Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmmt10i23
(712 letters)
Database: nematostella
59,808 sequences; 16,821,457 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SB_36368| Best HMM Match : 14-3-3 (HMM E-Value=0) 214 7e-56
SB_34219| Best HMM Match : No HMM Matches (HMM E-Value=.) 190 1e-48
SB_37955| Best HMM Match : 14-3-3 (HMM E-Value=6.5861e-44) 160 9e-40
SB_34217| Best HMM Match : No HMM Matches (HMM E-Value=.) 155 3e-38
SB_34218| Best HMM Match : No HMM Matches (HMM E-Value=.) 73 3e-13
SB_56522| Best HMM Match : Pox_A_type_inc (HMM E-Value=1.6e-30) 36 0.025
SB_20371| Best HMM Match : U-box (HMM E-Value=0.075) 33 0.17
SB_51222| Best HMM Match : No HMM Matches (HMM E-Value=.) 33 0.30
SB_22570| Best HMM Match : Filament (HMM E-Value=0.1) 31 0.70
SB_12729| Best HMM Match : Pox_A_type_inc (HMM E-Value=5.3e-08) 31 1.2
SB_7846| Best HMM Match : CoCoA (HMM E-Value=0.00016) 31 1.2
SB_43756| Best HMM Match : SlyX (HMM E-Value=3.7) 30 2.1
SB_36640| Best HMM Match : No HMM Matches (HMM E-Value=.) 29 2.8
SB_37483| Best HMM Match : Drf_FH1 (HMM E-Value=6.6) 29 4.9
SB_24031| Best HMM Match : No HMM Matches (HMM E-Value=.) 29 4.9
SB_2776| Best HMM Match : No HMM Matches (HMM E-Value=.) 28 6.5
SB_29939| Best HMM Match : No HMM Matches (HMM E-Value=.) 28 6.5
SB_11774| Best HMM Match : M (HMM E-Value=8.5e-09) 28 6.5
SB_47663| Best HMM Match : No HMM Matches (HMM E-Value=.) 28 8.6
SB_44921| Best HMM Match : Pepsin-I3 (HMM E-Value=6.4) 28 8.6
SB_39167| Best HMM Match : No HMM Matches (HMM E-Value=.) 28 8.6
SB_34997| Best HMM Match : No HMM Matches (HMM E-Value=.) 28 8.6
>SB_36368| Best HMM Match : 14-3-3 (HMM E-Value=0)
Length = 248
Score = 214 bits (522), Expect = 7e-56
Identities = 113/202 (55%), Positives = 138/202 (68%), Gaps = 3/202 (1%)
Frame = +1
Query: 115 REDNVYKAKLAEQAERYDEMVEAMKNVASRNVSDNELTVEERNLLSVAYKNVIGARRASW 294
RE+ ++ AK+AEQAERYD+MV AM V N+ EERNLLSVAYKNV+GARR+SW
Sbjct: 11 REELIHLAKMAEQAERYDDMVNAMSAVTKEGKPLND---EERNLLSVAYKNVVGARRSSW 67
Query: 295 RIISSIEQKEETKGAEDKLNMIRAYRSQVEKELRDICSDILGVLDKYLIPSSQTG---ES 465
R+ISS+EQK E+ + + YR + EL C+++L +L+ YL+ Q E+
Sbjct: 68 RVISSMEQK----APEEMAALTKKYREDITNELNGKCAEVLDILENYLLKDGQDDINTEA 123
Query: 466 KVFYYKMKGDYHRYLAEFATGNDRKEAAENSLVAYKAASDIAMTELPPTHPIRLGLALNF 645
KVFY KM+GDYHRYL E A G+ RKE E S AYK AS A EL P+HPIRLGLALNF
