 VVIDFMATWCGPCKMIGPKLDEIAAEMXXXXXXXXXXXXXXXXX-ASEYNINSMPTFVFV 348
+ +D A WCGPCK I P ++A++ AS + +MPTFVF
Sbjct: 22 LAVDCYADWCGPCKAISPLFSQLASKYASPKFVFAKVNVDEQRQIASGLGVKAMPTFVFF 81
Query: 349 KNGKKLDEFSGAN 387
+NGK++D +GAN
Sbjct: 82 ENGKQIDMLTGAN 94
>SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 492
Score = 52.0 bits (119), Expect = 8e-08
Identities = 24/74 (32%), Positives = 37/74 (50%), Gaps = 1/74 (1%)
Frame = +1
Query: 163 DKLVVIDFMATWCGPCKMIGPKLDEIAAEM-XXXXXXXXXXXXXXXXXASEYNINSMPTF 339
DK++++ F A WCG CK + P+ + A E+ SEY+I PT
Sbjct: 39 DKVLMVKFYAPWCGHCKALAPEYESAADELEKDGISLVEVDCTEEGDLCSEYSIRGYPTL 98
Query: 340 VFVKNGKKLDEFSG 381
KNGK++ ++SG
Sbjct: 99 NVFKNGKQISQYSG 112
Score = 36.7 bits (81), Expect = 0.003
Identities = 19/68 (27%), Positives = 31/68 (45%)
Frame = +1
Query: 166 KLVVIDFMATWCGPCKMIGPKLDEIAAEMXXXXXXXXXXXXXXXXXASEYNINSMPTFVF 345
K V+++F A WCG CK + P +++A E S +I+ PT +F
Sbjct: 374 KDVLVEFYAPWCGHCKNLAPTYEKLAEEYSDDSNVVVAKIDATENDIS-VSISGFPTIMF 432
Query: 346 VKNGKKLD 369
K K++
Sbjct: 433 FKANDKVN 440
>SPBC26H8.06 |grx4||glutaredoxin Grx4|Schizosaccharomyces pombe|chr
2|||Manual
Length = 244
Score = 47.2 bits (107), Expect = 2e-06
Identities = 23/94 (24%), Positives = 42/94 (44%)
Frame = +1
Query: 106 MSIHIKDSDDLKTRLAEAGDKLVVIDFMATWCGPCKMIGPKLDEIAAEMXXXXXXXXXXX 285
MS+ I + + L +++++++F A W PCK + D+ A +
Sbjct: 1 MSVEITFVEQFQEILQNGKEQIILLNFYAPWAAPCKQMNQVFDQFAKD-TKNAVFLKIEA 59
Query: 286 XXXXXXASEYNINSMPTFVFVKNGKKLDEFSGAN 387
A +++N++P FV + K L SGAN
Sbjct: 60 EKFSDIAESFDVNAVPLFVLIHGAKVLARISGAN 93
>SPAC17H9.14c |||protein disulfide isomerase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 359
Score = 44.0 bits (99), Expect = 2e-05
Identities = 28/108 (25%), Positives = 49/108 (45%), Gaps = 3/108 (2%)
Frame = +1
Query: 70 VILFLVSIYLPKMSIHIKDSDDLKTRLAEAGDKLVVIDFMATWCGPCKMIGPKLDEIAA- 246
VI L ++ + ++ ++L+ + A K +I+F ATWCG CK + P +E+ A
Sbjct: 9 VIFALFALVFASGVVELQSLNELENTI-RASKKGALIEFYATWCGHCKSLAPVYEELGAL 67
Query: 247 -EMXXXXXXXXXXXXXXXXXASEYNINSMPTFV-FVKNGKKLDEFSGA 384
E A +Y+I PT + F +G + ++S A
Sbjct: 68 FEDHNDVLIGKIDADTHSDVADKYHITGFPTLIWFPPDGSEPVQYSNA 115
Score = 30.3 bits (65), Expect = 0.27
Identities = 10/25 (40%), Positives = 16/25 (64%)
Frame = +1
Query: 166 KLVVIDFMATWCGPCKMIGPKLDEI 240
K V+++F A WCG CK + P + +
Sbjct: 159 KDVLVEFYADWCGYCKRLAPTYETL 183
>SPAC13F5.05 |||thioredoxin family protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 363
Score = 32.3 bits (70), Expect = 0.068
