SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= bmmt10j11
         (646 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC18B11.04 |ncs1||related to neuronal calcium sensor Ncs1|Schi...    29   0.76 
SPAC2E12.03c |||G-protein coupled receptor |Schizosaccharomyces ...    28   1.0  
SPAC16C9.07 |ppk5|SPAC2G11.01, mug189|serine/threonine protein k...    28   1.3  
SPBC428.01c |nup107|SPBC582.11c|nucleoporin Nup107|Schizosacchar...    27   2.3  
SPAC2E1P5.03 |||DNAJ domain protein Erj5|Schizosaccharomyces pom...    27   2.3  
SPBC530.14c |dsk1||SR protein-specific kinase Dsk1|Schizosacchar...    27   3.1  
SPBC354.05c |sre2||membrane-tethered transcription factor |Schiz...    26   4.0  
SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch...    26   4.0  
SPAC6G10.05c |||TRAPP complex subunit Trs120 |Schizosaccharomyce...    26   4.0  
SPBC31F10.14c |hip3|hir3|HIRA interacting protein Hip3|Schizosac...    26   4.0  
SPCC4G3.12c |||ubiquitin-protein ligase E3 |Schizosaccharomyces ...    26   4.0  
SPAC6B12.12 |tom70||mitochondrial TOM complex subunit Tom70|Schi...    26   4.0  
SPAC18G6.05c |||translation elongation regulator Gcn1 |Schizosac...    25   7.1  
SPAP27G11.11c |||dubious|Schizosaccharomyces pombe|chr 1|||Manual      25   7.1  
SPAC4F10.09c |||ribosome biogenesis protein Noc1 |Schizosaccharo...    25   7.1  
SPCC285.10c |||SPRY domain protein|Schizosaccharomyces pombe|chr...    25   7.1  
SPBC651.07 |csa1||sequence orphan|Schizosaccharomyces pombe|chr ...    25   7.1  
SPCC1620.01c |lsm2||U6 snRNP-associated protein core protein|Sch...    25   7.1  
SPCC1840.08c |||protein disulfide isomerase |Schizosaccharomyces...    25   9.3  
SPAC1F3.06c |spo15||sporulation protein Spo15|Schizosaccharomyce...    25   9.3  
SPBC17A3.05c |||DNAJ/DUF1977 DNAJB12 homolog|Schizosaccharomyces...    25   9.3  

>SPAC18B11.04 |ncs1||related to neuronal calcium sensor
           Ncs1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 190

 Score = 28.7 bits (61), Expect = 0.76
 Identities = 13/47 (27%), Positives = 28/47 (59%)
 Frame = +1

Query: 244 GQVSKEEWISLWEKFSSSPSDWQNLYCKFIFQLEDASNDGSIDSEEF 384
           G ++K E+  ++++F   P    + + +++F + DA  +G ID +EF
Sbjct: 41  GHLNKSEFQKIYKQFF--PFGDPSAFAEYVFNVFDADKNGYIDFKEF 85



 Score = 27.5 bits (58), Expect = 1.8
 Identities = 15/66 (22%), Positives = 32/66 (48%), Gaps = 2/66 (3%)
 Frame = +1

Query: 355 NDGSIDSEEFSSVYASFGLDKAEAASAFQKLSKGKSS--VSFAEFQELFKEYFASEDVNA 528
           +   +  ++   +  S   DK E    ++   K   S  ++ +EFQ+++K++F   D +A
Sbjct: 4   SQSKLSQDQLQDLVRSTRFDKKELQQWYKGFFKDCPSGHLNKSEFQKIYKQFFPFGDPSA 63

Query: 529 PGNFVF 546
              +VF
Sbjct: 64  FAEYVF 69



 Score = 26.6 bits (56), Expect = 3.1
 Identities = 33/118 (27%), Positives = 51/118 (43%), Gaps = 9/118 (7%)
 Frame = +1

Query: 58  HVFTAFFDTNGSGTIDKKDFELAIERISKSRGWSAGDAQYKEVQDTLLKVWDGLSSADTD 237
           +VF  F D + +G ID K+F  A+     SRG         E+ D L  +W      D D
Sbjct: 67  YVFNVF-DADKNGYIDFKEFICALS--VTSRG---------ELNDKL--IW-AFQLYDLD 111

