Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= bmmt10h21
         (700 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC29A4.02c |||translation elongation factor EF-1 gamma subunit...    78   1e-15
SPCC1183.02 |||glutathione S-transferase |Schizosaccharomyces po...    66   3e-12
SPAC30C2.04 |||cofactor for methionyl-and glutamyl-tRNA syntheta...    34   0.017
SPAC16.05c |sfp1||transcription factor Sfp1 |Schizosaccharomyces...    28   1.1  
SPAPB1E7.06c |eme1||Holliday junction resolvase subunit Eme1|Sch...    27   2.6  
SPAC14C4.04 |B22918-2||hypothetical protein|Schizosaccharomyces ...    27   2.6  
SPBC28F2.09 |||transcription factor TFIIA complex large subunit ...    26   4.5  
SPBC16A3.11 |eso1||sister chromatid cohesion protein Eso1|Schizo...    26   4.5  
SPAC15F9.02 |seh1||nucleoporin Seh1 |Schizosaccharomyces pombe|c...    26   6.0  
SPBC1773.10c |||asparagine-tRNA ligase Ded81 |Schizosaccharomyce...    25   7.9  

>SPAC29A4.02c |||translation elongation factor EF-1 gamma subunit
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 409

 Score = 78.2 bits (184), Expect = 1e-15
 Identities = 54/190 (28%), Positives = 94/190 (49%), Gaps = 4/190 (2%)
 Frame = +2

Query: 143 NKSEDFLKKFPAGKVPAFESADGKVLLTESNAIAYYVAN----ESLRGGDLATQARVWQW 310
           N   D   KFP  K+P F   DG   L+E+ AIA+Y+A+     +L G     +A+V Q+
Sbjct: 38  NFPADLAAKFPLQKMPVFVGKDG-FPLSETLAIAFYLASLNKTRALNGTTAEEKAKVLQY 96

Query: 311 ASWSDSELLPASCAWVFPYLGIMQFNKQNVERAKSDLLAALKVLDGHLLTRTFLVTERIT 490
            S+++SEL  A    + P +    +++Q  + A++ +       D  L ++T+LV  R+T
Sbjct: 97  CSFTNSELPGAFRPIIAPRVFGAPYDEQAAKEAETAIALIFARFDEELASKTYLVGSRLT 156

Query: 491 LADVIVFSTLLHAFQHVLDPSVRSSLINVQRWFLTVAHQPQVSAVVGSLTLCAAPPTYDP 670
           LAD+     L     +VL  S  +   ++ R++ T+ HQ ++ A+   L     P     
Sbjct: 157 LADIFFTCFLKFGATYVLTKSYLAKYTHIYRYYQTIYHQAKLDAITEPLKFIDQPLPIIK 216

Query: 671 KKYQELAGAQ 700
            + +E A A+
Sbjct: 217 AENKEAAPAK 226


>SPCC1183.02 |||glutathione S-transferase |Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 220

 Score = 66.5 bits (155), Expect = 3e-12
 Identities = 51/206 (24%), Positives = 95/206 (46%), Gaps = 8/206 (3%)
 Frame = +2

Query: 23  MAAGVLYTYPENFRAYKALIAAQYSGTDVKVAPNFVFGETNKSEDFLKKFPAGKVPAFES 202
           M  G LY++  N R    L  A+     V +   +       S D   KFP  K+P F  
Sbjct: 1   MFLGTLYSFKTNTRTVCLLELAKLLDLQVDLVETYPH---KFSADLAAKFPLQKLPVFIG 57

Query: 203 ADGKVLLTESNAIAYYVAN------ESLRGGDLATQARVWQWASWSDSELL-PASCA-WV 358
           ADG  L      + Y+         E L   +   +A + +W  + + +++ P +   WV
Sbjct: 58  ADGFELSEVIAIVKYFYEKGKHNDKEGLGPVNEVEEAEMLKWMCFINFDIVTPQNVRPWV 117

Query: 359 FPYLGIMQFNKQNVERAKSDLLAALKVLDGHLLTRTFLVTERITLADVIVFSTLLHAFQH 538
             + G + + ++  + + +  + +LK+ +  +  RT+LV +R TLAD+   S L   F  
Sbjct: 118 GMFRGNIPYEEKPFKESATRAIDSLKIPNELVKDRTYLVGDRFTLADLFFGSLLRIFFNS 177

Query: 539 VLDPSVRSSLINVQRWFLTVAHQPQV 616
           ++D   R  L ++ R+++T+ HQ ++
Sbjct: 178 IIDEKTRKELPHLTRYYITMFHQAKL 203


>SPAC30C2.04 |||cofactor for methionyl-and glutamyl-tRNA synthetases
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 450

