Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmmt10k20
(687 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g06530.1 68414.m00692 myosin heavy chain-related similar to m... 37 0.014
At4g38070.1 68417.m05377 bHLH family protein contains Pfam profi... 35 0.058
At2g38720.1 68415.m04755 microtubule associated protein (MAP65/A... 34 0.10
At1g37020.1 68414.m04616 Ulp1 protease family protein 31 0.54
At3g19050.1 68416.m02420 kinesin motor protein-related contains ... 31 0.72
At1g59453.1 68414.m06679 transcription factor-related weak simil... 31 0.72
At5g66030.2 68418.m08315 Golgi-localized GRIP domain-containing ... 30 1.7
At5g66030.1 68418.m08314 Golgi-localized GRIP domain-containing ... 30 1.7
At2g46180.1 68415.m05742 intracellular protein transport protein... 30 1.7
At1g55255.1 68414.m06311 zinc finger (C3HC4-type RING finger) fa... 30 1.7
At1g34320.1 68414.m04259 expressed protein contains Pfam domain ... 30 1.7
At5g48310.1 68418.m05968 expressed protein 29 2.2
At4g26050.1 68417.m03750 leucine-rich repeat family protein cont... 29 2.2
At1g02420.1 68414.m00189 pentatricopeptide (PPR) repeat-containi... 29 2.2
At4g32700.1 68417.m04655 DNA-directed DNA polymerase family prot... 29 2.9
At2g35110.1 68415.m04307 HEM protein-related weak similarity to ... 29 2.9
At1g19230.1 68414.m02393 respiratory burst oxidase protein E (Rb... 29 2.9
At1g02330.1 68414.m00178 expressed protein contains similarity t... 29 2.9
At4g10270.1 68417.m01688 wound-responsive family protein similar... 29 3.8
At1g78270.1 68414.m09121 UDP-glucose glucosyltransferase, putati... 28 5.0
At1g64170.1 68414.m07269 cation/hydrogen exchanger, putative (CH... 28 5.0
At1g53720.1 68414.m06113 cyclophilin-RNA interacting protein, pu... 28 5.0
At1g33970.1 68414.m04212 avirulence-responsive protein, putative... 28 6.7
At5g46860.1 68418.m05774 syntaxin 22 (SYP22) (VAM3) identical to... 27 8.8
At3g49010.2 68416.m05354 60S ribosomal protein L13 (RPL13B) / br... 27 8.8
At3g49010.1 68416.m05353 60S ribosomal protein L13 (RPL13B) / br... 27 8.8
>At1g06530.1 68414.m00692 myosin heavy chain-related similar to
myosin heavy chain (GI:1408194) {Placopecten
magellanicus}; similar to Myosin heavy chain, clone 203
(Fragment) (SP:P39922){Hydra attenuata}; contains one
transmembrane domain
Length = 323
Score = 36.7 bits (81), Expect = 0.014
Identities = 24/93 (25%), Positives = 45/93 (48%), Gaps = 3/93 (3%)
Frame = +2
Query: 299 QADQLRAEWSRE---LARVEDEIATLRTVLQSKIRQSSDLKRKLGITVWKEITEDVNQGL 469
+A++LR+E S++ + +E E+A LRTV + ++ +L+ KLG KE+ E +
