BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= bmmt10l15 (350 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC1393.04 |fta4|sma6|Sim4 and Mal2 associated |Schizosaccharom... 29 0.21 SPBC3D6.04c |mad1||mitotic spindle checkpoint protein Mad1|Schiz... 27 0.64 SPBC29A10.04 |psm1|smc1|mitotic cohesin complex subunit Psm1 |Sc... 27 0.64 SPAC29E6.03c |uso1|SPAC30.07c|ER to Golgi tethering factor Uso1 ... 27 1.1 SPAC1486.03c |||RNA-binding splicing factor|Schizosaccharomyces ... 26 1.5 SPAC1783.05 |hrp1|chd1|ATP-dependent DNA helicase Hrp1|Schizosac... 25 2.6 SPCP31B10.05 |||tyrosyl-DNA phosphodiesterase |Schizosaccharomyc... 25 2.6 SPBC14C8.14c |pol5||DNA polymerase phi|Schizosaccharomyces pombe... 25 2.6 SPAC11E3.03 |pcs1||chromosome segregation protein Pcs1 |Schizosa... 25 3.4 SPBC1861.04c |||RNA-binding protein Prp24|Schizosaccharomyces po... 25 3.4 SPAPB1A10.06c |||ATP-dependent RNA helicase Dhr1 |Schizosaccharo... 25 3.4 SPAC22F8.11 |plc1||phosphoinositide phospholipase C Plc1|Schizos... 24 6.0 SPAC24H6.13 |||DUF221 family protein|Schizosaccharomyces pombe|c... 24 6.0 SPAC3A11.06 |mvp1||sorting nexin Mvp1|Schizosaccharomyces pombe|... 24 6.0 SPAC1F5.11c |||phosphatidylinositol kinase |Schizosaccharomyces ... 24 6.0 SPAC694.06c |mrc1||mediator of replication checkpoint 1 |Schizos... 24 6.0 SPCC18B5.07c |nup61||nucleoporin Nup61|Schizosaccharomyces pombe... 24 7.9 >SPCC1393.04 |fta4|sma6|Sim4 and Mal2 associated |Schizosaccharomyces pombe|chr 3|||Manual Length = 233 Score = 29.1 bits (62), Expect = 0.21 Identities = 19/61 (31%), Positives = 31/61 (50%), Gaps = 1/61 (1%) Frame = +2 Query: 47 ISTPLTKKNPEVFNKTMNNYSALANKKIKLL-DAQIDNLNKKQEQEVKEHELRCTLLQLE 223 I T + KKNPE+ + T+N+ + + K LL +LN ++ E E+R L + Sbjct: 171 IHTNMWKKNPELIS-TLNSTNTMVAKINSLLRGVSFPSLNNDNQEASLEDEIRSQLFEKT 229 Query: 224 I 226 I Sbjct: 230 I 230 >SPBC3D6.04c |mad1||mitotic spindle checkpoint protein Mad1|Schizosaccharomyces pombe|chr 2|||Manual Length = 689 Score = 27.5 bits (58), Expect = 0.64 Identities = 20/74 (27%), Positives = 37/74 (50%) Frame = +2 Query: 14 PKSSNFLLKRKISTPLTKKNPEVFNKTMNNYSALANKKIKLLDAQIDNLNKKQEQEVKEH 193 P S L R ++T + K P + ++N+ + N K+ L+ Q++NL + ++K Sbjct: 21 PFQSRSQLPRFLATSV--KKPNLKKPSVNSANETKNPKLASLEFQLENL----KNDLKRK 74 Query: 194 ELRCTLLQLEIDIK 235 EL Q+E+ K Sbjct: 75 ELEFEREQIELQRK 88 >SPBC29A10.04 |psm1|smc1|mitotic cohesin complex subunit Psm1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 1233 Score = 27.5 bits (58), Expect = 0.64 Identities = 15/58 (25%), Positives = 31/58 (53%) Frame = +2 Query: 77 EVFNKTMNNYSALANKKIKLLDAQIDNLNKKQEQEVKEHELRCTLLQLEIDIKKKVLD 250 E+F+KT L + K+L+A D + +Q+ K+ E L ++ ++K +++D Sbjct: 473 ELFHKTQELNEELQSCLQKILEASAD--RNESKQDAKKREALYALKRIYPEVKGRIID 528 >SPAC29E6.03c |uso1|SPAC30.07c|ER to Golgi tethering factor