BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmmt18g02
(678 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC1709.05 |sks2|hsc1|heat shock protein Sks2|Schizosaccharomyc... 31 0.12
SPAC17G6.05c |||Rhophilin-2 homolog|Schizosaccharomyces pombe|ch... 31 0.15
SPBC23E6.02 |||ATP-dependent DNA helicase |Schizosaccharomyces p... 29 0.47
SPCC1450.02 ||SPCC191.13|bromodomain protein|Schizosaccharomyces... 29 0.62
SPAC11H11.04 |mam2||pheromone p-factor receptor|Schizosaccharomy... 29 0.82
SPAC1486.04c |alm1||medial ring protein Alm1|Schizosaccharomyces... 28 1.1
SPBC146.05c |cwf25||complexed with Cdc5 protein Cwf25 |Schizosac... 27 2.5
SPBC16A3.19 |||histone acetyltransferase complex subunit Eaf7 |S... 27 2.5
SPAC16E8.13 |||ubiquitin-protein ligase E3 |Schizosaccharomyces ... 27 2.5
SPBC4F6.10 |vps901|vps9a|guanyl-nucleotide exchange factor Vps90... 26 4.4
SPAC1002.03c |gls2||glucosidase II Gls2|Schizosaccharomyces pomb... 26 5.8
SPAC1F3.06c |spo15||sporulation protein Spo15|Schizosaccharomyce... 26 5.8
SPAC1952.03 |||cysteine protease, OTU family|Schizosaccharomyces... 25 7.6
SPCC162.08c |nup211||nuclear pore complex associated protein|Sch... 25 7.6
SPBC23E6.03c |nta1||protein N-terminal amidase Nta1 |Schizosacch... 25 7.6
>SPBC1709.05 |sks2|hsc1|heat shock protein Sks2|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 613
Score = 31.5 bits (68), Expect = 0.12
Identities = 24/98 (24%), Positives = 51/98 (52%), Gaps = 1/98 (1%)
Frame = +3
Query: 153 QSDYPSVMGLLAHLNSNELKEMLNDDAKFESVLKDVKQVKDWDTEKEMFIASNRSLAEFN 332
+S + + + HL+S +++EM+ + KF+ KD + + ++ E +I++ +
Sbjct: 500 RSAHIEITNSVGHLSSTKIQEMIENADKFKQQDKDFAKKLEAKSQLESYISNIETTI--- 556
Query: 333 LGKEPELERMKAELQEKSELGEQLCTRIQEL-LDDYKT 443
EP + MK + +KS++ QL + +L L+D T
Sbjct: 557 --SEPNV-MMKLKRGDKSKIEAQLAECMSQLELEDTNT 591
>SPAC17G6.05c |||Rhophilin-2 homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 775
Score = 31.1 bits (67), Expect = 0.15
Identities = 27/115 (23%), Positives = 53/115 (46%)
Frame = +3
Query: 306 SNRSLAEFNLGKEPELERMKAELQEKSELGEQLCTRIQELLDDYKTKSAGISPDTTHALL 485
S +SL + + +E + MK +L +L E+L +R+Q+L+ + +T + L
Sbjct: 565 SGQSLLDIPIEQEEQERDMKIQL----DLLEELQSRVQKLVPERQTTLQALQ----QKCL 616
Query: 486 QTAAAESEEQSDNIARDFLSGKMGVDKFLEDFEPIRKXMHIRKYKAEKMSELLRN 650
Q +ES Q+ L + L+ F+P+R +H + + + +RN
Sbjct: 617 QDDISESLMQNSKRKDSALDTNQLFELELKKFDPLRNRLHASYRQQQLLLNEMRN 671
>SPBC23E6.02 |||ATP-dependent DNA helicase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1040
Score = 29.5 bits (63), Expect = 0.47
Identities = 15/34 (44%), Positives = 21/34 (61%)
Frame = +3
Query: 525 IARDFLSGKMGVDKFLEDFEPIRKXMHIRKYKAE 626
IA +F KM DK +ED PI+K H+ ++AE
Sbjct: 500 IAYEF-KNKMAYDKSIEDNAPIKKFEHLPFFEAE 532
>SPCC1450.02 ||SPCC191.13|bromodomain protein|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 578
Score = 29.1 bits (62), Expect = 0.62
Identities = 15/57 (26%), Positives = 35/57 (61%)
