BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= bmmt10j02 (689 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC16E8.17c |||succinate-CoA ligase alpha subunit|Schizosacchar... 248 6e-67 SPBP4H10.19c |||calreticulin/calnexin homolog|Schizosaccharomyce... 28 1.5 SPBC28F2.12 |rpb1||DNA-directed RNA polymerase II large subunit|... 27 1.9 SPAC3G6.09c |tps2||trehalose-phosphate synthase Tps2 |Schizosacc... 27 3.4 SPCC1235.05c |fft2||fun thirty related protein Fft2|Schizosaccha... 26 4.5 SPAC4F8.15 |itr1|SPAC7D4.01|myo-inositol transporter Itr1|Schizo... 26 5.9 SPBC16C6.02c |vps1302|vps13b|chorein homolog|Schizosaccharomyces... 25 7.8 SPBC582.06c |mcp6|hrs1, mug3|meiosis specific coiled-coil protei... 25 7.8 SPAC31A2.05c |mis4||cohesin loading factor Mis4|Schizosaccharomy... 25 7.8 >SPAC16E8.17c |||succinate-CoA ligase alpha subunit|Schizosaccharomyces pombe|chr 1|||Manual Length = 331 Score = 248 bits (607), Expect = 6e-67 Identities = 120/194 (61%), Positives = 143/194 (73%) Frame = +1 Query: 106 LSKFKDGLKLGNVRFASGNPYAETRKNLILTSETKVIVQGFTGKQGTFHSQQALDYGTKV 285 L +F +L N S + Y +T NL++ S+TKVI QGFTGKQGTFH+Q A+DYGTKV Sbjct: 12 LRRFSSSSQLKN----SKSLYEQTIPNLMINSDTKVIFQGFTGKQGTFHAQHAMDYGTKV 67 Query: 286 VGGVSPKKAGTEHLGKPVFGTVKEAKAGTGATASVIYVPPPGXXXXXXXXXXXXMPLIVC 465 VGG +PKKAGT HLGKPVFGT++EA T A AS ++VPPP +PLIV Sbjct: 68 VGGTNPKKAGTTHLGKPVFGTIEEAMKETKADASAVFVPPPLAAGAIEEAIAAEVPLIVA 127 Query: 466 ITEGVPQHDMVRVKHALLRQNKSRLVGPNCPGIIAPEKCKIGIMPAAVHKRGCIGVVSRS 645 ITEG+PQHDM+RV L Q+KSRLVGPNCPGII P +CKIGIMP+ +HK GCIG+VSRS Sbjct: 128 ITEGIPQHDMLRVSDILKTQSKSRLVGPNCPGIIRPGQCKIGIMPSHIHKPGCIGIVSRS 187 Query: 646 GTLTYEACHQTTIT 687 GTLTYEA +QTT T Sbjct: 188 GTLTYEAVNQTTQT 201 >SPBP4H10.19c |||calreticulin/calnexin homolog|Schizosaccharomyces pombe|chr 2|||Manual Length = 381 Score = 27.9 bits (59), Expect = 1.5 Identities = 16/50 (32%), Positives = 22/50 (44%) Frame = -3 Query: 585 FTLLGSNDTRTVRTNQSRLVLSQEGMFYSHHIMLWHTFCNAHNEGHFSLN 436 +TLL N T +R +QS + G + H W T N N + S N Sbjct: 186 YTLLLQNTTVVIRRDQSVVYTGDVGTNFFHSPTKWITHSNVSNGYYTSRN 235 >SPBC28F2.12 |rpb1||DNA-directed RNA polymerase II large subunit|Schizosaccharomyces pombe|chr 2|||Manual Length = 1752 Score = 27.5 bits (58), Expect = 1.9 Identities = 20/70 (28%), Positives = 32/70 (45%), Gaps = 1/70 (1%) Frame = +1 Query: 163 PYAETRKNLILTSETKVIVQGFTGKQGTFHSQQALDYGTKVVG-GVSPKKAGTEHLGKPV 339 PY+ R NL +TS F G + H Q+ + ++ + PK+ + KPV Sbjct: 470 PYSTFRLNLSVTSPYNA---DFDGDEMNMHVPQSEETRAEIQEITMVPKQIVSPQSNKPV 526 Query: 340 FGTVKEAKAG 369 G V++ AG Sbjct: 527 MGIVQDTLAG 536 >SPAC3G6.09c |tps2||trehalose-phosphate synthase Tps2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 849 Score = 26.6 bits (56), Expect = 3.4 Identities = 14/44 (31%), Positives = 24/44 (54%) Frame = -3 Query: 321 FCTSLLW*HSSNNFSTIVKGLLAVEGTLLSSETLNNHFCFTCKD 190 FCTS ++ S + I+KG+LA + +++ HF TC + Sbjct: 252 FCTSEVFRCLSKR-AEILKGVLASNVIAMQTDSYTRHFLSTCSN 294 >SPCC1235.05c |fft2||fun thirty related protein Fft2|Schizosaccharomyces pombe|chr 3|||Manual Length = 1284 Score = 26.2 bits (55), Expect = 4.5 Identities = 10/34 (29%), Positives = 20/34 (58%) Frame = -1 Query: 374 PVPAFASLTVPNTGLPRCSVPAFFGDTPPTTLVP 273 P+P++ + ++PN G+P ++P P T +P Sbjct: 124 PLPSYNTASLPNAGIP-AAMPGMPSGYPGTVPIP 156 >SPAC4F8.15 |itr1|SPAC7D4.01|myo-inositol transporter Itr1|Schizosaccharomyces pombe|chr 1|||Manual Length = 575 Score = 25.8 bits (54), Expect = 5.9 Identities = 12/41 (29%), Positives = 19/41 (46%), Gaps = 1/41 (2%) Frame = +1 Query: 52 PFRRFTFNNSTMAMPVRVLSKFKDGLK-LGNVRFASGNPYA 171 PF F N T +PV +S+ +G + N + P+A Sbjct: 33 PFESSVFENKTQVLPVDSVSRLSNGARSRSNSNISLSEPHA 73 >SPBC16C6.02c |vps1302|vps13b|chorein homolog|Schizosaccharomyces pombe|chr 2|||Manual Length = 3131 Score = 25.4 bits (53), Expect = 7.8 Identities = 13/69 (18%), Positives = 32/69 (46%) Frame = +1 Query: 64 FTFNNSTMAMPVRVLSKFKDGLKLGNVRFASGNPYAETRKNLILTSETKVIVQGFTGKQG 243 + NN+ V+V F+ +KLG V ++T++N +++ ++ F + Sbjct: 801 YKLNNTPSEQSVKVSHFFEFNMKLGEVTLILCREDSQTKRNSMVSVNFAKLLLSFNQFED 860 Query: 244 TFHSQQALD 270 H + +++ Sbjct: 861 NSHLRMSIN 869 >SPBC582.06c |mcp6|hrs1, mug3|meiosis specific coiled-coil protein Mcp6|Schizosaccharomyces pombe|chr 2|||Manual Length = 327 Score = 25.4 bits (53), Expect = 7.8 Identities = 13/30 (43%), Positives = 18/30 (60%) Frame = -1 Query: 149 NRTLPSFSPSLNLERTRTGIAIVELLNVNL 60 NR PS+S SLN T ++ VE +N +L Sbjct: 297 NRAPPSYSFSLNCSHTYQPVSCVEPVNHDL 326 >SPAC31A2.05c |mis4||cohesin loading factor Mis4|Schizosaccharomyces pombe|chr 1|||Manual Length = 1583 Score = 25.4 bits (53), Expect = 7.8 Identities = 18/65 (27%), Positives = 29/65 (44%), Gaps = 3/65 (4%) Frame = -3 Query: 369 ACLCLFDCTKHRLTKMFCTSLLW*HS---SNNFSTIVKGLLAVEGTLLSSETLNNHFCFT 199 A L L +++ +F SL H+ NF+ I+ + ++ S+ NHF F Sbjct: 632 AALDLISLIVNKVLALFDLSLFEKHNIPAPTNFNDIISFIPSITRLNELSQVSFNHFYFL 691 Query: 198 CKDQI 184 CK I Sbjct: 692 CKGDI 696 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,906,822 Number of Sequences: 5004 Number of extensions: 60892 Number of successful extensions: 167 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 155 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 166 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 319939482 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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