BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= bmmt10g18 (426 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC970.05 |rpl3601|rpl36-1|60S ribosomal protein L36|Schizosacc... 85 6e-18 SPBC405.07 |rpl3602|rpl36-2, rpl36|60S ribosomal protein L36|Sch... 84 8e-18 SPBP4H10.11c |||long-chain-fatty-acid-CoA ligase |Schizosaccharo... 28 0.70 SPAC21E11.06 |tif224||translation initiation factor eIF2B delta ... 27 0.92 SPBC36B7.09 |gcn2|ppk28, ppk28, SPBP18G5.01|eIF2 alpha kinase Gc... 27 0.92 SPAC22G7.02 |kap111||karyopherin Kap111|Schizosaccharomyces pomb... 27 1.2 SPCC74.03c |ssp2|ucp9|serine/threonine protein kinase Ssp2|Schiz... 26 2.8 SPAPB1A10.06c |||ATP-dependent RNA helicase Dhr1 |Schizosaccharo... 25 3.7 SPCC306.09c |cap1|cap|adenylyl cyclase-associated protein Cap1|S... 25 4.9 SPBC3E7.12c |chr1|cfh4|chitin synthase regulatory factor |Schizo... 25 4.9 SPAC227.13c |isu1||mitochondrial iron-sulfur cluster assembly sc... 25 4.9 SPAC13F5.01c |msh1|SPAC23C11.18c|MutS protein homolog 1|Schizosa... 25 4.9 >SPCC970.05 |rpl3601|rpl36-1|60S ribosomal protein L36|Schizosaccharomyces pombe|chr 3|||Manual Length = 99 Score = 84.6 bits (200), Expect = 6e-18 Identities = 43/84 (51%), Positives = 58/84 (69%) Frame = +2 Query: 104 KGITDKAIRIRPARLKGLQTKHSKFVRDLVREVVGHAQYEKRAMELLKVSKDKRALKFLK 283 K +T + + RP+R KG +K + FVR +VREV G A YE+R MEL++ S+DKRA K K Sbjct: 13 KVLTKRQLPERPSRRKGQLSKRTSFVRSIVREVAGFAPYERRVMELIRNSQDKRARKLAK 72 Query: 284 RRLGTHIRAKRKREELSNVLAQMR 355 +RLGT RAK K EEL++V+ R Sbjct: 73 KRLGTLKRAKGKIEELTSVIQSSR 96 >SPBC405.07 |rpl3602|rpl36-2, rpl36|60S ribosomal protein L36|Schizosaccharomyces pombe|chr 2|||Manual Length = 99 Score = 84.2 bits (199), Expect = 8e-18 Identities = 43/84 (51%), Positives = 58/84 (69%) Frame = +2 Query: 104 KGITDKAIRIRPARLKGLQTKHSKFVRDLVREVVGHAQYEKRAMELLKVSKDKRALKFLK 283 K +T + + RP+R KG +K + FVR +VREV G A YE+R MEL++ S+DKRA K K Sbjct: 13 KTLTKRQLPERPSRRKGHLSKRTAFVRSIVREVAGFAPYERRVMELIRNSQDKRARKLAK 72 Query: 284 RRLGTHIRAKRKREELSNVLAQMR 355 +RLGT RAK K EEL++V+ R Sbjct: 73 KRLGTLKRAKGKIEELTSVIQSSR 96 >SPBP4H10.11c |||long-chain-fatty-acid-CoA ligase |Schizosaccharomyces pombe|chr 2|||Manual Length = 689 Score = 27.9 bits (59), Expect = 0.70 Identities = 14/42 (33%), Positives = 24/42 (57%) Frame = -3 Query: 382 MVMSLGGCLPHLSEHVAKFFTLPLGADVCAQSSLQELQSTLV 257 +V SL G L + A+FFT PL ++VCA + + + ++ Sbjct: 544 IVKSLNGEYIALEKIEAQFFTSPLVSNVCAYADVNHAKPVVI 585 >SPAC21E11.06 |tif224||translation initiation factor eIF2B delta subunit|Schizosaccharomyces pombe|chr 1|||Manual Length = 467 Score = 27.5 bits (58), Expect = 0.92 Identities = 13/24 (54%), Positives = 16/24 (66%) Frame = +3 Query: 318 SVKNLATCSLR*GRQPPRLITITI 389 SVKNL SL+ PPRLIT+ + Sbjct: 421 SVKNLKLLSLKYDVTPPRLITVCV 444 >SPBC36B7.09 |gcn2|ppk28, ppk28, SPBP18G5.01|eIF2 alpha kinase Gcn2 |Schizosaccharomyces pombe|chr 2|||Manual Length = 1576 Score = 27.5 bits (58), Expect = 0.92 Identities = 19/53 (35%), Positives = 28/53 (52%), Gaps = 1/53 (1%) Frame = +2 Query: 200 VVGHAQYEKRAMELLKVSKDKRALKFLKRRLGTHIRAK-RKREELSNVLAQMR 355 ++ HA ++ L+VSKD KRR+ THI + +R LS V Q+R Sbjct: 1119 