Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmmt10j16
(737 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pomb... 104 1e-23
SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch... 102 5e-23
SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces... 90 3e-19
SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma... 65 1e-11
SPAC926.03 |rlc1||myosin II regulatory light chain |Schizosaccha... 62 8e-11
SPAC15A10.08 |ain1||alpha-actinin|Schizosaccharomyces pombe|chr ... 35 0.014
SPAC1687.14c |||EF hand family protein, unknown role|Schizosacch... 31 0.23
SPBP16F5.03c |||phosphatidylinositol kinase |Schizosaccharomyces... 29 0.69
SPAC1565.07c |||TATA binding protein interacting protein |Schizo... 27 2.1
SPAC20G8.06 |||CCR4-Not complex subunit Not1 |Schizosaccharomyce... 27 2.8
SPBC28F2.12 |rpb1||DNA-directed RNA polymerase II large subunit|... 26 4.9
SPAC23C11.15 |pst2||Clr6 histone deacetylase complex subunit Pst... 26 4.9
SPAC1093.06c |dhc1|SPAC30C2.01c|dynein heavy chain |Schizosaccha... 25 8.5
>SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 141
Score = 104 bits (250), Expect = 1e-23
Identities = 53/134 (39%), Positives = 82/134 (61%)
Frame = +1
Query: 148 FQEAFQLFDSRGDGKIHVAQIGDALRALGQNPTESDVKKCTLHLKPDERISFEVFLPIYQ 327
+++AF LFD G G+I IGD LRA GQNPT +++ + L P E + E FL +
Sbjct: 8 YKQAFSLFDRHGTGRIPKTSIGDLLRACGQNPTLAEITEIESTL-PAE-VDMEQFLQVLN 65
Query: 328 AISKARSGDTANDFIEGLRHFDKDGNGFISSAELRHLLSTLGEKLSDDEVEQLLQGQEDS 507
+ +F++G + FDKD G I ELR++L++LGEKLS++E+++LL+G
Sbjct: 66 RPNGFDMPGDPEEFVKGFQVFDKDATGMIGVGELRYVLTSLGEKLSNEEMDELLKGVPVK 125
Query: 508 QGNINYENFVHLIM 549
G +NY +FV +I+
Sbjct: 126 DGMVNYHDFVQMIL 139
Score = 27.5 bits (58), Expect = 2.1
Identities = 12/38 (31%), Positives = 21/38 (55%)
Frame = +1
Query: 145 EFQEAFQLFDSRGDGKIHVAQIGDALRALGQNPTESDV 258
EF + FQ+FD G I V ++ L +LG+ + ++
Sbjct: 78 EFVKGFQVFDKDATGMIGVGELRYVLTSLGEKLSNEEM 115
>SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 150
Score = 102 bits (245), Expect = 5e-23
Identities = 55/143 (38%), Positives = 85/143 (59%), Gaps = 3/143 (2%)
Frame = +1
Query: 127 SEDQLAEFQEAFQLFDSRGDGKIHVAQIGDALRALGQNPTESDVKKCTLHLKPDER--IS 300
+++Q+AEF+EAF LFD DG I ++G +R+LGQ+PT ++++ + D I
Sbjct: 7 TDEQIAEFREAFSLFDRDQDGNITSNELGVVMRSLGQSPTAAELQDMINEVDADGNGTID 66
Query: 301 FEVFLPIYQAISKARSGDTANDFIEGLRHFDKDGNGFISSAELRHLLSTLGEKLSDDEVE 480
F FL + K + D + E + FDKDGNG+I+ EL H+L++LGE+LS +EV
Sbjct: 67 FTEFLTMMAR--KMKDTDNEEEVREAFKVFDKDGNGYITVEELTHVLTSLGERLSQEEVA 124
Query: 481 QLL-QGQEDSQGNINYENFVHLI 546
++ + D G INYE F +I
Sbjct: 125 DMIREADTDGDGVINYEEFSRVI 147
>SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 143
Score = 90.2 bits (214), Expect = 3e-19
Identities = 50/142 (35%), Positives = 84/142 (59%), Gaps = 1/142 (0%)
Frame = +1
Query: 127 SEDQLAEFQEAFQLFDSRGDGKIHVAQIGDALRALGQNPTESDVKKCTLHLKPDERISFE 306
S++Q E +EAF L+D DG I + +G LR+LG N T++++ K + L + I +
Sbjct: 4 SKEQTDEMKEAFVLYDIDKDGLIPTSHVGSVLRSLGINVTDAELAKLSNEL--GDAIDEK 61
Query: 307 VFLPIYQAISKARSGDTANDFIEGLRHFDKDGNGFISSAELRHLLSTLGEKLSDDEVEQL 486
