BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= bmmt10e23 (673 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g07400.1 68414.m00789 17.8 kDa class I heat shock protein (HS... 36 0.025 At4g27670.1 68417.m03979 25.3 kDa small heat shock protein, chlo... 35 0.043 At2g22430.1 68415.m02660 homeobox-leucine zipper protein 6 (HB-6... 29 3.7 At1g55970.1 68414.m06419 histone acetyltransferase 4 (HAC4) simi... 29 3.7 At5g54110.1 68418.m06737 vesicle-associated membrane family prot... 28 4.9 At5g45270.1 68418.m05556 WRKY family transcription factor contai... 28 6.5 At1g79940.1 68414.m09342 DNAJ heat shock N-terminal domain-conta... 28 6.5 At1g59750.2 68414.m06728 auxin-responsive factor (ARF1) identica... 28 6.5 At1g59750.1 68414.m06727 auxin-responsive factor (ARF1) identica... 28 6.5 At3g61415.1 68416.m06878 SKP1 family protein low similarity to S... 27 8.6 >At1g07400.1 68414.m00789 17.8 kDa class I heat shock protein (HSP17.8-CI) similar to 17.5 kDa class I heat shock protein SP:P04793 from [Glycine max]; contains Pfam PF00011: Hsp20/alpha crystallin family Length = 157 Score = 35.9 bits (79), Expect = 0.025 Identities = 26/77 (33%), Positives = 42/77 (54%), Gaps = 9/77 (11%) Frame = +2 Query: 425 DVSQYTPEEIVVKTVDNKLLV-----HAKHEEKSDT-KSVYR---EYNREFLLPKGTNPE 577 D+ EE+ V+ D+ +L H + EEK DT V R +++R+F LP+ + Sbjct: 64 DLPGMKKEEVKVEIEDDSVLKISGERHVEKEEKQDTWHRVERSSGQFSRKFKLPENVKMD 123 Query: 578 AIKSSLSRDGVLTVEAP 628 +K+S+ +GVLTV P Sbjct: 124 QVKASM-ENGVLTVTVP 139 >At4g27670.1 68417.m03979 25.3 kDa small heat shock protein, chloroplast precursor (HSP25.3-P) identical to small heat shock protein, chloroplast precursor SP:P31170 from [Arabidopsis thaliana]; identified in Scharf, K-D., et al,Cell Stress & Chaperones (2001) 6: 225-237. Length = 227 Score = 35.1 bits (77), Expect = 0.043 Identities = 22/77 (28%), Positives = 38/77 (49%), Gaps = 4/77 (5%) Frame = +2 Query: 410 LKLRFDVSQYTPEEIVVKTVDNKLLV--HAKHEEKSDTKS--VYREYNREFLLPKGTNPE 577 +K+RFD+ + E++ + DN L++ K E+ D+ S Y LP + Sbjct: 138 IKMRFDMPGLSKEDVKISVEDNVLVIKGEQKKEDSDDSWSGRSVSSYGTRLQLPDNCEKD 197 Query: 578 AIKSSLSRDGVLTVEAP 628 IK+ L ++GVL + P Sbjct: 198 KIKAEL-KNGVLFITIP 213 >At2g22430.1 68415.m02660 homeobox-leucine zipper protein 6 (HB-6) / HD-ZIP transcription factor 6 identical to homeobox-leucine zipper protein ATHB-6 (HD-ZIP protein ATHB-6) (SP:P46668) [Arabidopsis thaliana] Length = 311 Score = 28.7 bits (61), Expect = 3.7 Identities = 13/37 (35%), Positives = 24/37 (64%), Gaps = 3/37 (8%) Frame = +2 Query: 161 DFSVIDTEFSSIRERFDAEMRKME---EEMSKFRSEL 262 D+ V+ T++ S+R FD+ R E +E+SK +++L Sbjct: 122 DYGVLKTQYDSLRHNFDSLRRDNESLLQEISKLKTKL 158 >At1g55970.1 68414.m06419 histone acetyltransferase 4 (HAC4) similar to CREB-binding protein GB:AAC51770 GI:2443859 from [Homo sapiens]; contains Pfam PF02135: TAZ zinc finger profile; contains Pfam PF00569: Zinc finger, ZZ type domain; identical to histone acetyltransferase HAC4 (GI:14794966) {Arabidopsis thaliana} Length = 1456 Score = 28.7 bits (61), Expect = 3.7 Identities = 18/40 (45%), Positives = 22/40 (55%), Gaps = 1/40 (2%) Frame = +3 Query: 396 