LINHADILSSILDYLQVSKD-------KRRMATHILGQINQRLTLSQVRNQLR 1164 >SPAC22G7.02 |kap111||karyopherin Kap111|Schizosaccharomyces pombe|chr 1|||Manual Length = 990 Score = 27.1 bits (57), Expect = 1.2 Identities = 14/37 (37%), Positives = 26/37 (70%), Gaps = 3/37 (8%) Frame = +1 Query: 190 STRSCRTRSI*EEGY---GVT*GVKRQACSEVLEATI 291 +TR+C T+S+ EEG+ V+ +KR+ +++L+A I Sbjct: 928 ATRACLTQSLEEEGFPSRNVSNEIKRRFLTDLLKARI 964 >SPCC74.03c |ssp2|ucp9|serine/threonine protein kinase Ssp2|Schizosaccharomyces pombe|chr 3|||Manual Length = 576 Score = 25.8 bits (54), Expect = 2.8 Identities = 14/48 (29%), Positives = 26/48 (54%), Gaps = 4/48 (8%) Frame = +2 Query: 188 LVREVVGHAQYEKRAMELLKVSKDKRALKFLKRRL----GTHIRAKRK 319 ++RE +G + K + ++ K ALKF+ R+L H+R +R+ Sbjct: 35 IIRETLGEGSFGKVKLATHYKTQQKVALKFISRQLLKKSDMHMRVERE 82 >SPAPB1A10.06c |||ATP-dependent RNA helicase Dhr1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1183 Score = 25.4 bits (53), Expect = 3.7 Identities = 13/39 (33%), Positives = 27/39 (69%) Frame = +2 Query: 236 ELLKVSKDKRALKFLKRRLGTHIRAKRKREELSNVLAQM 352 +LLK ++ + K K+RL +I K K+EE+++++A++ Sbjct: 75 QLLKENEGSISSK-KKKRLDKYIENKLKKEEVASLIAKL 112 >SPCC306.09c |cap1|cap|adenylyl cyclase-associated protein Cap1|Schizosaccharomyces pombe|chr 3|||Manual Length = 551 Score = 25.0 bits (52), Expect = 4.9 Identities = 17/65 (26%), Positives = 30/65 (46%), Gaps = 1/65 (1%) Frame = -3 Query: 403 FIYHLMVMVMSLGGCLPHLSEHVAKFFTLPLGA-DVCAQSSLQELQSTLVF*HLK*LHSP 227 ++ M + +GG + SEHV K F L V ++ ++ S + LK + S Sbjct: 116 YLSKYMELSKKIGGLIAEQSEHVEKAFNLLRQVLSVALKAQKPDMDSPELLEFLKPIQSE 175 Query: 226 LLILS 212 LL ++ Sbjct: 176 LLTIT 180 >SPBC3E7.12c |chr1|cfh4|chitin synthase regulatory factor |Schizosaccharomyces pombe|chr 2|||Manual Length = 456 Score = 25.0 bits (52), Expect = 4.9 Identities = 8/22 (36%), Positives = 17/22 (77%) Frame = -1 Query: 261 LSFDTLSNSIALFSY*ACPTTS 196 +S+D+LS+ +++ S+ CP T+ Sbjct: 79 MSYDSLSDDVSVLSFLDCPLTT 100 >SPAC227.13c |isu1||mitochondrial iron-sulfur cluster assembly scaffold protein Isu1|Schizosaccharomyces pombe|chr 1|||Manual Length = 192 Score = 25.0 bits (52), Expect = 4.9 Identities = 13/35 (37%), Positives = 18/35 (51%) Frame = +2 Query: 134 RPARLKGLQTKHSKFVRDLVREVVGHAQYEKRAME 238 RPA L+ L+T SKFV + V Y K ++ Sbjct: 27 RPANLQFLKTNSSKFVPQVTAN-VSRRMYHKNVLD 60 >SPAC13F5.01c |msh1|SPAC23C11.18c|MutS protein homolog 1|Schizosaccharomyces pombe|chr 1|||Manual Length = 941 Score = 25.0 bits (52), Expect = 4.9 Identities = 12/29 (41%), Positives = 19/29 (65%) Frame = -1 Query: 249 TLSNSIALFSY*ACPTTSRTKSRTNLECF 163 T+++S LF+ A T+ TKS N+EC+ Sbjct: 850 TINHSRTLFATHAHQLTNLTKSFKNVECY 878 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,551,699 Number of Sequences: 5004 Number of extensions: 27991 Number of successful extensions: 77 Number of sequences better than 10.0: 12 Number of HSP's better than 10.0 without gapping: 77 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 77 length of database: 2,362,478 effective HSP length: 66 effective length of database: 2,032,214 effective search space used: 152416050 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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