BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmmt11o08
(700 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC609.02 |ptn1||phosphatidylinositol-3,4,5-trisphosphate3-phos... 30 0.37
SPAC1952.16 |rga9||RhoGAp, GTPase activator towards Rho/Rac/Cdc4... 29 0.85
SPAC29B12.12 |||helper of TIM |Schizosaccharomyces pombe|chr 1||... 28 1.5
SPAC4D7.01c |sec71|sec7a, SPAP8A3.15c|Sec7 domain|Schizosaccharo... 27 2.0
SPCC16C4.06c |||tRNA pseudouridylate synthase |Schizosaccharomyc... 27 2.6
SPBP8B7.12c |fta3|sma3|Sim4 and Mal2 associated |Schizosaccharom... 26 4.5
SPAC1006.09 |win1|SPAC1250.06c, SPAPJ730.01|MAP kinase kinase ki... 26 6.0
SPBC428.20c |alp6|SPBC902.01c|gamma tubulin complex Spc98/GCP3 s... 25 7.9
>SPBC609.02 |ptn1||phosphatidylinositol-3,4,
5-trisphosphate3-phosphatase|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 348
Score = 29.9 bits (64), Expect = 0.37
Identities = 15/47 (31%), Positives = 24/47 (51%)
Frame = -1
Query: 298 GVHRLFSSDELELLTTPTVLRFSNWATLLSKVSRIRRETYFHMITFK 158
G+H+L+ +DEL++ T NW L + ET +H+ FK
Sbjct: 45 GIHKLYRNDELDVFKYLTTQLKDNWILL----NLCAEETVYHLELFK 87
>SPAC1952.16 |rga9||RhoGAp, GTPase activator towards
Rho/Rac/Cdc42-like small GTPases|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 684
Score = 28.7 bits (61), Expect = 0.85
Identities = 12/37 (32%), Positives = 21/37 (56%)
Frame = -1
Query: 247 TVLRFSNWATLLSKVSRIRRETYFHMITFKLIFTCIL 137
T F+N L K S ++R++Y H+ ++L+ C L
Sbjct: 194 TAEEFANVLLKLMKASTVKRKSYSHLGDYELVTNCSL 230
>SPAC29B12.12 |||helper of TIM |Schizosaccharomyces pombe|chr
1|||Manual
Length = 113
Score = 27.9 bits (59), Expect = 1.5
Identities = 20/78 (25%), Positives = 33/78 (42%)
Frame = -2
Query: 471 CGICILYESADLCTSQLLIHCSM*WHQSKFCAQNSIVCCLSRHSYTSSSIYKMKTNEMEY 292
CG C + + C +L H + W ++KF ++C ++S T Y+ T +Y
Sbjct: 37 CGQCEKFYACFQCHDELNTHPFLPWRKAKFHIP-CVICGACKNSLTVEE-YR-STVHCKY 93
Query: 291 IDFFHQMNSNC*QHQQCY 238
+ H N C H Y
Sbjct: 94 CN--HPFNPKCKNHAGYY 109
>SPAC4D7.01c |sec71|sec7a, SPAP8A3.15c|Sec7 domain|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1811
Score = 27.5 bits (58), Expect = 2.0
Identities = 7/31 (22%), Positives = 23/31 (74%)
Frame = +3
Query: 279 EKSLCTPSRWFSSYKCLSSCRNDGTNSKRWN 371
+++LC ++++++C+SS ++D +++ +N
Sbjct: 1689 QEALCAKLYFYTAFECMSSLKSDSHDTEEYN 1719
>SPCC16C4.06c |||tRNA pseudouridylate synthase |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 413
Score = 27.1 bits (57), Expect = 2.6
Identities = 13/33 (39%), Positives = 16/33 (48%)
Frame = -2
Query: 390 SKFCAQNSIVCCLSRHSYTSSSIYKMKTNEMEY 292
S+F A S++C L RH SSI M Y
Sbjct: 8 SRFSANQSVLCLLQRHRVFFSSISVKPRKRMVY 40
>SPBP8B7.12c |fta3|sma3|Sim4 and Mal2 associated
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 220
Score = 26.2 bits (55), Expect = 4.5
Identities = 15/42 (35%), Positives = 25/42 (59%)
Frame = -1
Query: 388 QVLCSKFHRLLFVPSFLHELKHL*DENQRDGVHRLFSSDELE 263
Q L + + L F+ S L+E +HL +EN + F+S+E+E
Sbjct: 89 QRLYDESNSLGFISSPLNETEHLSEENLKIESSITFTSEEIE 130
>SPAC1006.09 |win1|SPAC1250.06c, SPAPJ730.01|MAP kinase kinase
kinase Win1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1436
Score = 25.8 bits (54), Expect = 6.0
Identities = 10/19 (52%), Positives = 13/19 (68%)
Frame = +2
Query: 401 YMEQWINNWLVHKSADSYK 457
+ME W WL H+S DSY+
Sbjct: 361 WMEIWC--WLTHRSVDSYR 377
>SPBC428.20c |alp6|SPBC902.01c|gamma tubulin complex Spc98/GCP3
subunit Alp6|Schizosaccharomyces pombe|chr 2|||Manual
Length = 821
Score = 25.4 bits (53), Expect = 7.9
Identities = 9/33 (27%), Positives = 18/33 (54%)
Frame = +3
Query: 534 FICSINNGFHQTSPVRIEKIISTFTPSVHSISN 632
F CS++N P+ + + + +TP + +SN
Sbjct: 738 FSCSLSNRIRINVPISTDALAAQYTPIKNELSN 770
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,672,798
Number of Sequences: 5004
Number of extensions: 54728
Number of successful extensions: 145
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 142
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 145
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 323158234
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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