Sbjct: 131 EAEKLRSEISQKGGGIEELEKEVAGLRTVKEENEKRMKELESKLGALEVKELDEKNKKFR 190
Query: 470 KNVKESQVYQKTESVIKTTAEKTSSIIGGITAG 568
+ + E + EK S+ + G
Sbjct: 191 AEEEMREKIDNKEKEVHDLKEKIKSLESDVAKG 223
>At4g38070.1 68417.m05377 bHLH family protein contains Pfam profile:
PF00010 helix-loop-helix DNA-binding domain; PMID:
12679534; putative bHLH131 transcription factor
Length = 1513
Score = 34.7 bits (76), Expect = 0.058
Identities = 16/36 (44%), Positives = 24/36 (66%)
Frame = +2
Query: 314 RAEWSRELARVEDEIATLRTVLQSKIRQSSDLKRKL 421
+ EW E +++ DEI +L+T L S R S DL++KL
Sbjct: 211 KKEWEEEKSKLLDEIYSLQTKLDSVTRISEDLQKKL 246
>At2g38720.1 68415.m04755 microtubule associated protein
(MAP65/ASE1) family protein low similarity to myosin
[Schistosoma japonicum] GI:3941320; contains Pfam
profile PF03999: Microtubule associated protein
(MAP65/ASE1 family)
Length = 587
Score = 33.9 bits (74), Expect = 0.10
Identities = 29/88 (32%), Positives = 45/88 (51%), Gaps = 2/88 (2%)
Frame = +2
Query: 296 EQADQLRAEWSRELARVEDEIATLRTVLQSKIRQSSDLKRKLGITVWKEITEDVNQGLKN 475
E+ + RAE R LA+ E EIA+L + L K+ S K+K G KE V L++
Sbjct: 50 EKTRKFRAELQRSLAQAEAEIASLMSALGEKV---SFAKKKEGSL--KEQISSVKPVLED 104
Query: 476 --VKESQVYQKTESVIKTTAEKTSSIIG 553
+K+ + ++ + AE TS+I G
Sbjct: 105 LLMKKDRRRKELSETLNQIAEITSNIAG 132
>At1g37020.1 68414.m04616 Ulp1 protease family protein
Length = 611
Score = 31.5 bits (68), Expect = 0.54
Identities = 21/75 (28%), Positives = 36/75 (48%)
Frame = +2
Query: 347 EDEIATLRTVLQSKIRQSSDLKRKLGITVWKEITEDVNQGLKNVKESQVYQKTESVIKTT 526
EDE LQ KI+ ++ K+K + K+ ++ + + VKE +V T V+K T
Sbjct: 253 EDETPNKELELQLKIKDATKGKKKKVAIMPKKASKIPKKKMAKVKEMKV--STPKVLKKT 310
Query: 527 AEKTSSIIGGITAGV 571
+ +G +T V
Sbjct: 311 MRRDDDYVGDVTEKV 325
>At3g19050.1 68416.m02420 kinesin motor protein-related contains Pfam
profile: PF00225 Kinesin motor domain; contains
non-consensus splice site (GC) at intron 12
Length = 2722
Score = 31.1 bits (67), Expect = 0.72
Identities = 27/92 (29%), Positives = 44/92 (47%), Gaps = 1/92 (1%)
Frame = +2
Query: 215 QTAEEAMS-GTGVAGDIHTPDELAGLTPEQADQLRAEWSRELARVEDEIATLRTVLQSKI 391
Q + ++S G+G +P GL Q Q+R+E ELA I L TV+ ++
Sbjct: 2385 QAIDSSLSKGSGKPRGSGSPFRCIGLGITQ--QMRSEKDEELAAARLRIEELETVVSTRQ 2442
Query: 392 RQSSDLKRKLGITVWKEITEDVNQGLKNVKES 487
++ L KL +T D+N+ L VK++
Sbjct: 2443 KEIFLLNSKLAKV--DSMTHDINRVLLGVKQN 2472
>At1g59453.1 68414.m06679 transcription factor-related weak similarity
to TFIIIC Box B-binding subunit [Homo sapiens] GI:442362
Length = 1729
Score = 31.1 bits (67), Expect = 0.72