VTARLSSFALMSASILPKRSLLR-LSTTNYWSMPNTRRNL 512 VT+ S+ M + LPKR L L TN+ S PN R NL Sbjct: 315 VTSYHSNLQPMQQTPLPKRQLHHPLWNTNFQSAPNNRDNL 354 >At5g54110.1 68418.m06737 vesicle-associated membrane family protein / VAMP family protein similar to VAP27 GI:6688926 [Nicotiana plumbaginifolia] Length = 266 Score = 28.3 bits (60), Expect = 4.9 Identities = 21/67 (31%), Positives = 33/67 (49%), Gaps = 3/67 (4%) Frame = +3 Query: 426 MSASILPKRSLLRLSTTNYWSMPNTRRNLIRN-LCTENTTGSFCCPREQILRPLS--LRC 596 ++ S+LP R LRL ++Y P +R+ + +NT+ S + Q P S +R Sbjct: 66 VARSLLPARRRLRLDPSSYLYFPYEPGKQVRSAIKLKNTSKSHTAFKFQTTAPKSCYMRP 125 Query: 597 PGTVCLP 617 PG V P Sbjct: 126 PGGVLAP 132 >At5g45270.1 68418.m05556 WRKY family transcription factor contains Pfam profile: PF03106 WRKY DNA -binding domain Length = 114 Score = 27.9 bits (59), Expect = 6.5 Identities = 10/28 (35%), Positives = 16/28 (57%) Frame = -1 Query: 646 DGELWQWRFHGKHTVPGQRRLNGLRICS 563 +G+LW WR +G+ + G R G C+ Sbjct: 33 EGDLWTWRKYGQKDILGSRFPRGYYRCA 60 >At1g79940.1 68414.m09342 DNAJ heat shock N-terminal domain-containing protein / sec63 domain-containing protein similar to SP|Q9UGP8 Translocation protein SEC63 homolog {Homo sapiens}; contains Pfam profiles PF00226 DnaJ domain, PF02889 Sec63 domain Length = 702 Score = 27.9 bits (59), Expect = 6.5 Identities = 10/36 (27%), Positives = 21/36 (58%) Frame = +2 Query: 143 IPIKLGDFSVIDTEFSSIRERFDAEMRKMEEEMSKF 250 IP++ D + F S+R + +++ M++E +KF Sbjct: 258 IPVRRTDDEPLQKLFMSVRSELNLDLKNMKQEQAKF 293 >At1g59750.2 68414.m06728 auxin-responsive factor (ARF1) identical to auxin response factor 1 GI:2245378 from [Arabidopsis thaliana] Length = 662 Score = 27.9 bits (59), Expect = 6.5 Identities = 12/28 (42%), Positives = 19/28 (67%) Frame = +2 Query: 437 YTPEEIVVKTVDNKLLVHAKHEEKSDTK 520 YTPEE+ + NKL V+A+ + K+D + Sbjct: 619 YTPEEVKKLSPKNKLAVNARMQLKADAE 646 >At1g59750.1 68414.m06727 auxin-responsive factor (ARF1) identical to auxin response factor 1 GI:2245378 from [Arabidopsis thaliana] Length = 665 Score = 27.9 bits (59), Expect = 6.5 Identities = 12/28 (42%), Positives = 19/28 (67%) Frame = +2 Query: 437 YTPEEIVVKTVDNKLLVHAKHEEKSDTK 520 YTPEE+ + NKL V+A+ + K+D + Sbjct: 622 YTPEEVKKLSPKNKLAVNARMQLKADAE 649 >At3g61415.1 68416.m06878 SKP1 family protein low similarity to SP|P52285 Glycoprotein FP21 precursor {Dictyostelium discoideum}; contains Pfam profile PF01466: Skp1 family, dimerisation domain Length = 351 Score = 27.5 bits (58), Expect = 8.6 Identities = 11/23 (47%), Positives = 17/23 (73%) Frame = -1 Query: 592 RRLNGLRICSLGQQKLPVVFSVH 524 RRLN + S+GQ++ PV FS++ Sbjct: 313 RRLNSSWVLSIGQERQPVNFSIN 335 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 13,667,553 Number of Sequences: 28952 Number of extensions: 278629 Number of successful extensions: 1054 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 1002 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 1054 length of database: 12,070,560 effective HSP length: 78 effective length of database: 9,812,304 effective search space used: 1422784080 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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