Frame = +3
Query: 354 ERMKAELQEKSELGEQLCTRIQELLDDYKTKSAGISPDTTHALLQTAAAESEEQSDN 524
E+ K + ++E E++ R+Q+ LD + K++ SP++ +A + +ES+ +S++
Sbjct: 520 EKKKGRVLSETEQAEKI-RRLQQQLDRFAGKTSPTSPESNNA-ANVSDSESDNESES 574
>SPAC11H11.04 |mam2||pheromone p-factor receptor|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 348
Score = 28.7 bits (61), Expect = 0.82
Identities = 11/36 (30%), Positives = 21/36 (58%), Gaps = 2/36 (5%)
Frame = -1
Query: 651 CFSTILTFSRLCTSLYAXV--YGWVQNLLEIYQHPF 550
C IL +C++ Y+ + YG++ N++ +Y H F
Sbjct: 91 CLRAILNIVTICSNSYSILVNYGFILNMVHMYVHVF 126
>SPAC1486.04c |alm1||medial ring protein Alm1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1727
Score = 28.3 bits (60), Expect = 1.1
Identities = 29/115 (25%), Positives = 50/115 (43%), Gaps = 6/115 (5%)
Frame = +3
Query: 330 NLGKEPELERMKAE--LQEKSELGEQLCTRIQELLDDYKTKSAGISPDTTHALLQTAAAE 503
NL E ++K E L E++ L E L T +L SAG D ++ + E
Sbjct: 591 NLESELNSSKIKNESLLNERNLLKEMLATSRSSILSH--NSSAGNIDDKMKSI-DESTRE 647
Query: 504 SEEQSDNIARDFLSGKMGVDK----FLEDFEPIRKXMHIRKYKAEKMSELLRNSN 656
E+ + + + + + K L + E IRK + KY+ + ++ L N+N
Sbjct: 648 LEKNYEVYRNEMTAIQESLSKRNQDLLSEMEAIRKELENSKYQQQLSTDRLTNAN 702
>SPBC146.05c |cwf25||complexed with Cdc5 protein Cwf25
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 376
Score = 27.1 bits (57), Expect = 2.5
Identities = 26/110 (23%), Positives = 50/110 (45%)
Frame = +3
Query: 195 NSNELKEMLNDDAKFESVLKDVKQVKDWDTEKEMFIASNRSLAEFNLGKEPELERMKAEL 374
+S+E++E L + + +LKD + ++ EK FIA + + + + L+ + A
Sbjct: 84 DSSEMEEYLLGRRRLDDLLKDKIEDQNNSLEKTEFIALQNANSLQDTQAKLRLDPLLAIK 143
Query: 375 QEKSELGEQLCTRIQELLDDYKTKSAGISPDTTHALLQTAAAESEEQSDN 524
Q++ + + L + + LD + D H Q S E+SDN
Sbjct: 144 QQEQKQLQTLMEKRKYSLDSDRKSKERRHRDRHHRSNQD---RSRERSDN 190
>SPBC16A3.19 |||histone acetyltransferase complex subunit Eaf7
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 272
Score = 27.1 bits (57), Expect = 2.5
Identities = 25/108 (23%), Positives = 52/108 (48%), Gaps = 4/108 (3%)
Frame = +3
Query: 210 KEMLNDDAKFESVLKDVKQVKDWDTEKEMFIASNRSLAEFNLGK-EPELERMKAELQEKS 386
K + + D K + KD+ +VK+ +EK + + + EPE+++ +EK
Sbjct: 110 KRIKSPDVKDFKLPKDILKVKE-KSEKPLETSQKVEIETVETKPGEPEVKQETNLQKEKK 168
Query: 387 ELGEQLCTRIQELLDDYKTKSAGISPDTTH---ALLQTAAAESEEQSD 521
E +L ++ +++ + ++ S ISP T +Q E +E+S+
Sbjct: 169 ESKVKLESKEEKISRNLRSSSRSISPVTEQPQSPKIQPVIPEKKEKSE 216
>SPAC16E8.13 |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 547
Score = 27.1 bits (57), Expect = 2.5
Identities = 13/34 (38%), Positives = 18/34 (52%)
Frame = +3
Query: 348 ELERMKAELQEKSELGEQLCTRIQELLDDYKTKS 449
+LE +K +E+ + E L RIQ L TKS
Sbjct: 435 QLEHLKHNYEEEKSMNENLLVRIQTLEKQNTTKS 468
>SPBC4F6.10 |vps901|vps9a|guanyl-nucleotide exchange factor Vps901
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 537
Score = 26.2 bits (55), Expect = 4.4
Identities = 13/47 (27%), Positives = 25/47 (53%)