F+ +K R ++ ++I+ R FDKD +G+I +A+ + TLGEKLSD+EV+ +
Sbjct: 62 KFMSFVS--NKLRETESEEEYIKAFRVFDKDNSGYIETAKFADYMKTLGEKLSDNEVQLM 119
Query: 487 LQ-GQEDSQGNINYENFVHLIM 549
+Q + G+ +Y +FV IM
Sbjct: 120 VQEADPTNSGSFDYYDFVQRIM 141
Score = 35.5 bits (78), Expect = 0.008
Identities = 16/65 (24%), Positives = 35/65 (53%)
Frame = +1
Query: 145 EFQEAFQLFDSRGDGKIHVAQIGDALRALGQNPTESDVKKCTLHLKPDERISFEVFLPIY 324
E+ +AF++FD G I A+ D ++ LG+ ++++V+ P SF+ + +
Sbjct: 79 EYIKAFRVFDKDNSGYIETAKFADYMKTLGEKLSDNEVQLMVQEADPTNSGSFDYYDFVQ 138
Query: 325 QAISK 339
+ ++K
Sbjct: 139 RIMAK 143
>SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr
3|||Manual
Length = 176
Score = 64.9 bits (151), Expect = 1e-11
Identities = 43/144 (29%), Positives = 68/144 (47%), Gaps = 3/144 (2%)
Frame = +1
Query: 127 SEDQLAEFQEAFQLFDSRGDGKIHVAQIGDALRALGQNPTESDVKKCTLHLKPDER--IS 300
+E+Q + EAF+LFDS D I ++ A+RALG N +S+V K + +
Sbjct: 32 TEEQRQDINEAFKLFDSDKDNAIDYHELRAAMRALGFNAEKSEVLKILRDFDKTGKGYLQ 91
Query: 301 FEVFLPIYQAISKARSGDTANDFIEGLRHFDKDGNGFISSAELRHLLSTLGEKLSDDEVE 480
E F+ + K D + FD D G IS LR + L E + D E+E
Sbjct: 92 MEDFVRV--MTEKIVERDPLEEIKRAFELFDDDETGKISLRNLRRVAKELNENIDDQELE 149
Query: 481 QLLQGQE-DSQGNINYENFVHLIM 549
+++ + D G IN + F+ ++M
Sbjct: 150 AMIEEFDLDQDGEINEQEFIAIMM 173
>SPAC926.03 |rlc1||myosin II regulatory light chain
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 184
Score = 62.1 bits (144), Expect = 8e-11
Identities = 43/143 (30%), Positives = 66/143 (46%)
Frame = +1
Query: 118 AGYSEDQLAEFQEAFQLFDSRGDGKIHVAQIGDALRALGQNPTESDVKKCTLHLKPDERI 297
A + Q+ E +EAF L D GDG I + L +L Q+ +E + + P I
Sbjct: 40 AQLTSSQIQELKEAFALLDKDGDGNIGREDVKTMLTSLNQDASEDSINHMFESINPP--I 97
Query: 298 SFEVFLPIYQAISKARSGDTANDFIEGLRHFDKDGNGFISSAELRHLLSTLGEKLSDDEV 477
+ FL ++ S ND +E FD +G I + +R LS++G+++ EV
Sbjct: 98 NLAAFLTAMGSMLCRIS--PRNDLLEAFSTFDDTQSGKIPISTMRDALSSMGDRMDPQEV 155
Query: 478 EQLLQGQEDSQGNINYENFVHLI 546
E +L+ S G YE FV I
Sbjct: 156 ESILRSY-TSHGVFYYEKFVDAI 177
>SPAC15A10.08 |ain1||alpha-actinin|Schizosaccharomyces pombe|chr
1|||Manual
Length = 621
Score = 34.7 bits (76), Expect = 0.014
Identities = 15/57 (26%), Positives = 28/57 (49%)
Frame = +1
Query: 382 RHFDKDGNGFISSAELRHLLSTLGEKLSDDEVEQLLQGQEDSQGNINYENFVHLIMQ 552
RHF+K + ++ E L++LG +E L +S+ + YE F ++M+
Sbjct: 494 RHFEKKKSNMLNEVEFYAALASLGLVYDTEEGTALFHRAANSEEGVTYERFTEIVME 550
>SPAC1687.14c |||EF hand family protein, unknown
role|Schizosaccharomyces pombe|chr 1|||Manual
Length = 76
Score = 30.7 bits (66), Expect = 0.23
Identities = 16/52 (30%), Positives = 27/52 (51%)
Frame = +1
Query: 388 FDKDGNGFISSAELRHLLSTLGEKLSDDEVEQLLQGQEDSQGNINYENFVHL 543
FD G+I +LR + LGE L+ +++ QL+ + G ++ E F L
Sbjct: 21 FDVTHKGYIDFEDLRRSCAQLGENLTKEQL-QLMLDLAGTNGKVSREEFAEL 71
Score = 27.5 bits (58), Expect = 2.1
Identities = 18/64 (28%), Positives = 30/64 (46%)
Frame = +1
Query: 145 EFQEAFQLFDSRGDGKIHVAQIGDALRALGQNPTESDVKKCTLHLKPDERISFEVFLPIY 324
E +EAF LFD G I + + LG+N T+ ++ + ++S E F ++
Sbjct: 13 EAEEAFDLFDVTHKGYIDFEDLRRSCAQLGENLTKEQLQLMLDLAGTNGKVSREEFAELW 72
Query: 325 QAIS 336