Sbjct: 124 KVFYLKMRGDYHRYLVEVAEGDSRKENIEKSREAYKDASAKA-EELSPSHPIRLGLALNF 182
Query: 646 SVFYYEILNSPDRACRLAKAAF 711
SVFYYEI N P AC+LAK AF
Sbjct: 183 SVFYYEIENKPPEACKLAKEAF 204
>SB_34219| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 309
Score = 190 bits (462), Expect = 1e-48
Identities = 99/182 (54%), Positives = 126/182 (69%), Gaps = 2/182 (1%)
Frame = +1
Query: 172 MVEAMKNVASRNVSDNELTVEERNLLSVAYKNVIGARRASWRIISSIEQKEETKGAEDKL 351
M +AMK +S+ L EERNLLSVAYKNV+GA+R+SWR+ISSIEQK E G+E K
Sbjct: 1 MAKAMKEATE--ISET-LEQEERNLLSVAYKNVVGAKRSSWRVISSIEQKLE--GSERKK 55
Query: 352 NMIRAYRSQVEKELRDICSDILGVLDKYLIPSSQTGESKVFYYKMKGDYHRYLAEFATGN 531
YR +E EL ++C +L +L+ LIP++Q+ ESKVFY KMKGDY+RY E A +
Sbjct: 56 QNTETYRQTIENELNEVCETVLKLLESKLIPNAQSTESKVFYLKMKGDYYRYEGEVAGAD 115
Query: 532 DRKEAAENSLVAYKAASDIAMTE--LPPTHPIRLGLALNFSVFYYEILNSPDRACRLAKA 705
R+E + ++ AY A +IA + LPPT PIRLGLALNFSVFYYEI+ +AC LAK
Sbjct: 116 RRREVVQKAMKAYSEAQEIAEKDPKLPPTDPIRLGLALNFSVFYYEIVEDSKQACDLAKK 175
Query: 706 AF 711
AF
Sbjct: 176 AF 177
Score = 67.3 bits (157), Expect = 1e-11
Identities = 35/63 (55%), Positives = 42/63 (66%), Gaps = 3/63 (4%)
Frame = +1
Query: 532 DRKEAAENSLVAYKAASDIAMT---ELPPTHPIRLGLALNFSVFYYEILNSPDRACRLAK 702
D E +L AYK A + A T +L PT PIRLGLALNFSVF+YEI + + AC+LAK
Sbjct: 204 DNLTVVEKALQAYKEAKEAAETGDGKLAPTDPIRLGLALNFSVFHYEIQENQEEACKLAK 263
Query: 703 AAF 711
AF
Sbjct: 264 QAF 266
>SB_37955| Best HMM Match : 14-3-3 (HMM E-Value=6.5861e-44)
Length = 251
Score = 160 bits (389), Expect = 9e-40
Identities = 82/128 (64%), Positives = 102/128 (79%), Gaps = 1/128 (0%)
Frame = +1
Query: 106 MSEREDNVYKAKLAEQAERYDEMVEAMKNVASRNVSDNELTVEERNLLSVAYKNVIGARR 285
M+++E++VY AKLAEQAERYD+MV +MK VA EL+ E+RNLLSVAYKNVIGARR
Sbjct: 1 MADKEEHVYMAKLAEQAERYDDMVNSMKEVAKMGT---ELSTEDRNLLSVAYKNVIGARR 57
Query: 286 ASWRIISSIEQKEETKGAE-DKLNMIRAYRSQVEKELRDICSDILGVLDKYLIPSSQTGE 462
ASWRII+SIEQKEE+KG + KL MIR YR +E+EL+ IC +IL +LD LI +SQ+ E
Sbjct: 58 ASWRIITSIEQKEESKGEDMAKLEMIRNYRKTIEEELKTICGEILSLLDDSLIKNSQSEE 117
Query: 463 SKVFYYKM 486
SKVFY K+
Sbjct: 118 SKVFYNKI 125
>SB_34217| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 219
Score = 155 bits (377), Expect = 3e-38
Identities = 73/145 (50%), Positives = 108/145 (74%)
Frame = +1
Query: 106 MSEREDNVYKAKLAEQAERYDEMVEAMKNVASRNVSDNELTVEERNLLSVAYKNVIGARR 285
M RE +Y AKL+EQ +RYDEM + MK V+ + +L+ EERNLLSV+YKN++G RR