Frame = +3
Query: 222 NDDAKFESVLKDVKQVKDWDTEKEMFIASNRSLAEFNLGKEPELERM 362
+DD + + VL + ++ W TE+ + I +S ++F EL R+
Sbjct: 216 SDDVEEDRVLSEKMELFQWITEENLDIKKQKSSSKFFKLAADELRRI 262
>SPAC1002.03c |gls2||glucosidase II Gls2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 923
Score = 25.8 bits (54), Expect = 5.8
Identities = 12/27 (44%), Positives = 16/27 (59%)
Frame = +3
Query: 159 DYPSVMGLLAHLNSNELKEMLNDDAKF 239
DY +V G+ H +S LKE N DA +
Sbjct: 242 DYGNVYGVPEHTSSLSLKETNNSDAGY 268
>SPAC1F3.06c |spo15||sporulation protein Spo15|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1957
Score = 25.8 bits (54), Expect = 5.8
Identities = 35/141 (24%), Positives = 59/141 (41%), Gaps = 10/141 (7%)
Frame = +3
Query: 261 KQVKDWDTEKEMFIASNRSLAE---FNLGKEPELERMKAELQEKSELGEQLCTRIQELLD 431
K+ KD++ KE + SLAE N E ER++ L ++ L L
Sbjct: 219 KKEKDYEKIKEDVSSIKASLAEEQASNKSLRGEQERLEKLLVSSNKTVSTLRQTENSLRA 278
Query: 432 DYKTKSAGISPDTTHALLQTAAAESEEQSDNIARDFLSGKMGVDKFLED-------FEPI 590
+ KT + A+ + + EE N+A ++ + DK +ED F+ +
Sbjct: 279 ECKTLQEKLEKC---AINEEDSKLLEELKHNVA-NYSDAIVHKDKLIEDLSTRISEFDNL 334
Query: 591 RKXMHIRKYKAEKMSELLRNS 653
+ K EK+ +LLRN+
Sbjct: 335 KSERDTLSIKNEKLEKLLRNT 355
>SPAC1952.03 |||cysteine protease, OTU family|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 324
Score = 25.4 bits (53), Expect = 7.6
Identities = 13/40 (32%), Positives = 24/40 (60%)
Frame = +3
Query: 339 KEPELERMKAELQEKSELGEQLCTRIQELLDDYKTKSAGI 458
++ LER KAE+++ SE E ++ +L ++ K K + I
Sbjct: 140 QKERLERRKAEMKKMSEQAELESEKMADLKNEEKKKFSKI 179
>SPCC162.08c |nup211||nuclear pore complex associated
protein|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1837
Score = 25.4 bits (53), Expect = 7.6
Identities = 21/71 (29%), Positives = 46/71 (64%), Gaps = 3/71 (4%)
Frame = +3
Query: 186 AHLN---SNELKEMLNDDAKFESVLKDVKQVKDWDTEKEMFIASNRSLAEFNLGKEPELE 356
AHL +N+ KE+ + +A+ E++ K+++ +KD + + + +S+ AE + KE + E
Sbjct: 1433 AHLKQELTNKNKELTSKNAENEAMQKEIESLKDSNHQLQESASSD---AE-QITKE-QFE 1487
Query: 357 RMKAELQEKSE 389
++K+E +E++E
Sbjct: 1488 QLKSE-KERTE 1497
>SPBC23E6.03c |nta1||protein N-terminal amidase Nta1
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 286
Score = 25.4 bits (53), Expect = 7.6
Identities = 15/49 (30%), Positives = 23/49 (46%), Gaps = 3/49 (6%)
Frame = +3
Query: 216 MLNDDAKFESVLKDVKQVKDWDTEKEMFIASNRS---LAEFNLGKEPEL 353
+++DD ++ L D+K + W T I SN L GKE +L
Sbjct: 202 LVSDDKVIDASLPDIKNLHYWTTRLSPLINSNTDAIVLVANRWGKENDL 250
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,361,589
Number of Sequences: 5004
Number of extensions: 41687
Number of successful extensions: 141
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 139
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 141
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 311890690
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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