Identities = 19/59 (32%), Positives = 30/59 (50%)
Frame = +2
Query: 437 KEITEDVNQGLKNVKESQVYQKTESVIKTTAEKTSSIIGGITAGVSSKLGQMRNSESFR 613
K ++D N+ ++N S +K S++KT E SII ++S NSESF+
Sbjct: 907 KSNSKDKNKAVENSPSSSKKRKRASLVKTKGEGVKSIIVDGQKVLNSDAIDASNSESFQ 965
>At5g66030.2 68418.m08315 Golgi-localized GRIP domain-containing
protein contains Pfam profile PF01465: GRIP domain;
supporting cDNA gi|20303028|gb|AF499634.1|
Length = 765
Score = 29.9 bits (64), Expect = 1.7
Identities = 14/31 (45%), Positives = 21/31 (67%)
Frame = +2
Query: 296 EQADQLRAEWSRELARVEDEIATLRTVLQSK 388
E AD+L E RE++R+ DE+ LR ++SK
Sbjct: 585 ELADRLIEEKDREISRLVDEMTNLRKSMESK 615
>At5g66030.1 68418.m08314 Golgi-localized GRIP domain-containing
protein contains Pfam profile PF01465: GRIP domain;
supporting cDNA gi|20303028|gb|AF499634.1|
Length = 788
Score = 29.9 bits (64), Expect = 1.7
Identities = 14/31 (45%), Positives = 21/31 (67%)
Frame = +2
Query: 296 EQADQLRAEWSRELARVEDEIATLRTVLQSK 388
E AD+L E RE++R+ DE+ LR ++SK
Sbjct: 585 ELADRLIEEKDREISRLVDEMTNLRKSMESK 615
>At2g46180.1 68415.m05742 intracellular protein transport protein
USO1-related similar to Intracellular protein transport
protein USO1 (Swiss-Prot:P25386) [Saccharomyces
cerevisiae]
Length = 725
Score = 29.9 bits (64), Expect = 1.7
Identities = 11/30 (36%), Positives = 20/30 (66%)
Frame = +2
Query: 302 ADQLRAEWSRELARVEDEIATLRTVLQSKI 391
A+ + AEW +++VED+ A +R VL+ +
Sbjct: 528 AENIAAEWKNRVSKVEDDNAKVRRVLEQSM 557
>At1g55255.1 68414.m06311 zinc finger (C3HC4-type RING finger)
family protein contains Pfam profile: PF00097 zinc
finger, C3HC4 type (RING finger)
Length = 383
Score = 29.9 bits (64), Expect = 1.7
Identities = 30/132 (22%), Positives = 62/132 (46%), Gaps = 5/132 (3%)
Frame = +2
Query: 287 LTPEQADQLRAEWSRELARVEDEIATLRTVLQSKIRQSSDLKRKLGITVWKEITEDVNQG 466
L + A + + LA + EIA LRT L R+ +LK GI V ++ E
Sbjct: 84 LRVKAAHETESACQERLATAKAEIAELRTQLDLSEREVLELKE--GIKVKEQEAEASIAE 141
Query: 467 LKNVKESQVYQKTES--VIKTTAEKTSSIIGGITAGVSSKLGQMRNSESFRSIEE---RV 631
++ + ++ +T++ +++ AE+ I ++ V +K + + +E+ +V
Sbjct: 142 METIGQAYEDMQTQNQHLLQQVAERDDYNIKLVSESVKTKHAYNTHLSEKQVMEKQLHQV 201
Query: 632 GSAYENVKGKVA 667
++ EN K ++A
Sbjct: 202 NASVENFKARIA 213
>At1g34320.1 68414.m04259 expressed protein contains Pfam domain
PF05003: protein of unknown function (DUF668)
Length = 657
Score = 29.9 bits (64), Expect = 1.7
Identities = 17/45 (37%), Positives = 28/45 (62%)
Frame = +2
Query: 344 VEDEIATLRTVLQSKIRQSSDLKRKLGITVWKEITEDVNQGLKNV 478
V D +A LRT L+S+ + +LK+K ++W I E+V + L +V