Sbjct: 81 MVVRETLIYNAKLSEQCDRYDEMAKIMKEVSEKYP---KLSKEERNLLSVSYKNIVGQRR 137
Query: 286 ASWRIISSIEQKEETKGAEDKLNMIRAYRSQVEKELRDICSDILGVLDKYLIPSSQTGES 465
+SWR+ISSIE+K L +++ Y++ +EKEL+D+C ++LG+L++ LIP ++ E+
Sbjct: 138 SSWRVISSIEEK---TAESSSLAIVKKYKACIEKELKDLCKEVLGILER-LIPGAEDEEN 193
Query: 466 KVFYYKMKGDYHRYLAEFATGNDRK 540
KVFY+K+KGDY+RYLAEF+ G + K
Sbjct: 194 KVFYFKLKGDYYRYLAEFSHGQEHK 218
>SB_34218| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 607
Score = 72.5 bits (170), Expect = 3e-13
Identities = 42/113 (37%), Positives = 64/113 (56%), Gaps = 3/113 (2%)
Frame = +1
Query: 88 NHLSSTMS---EREDNVYKAKLAEQAERYDEMVEAMKNVASRNVSDNELTVEERNLLSVA 258
NH S + R + V AKLAEQ ER+++++ MK N S N+ E RNLLSV
Sbjct: 178 NHPSGVVQMQDSRNELVQLAKLAEQTERFEDVILYMKKAIEINPSLNK---EHRNLLSVG 234
Query: 259 YKNVIGARRASWRIISSIEQKEETKGAEDKLNMIRAYRSQVEKELRDICSDIL 417
YKNV+G++R +WR + + + +L I Y+ ++E EL+ +C +IL
Sbjct: 235 YKNVVGSKRFAWRHLHHDALQSGRYIRDSQLKGIIKYKEKIEMELKTLCREIL 287
>SB_56522| Best HMM Match : Pox_A_type_inc (HMM E-Value=1.6e-30)
Length = 3071
Score = 36.3 bits (80), Expect = 0.025
Identities = 29/114 (25%), Positives = 58/114 (50%), Gaps = 9/114 (7%)
Frame = +1
Query: 118 EDNVYKAKLAEQAERYDEMV----EAMKNVASRNVSDNELTVEE----RNLLSVAYKNVI 273
+ N+ K KL + E Y + + +A +N N N+L +E R+L S+ + N +
Sbjct: 1955 DPNLQK-KLKDLEESYQKALKDNEDAERNYLDLNRKYNKLVMEHGNISRDLESLKHDNDL 2013
Query: 274 GARRA-SWRIISSIEQKEETKGAEDKLNMIRAYRSQVEKELRDICSDILGVLDK 432
R ++ Q++E + A++K+N + AY++ E++LR+ D++ K
Sbjct: 2014 KNERIIDLESSLAVYQEQELQSAKNKINRLEAYKTSYEEKLRNQQEDLVAAQQK 2067
>SB_20371| Best HMM Match : U-box (HMM E-Value=0.075)
Length = 515
Score = 33.5 bits (73), Expect = 0.17
Identities = 22/88 (25%), Positives = 42/88 (47%), Gaps = 3/88 (3%)
Frame = +1
Query: 91 HLSSTMSEREDNVYKAKLAEQAERYDEM---VEAMKNVASRNVSDNELTVEERNLLSVAY 261
H+ M + D V +AK A+ YD + ++ + + + D E +++LL+ +
Sbjct: 345 HMEREMDQWMDAVQQAK-AQFTREYDPAKSEIPDIETIFQKKIEDLESANNDKDLLN--H 401
Query: 262 KNVIGARRASWRIISSIEQKEETKGAED 345
K ++G + W++ EQ GAED
Sbjct: 402 KKIVGFDKKIWKVHHEKEQMIGAGGAED 429
>SB_51222| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 2520
Score = 32.7 bits (71), Expect = 0.30
Identities = 31/139 (22%), Positives = 65/139 (46%), Gaps = 2/139 (1%)
Frame = +1
Query: 130 YKAKLAEQAERYDEMVEAMKNVASRNVSDNELTVEERNLLSVAYKNVIGARRASWRIISS 309
+ KLAE +Y + +E M N A + + + + + +E+ L + K + A++ S + ++
