Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmmt12m08
(620 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef... 335 3e-93
SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef... 335 3e-93
SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef... 335 3e-93
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 128 5e-31
SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related prote... 126 3e-30
SPBC9B6.04c |tuf1||mitochondrial translation elongation factor E... 91 9e-20
SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces po... 33 0.044
SPAC1952.11c |ure2||urease |Schizosaccharomyces pombe|chr 1|||Ma... 28 0.95
SPBC24C6.08c |||vesicle coat protein|Schizosaccharomyces pombe|c... 27 2.2
SPBC21D10.06c |map4||cell agglutination protein Map4|Schizosacch... 27 2.2
SPBC17G9.09 |tif213||translation initiation factor eIF2 gamma su... 27 2.9
SPAC23H4.03c |erv25||COPII-coated vesicle component Erv25 |Schiz... 27 2.9
SPAC222.09 |seb1||RNA-binding protein Seb1 |Schizosaccharomyces ... 26 3.8
SPAC6F12.17 |rna14||mRNA cleavage and polyadenylation specificit... 25 6.7
SPAC19G12.08 |||fatty acid hydroxylase |Schizosaccharomyces pomb... 25 6.7
SPAC10F6.05c |ubc6||ubiquitin conjugating enzyme Ubc6|Schizosacc... 25 8.8
SPAC57A7.11 |mip1||WD repeat protein Mip1|Schizosaccharomyces po... 25 8.8
SPBC11B10.05c |rsp1||random septum position protein Rsp1|Schizos... 25 8.8
SPBC1539.08 |||ADP-ribosylation factor, Arf family|Schizosacchar... 25 8.8
>SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha
Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual
Length = 460
Score = 335 bits (824), Expect = 3e-93
Identities = 155/206 (75%), Positives = 178/206 (86%)
Frame = +3
Query: 3 ITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPY 182
ITGTSQADCAVLI+ GTGEFEAGISK+GQTREHALLA+TLGVKQLIV VNKMD+T +
Sbjct: 103 ITGTSQADCAVLIIGGGTGEFEAGISKDGQTREHALLAYTLGVKQLIVAVNKMDTTG--W 160
Query: 183 SEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLEPSTKMPWFKGWQVERKEGK 362
S+ RFEEI KE S++IKK+G+NP V FVP+SG+ GDNM+EP+T MPW++GWQ E K G
Sbjct: 161 SQARFEEIVKETSNFIKKVGFNPKTVPFVPVSGFQGDNMIEPTTNMPWYQGWQKETKAGV 220
Query: 363 ADGKCLIEALDAILPPARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGTIVVFAP 542
GK L+EA+D+I PPARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGV+KPG IV FAP
Sbjct: 221 VKGKTLLEAIDSIEPPARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVIKPGMIVTFAP 280
Query: 543 ANITTEVKSVEMHHEALQEAVPGDNV 620
A +TTEVKSVEMHHE+L +PGDNV
Sbjct: 281 AGVTTEVKSVEMHHESLDAGLPGDNV 306
>SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha
Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual
Length = 460
Score = 335 bits (823), Expect = 3e-93
Identities = 154/206 (74%), Positives = 178/206 (86%)
Frame = +3
Query: 3 ITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPY 182
ITGTSQADCA+LI+ GTGEFEAGISK+GQTREHALLA+TLGVKQLIV VNKMD+T +
Sbjct: 103 ITGTSQADCAILIIGGGTGEFEAGISKDGQTREHALLAYTLGVKQLIVAVNKMDTTG--W 160
Query: 183 SEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLEPSTKMPWFKGWQVERKEGK 362
S+ RFEEI KE S++IKK+G+NP V FVP+SG+ GDNM+EP+T MPW++GWQ E K G
Sbjct: 161 SQARFEEIVKETSNFIKKVGFNPKTVPFVPVSGFQGDNMIEPTTNMPWYQGWQKETKAGV 220
Query: 363 ADGKCLIEALDAILPPARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGTIVVFAP 542