Query: 238 NDGQVSKEEWI----SLWEKFSS-----SPSDWQNLYCKFIFQLEDASNDGSIDSEEF 384
           N+G +S +E +    ++++   S        D        IF + D + DG +  EEF
Sbjct: 112 NNGLISYDEMLRIVDAIYKMVGSMVKLPEDEDTPEKRVNKIFNMMDKNKDGQLTLEEF 169


>SPAC2E12.03c |||G-protein coupled receptor |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 283

 Score = 28.3 bits (60), Expect = 1.0
 Identities = 12/33 (36%), Positives = 19/33 (57%), Gaps = 3/33 (9%)
 Frame = +1

Query: 232 TDNDGQVSKEEWISLWE---KFSSSPSDWQNLY 321
           T+ DG    EE+  ++    KF++ P+ WQN Y
Sbjct: 240 TEEDGDEKDEEYSDIFSIKGKFNTRPNAWQNAY 272


>SPAC16C9.07 |ppk5|SPAC2G11.01, mug189|serine/threonine protein
           kinase Ppk5 |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 836

 Score = 27.9 bits (59), Expect = 1.3
 Identities = 12/30 (40%), Positives = 17/30 (56%)
 Frame = -2

Query: 93  AAIGVEESGENVQQLLLSKI*HHFADLSRL 4
           +A+G+EE     + L   K  HHFADL  +
Sbjct: 85  SAVGIEEKSFKDEHLAQKKGLHHFADLKEI 114


>SPBC428.01c |nup107|SPBC582.11c|nucleoporin
           Nup107|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 794

 Score = 27.1 bits (57), Expect = 2.3
 Identities = 16/44 (36%), Positives = 22/44 (50%)
 Frame = +1

Query: 259 EEWISLWEKFSSSPSDWQNLYCKFIFQLEDASNDGSIDSEEFSS 390
           EEWI LW+  S +   W  +   + F+L +    G I S  FSS
Sbjct: 100 EEWIELWDLESRT---WDLIQRLYSFRLSE--QQGHIQSHAFSS 138


>SPAC2E1P5.03 |||DNAJ domain protein Erj5|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 303

 Score = 27.1 bits (57), Expect = 2.3
 Identities = 16/29 (55%), Positives = 18/29 (62%), Gaps = 1/29 (3%)
 Frame = +1

Query: 220 SSADTDNDGQVSKEEWISLWEKFS-SSPS 303
           SS DTD+DGQ+  EE      K S SSPS
Sbjct: 245 SSEDTDSDGQMEDEEKSDSVHKSSFSSPS 273


>SPBC530.14c |dsk1||SR protein-specific kinase
           Dsk1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 544

 Score = 26.6 bits (56), Expect = 3.1
 Identities = 10/26 (38%), Positives = 17/26 (65%)
 Frame = +2

Query: 239 TTAKSPKKSGFPSGRSFPAAPVTGRI 316
           T AK+P K+  P+G+  P++P T  +
Sbjct: 278 TFAKNPTKNSKPAGQVIPSSPFTSTL 303


>SPBC354.05c |sre2||membrane-tethered transcription factor
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 793

 Score = 26.2 bits (55), Expect = 4.0
 Identities = 20/58 (34%), Positives = 24/58 (41%), Gaps = 3/58 (5%)
 Frame = +2

Query: 233 PITTAKSPKKSGFPSGRSFPAA---PVTGRIYTASLYFNLRMLVMTGPSTARNSLRST 397
           PI+   S    G P   S  +A    V      AS  FNL   V   P  ARNS+ S+
Sbjct: 57  PISHVPSVGTRGIPRSYSMASAYPYQVNSNSMDASTAFNLNQDVADKPMLARNSIDSS 114


>SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 150

 Score = 26.2 bits (55), Expect = 4.0
 Identities = 31/108 (28%), Positives = 44/108 (40%)
 Frame = +1