Sbjct: 308 VGDTLAILRTELKSQKKHVRNLKKK---SLWSRILEEVMEKLVDV 349
>At5g48310.1 68418.m05968 expressed protein
Length = 1156
Score = 29.5 bits (63), Expect = 2.2
Identities = 18/56 (32%), Positives = 28/56 (50%), Gaps = 3/56 (5%)
Frame = +2
Query: 257 DIHTPDELAGLTPEQADQLRAEWSRELARVEDEI---ATLRTVLQSKIRQSSDLKR 415
DI TP + Q D + E +++ ++EDEI A + + Q +R SS L R
Sbjct: 159 DIRTPSAPPIMESGQEDSISLEIEKDIQKIEDEICGEAGVESSKQESMRSSSHLYR 214
>At4g26050.1 68417.m03750 leucine-rich repeat family protein
contains Pfam PF00560: Leucine Rich Repeat domains;
Length = 383
Score = 29.5 bits (63), Expect = 2.2
Identities = 25/76 (32%), Positives = 36/76 (47%), Gaps = 1/76 (1%)
Frame = +2
Query: 449 EDVNQGLKNVKESQVYQKTESVIKTTAEKTSSIIGGITA-GVSSKLGQMRNSESFRSIEE 625
E V QGL+ +K+ + TES KT +K S IG + G+SS G+ E +
Sbjct: 286 EVVEQGLEALKQYMSEKMTESYKKTPTKKKSWGIGKLVKYGLSSSPGRSTGREDGKEGFI 345
Query: 626 RVGSAYENVKGKVASR 673
V S Y + G + R
Sbjct: 346 NV-SDYRQIDGIASPR 360
>At1g02420.1 68414.m00189 pentatricopeptide (PPR) repeat-containing
protein low similarity to fertility restorer [Petunia x
hybrida] GI:22128587; contains Pfam profile: PF01535 PPR
repeat
Length = 491
Score = 29.5 bits (63), Expect = 2.2
Identities = 19/63 (30%), Positives = 29/63 (46%)
Frame = +3
Query: 210 VFKQLKKQCQAPEWQGTYILLMSWPASHPSRQTSYALNGVAS*PVSKMKSRL*ELYCKAR 389
V+ LK Q Q P+ Q ILL W +S + + G P + L ++YCK R
Sbjct: 202 VYHSLKHQFQ-PDLQTFNILLSGWKSSEEAEAFFEEMKGKGLKPDVVTYNSLIDVYCKDR 260
Query: 390 SVK 398
++
Sbjct: 261 EIE 263
>At4g32700.1 68417.m04655 DNA-directed DNA polymerase family protein
similar to DNA helicase HEL308 [Homo sapiens]
GI:19110782; contains Pfam profiles PF00271: Helicase
conserved C-terminal domain, PF00176: SNF2 family
N-terminal domain, PF00476: DNA polymerase family A
Length = 1548
Score = 29.1 bits (62), Expect = 2.9
Identities = 14/36 (38%), Positives = 20/36 (55%)
Frame = +2
Query: 530 EKTSSIIGGITAGVSSKLGQMRNSESFRSIEERVGS 637
EK S ++G T GVSS++ +FR I VG+
Sbjct: 802 EKPSEVLGDATLGVSSEINLTSRLPNFRPIGTAVGT 837
>At2g35110.1 68415.m04307 HEM protein-related weak similarity to
Membrane-associated protein Hem (Dhem-2)
(Swiss-Prot:P55162) [Drosophila melanogaster]; weak
similarity to Nck-associated protein 1 (NAP 1)
(p125Nap1) (Membrane-associated protein HEM-2)
(Swiss-Prot:P55161) [Rattus norvegicus]
Length = 1339
Score = 29.1 bits (62), Expect = 2.9
Identities = 11/27 (40%), Positives = 16/27 (59%)
Frame = -1
Query: 573 DTPAVMPPMIEDVFSAVVFITDSVFWY 493
D P+++ P I+ VFSA+ V WY
Sbjct: 417 DQPSLLAPNIQMVFSALALAQSEVLWY 443
>At1g19230.1 68414.m02393 respiratory burst oxidase protein E
(RbohE) / NADPH oxidase nearly identical to respiratory
burst oxidase protein E GI:3242787 [gi:3242787] from
[Arabidopsis thaliana]
Length = 926
Score = 29.1 bits (62), Expect = 2.9
Identities = 14/25 (56%), Positives = 16/25 (64%)
Frame = +3
Query: 165 HSFHTVRVFHCKI*AVFKQLKKQCQ 239
H TV VF+C I V K+LKKQ Q
Sbjct: 885 HPNSTVGVFYCGIQTVAKELKKQAQ 909
>At1g02330.1 68414.m00178 expressed protein contains similarity to
hepatocellular carcinoma-associated antigen 59
GI:7158847 from [Homo sapiens]
Length = 279
Score = 29.1 bits (62), Expect = 2.9
Identities = 19/61 (31%), Positives = 32/61 (52%)
Frame = +2
Query: 320 EWSRELARVEDEIATLRTVLQSKIRQSSDLKRKLGITVWKEITEDVNQGLKNVKESQVYQ 499
E EL RVEDE+ + L+ K ++SS+ T E+ + LKN++E++ +
Sbjct: 122 EVENELKRVEDELYKIPDHLKVK-KRSSEESSTQWTTGIAEVQLPIEYKLKNIEETEAAK 180
Query: 500 K 502
K
Sbjct: 181 K 181
>At4g10270.1 68417.m01688 wound-responsive family protein similar to
wound induced protein (GI:19320) [Lycopersicon
esculentum]
Length = 90
Score = 28.7 bits (61), Expect = 3.8
Identities = 18/46 (39%), Positives = 22/46 (47%)
Frame = +2
Query: 407 LKRKLGITVWKEITEDVNQGLKNVKESQVYQKTESVIKTTAEKTSS 544
LK +LG+ W I VNQ L+N S K S +A TSS
Sbjct: 20 LKDQLGLCRWNYILRSVNQHLRNNVRSVSQGKRFSSSSVSAAVTSS 65
>At1g78270.1 68414.m09121 UDP-glucose glucosyltransferase, putative
similar to UDP-glucose glucosyltransferase GI:3928543
from [Arabidopsis thaliana]
Length = 489
Score = 28.3 bits (60), Expect = 5.0
Identities = 15/58 (25%), Positives = 33/58 (56%)
Frame = +2
Query: 386 KIRQSSDLKRKLGITVWKEITEDVNQGLKNVKESQVYQKTESVIKTTAEKTSSIIGGI 559
+I ++S++ RKLG+ +W+E TE ++ +++ +Y S+ T+E+ G+
Sbjct: 265 EIDKNSEI-RKLGLNLWEEETESLDWLDTKAEKAVIYVNFGSLTVLTSEQILEFAWGL 321
>At1g64170.1 68414.m07269 cation/hydrogen exchanger, putative
(CHX16) monovalent cation:proton antiporter family 2
(CPA2) member, PMID:11500563
Length = 847
Score = 28.3 bits (60), Expect = 5.0
Identities = 14/40 (35%), Positives = 24/40 (60%), Gaps = 1/40 (2%)
Frame = -2
Query: 422 QVYVLSPSFD-GSCFAVQFSKSRFHLRHGLARDSIQRVTG 306
++ V PS G ++ +F+K L++ L RDS+Q +TG
Sbjct: 71 EIIVSPPSTGLGQSYSFRFNKYPTRLKYELYRDSVQLITG 110
>At1g53720.1 68414.m06113 cyclophilin-RNA interacting protein,
putative
Length = 506
Score = 28.3 bits (60), Expect = 5.0
Identities = 22/70 (31%), Positives = 30/70 (42%), Gaps = 3/70 (4%)
Frame = +1
Query: 433 VERDHRGRQS--GFEKRQRKPSIPKN*ICDKNDRRE-DVFDHRRHHGRRVQQARSDAQLG 603
+ER HR R+ E R+ + DK RRE D DHR H + ++ D + G
Sbjct: 437 LERKHRERKERESREDEDRRRRRRREESRDKESRRERDEDDHRSHRDYKERRRERDDRHG 496