GK L+EA+D+I PPARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGV+KPG IV FAP
Sbjct: 221 VKGKTLLEAIDSIEPPARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVIKPGMIVTFAP 280
Query: 543 ANITTEVKSVEMHHEALQEAVPGDNV 620
A +TTEVKSVEMHHE+L +PGDNV
Sbjct: 281 AGVTTEVKSVEMHHESLDAGLPGDNV 306
>SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha
Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual
Length = 460
Score = 335 bits (823), Expect = 3e-93
Identities = 154/206 (74%), Positives = 178/206 (86%)
Frame = +3
Query: 3 ITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPY 182
ITGTSQADCA+LI+ GTGEFEAGISK+GQTREHALLA+TLGVKQLIV VNKMD+T +
Sbjct: 103 ITGTSQADCAILIIGGGTGEFEAGISKDGQTREHALLAYTLGVKQLIVAVNKMDTTG--W 160
Query: 183 SEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLEPSTKMPWFKGWQVERKEGK 362
S+ RFEEI KE S++IKK+G+NP V FVP+SG+ GDNM+EP+T MPW++GWQ E K G
Sbjct: 161 SQARFEEIVKETSNFIKKVGFNPKTVPFVPVSGFQGDNMIEPTTNMPWYQGWQKETKAGV 220
Query: 363 ADGKCLIEALDAILPPARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGTIVVFAP 542
GK L+EA+D+I PPARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGV+KPG IV FAP
Sbjct: 221 VKGKTLLEAIDSIEPPARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVIKPGMIVTFAP 280
Query: 543 ANITTEVKSVEMHHEALQEAVPGDNV 620
A +TTEVKSVEMHHE+L +PGDNV
Sbjct: 281 AGVTTEVKSVEMHHESLDAGLPGDNV 306
>SPCC584.04 |sup35|erf3|translation release factor eRF3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 662
Score = 128 bits (310), Expect = 5e-31
Identities = 74/211 (35%), Positives = 120/211 (56%), Gaps = 5/211 (2%)
Frame = +3
Query: 3 ITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPY 182
I G SQAD VL+++A GEFEAG + GQTREHA+LA T G+ L+V +NKMD +
Sbjct: 334 INGASQADIGVLVISARRGEFEAGFERGGQTREHAVLARTQGINHLVVVINKMDEPSVQW 393
Query: 183 SEPRFEEIKKEVSSYIKKI-GYNPAA-VAFVPISGWHGDNMLE--PSTKMPWFKGWQVER 350
SE R++E ++S +++++ GYN V ++P+S + G N+ + S+ PW++
Sbjct: 394 SEERYKECVDKLSMFLRRVAGYNSKTDVKYMPVSAYTGQNVKDRVDSSVCPWYQ------ 447
Query: 351 KEGKADGKCLIEALDAILPPARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGTIV 530
G L+E LD++ R + P +P+ YK +GT+ G++E G +K + V
Sbjct: 448 ------GPSLLEYLDSMTHLERKVNAPFIMPIASKYK--DLGTILEGKIEAGSIKKNSNV 499
Query: 531 VFAPANITTEVKSV-EMHHEALQEAVPGDNV 620
+ P N T EV ++ + E + ++ GD V
Sbjct: 500 LVMPINQTLEVTAIYDEADEEISSSICGDQV 530
>SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 592
Score = 126 bits (303), Expect = 3e-30
Identities = 82/210 (39%), Positives = 114/210 (54%), Gaps = 4/210 (1%)
Frame = +3
Query: 3 ITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPY 182
I G S AD AVL+V + FE G +NGQTREHA L LG+ +++V VNK+D +
Sbjct: 273 IAGASSADFAVLVVDSSQNNFERGFLENGQTREHAYLLRALGISEIVVSVNKLDLMS--W 330
Query: 183 SEPRFEEIKKEVSSY-IKKIGYNPAAVAFVPISGWHGDNML--EPSTKMPWFKGWQVERK 353
SE RF+EIK VS + IK +G+ + V FVPIS G N++ + S W+K
Sbjct: 331 SEDRFQEIKNIVSDFLIKMVGFKTSNVHFVPISAISGTNLIQKDSSDLYKWYK------- 383
Query: 354 EGKADGKCLIEALDAILPPARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGTIVV 533
G L+ ALD ++PP +P KPLRL + DVY+ TV GRVE G ++ ++
Sbjct: 384 -----GPTLLSALDQLVPPEKPYRKPLRLSIDDVYRSPRSVTV-TGRVEAGNVQVNQVLY 437
Query: 534 FAPANITTEVKSVEMHHEALQE-AVPGDNV 620
+ VK+V + + AV GD V
Sbjct: 438 DVSSQEDAYVKNVIRNSDPSSTWAVAGDTV 467
>SPBC9B6.04c |tuf1||mitochondrial translation elongation factor