Uso1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1044 Score = 26.6 bits (56), Expect = 1.1 Identities = 19/74 (25%), Positives = 35/74 (47%), Gaps = 4/74 (5%) Frame = +2 Query: 35 LKRKISTPLTKKNP--EVFNKTMNNYSALANKKIKLLD--AQIDNLNKKQEQEVKEHELR 202 LK K+S T+ N+ N L + D + ++++NK Q E+K + + Sbjct: 804 LKNKLSEESTRLQELQSQLNQDKNQIETLNERISAAADELSSMESINKNQANELKLAKQK 863 Query: 203 CTLLQLEIDIKKKV 244 C+ LQ +I+ K+ Sbjct: 864 CSNLQEKINFGNKL 877 >SPAC1486.03c |||RNA-binding splicing factor|Schizosaccharomyces pombe|chr 1|||Manual Length = 797 Score = 26.2 bits (55), Expect = 1.5 Identities = 12/37 (32%), Positives = 22/37 (59%) Frame = +2 Query: 179 EVKEHELRCTLLQLEIDIKKKVLDSFN*LIKCVVIKY 289 EVK + T+ + EID+ +K L N LI+ + +++ Sbjct: 312 EVKSRSMEFTVPEAEIDVIEKRLQPLNNLIETLPVEF 348 >SPAC1783.05 |hrp1|chd1|ATP-dependent DNA helicase Hrp1|Schizosaccharomyces pombe|chr 1|||Manual Length = 1373 Score = 25.4 bits (53), Expect = 2.6 Identities = 11/42 (26%), Positives = 23/42 (54%), Gaps = 1/42 (2%) Frame = +2 Query: 71 NPEVFNKTMNNYSALAN-KKIKLLDAQIDNLNKKQEQEVKEH 193 N +M +SA+ + LDA++DN++ ++ +E +H Sbjct: 1294 NKRTTRNSMKTHSAMDTLTAVAALDAELDNMSNEKAKEEVDH 1335 >SPCP31B10.05 |||tyrosyl-DNA phosphodiesterase |Schizosaccharomyces pombe|chr 3|||Manual Length = 536 Score = 25.4 bits (53), Expect = 2.6 Identities = 10/29 (34%), Positives = 18/29 (62%) Frame = -2 Query: 316 QLQQFTITYIFNYNAFYQLIKTIEYFFFN 230 Q QQ + Y+ NY Y+LI+ ++ + F+ Sbjct: 229 QFQQDFLAYLKNYRHTYELIEKLKMYDFS 257 >SPBC14C8.14c |pol5||DNA polymerase phi|Schizosaccharomyces pombe|chr 2|||Manual Length = 959 Score = 25.4 bits (53), Expect = 2.6 Identities = 17/60 (28%), Positives = 25/60 (41%) Frame = +2 Query: 71 NPEVFNKTMNNYSALANKKIKLLDAQIDNLNKKQEQEVKEHELRCTLLQLEIDIKKKVLD 250 N E + K+K++D IDN K Q E LL++ + K KVL+ Sbjct: 772 NKEKKKNAQETKQQIVQFKVKVIDL-IDNYYKTQPNNGLGFEFLIPLLEMILKTKHKVLE 830 >SPAC11E3.03 |pcs1||chromosome segregation protein Pcs1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 222 Score = 25.0 bits (52), Expect = 3.4 Identities = 10/24 (41%), Positives = 16/24 (66%) Frame = +2 Query: 119 NKKIKLLDAQIDNLNKKQEQEVKE 190 NKK++L + ++ L+ QEQE E Sbjct: 36 NKKLQLPNVELSTLSHTQEQEFNE 59 >SPBC1861.04c |||RNA-binding protein Prp24|Schizosaccharomyces pombe|chr 2|||Manual Length = 1014 Score = 25.0 bits (52), Expect = 3.4 Identities = 9/27 (33%), Positives = 20/27 (74%) Frame = -2 Query: 325 LITQLQQFTITYIFNYNAFYQLIKTIE 245 L+ +L +FTI + +NY++ +LI+ ++ Sbjct: 28 LLDELTKFTILHPYNYDSHIKLIEELK 54 >SPAPB1A10.06c |||ATP-dependent RNA helicase Dhr1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1183 Score = 25.0 bits (52), Expect = 3.4 Identities = 13/38 (34%), Positives = 24/38 (63%), Gaps = 1/38 (2%) Frame = +2 Query: 65 KKNPEVFNKTMN-NYSALANKKIKLLDAQIDNLNKKQE 175 K+ E+ ++ + N ++++KK K LD I+N KK+E Sbjct: 67 KRKQELKDQLLKENEGSISSKKKKRLDKYIENKLKKEE 104 >SPAC22F8.11 |plc1||phosphoinositide phospholipase C Plc1|Schizosaccharomyces pombe|chr 1|||Manual Length = 899 Score = 24.2 bits (50), Expect = 6.0 Identities = 10/29 (34%), Positives = 16/29 (55%) Frame = +2 Query: 86 NKTMNNYSALANKKIKLLDAQIDNLNKKQ 172 N +MN YS +N+ + L + N N +Q Sbjct: 127 NTSMNPYSCDSNENLSTLSSVNQNFNSRQ 155 >SPAC24H6.13 |||DUF221 family protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 871 Score = 24.2 bits (50), Expect = 6.0 Identities = 15/51 (29%), Positives = 25/51 (49%) Frame = +2 Query: 89 KTMNNYSALANKKIKLLDAQIDNLNKKQEQEVKEHELRCTLLQLEIDIKKK 241 K + S L NK L++ I+ KK + VK+H+ + L +KK+ Sbjct: 223 KKVKKRSDLGNKYESTLNSLINKSVKKHNKLVKKHKPLPSTLDYTAYVKKR 273 >SPAC3A11.06 |mvp1||sorting nexin Mvp1|Schizosaccharomyces pombe|chr 1|||Manual Length = 664 Score = 24.2 bits (50), Expect = 6.0 Identities = 18/79 (22%), Positives = 36/79 (45%), Gaps = 1/79 (1%) Frame = +2 Query: 14 PKSSNFLLKRKISTPLTKK-NPEVFNKTMNNYSALANKKIKLLDAQIDNLNKKQEQEVKE 190 P + + K++ P+ ++ NPE+FN++ +N + L NL+ + E Sbjct: 107 PSTLEQIFKQRNVLPILQRFNPELFNRSSDNETPLFPNNSPPASTTALNLSSNIVPSINE 166 Query: 191 HELRCTLLQLEIDIKKKVL 247 ++ L Q + D+ K L Sbjct: 167 SKI---LEQEDDDVSNKSL 182 >SPAC1F5.11c |||phosphatidylinositol kinase |Schizosaccharomyces pombe|chr 1|||Manual Length = 3655 Score = 24.2 bits (50), Expect = 6.0 Identities = 15/53 (28%), Positives = 26/53 (49%) Frame = +2 Query: 32 LLKRKISTPLTKKNPEVFNKTMNNYSALANKKIKLLDAQIDNLNKKQEQEVKE 190 ++ R IS P P+ ++ + + NK +L D+ KK E+E+KE Sbjct: 423 MVDRMISLPSI---PDAIFLLLSIFDSFVNKFSELNDSLDQFFKKKYEEEIKE 472 >SPAC694.06c |mrc1||mediator of replication checkpoint 1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1019 Score = 24.2 bits (50), Expect = 6.0 Identities = 13/39 (33%), Positives = 21/39 (53%), Gaps = 1/39 (2%) Frame = +2 Query: 125 KIKLLDAQIDNLNKKQEQEVKEHELRCTLLQL-EIDIKK 238 ++K +DN+ K + E EH ++ L QL EI + K Sbjct: 358 EVKPKTTALDNIEKSKLSEENEHGIKGKLKQLAEIKLSK 396 >SPCC18B5.07c |nup61||nucleoporin Nup61|Schizosaccharomyces pombe|chr 3|||Manual Length = 565 Score = 23.8 bits (49), Expect = 7.9 Identities = 14/45 (31%), Positives = 24/45 (53%), Gaps = 2/45 (4%) Frame = +2 Query: 8 ETPK--SSNFLLKRKISTPLTKKNPEVFNKTMNNYSALANKKIKL 136 E PK S++ + RKI+ P ++K P + ++ LA K + L Sbjct: 26 EVPKEASADVMATRKIAKPKSRKRPTSGVSSPGIFANLAAKPVSL 70 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,197,116 Number of Sequences: 5004 Number of extensions: 21089 Number of successful extensions: 92 Number of sequences better than 10.0: 17 Number of HSP's better than 10.0 without gapping: 90 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 92 length of database: 2,362,478 effective HSP length: 64 effective length of database: 2,042,222 effective search space used: 106195544 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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