Query: 604 VFPLHRRTRR 633
H R R
Sbjct: 497 REARHERRDR 506
>At1g33970.1 68414.m04212 avirulence-responsive protein, putative /
avirulence induced gene protein, putative / AIG protein,
putative similar to SP|P54120 AIG1 protein {Arabidopsis
thaliana}; contains Pfam profile PF04548: AIG1 family
Length = 342
Score = 27.9 bits (59), Expect = 6.7
Identities = 19/48 (39%), Positives = 29/48 (60%), Gaps = 2/48 (4%)
Frame = +2
Query: 287 LTPEQADQLRAEW-SRELA-RVEDEIATLRTVLQSKIRQSSDLKRKLG 424
L EQA +L AE + E+ R DEI LR L+ +++ +L++KLG
Sbjct: 290 LGEEQAARLEAEKRANEVQKRSSDEIKKLRENLERAEKETKELQKKLG 337
>At5g46860.1 68418.m05774 syntaxin 22 (SYP22) (VAM3) identical to
GP|8809669| syntaxin related protein AtVam3p
[Arabidopsis thaliana]
Length = 268
Score = 27.5 bits (58), Expect = 8.8
Identities = 17/62 (27%), Positives = 36/62 (58%), Gaps = 3/62 (4%)
Frame = +2
Query: 293 PEQADQLRAEWSRELARVEDEIATLRTVLQSKIRQSSDLKRKLGIT--VWKEITEDVN-Q 463
PEQ QL+ +EL +++EIA V++ + + ++ +++G ++K++ VN Q
Sbjct: 150 PEQRAQLQESKRQELVLLDNEIAFNEAVIEEREQGIQEIHQQIGEVNEIFKDLAVLVNDQ 209
Query: 464 GL 469
G+
Sbjct: 210 GV 211
>At3g49010.2 68416.m05354 60S ribosomal protein L13 (RPL13B) /
breast basic conserved protein 1-related (BBC1)
Length = 206
Score = 27.5 bits (58), Expect = 8.8
Identities = 22/61 (36%), Positives = 30/61 (49%), Gaps = 3/61 (4%)
Frame = +2
Query: 251 AGDIHTPDELAGLTPEQADQL---RAEWSRELARVEDEIATLRTVLQSKIRQSSDLKRKL 421
AGD TP+ELA T Q D L R + + EL ++ E+ + + KIR KR
Sbjct: 134 AGD-STPEELANATQVQGDYLPIVREKPTMELVKLTSEMKSFKAF--DKIRLERTNKRHA 190
Query: 422 G 424
G
Sbjct: 191 G 191
>At3g49010.1 68416.m05353 60S ribosomal protein L13 (RPL13B) /
breast basic conserved protein 1-related (BBC1)
Length = 206
Score = 27.5 bits (58), Expect = 8.8
Identities = 22/61 (36%), Positives = 30/61 (49%), Gaps = 3/61 (4%)
Frame = +2
Query: 251 AGDIHTPDELAGLTPEQADQL---RAEWSRELARVEDEIATLRTVLQSKIRQSSDLKRKL 421
AGD TP+ELA T Q D L R + + EL ++ E+ + + KIR KR
Sbjct: 134 AGD-STPEELANATQVQGDYLPIVREKPTMELVKLTSEMKSFKAF--DKIRLERTNKRHA 190
Query: 422 G 424
G
Sbjct: 191 G 191
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,356,866
Number of Sequences: 28952
Number of extensions: 256592
Number of successful extensions: 822
Number of sequences better than 10.0: 26
Number of HSP's better than 10.0 without gapping: 803
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 822
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1457719448
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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