Sbjct: 1460 FNDKLAETQSQYSQDMEEM-NQAKKEL-EIKYSTKEKELRAELEKREVQAKQTSETMTAN 1517
Query: 310 IEQ--KEETKGAEDKLNMIRAYRSQVEKELRDICSDILGVLDKYLIPSSQTGESKVFYYK 483
I Q + E K ++ L + ++ + + S I +++++ SQ
Sbjct: 1518 ISQLNEREKKHKQEMLVLENNWKEKYSEIEERYSSQISNIIEQHQREISQ---------- 1567
Query: 484 MKGDYHRYLAEFATGNDRK 540
+K D+H LAE N +K
Sbjct: 1568 LKSDHHSELAELKAHNQQK 1586
>SB_22570| Best HMM Match : Filament (HMM E-Value=0.1)
Length = 601
Score = 31.5 bits (68), Expect = 0.70
Identities = 29/105 (27%), Positives = 52/105 (49%), Gaps = 4/105 (3%)
Frame = +1
Query: 88 NHLSSTMSEREDNVYKAKLAEQAERYDEMVEAM----KNVASRNVSDNELTVEERNLLSV 255
N L ST++ER + ++ L E R+DE + ++ KN+ R S N+L +N S
Sbjct: 217 NSLVSTLNERLERK-ESTLMECRRRHDEELTSLQKKIKNLEERLSSTNQLISSTKNDESG 275
Query: 256 AYKNVIGARRASWRIISSIEQKEETKGAEDKLNMIRAYRSQVEKE 390
+ +I ++ I+ +E+ + AE +L M A ++E E
Sbjct: 276 RIQALIKRVCLQYKRINRLEETRHQEKAEMELKM--AQLKEIENE 318
>SB_12729| Best HMM Match : Pox_A_type_inc (HMM E-Value=5.3e-08)
Length = 1183
Score = 30.7 bits (66), Expect = 1.2
Identities = 26/109 (23%), Positives = 50/109 (45%), Gaps = 1/109 (0%)
Frame = +1
Query: 88 NHLSSTMSERED-NVYKAKLAEQAERYDEMVEAMKNVASRNVSDNELTVEERNLLSVAYK 264
N ++ ER D +V L Q R + + MK R+ ++ + +E++ L A
Sbjct: 430 NSVNVLRKERVDLDVQVTNLRSQKIRLEAEISVMKEERRRDDNEVDHLRKEKSDLEKAMH 489
Query: 265 NVIGARRASWRIISSIEQKEETKGAEDKLNMIRAYRSQVEKELRDICSD 411
++ R ++ + I E+T + + +R +EKELRD+ S+
Sbjct: 490 SL---RSEKTKVETDITMAEDTWRKRNDTSELRFEADDLEKELRDLRSE 535
>SB_7846| Best HMM Match : CoCoA (HMM E-Value=0.00016)
Length = 1284
Score = 30.7 bits (66), Expect = 1.2
Identities = 22/92 (23%), Positives = 48/92 (52%), Gaps = 2/92 (2%)
Frame = +1
Query: 130 YKAKLAEQAERYDEMVEAMKNVASRNVSDNELTVEERNLLSVAYKNVIGARRASWRIISS 309
YK K E + ++ +A ++ S N SDNE+ + RN +S + + R +S +
Sbjct: 1024 YKIKFIEWRKLQSQLQKARRSSPSVN-SDNEVCHDARNTISKLREEMEELRESSAAAQKA 1082
Query: 310 IEQKEETKGAEDKLN--MIRAYRSQVEKELRD 399
+EQ+++ + + ++N I R +++K + +
Sbjct: 1083 LEQEKQQRLEKLEMNNQEIMFLREELQKAVEE 1114
>SB_43756| Best HMM Match : SlyX (HMM E-Value=3.7)
Length = 179
Score = 29.9 bits (64), Expect = 2.1
Identities = 16/80 (20%), Positives = 39/80 (48%), Gaps = 1/80 (1%)
Frame = +1
Query: 94 LSSTMSEREDNVYKAKLAEQAERYDEMVEAMKNVASRNVSDNELTVEER-NLLSVAYKNV 270
LSS +SER + + +L + E Y ++ ++ R+ +L ++ L+++++
Sbjct: 53 LSSVLSERCGELERRQLEMEREFYTKIQALEAQISDRSEEWKDLLAQDHAKLMTISHDTT 112