EF-Tu Tuf1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 439
Score = 91.5 bits (217), Expect = 9e-20
Identities = 72/209 (34%), Positives = 107/209 (51%), Gaps = 4/209 (1%)
Frame = +3
Query: 3 ITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPY 182
ITG + D A+++V+A G+ QTREH LLA +GVKQ++V +NK+D EP
Sbjct: 134 ITGAATMDGAIIVVSATDGQMP-------QTREHLLLARQVGVKQIVVYINKVDMVEPDM 186
Query: 183 SEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNM-LEPSTKMPWFKGWQVERKEG 359
E E+++ +S Y G+ GDN + + + +G + E G
Sbjct: 187 IELVEMEMRELLSEY-----------------GFDGDNTPIVSGSALCALEGREPEI--G 227
Query: 360 KADGKCLIEALDAILP-PARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGT--IV 530
L+EA+D+ + P R TD P + ++DV+ I G GTV GRVE G LK G +
Sbjct: 228 LNSITKLMEAVDSYITLPERKTDVPFLMAIEDVFSISGRGTVVTGRVERGTLKKGAEIEI 287
Query: 531 VFAPANITTEVKSVEMHHEALQEAVPGDN 617
V +++ T V +EM + L AV GDN
Sbjct: 288 VGYGSHLKTTVTGIEMFKKQLDAAVAGDN 316
>SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 646
Score = 32.7 bits (71), Expect = 0.044
Identities = 26/94 (27%), Positives = 40/94 (42%)
Frame = +3
Query: 318 MPWFKGWQVERKEGKADGKCLIEALDAILPPARPTDKPLRLPLQDVYKIGGIGTVPVGRV 497
+P K V K GK + L E + I PP + PLR L D + G + + R+
Sbjct: 206 IPMSKPILVSSKTGKNVEQILPEIIQKIPPPKGSENAPLRCLLIDSWYNSYQGVIGLVRI 265
Query: 498 ETGVLKPGTIVVFAPANITTEVKSVEMHHEALQE 599
G +K G V+ EV+ V + + + E
Sbjct: 266 MEGFIKKGGKVMSVNTGRKYEVQQVGIMYPDMTE 299
>SPAC1952.11c |ure2||urease |Schizosaccharomyces pombe|chr
1|||Manual
Length = 835
Score = 28.3 bits (60), Expect = 0.95
Identities = 15/46 (32%), Positives = 19/46 (41%)
Frame = -2
Query: 418 GRAGGRMASRASMRHFPSALPSLRSTCHPLNQGILVEGSNMLSPCH 281
G GG S+ P+ LPS + P L E +ML CH
Sbjct: 545 GAGGGHAPDIISLVQNPNILPSSTNPTRPFTTNTLDEELDMLMVCH 590
>SPBC24C6.08c |||vesicle coat protein|Schizosaccharomyces pombe|chr
2|||Manual
Length = 367
Score = 27.1 bits (57), Expect = 2.2
Identities = 15/63 (23%), Positives = 31/63 (49%), Gaps = 3/63 (4%)
Frame = +1
Query: 181 TVSPDLRKSRRKYPHTSRRLATTQLL---SLSCPFLDGTETTCWSLQPKCLGSRDGRWSV 351
+ +PD+ ++PHT +R +T + L LSC + +E + P G +D + +
Sbjct: 145 STNPDVLYMSSQHPHTQQRYSTLKRLMVRCLSCEYSTLSEASDSMSNPLFFGDQDNGYVI 204
Query: 352 RKA 360
++
Sbjct: 205 SQS 207
>SPBC21D10.06c |map4||cell agglutination protein
Map4|Schizosaccharomyces pombe|chr 2|||Manual
Length = 948
Score = 27.1 bits (57), Expect = 2.2
Identities = 14/39 (35%), Positives = 22/39 (56%)
Frame = -2
Query: 562 TSVVMLAGAKTTMVPGFNTPVSTLPTGTVPIPPILYTSC 446
+SVV+ + +T V + + VST TGTV +P +C
Sbjct: 86 SSVVLYSAKETVTVSSYWSLVSTSVTGTVYVPYTSSVAC 124
>SPBC17G9.09 |tif213||translation initiation factor eIF2 gamma
subunit|Schizosaccharomyces pombe|chr 2|||Manual
Length = 446
Score = 26.6 bits (56), Expect = 2.9
Identities = 17/76 (22%), Positives = 32/76 (42%)
Frame = +3
Query: 3 ITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPY 182
+ G + D A+L++A QT EH + +K +I+ NK+D
Sbjct: 126 LNGAAVMDAALLLIAGNES------CPQPQTSEHLAAIEIMQLKHIIILQNKVDLIRESA 179
Query: 183 SEPRFEEIKKEVSSYI 230
+E ++ I K + +
Sbjct: 180 AEEHYQSILKFIKGTV 195
>SPAC23H4.03c |erv25||COPII-coated vesicle component Erv25
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 216
Score = 26.6 bits (56), Expect = 2.9
Identities = 19/74 (25%), Positives = 36/74 (48%)
Frame = -1
Query: 569 RLDFSSDVGGGKDNNGTWFQHTSFNSADGHGTNTTDFVYVLQGKTQGLVSGAGRWQDGIE 390
+L+F+ VG D+ + + + + DF+ ++GK L + R+++ E
Sbjct: 120 KLEFT--VGADADDYSSLQKANNLEPVEADIRRARDFIEEIKGKIYYLQAREARFRNTNE 177
Query: 389 SFNEAFSVSFAFLT 348
S NE +FA+LT
Sbjct: 178 STNERVK-NFAYLT 190
>SPAC222.09 |seb1||RNA-binding protein Seb1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 620
Score = 26.2 bits (55), Expect = 3.8
Identities = 22/72 (30%), Positives = 25/72 (34%), Gaps = 5/72 (6%)
Frame = -2
Query: 520 PGFNTPVSTLPTGTVPIP-PILYTSCR--GRRRGLSVGRAGGRMASRASMRH--FPSALP 356
P +N P G+VP+P P S R R S GR S H PS LP
Sbjct: 314 PNYNAPFQPAGVGSVPLPAPTSSQSLRLGSLHRSRSPSPRSGRPRRSPSPSHLSIPSTLP 373
Query: 355 SLRSTCHPLNQG 320
P G
Sbjct: 374 PADGVPKPTPDG 385
>SPAC6F12.17 |rna14||mRNA cleavage and polyadenylation specificity
factor complex subunit Rna14|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 733
Score = 25.4 bits (53), Expect = 6.7
Identities = 10/18 (55%), Positives = 12/18 (66%)
Frame = -2
Query: 511 NTPVSTLPTGTVPIPPIL 458
N P S LPT VP+P I+
Sbjct: 666 NPPTSALPTVPVPLPSII 683
>SPAC19G12.08 |||fatty acid hydroxylase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 347
Score = 25.4 bits (53), Expect = 6.7
Identities = 18/57 (31%), Positives = 28/57 (49%)
Frame = +3
Query: 159 MDSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLEPSTKMPWF 329
+D +P + F IKK+V Y+ ++ + P + G N LEP TK PW+
Sbjct: 107 LDLKKPLLPQILFGNIKKDV--YLDQV-HRPRHYRGSGSAPLFG-NFLEPLTKTPWY 159
>SPAC10F6.05c |ubc6||ubiquitin conjugating enzyme
Ubc6|Schizosaccharomyces pombe|chr 1|||Manual
Length = 227
Score = 25.0 bits (52), Expect = 8.8
Identities = 13/38 (34%), Positives = 16/38 (42%)
Frame = -2
Query: 598 SWRASWCISTDLTSVVMLAGAKTTMVPGFNTPVSTLPT 485
SW SW +ST L +V + G T ST T
Sbjct: 97 SWNPSWMVSTILVGLVSFMTSDEITTGGIVTSESTRRT 134
>SPAC57A7.11 |mip1||WD repeat protein Mip1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1313
Score = 25.0 bits (52), Expect = 8.8
Identities = 20/62 (32%), Positives = 27/62 (43%)
Frame = +1
Query: 247 TQLLSLSCPFLDGTETTCWSLQPKCLGSRDGRWSVRKAKLTENASLKLSMPSCHLPAPLT 426
T L L+ F T+T W++ PK L R R + A L N L + H P +
Sbjct: 360 TPLGELNWIFTAITDTIAWNVFPKHLFRRLFRQDLMVAALFRNFLLAERIMLVHSCHPQS 419
Query: 427 SP 432
SP
Sbjct: 420 SP 421
>SPBC11B10.05c |rsp1||random septum position protein
Rsp1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 494
Score = 25.0 bits (52), Expect = 8.8
Identities = 15/45 (33%), Positives = 23/45 (51%), Gaps = 3/45 (6%)
Frame = +3
Query: 210 KEVS---SYIKKIGYNPAAVAFVPISGWHGDNMLEPSTKMPWFKG 335
KEVS SYI +P +G+ +N+++P + P FKG
Sbjct: 159 KEVSTSKSYISSGYLHPKTSPIFKKNGYATENVVDPISSSPRFKG 203
>SPBC1539.08 |||ADP-ribosylation factor, Arf
family|Schizosaccharomyces pombe|chr 2|||Manual
Length = 184
Score = 25.0 bits (52), Expect = 8.8
Identities = 14/37 (37%), Positives = 20/37 (54%)
Frame = +1
Query: 277 LDGTETTCWSLQPKCLGSRDGRWSVRKAKLTENASLK 387
LD + W++QP C + DG A L++NA LK
Sbjct: 149 LDKLKDRLWNVQPTCALTGDGLLE-GLAWLSQNAKLK 184
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,799,993
Number of Sequences: 5004
Number of extensions: 61996
Number of successful extensions: 238
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 219
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 230
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 273658928
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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