Query: 79  DTNGSGTIDKKDFELAIERISKSRGWSAGDAQYKEVQDTLLKVWDGLSSADTDNDGQVSK 258
           D +G+GTID  +F   + R  K       D   +E      KV+D       D +G ++ 
Sbjct: 58  DADGNGTIDFTEFLTMMARKMK-------DTDNEEEVREAFKVFD------KDGNGYITV 104

Query: 259 EEWISLWEKFSSSPSDWQNLYCKFIFQLEDASNDGSIDSEEFSSVYAS 402
           EE   +        S  Q      I +  D   DG I+ EEFS V +S
Sbjct: 105 EELTHVLTSLGERLS--QEEVADMIREA-DTDGDGVINYEEFSRVISS 149


>SPAC6G10.05c |||TRAPP complex subunit Trs120 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 1210

 Score = 26.2 bits (55), Expect = 4.0
 Identities = 10/35 (28%), Positives = 21/35 (60%)
 Frame = +2

Query: 59  TFSPLSSTPMAAEPLIKRTLNLPLKGYPNREAGVL 163
           TF+  SS P +  P  +R +++ ++  PN + G++
Sbjct: 876 TFTVESSQPFSLSPGERREIHIKIRAKPNSQEGLI 910


>SPBC31F10.14c |hip3|hir3|HIRA interacting protein
           Hip3|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1630

 Score = 26.2 bits (55), Expect = 4.0
 Identities = 11/34 (32%), Positives = 18/34 (52%)
 Frame = -3

Query: 158 LQPLDLDILSMASSKSFLSMVPLPLVSKKAVKTC 57
           L  LDL I S+ +  SF   +P P   ++++  C
Sbjct: 201 LPQLDLSISSLYTLPSFFPQLPTPSFHRRSINLC 234


>SPCC4G3.12c |||ubiquitin-protein ligase E3 |Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 821

 Score = 26.2 bits (55), Expect = 4.0
 Identities = 9/27 (33%), Positives = 14/27 (51%)
 Frame = +1

Query: 235 DNDGQVSKEEWISLWEKFSSSPSDWQN 315
           DND Q  +  W S+   F+  P ++ N
Sbjct: 253 DNDSQTRRSSWSSIASAFNDFPEEFPN 279


>SPAC6B12.12 |tom70||mitochondrial TOM complex subunit
           Tom70|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 625

 Score = 26.2 bits (55), Expect = 4.0
 Identities = 15/48 (31%), Positives = 25/48 (52%)
 Frame = +1

Query: 418 AEAASAFQKLSKGKSSVSFAEFQELFKEYFASEDVNAPGNFVFGKTSF 561
           A  A A++ L KG+  +S+ + +E     F+S  VNA  + + G   F
Sbjct: 311 AALAEAYEYLEKGEYQLSYDKAKESCLGSFSSPSVNARTHNLVGTFKF 358


>SPAC18G6.05c |||translation elongation regulator Gcn1
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 2670

 Score = 25.4 bits (53), Expect = 7.1
 Identities = 14/45 (31%), Positives = 24/45 (53%)
 Frame = -3

Query: 326 LQYKFCQSLGLLENFSQREIHSSLETWPSLSVSALLNPSQTFKSV 192
           L ++FC SLGLL +  Q +++      PS+  + L +P   F  +
Sbjct: 257 LYHEFCYSLGLLLSVDQLKLY----LLPSIEKALLRSPEIIFSGI 297


>SPAP27G11.11c |||dubious|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 73

 Score = 25.4 bits (53), Expect = 7.1
 Identities = 18/40 (45%), Positives = 21/40 (52%), Gaps = 2/40 (5%)
 Frame = -3

Query: 314 FCQSLGLLENFSQREIHSS--LETWPSLSVSALLNPSQTF 201
           +C S+  LE  S R   SS  L    SLS+    NPSQTF
Sbjct: 26  YCVSIYRLEECSGRFKPSSYSLHLLKSLSIIICENPSQTF 65


>SPAC4F10.09c |||ribosome biogenesis protein Noc1
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 860

 Score = 25.4 bits (53), Expect = 7.1
 Identities = 16/61 (26%), Positives = 20/61 (32%)
 Frame = +1