 Score = 34.3 bits (75), Expect = 0.017
 Identities = 24/82 (29%), Positives = 40/82 (48%), Gaps = 1/82 (1%)
 Frame = +2

Query: 440 LDGHLLTRTFLVTER-ITLADVIVFSTLLHAFQHVLDPSVRSSLINVQRWFLTVAHQPQV 616
           LD  L + TF+  +  I++AD+ V++ + H++   L       L NV RWF  + HQ  V
Sbjct: 128 LDEFLKSSTFIAQDSGISVADLAVYARI-HSYICGLSAKEGYKLNNVCRWFDFIQHQESV 186

Query: 617 SAVVGSLTLCAAPPTYDPKKYQ 682
                S+++  A    +  K Q
Sbjct: 187 MEAANSMSMKLANIDLNAPKIQ 208


>SPAC16.05c |sfp1||transcription factor Sfp1 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 442

 Score = 28.3 bits (60), Expect = 1.1
 Identities = 13/31 (41%), Positives = 17/31 (54%)
 Frame = +3

Query: 417 TYWPP*KYWTDIFSHAPSLLPRESHLPMSLS 509
           TY PP  Y  D+FS APS+   +     S+S
Sbjct: 257 TYLPPFNYDHDVFSFAPSVASADQFTESSMS 287


>SPAPB1E7.06c |eme1||Holliday junction resolvase subunit
           Eme1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 738

 Score = 27.1 bits (57), Expect = 2.6
 Identities = 16/62 (25%), Positives = 30/62 (48%)
 Frame = +2

Query: 50  PENFRAYKALIAAQYSGTDVKVAPNFVFGETNKSEDFLKKFPAGKVPAFESADGKVLLTE 229
           P  F++ KA +  QY+          +FG  +K ++F K+    ++  F S +  +L+  
Sbjct: 553 PNYFKSLKAELNRQYAAAVNSGTRPLLFGSLSKYQNFTKEKLESEIVRF-SFEHSILINT 611

Query: 230 SN 235
           SN
Sbjct: 612 SN 613


>SPAC14C4.04 |B22918-2||hypothetical protein|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 267

 Score = 27.1 bits (57), Expect = 2.6
 Identities = 13/33 (39%), Positives = 17/33 (51%), Gaps = 1/33 (3%)
 Frame = +3

Query: 279 IWLPKPVSGSGHHGLTANYCL-LPALGSSLTLV 374
           +W+P PV  SG    T   C+ LP  G  +T V
Sbjct: 23  LWVPTPVGRSGRKHRTLKMCIVLPDTGYDVTQV 55


>SPBC28F2.09 |||transcription factor TFIIA complex large subunit
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 369

 Score = 26.2 bits (55), Expect = 4.5
 Identities = 12/25 (48%), Positives = 14/25 (56%)
 Frame = -2

Query: 219 STFPSALSNAGTFPAGNFFKKSSDL 145
           +TFP A +  GTFP G  F   S L
Sbjct: 52  ATFPWAQAPVGTFPIGQLFDPVSGL 76


>SPBC16A3.11 |eso1||sister chromatid cohesion protein
           Eso1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 872

 Score = 26.2 bits (55), Expect = 4.5
 Identities = 12/29 (41%), Positives = 18/29 (62%)
 Frame = +2

Query: 230 SNAIAYYVANESLRGGDLATQARVWQWAS 316
           S + A Y+ NE+LR G +   +R+W  AS
Sbjct: 780 STSSAVYIKNENLRKGFVLGISRIWVSAS 808


>SPAC15F9.02 |seh1||nucleoporin Seh1 |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 339

 Score = 25.8 bits (54), Expect = 6.0
 Identities = 14/39 (35%), Positives = 22/39 (56%), Gaps = 3/39 (7%)
 Frame = +3

Query: 309 GHHGLTANYCLLPALGSSLTLV--SCNSTN-RMLNVQSL 416
           GH  L  + C  P++GSS  L+  +C   N R+  V++L
Sbjct: 213 GHTDLIRDICWAPSMGSSYYLIATACKDGNVRIFKVETL 251


>SPBC1773.10c |||asparagine-tRNA ligase Ded81 |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 568

 Score = 25.4 bits (53), Expect = 7.9
 Identities = 19/48 (39%), Positives = 25/48 (52%), Gaps = 2/48 (4%)
 Frame = +2

Query: 2   LFPKTPTMAAGVLYTYPENFRAYKALIAAQYSG-TDVKVAPNFV-FGE 139
           L+ +    A G +YT  E+FRA K+L     S  T V+    FV FGE
Sbjct: 321 LYLEAALPALGSVYTIQESFRAEKSLTRRHLSEFTHVEFELPFVNFGE 368


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,075,871
Number of Sequences: 5004
Number of extensions: 65579
Number of successful extensions: 223
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 216
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 221
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 323158234
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)