Query: 271 IGARRASWRIISSIEQKEET 330
+ W++ + KE+T
Sbjct: 113 YDLKSGYWQVEMDPKDKEKT 132
>SB_36640| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 283
Score = 29.5 bits (63), Expect = 2.8
Identities = 18/47 (38%), Positives = 20/47 (42%)
Frame = -1
Query: 250 TAGSSPPLSAHCRTRCVMLHSSSPPPFRHSAQPAQLTLLYTHYLPFP 110
TA S PP +A C T C + P P P T L H LP P
Sbjct: 40 TALSPPPATALCPTPC---YGPVPHPLLRPCVPPPATALVPHPLPRP 83
>SB_37483| Best HMM Match : Drf_FH1 (HMM E-Value=6.6)
Length = 237
Score = 28.7 bits (61), Expect = 4.9
Identities = 17/48 (35%), Positives = 21/48 (43%), Gaps = 1/48 (2%)
Frame = -1
Query: 190 SSSPPPFRH-SAQPAQLTLLYTHYLPFPTLWMTNDFTLTKSESPGHLF 50
+S P P+ S QPA T YT Y P T+ T + SP F
Sbjct: 73 ASQPAPYTPASGQPASYTPAYTPYTPASTVGRMPPTTSQSTPSPAPFF 120
>SB_24031| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 1176
Score = 28.7 bits (61), Expect = 4.9
Identities = 16/46 (34%), Positives = 22/46 (47%)
Frame = +1
Query: 481 KMKGDYHRYLAEFATGNDRKEAAENSLVAYKAASDIAMTELPPTHP 618
++K Y ++L +F KE AENS A A+ A PP P
Sbjct: 859 EVKEAYRKWLDDFMKSAREKEIAENSRYASTQAATSAPYASPPPSP 904
>SB_2776| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 1792
Score = 28.3 bits (60), Expect = 6.5
Identities = 17/53 (32%), Positives = 29/53 (54%)
Frame = +1
Query: 103 TMSEREDNVYKAKLAEQAERYDEMVEAMKNVASRNVSDNELTVEERNLLSVAY 261
T+SERE KA++ A E A ++A + ++NE +E R+L +A+
Sbjct: 788 TISEREAIANKARIRSGALTLSETGPAFVDLALKVPAENEEKLENRDLEEMAW 840
>SB_29939| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 1122
Score = 28.3 bits (60), Expect = 6.5
Identities = 13/43 (30%), Positives = 22/43 (51%)
Frame = +1
Query: 121 DNVYKAKLAEQAERYDEMVEAMKNVASRNVSDNELTVEERNLL 249
D VY+ L RY+E+V + N + +N TV +R ++
Sbjct: 80 DEVYRPYLTTDETRYNEVVFVVDNTVYQRYHNNTKTVTDRVII 122
>SB_11774| Best HMM Match : M (HMM E-Value=8.5e-09)
Length = 1998
Score = 28.3 bits (60), Expect = 6.5
Identities = 33/147 (22%), Positives = 68/147 (46%), Gaps = 3/147 (2%)
Frame = +1
Query: 112 EREDNVYKAKLAEQAERYDEMVEAMKNVASRNVSDNELTVEERNL--LSVAYKNVIGARR 285
E+ N ++ L + ++Y+E++EA++ S+ VEER L L+ A +G
Sbjct: 1651 EKMKNDHEQDLTSERKKYEEIIEALQGELSK-AEVRLKEVEERLLSALAEAKSQKLGNDP 1709
Query: 286 ASWRIISSIEQKEETKGAEDKLNM-IRAYRSQVEKELRDICSDILGVLDKYLIPSSQTGE 462
S I + K KG E + + + A + + E+ L+ + ++ + +K I +
Sbjct: 1710 ESPTSIDESDIKLIKKGLEFEYDQKLSAEKRKFEETLQGLRKEVGNLQEKRKII-----Q 1764