IS
Sbjct: 73 IHIS 76
>SPBP16F5.03c |||phosphatidylinositol kinase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 3699
Score = 29.1 bits (62), Expect = 0.69
Identities = 19/57 (33%), Positives = 29/57 (50%)
Frame = -3
Query: 480 LYLIITELLSESREQVSQFRRRDEPIAIFVKMAQTLNKVISSVATACFRYGLVNWQK 310
L+L+ L++ +Q + R + IAIF K + +K I SVA R+ L QK
Sbjct: 1377 LHLLFASLVAHKFDQPQHAQTRTKIIAIFFKDLYSPHKEIYSVAIDALRHVLSQNQK 1433
>SPAC1565.07c |||TATA binding protein interacting protein
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1220
Score = 27.5 bits (58), Expect = 2.1
Identities = 20/66 (30%), Positives = 37/66 (56%), Gaps = 4/66 (6%)
Frame = +1
Query: 304 EVFLPIYQAISKARSGDTANDFI--EGLRHFDKDGNGFISS-AELRHLLSTLGEK-LSDD 471
++F+ + I+K +G + I + RH DK GN F ++ EL +L +G+K L++
Sbjct: 643 DIFMSVTD-ITKIENGTKIYEEILQDCCRHIDKSGNEFTTAYLELLEVLLKVGQKYLAES 701
Query: 472 EVEQLL 489
+E +L
Sbjct: 702 LLEHIL 707
>SPAC20G8.06 |||CCR4-Not complex subunit Not1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 2100
Score = 27.1 bits (57), Expect = 2.8
Identities = 16/48 (33%), Positives = 27/48 (56%)
Frame = +1
Query: 412 ISSAELRHLLSTLGEKLSDDEVEQLLQGQEDSQGNINYENFVHLIMQG 555
I A++ LLSTL E D ++EQ+ Q + + Y +F+ ++QG
Sbjct: 42 IVKAQILFLLSTLREDQYDTKLEQIRQLINKNAPRV-YHHFLRRLIQG 88
>SPBC28F2.12 |rpb1||DNA-directed RNA polymerase II large
subunit|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1752
Score = 26.2 bits (55), Expect = 4.9
Identities = 16/64 (25%), Positives = 29/64 (45%), Gaps = 2/64 (3%)
Frame = -2
Query: 226 LLTHLRSERHVSFHLHEN--RRAGRLLEILPIDPLNIQPYYESTNEIEGNEIGIFKGCDL 53
+ TH+ SE L+E R ++ +LP+ P +++P G + K D+
Sbjct: 216 IFTHISSEDLAHLGLNEQYARPDWMIITVLPVPPPSVRPSISVDGTSRGEDDLTHKLSDI 275
Query: 52 SKSN 41
K+N
Sbjct: 276 IKAN 279
>SPAC23C11.15 |pst2||Clr6 histone deacetylase complex subunit
Pst2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1075
Score = 26.2 bits (55), Expect = 4.9
Identities = 18/49 (36%), Positives = 26/49 (53%)
Frame = -3
Query: 552 LHDEVNKVLIVDISLRVFLSLQELLYLIITELLSESREQVSQFRRRDEP 406
+HD+V+K S+ VF L LLY + E L +QVS ++R P
Sbjct: 735 MHDDVDKEQFGYSSMYVFFRLFNLLYERLYE-LQRLEDQVSIIQQRIIP 782
>SPAC1093.06c |dhc1|SPAC30C2.01c|dynein heavy chain
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 4196
Score = 25.4 bits (53), Expect = 8.5
Identities = 16/67 (23%), Positives = 31/67 (46%)
Frame = -3
Query: 648 LTVSHYGSNCMVTDLVSLQIKVLQQMMT*NSALHDEVNKVLIVDISLRVFLSLQELLYLI 469
L S+Y ++ D V Q+K L Q +S + + + + RV S+ + L+L+
Sbjct: 539 LETSYYNCMTVLEDEVIAQLKSLLQYSKTSSQMFTTLMRFQPLFFRTRVRTSISDCLHLL 598
Query: 468 ITELLSE 448
+ + E
Sbjct: 599 VNRIKQE 605
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,911,396
Number of Sequences: 5004
Number of extensions: 58684
Number of successful extensions: 208
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 190
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 196
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 349251756
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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