Identities = 31/121 (25%), Positives = 46/121 (38%), Gaps = 2/121 (1%)
Frame = +1
Query: 31 TLSLACFLFAF*TVILFLVSIYLPKMSIHIKDSDDLKTRLAEAGDKLVVIDFMATWCGPC 210
TL LACF +F S +I + +S + + + G LVV F A WCG C
Sbjct: 10 TLFLACFSLVSG---VFGYSPMFGSNTIEL-NSKNFRKFVKAKGPSLVV--FYAPWCGYC 63
Query: 211 KMIGPKLDEIAAEMXXXXXXXXXXXXXXXXXA--SEYNINSMPTFVFVKNGKKLDEFSGA 384
K + P ++A+ + A S+Y + PT V K S
Sbjct: 64 KKLVPTYQKLASNLHSLLPVTAVDCDADQNRAVCSQYQVQGFPTIKLVYPSSKGSSLSST 123
Query: 385 N 387
+
Sbjct: 124 D 124
>SPBC3D6.13c |||protein disulfide isomerase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 726
Score = 29.9 bits (64), Expect = 0.36
Identities = 24/104 (23%), Positives = 44/104 (42%), Gaps = 3/104 (2%)
Frame = +1
Query: 70 VILFLVSIYLPKMSIHIKDSDDLKTRLAEAGDKLVVIDFMATWCGPCKMIGPKLD---EI 240
V+ F+ + + + D+D L++ +++ I + CG CK +GP D E
Sbjct: 15 VVAFITMVQTLVSGVPLTDND-LESEVSKG---TWFIKYYLPSCGACKRLGPMWDNMVEK 70
Query: 241 AAEMXXXXXXXXXXXXXXXXXASEYNINSMPTFVFVKNGKKLDE 372
A E +S NI ++PT +NG+ ++E
Sbjct: 71 AKEQVEGSNFHFGEVDCSKELSSCANIRAVPTLYLYQNGEIVEE 114
>SPAC167.07c ||SPAC57A7.03c|ubiquitin-protein ligase E3
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1029
Score = 25.4 bits (53), Expect = 7.8
Identities = 9/22 (40%), Positives = 16/22 (72%)
Frame = -1
Query: 557 KLISSNSLQTLKTFFIHLLKTY 492
KL+SSN+LQ + F+ ++K +
Sbjct: 412 KLVSSNTLQAMSHFYATMIKLF 433
>SPBC947.10 |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 676
Score = 25.4 bits (53), Expect = 7.8
Identities = 16/59 (27%), Positives = 30/59 (50%)
Frame = -3
Query: 417 FKDSCFEFVDVSAREFVQFLAILNEDERRHRVNVVLAGDVLALINVHLHNDDGI*HFGG 241
F + +E+VD ++ + + F+ L + ++ GD + INV L + D +FGG
Sbjct: 114 FSANLYEYVDGNS-DGISFVLNLENNNDTSVYHMTFHGDRVKPINVFLGSTDVTPNFGG 171
>SPAC14C4.15c ||SPAPJ760.01c|dipeptidyl aminopeptidase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 853
Score = 25.4 bits (53), Expect = 7.8
Identities = 10/24 (41%), Positives = 16/24 (66%), Gaps = 1/24 (4%)
Frame = +3
Query: 27 LYVVTCVFLIC-ILNCDIIFSFNL 95
+Y+ C+F + +L+C IIF F L
Sbjct: 67 IYLAVCLFFLASVLSCAIIFRFYL 90
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,555,892
Number of Sequences: 5004
Number of extensions: 47702
Number of successful extensions: 153
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 141
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 148
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 319939482
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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