Query: 463 SKVFYYKMKGDYHRYLAEFATGNDRKE 543
+V+ + G R++ E + N + E
Sbjct: 1765 ERVYNQESFGAVDRHVIEKSIANYKME 1791
>SB_47663| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 1409
Score = 27.9 bits (59), Expect = 8.6
Identities = 13/40 (32%), Positives = 19/40 (47%)
Frame = +1
Query: 121 DNVYKAKLAEQAERYDEMVEAMKNVASRNVSDNELTVEER 240
D VY+ L RY EMV + N + +N TV ++
Sbjct: 170 DEVYRPYLTTDETRYTEMVFVVDNTVYQRYQNNTKTVTDK 209
>SB_44921| Best HMM Match : Pepsin-I3 (HMM E-Value=6.4)
Length = 105
Score = 27.9 bits (59), Expect = 8.6
Identities = 18/84 (21%), Positives = 36/84 (42%)
Frame = +1
Query: 88 NHLSSTMSEREDNVYKAKLAEQAERYDEMVEAMKNVASRNVSDNELTVEERNLLSVAYKN 267
N + ER + ++ + +D ++ NV R + L + L+ A
Sbjct: 21 NKTAELYKERAKALSRSPEETRYSSFDTSLDYNGNVLHRGDLEASLNHQLTEKLAEAESE 80
Query: 268 VIGARRASWRIISSIEQKEETKGA 339
++ +R + +IS +EQK +GA
Sbjct: 81 ILASRMSRDNLISQVEQKIAARGA 104
>SB_39167| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 960
Score = 27.9 bits (59), Expect = 8.6
Identities = 18/84 (21%), Positives = 36/84 (42%)
Frame = +1
Query: 88 NHLSSTMSEREDNVYKAKLAEQAERYDEMVEAMKNVASRNVSDNELTVEERNLLSVAYKN 267
N + ER + ++ + +D ++ NV R + L + L+ A
Sbjct: 98 NKTAELYKERAKALSRSPEETRYSSFDTSLDYNGNVLHRGDLEASLNHQLTEKLAEAESE 157
Query: 268 VIGARRASWRIISSIEQKEETKGA 339
++ +R + +IS +EQK +GA
Sbjct: 158 ILASRMSRDNLISQVEQKIAARGA 181
>SB_34997| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 845
Score = 27.9 bits (59), Expect = 8.6
Identities = 16/47 (34%), Positives = 25/47 (53%)
Frame = -3
Query: 443 GMRYLSRTPRMSEQMSRSSFSTWLRYALIMFNLSSAPFVSSFCSMEE 303
G R RTP ++ + S +W YALI+F+L F+ C++ E
Sbjct: 77 GSRLKGRTPHLTRALM--SILSWSEYALILFSL----FIGVACNILE 117
Database: nematostella
Posted date: Oct 22, 2007 1:22 PM
Number of letters in database: 16,821,457
Number of sequences in database: 59,808
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 19,170,545
Number of Sequences: 59808
Number of extensions: 376695
Number of successful extensions: 1193
Number of sequences better than 10.0: 22
Number of HSP's better than 10.0 without gapping: 1082
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1180
length of database: 16,821,457
effective HSP length: 80
effective length of database: 12,036,817
effective search space used: 1877743452
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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