BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= bmmt10j24 (676 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC6F6.01 |||VIC sodium channel |Schizosaccharomyces pombe|chr ... 25 7.6 SPBC354.14c |vac8||vacuolar protein Vac8|Schizosaccharomyces pom... 25 10.0 SPBC216.05 |rad3||ATR checkpoint kinase|Schizosaccharomyces pomb... 25 10.0 >SPAC6F6.01 |||VIC sodium channel |Schizosaccharomyces pombe|chr 1|||Manual Length = 1854 Score = 25.4 bits (53), Expect = 7.6 Identities = 14/40 (35%), Positives = 25/40 (62%) Frame = -1 Query: 592 TEYWKINYILSFR*IEKLLKFYKLFTLHISIVIRKSFSKH 473 ++Y + Y+LS +EK ++ KL L + +IRK+F +H Sbjct: 1663 SDYIRRAYVLSE--LEKSIRMEKLLGLVETSIIRKTFLQH 1700 >SPBC354.14c |vac8||vacuolar protein Vac8|Schizosaccharomyces pombe|chr 2|||Manual Length = 550 Score = 25.0 bits (52), Expect = 10.0 Identities = 12/35 (34%), Positives = 18/35 (51%) Frame = +3 Query: 132 KCYALGTHRPLLGFDANNKRIIVINEFIHRCRLII 236 +C+A+ T R L NKR I+ I + R +I Sbjct: 351 QCHAVSTLRNLAASSERNKRAIIEANAIQKLRCLI 385 >SPBC216.05 |rad3||ATR checkpoint kinase|Schizosaccharomyces pombe|chr 2|||Manual Length = 2386 Score = 25.0 bits (52), Expect = 10.0 Identities = 11/39 (28%), Positives = 20/39 (51%) Frame = +1 Query: 520 IICKISKAFRFNGNLICNLFSSIPCHFF*ISFSHVLTAV 636 II K+ + +C L IP +FF ++ S +++ V Sbjct: 1869 IISSRKKSLELMNSNVCRLSMKIPQYFFLVALSQMISRV 1907 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,510,726 Number of Sequences: 5004 Number of extensions: 48999 Number of successful extensions: 105 Number of sequences better than 10.0: 3 Number of HSP's better than 10.0 without gapping: 102 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 105 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 309878492 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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