BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmmt13m04
(643 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC16G5.12c |top3||DNA topoisomerase III|Schizosaccharomyces po... 30 0.33
SPBC16H5.10c |prp43||ATP-dependent RNA helicase Prp43|Schizosacc... 27 2.3
SPBC409.09c |mis13|cnl1|kinetochore protein Mis13|Schizosaccharo... 27 3.0
SPBC21.05c |ral2||Ras guanyl-nucleotide exchange factor Ral2 |Sc... 26 4.0
SPBC418.01c |his4|SPBC887.20c|imidazoleglycerol-phosphate syntha... 26 4.0
SPAC1F7.07c |fip1||iron permease Fip1|Schizosaccharomyces pombe|... 26 4.0
SPAC56F8.16 |esc1||transcription factor Esc1 |Schizosaccharomyce... 26 5.3
SPBC1773.13 |||aromatic aminotransferase |Schizosaccharomyces po... 25 7.0
>SPBC16G5.12c |top3||DNA topoisomerase III|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 622
Score = 29.9 bits (64), Expect = 0.33
Identities = 10/35 (28%), Positives = 23/35 (65%)
Frame = +3
Query: 276 EYSQNPQFKAHVINLMTSLNLAVNNLNQPEIVAAM 380
EY + +F+ H++++M S + + + +PE++A M
Sbjct: 465 EYRLHEEFQPHILDMMDSSTSSPSYITEPELIALM 499
>SPBC16H5.10c |prp43||ATP-dependent RNA helicase
Prp43|Schizosaccharomyces pombe|chr 2|||Manual
Length = 735
Score = 27.1 bits (57), Expect = 2.3
Identities = 27/97 (27%), Positives = 44/97 (45%), Gaps = 3/97 (3%)
Frame = +3
Query: 18 P*RKRIKTRHKQIMGTWFSYMWWGGDPDVVNPVSGLTRREIHAVQKSWAPVNANSFATGS 197
P +K+++ K + +++ G D + V GLTRR Q + A N+F
Sbjct: 3 PAQKKLRQESKN---PYLAHLNNGDDSEEVVSSKGLTRRATTVAQAAKAEEGPNNF---- 55
Query: 198 ELLRRLFNTYPDTKEYFKMV---RKLPEEEYSQNPQF 299
FN P ++ YFK++ R+LP Y Q +F
Sbjct: 56 ------FNDKPFSQNYFKILETRRELP--VYQQREEF 84
>SPBC409.09c |mis13|cnl1|kinetochore protein
Mis13|Schizosaccharomyces pombe|chr 2|||Manual
Length = 329
Score = 26.6 bits (56), Expect = 3.0
Identities = 19/119 (15%), Positives = 48/119 (40%), Gaps = 2/119 (1%)
Frame = +3
Query: 135 EIHAVQKSWAPVNANSFATGSELL--RRLFNTYPDTKEYFKMVRKLPEEEYSQNPQFKAH 308
++H + +W V ANS ++ +L ++ + ++ K LP ++ + P
Sbjct: 194 QLHNEEAAWRAVAANSVSSDKSILSFKKAVESIDSKQDLDKQDSPLPPDDAPELPNISKL 253
Query: 309 VINLMTSLNLAVNNLNQPEIVAAMMTKLGESHRRRQIKEKNFHELKEVIVKLFIDVLRL 485
T L++ N++ + ++ S+ R ++ H + ++D + L
Sbjct: 254 KPKFHTLLDMLAENIHTLHSLTNAGPEVRSSYGRLAAQDFIAHRKSLLSFSKYVDTMNL 312
>SPBC21.05c |ral2||Ras guanyl-nucleotide exchange factor Ral2
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 611
Score = 26.2 bits (55), Expect = 4.0
Identities = 13/44 (29%), Positives = 25/44 (56%)
Frame = +2
Query: 29 EDQNQTQANHGHLV*LHVVGWGSGRGEPGLRAHEERDTRSSKIM 160
+D N Q+N+ H+V + + G+G R +P + ER + K++
Sbjct: 388 QDYNLRQSNYDHIVFIELEGYGVYR-KPQMGRVTERSEQLGKLL 430
>SPBC418.01c |his4|SPBC887.20c|imidazoleglycerol-phosphate
synthase|Schizosaccharomyces pombe|chr 2|||Manual
Length = 541
Score = 26.2 bits (55), Expect = 4.0
Identities = 14/42 (33%), Positives = 19/42 (45%)
Frame = +3
Query: 177 NSFATGSELLRRLFNTYPDTKEYFKMVRKLPEEEYSQNPQFK 302
NS A S+ + F P K YF +PE+ P+FK
Sbjct: 122 NSCAVRSDTSKEFFGMRPHDKFYFVHSYMIPEKGLILPPEFK 163
>SPAC1F7.07c |fip1||iron permease Fip1|Schizosaccharomyces pombe|chr
1|||Manual
Length = 397
Score = 26.2 bits (55), Expect = 4.0
Identities = 10/30 (33%), Positives = 18/30 (60%)
Frame = -1
Query: 640 IHTNSTLFILNHYIISFVCICYLVSSGLFN 551
I+ + L ++I+ CI YL+S+GL +
Sbjct: 196 IYRGGNVMNLQWFLIASTCILYLISAGLMS 225
>SPAC56F8.16 |esc1||transcription factor Esc1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 413
Score = 25.8 bits (54), Expect = 5.3
Identities = 13/45 (28%), Positives = 24/45 (53%)
Frame = +3
Query: 138 IHAVQKSWAPVNANSFATGSELLRRLFNTYPDTKEYFKMVRKLPE 272
++ +S++ +NAN +E LRR+ + P+ EY + PE
Sbjct: 290 VYRAPESFSSINANVPYGRNEYLRRVTSLVPNQPEYTGPYTRNPE 334
>SPBC1773.13 |||aromatic aminotransferase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 481
Score = 25.4 bits (53), Expect = 7.0
Identities = 10/30 (33%), Positives = 19/30 (63%)
Frame = -1
Query: 643 SIHTNSTLFILNHYIISFVCICYLVSSGLF 554
++ N+ L + N Y+ + VCI ++ +GLF
Sbjct: 360 TVRRNAFLNVANKYLPNSVCIYHVPRAGLF 389
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,680,527
Number of Sequences: 5004
Number of extensions: 54499
Number of successful extensions: 155
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 153
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 155
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 287744314
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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