Query: 337 QLEDASNDGSIDSEEFSSVYASFGLDKAEAASAFQKLSKGKSSVSFAEFQELFKEYFASE 516
           QLE    +   DSEE        G D  ++A      S           +E   E   SE
Sbjct: 736 QLEMDEEESDFDSEEMDKAMTDMGSDSEQSADENDNESMASEEKPMFSDEENLSEIAHSE 795

Query: 517 D 519
           D
Sbjct: 796 D 796


>SPCC285.10c |||SPRY domain protein|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 382

 Score = 25.4 bits (53), Expect = 7.1
 Identities = 19/58 (32%), Positives = 24/58 (41%)
 Frame = -2

Query: 252 DLAVVIGVSAAQPIPDLQKRVLNFFVLSISSTPASRFGYPFNGKFKVLFINGSAAIGV 79
           D  V IG+ A +P P  +    NF+  +  S    R   PF GK    F      IGV
Sbjct: 179 DTLVSIGL-ATKPYPPFRLPGWNFWSTAYVSDGTRRSNSPFTGKPYSSFYQQGDVIGV 235


>SPBC651.07 |csa1||sequence orphan|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 268

 Score = 25.4 bits (53), Expect = 7.1
 Identities = 14/48 (29%), Positives = 25/48 (52%)
 Frame = +1

Query: 229 DTDNDGQVSKEEWISLWEKFSSSPSDWQNLYCKFIFQLEDASNDGSID 372
           D +N  Q  KEEW +   + + +PS + +     + QL+D S   +I+
Sbjct: 137 DFNNKDQFWKEEWSTEQYEIAENPSGYASA-LHTLTQLDDGSETNAIN 183


>SPCC1620.01c |lsm2||U6 snRNP-associated protein core
           protein|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 96

 Score = 25.4 bits (53), Expect = 7.1
 Identities = 19/50 (38%), Positives = 24/50 (48%)
 Frame = -2

Query: 189 LNFFVLSISSTPASRFGYPFNGKFKVLFINGSAAIGVEESGENVQQLLLS 40
           LN  + +IS   AS+  YP     K LFI GS    V  S   V  +LL+
Sbjct: 36  LNVKLENISVVDASK--YPHMAAVKDLFIRGSVVRYVHMSSAYVDTILLA 83


>SPCC1840.08c |||protein disulfide isomerase |Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 561

 Score = 25.0 bits (52), Expect = 9.3
 Identities = 11/32 (34%), Positives = 20/32 (62%)
 Frame = +1

Query: 25  MVSDFRKKKLLHVFTAFFDTNGSGTIDKKDFE 120
           +VS F+++KLL+  +A    N  G+ +  DF+
Sbjct: 75  LVSSFKERKLLNTNSANLRLNSIGSYEMIDFK 106


>SPAC1F3.06c |spo15||sporulation protein Spo15|Schizosaccharomyces
            pombe|chr 1|||Manual
          Length = 1957

 Score = 25.0 bits (52), Expect = 9.3
 Identities = 21/66 (31%), Positives = 31/66 (46%)
 Frame = +1

Query: 250  VSKEEWISLWEKFSSSPSDWQNLYCKFIFQLEDASNDGSIDSEEFSSVYASFGLDKAEAA 429
            +S EE +S   +  SS  D +N   +    L+D S   S   E+  S+ +S      E A
Sbjct: 1421 ISLEESLSNQRQKESSLLDAKN---ELEHMLDDTSRKNSSLMEKIESINSSLDDKSFELA 1477

Query: 430  SAFQKL 447
            SA +KL
Sbjct: 1478 SAVEKL 1483


>SPBC17A3.05c |||DNAJ/DUF1977 DNAJB12 homolog|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 403

 Score = 25.0 bits (52), Expect = 9.3
 Identities = 8/22 (36%), Positives = 13/22 (59%)
 Frame = +1

Query: 484 QELFKEYFASEDVNAPGNFVFG 549
           ++LF  +F  +  + PG F FG
Sbjct: 212 EDLFNSFFGDQFFSGPGTFFFG 233


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,787,353
Number of Sequences: 5004
Number of extensions: 61428
Number of successful extensions: 242
Number of sequences better than 10.0: 21
Number of HSP's better than 10.0 without gapping: 225
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 242
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 289756512
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -