Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmmt10b02
(711 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g00370.1 68417.m00051 sugar transporter family protein contai... 75 4e-14
At5g44370.1 68418.m05433 transporter-related similar to vesicula... 70 1e-12
At2g29650.2 68415.m03604 inorganic phosphate transporter, putati... 69 4e-12
At2g29650.1 68415.m03603 inorganic phosphate transporter, putati... 69 4e-12
At2g29650.3 68415.m03602 inorganic phosphate transporter, putati... 67 1e-11
At3g46980.2 68416.m05102 transporter-related low similarity to b... 60 1e-09
At3g46980.1 68416.m05101 transporter-related low similarity to b... 60 1e-09
At5g20380.1 68418.m02424 transporter-related low similarity to v... 57 1e-08
At2g38060.1 68415.m04672 transporter-related low similarity to v... 53 2e-07
At3g13050.1 68416.m01626 transporter-related low similarity to a... 37 0.011
At5g26800.1 68418.m03197 expressed protein predicted protein, Ar... 32 0.33
At1g68900.1 68414.m07885 mandelate racemase/muconate lactonizing... 31 0.75
At1g75710.1 68414.m08795 zinc finger (C2H2 type) family protein ... 30 1.7
At4g28080.1 68417.m04027 expressed protein 28 7.0
At3g19610.1 68416.m02486 hypothetical protein 28 7.0
At3g49840.1 68416.m05449 proline-rich family protein contains pr... 27 9.3
At2g13100.1 68415.m01436 glycerol-3-phosphate transporter, putat... 27 9.3
>At4g00370.1 68417.m00051 sugar transporter family protein contains
Pfam profile PF00083: major facilitator superfamily
protein
Length = 541
Score = 75.4 bits (177), Expect = 4e-14
Identities = 45/138 (32%), Positives = 66/138 (47%)
Frame = +3
Query: 225 LCSNVSTSENTSCVTEEISAWSVYRTYNWNKSTQEMILFAFFVGYTTMMIPMGIIAQKFG 404
LC + N V I+ + + YNW+ +T +I +FF GY I GI A KFG
Sbjct: 137 LCFSSFLLCNMDRVNMSIAILPMSQEYNWSSATVGLIQSSFFWGYLLTQILGGIWADKFG 196
Query: 405 GKFPISVALFVNGVVSIISPWIPLFSGWMGLATCRLIQGMTQAALYPSMHTMLGKWAPLS 584
GK + + +I++P I G L R G+ + P+M+ ML KW P+S
Sbjct: 197 GKVVLGFGVVWWSFATIMTP-IAARLGLPFLLVVRAFMGIGEGVAMPAMNNMLSKWIPVS 255
Query: 585 ERGRLSTYVYTGSQFGTI 638
ER R VY+G G++
Sbjct: 256 ERSRSLALVYSGMYLGSV 273
>At5g44370.1 68418.m05433 transporter-related similar to vesicular
glutamate transporter 2 [Mus musculus] GI:15811369,
Na-dependent inorganic phosphate cotransporter [Homo
sapiens] GI:7328923; contains Pfam profile PF00083:
major facilitator superfamily protein
Length = 432
Score = 70.1 bits (164), Expect = 1e-12
Identities = 38/110 (34%), Positives = 59/110 (53%)
Frame = +3
Query: 312 NKSTQEMILFAFFVGYTTMMIPMGIIAQKFGGKFPISVALFVNGVVSIISPWIPLFSGWM 491
N+S++ IL FFVGY +P G AQK GG+ + ++ + + P P G +
Sbjct: 44 NQSSKGTILSTFFVGYACSQVPGGWAAQKIGGRKVLLLSFVLWSSTCFLVPLDPNRVGLL 103
Query: 492 GLATCRLIQGMTQAALYPSMHTMLGKWAPLSERGRLSTYVYTGSQFGTIL 641
+A RL+ G+ Q ++PS+HT+L +W P ER RL + +G G L
Sbjct: 104 VVA--RLLVGVAQGFIFPSIHTVLAQWVPPHERSRLVSITTSGMYLGAAL 151
>At2g29650.2 68415.m03604 inorganic phosphate transporter, putative
similar to brain specific Na+-dependent inorganic
phosphate cotransporter [Rattus norvegicus] GI:507415;
contains Pfam profile PF00083: major facilitator
superfamily protein
Length = 398
Score = 68.5 bits (160), Expect = 4e-12
Identities = 40/138 (28%), Positives = 62/138 (44%)
Frame = +3
Query: 225 LCSNVSTSENTSCVTEEISAWSVYRTYNWNKSTQEMILFAFFVGYTTMMIPMGIIAQKFG 404
LC + N V I+ + Y WN +T +I +FF GY I GI A G
Sbjct: 108 LCFSAFLLCNMDRVNMSIAILPMSAEYGWNPATVGLIQSSFFWGYLLTQIAGGIWADTVG 167
Query: 405 GKFPISVALFVNGVVSIISPWIPLFSGWMGLATCRLIQGMTQAALYPSMHTMLGKWAPLS 584
GK + + + +I++P + G L R G+ + P+M+ +L KW P+
Sbjct: 168 GKRVLGFGVIWWSIATILTP-VAAKLGLPYLLVVRAFMGVGEGVAMPAMNNILSKWVPVQ 226
Query: 585 ERGRLSTYVYTGSQFGTI 638
ER R VY+G G++
Sbjct: 227 ERSRSLALVYSGMYLGSV 244
>At2g29650.1 68415.m03603 inorganic phosphate transporter, putative
similar to brain specific Na+-dependent inorganic
phosphate cotransporter [Rattus norvegicus] GI:507415;
contains Pfam profile PF00083: major facilitator
superfamily protein
Length = 512
Score = 68.5 bits (160), Expect = 4e-12
Identities = 40/138 (28%), Positives = 62/138 (44%)
Frame = +3
Query: 225 LCSNVSTSENTSCVTEEISAWSVYRTYNWNKSTQEMILFAFFVGYTTMMIPMGIIAQKFG 404
LC + N V I+ + Y WN +T +I +FF GY I GI A G
Sbjct: 108 LCFSAFLLCNMDRVNMSIAILPMSAEYGWNPATVGLIQSSFFWGYLLTQIAGGIWADTVG 167
Query: 405 GKFPISVALFVNGVVSIISPWIPLFSGWMGLATCRLIQGMTQAALYPSMHTMLGKWAPLS 584
GK + + + +I++P + G L R G+ + P+M+ +L KW P+
Sbjct: 168 GKRVLGFGVIWWSIATILTP-VAAKLGLPYLLVVRAFMGVGEGVAMPAMNNILSKWVPVQ 226
Query: 585 ERGRLSTYVYTGSQFGTI 638
ER R VY+G G++
Sbjct: 227 ERSRSLALVYSGMYLGSV 244
>At2g29650.3 68415.m03602 inorganic phosphate transporter, putative
similar to brain specific Na+-dependent inorganic
phosphate cotransporter [Rattus norvegicus] GI:507415;
contains Pfam profile PF00083: major facilitator
superfamily protein
Length = 400
Score = 66.9 bits (156), Expect = 1e-11
Identities = 37/128 (28%), Positives = 59/128 (46%)
Frame = +3
Query: 255 TSCVTEEISAWSVYRTYNWNKSTQEMILFAFFVGYTTMMIPMGIIAQKFGGKFPISVALF 434
+ V I+ + Y WN +T +I +FF GY I GI A GGK + +
Sbjct: 6 SEAVNMSIAILPMSAEYGWNPATVGLIQSSFFWGYLLTQIAGGIWADTVGGKRVLGFGVI 65
Query: 435 VNGVVSIISPWIPLFSGWMGLATCRLIQGMTQAALYPSMHTMLGKWAPLSERGRLSTYVY 614
+ +I++P + G L R G+ + P+M+ +L KW P+ ER R VY
Sbjct: 66 WWSIATILTP-VAAKLGLPYLLVVRAFMGVGEGVAMPAMNNILSKWVPVQERSRSLALVY 124
Query: 615 TGSQFGTI 638
+G G++
Sbjct: 125 SGMYLGSV 132
>At3g46980.2 68416.m05102 transporter-related low similarity to
brain specific Na+-dependent inorganic phosphate
cotransporter from [Rattus norvegicus] GI:507415, [Homo
sapiens] GI:7328925, vesicular glutamate transporter 3
from [Rattus norvegicus] GI:21685382; contains Pfam
profile PF00083: major facilitator superfamily protein
Length = 469
Score = 60.1 bits (139), Expect = 1e-09
Identities = 29/111 (26%), Positives = 53/111 (47%)
Frame = +3
Query: 309 WNKSTQEMILFAFFVGYTTMMIPMGIIAQKFGGKFPISVALFVNGVVSIISPWIPLFSGW 488
W+KS ++ +F GY I G + ++GGK ++ + + + + ++PW S W
Sbjct: 145 WSKSFSGIVQSSFLWGYLISPIAGGTLVDRYGGKVVMAWGVALWSLATFLTPWAADSSLW 204
Query: 489 MGLATCRLIQGMTQAALYPSMHTMLGKWAPLSERGRLSTYVYTGSQFGTIL 641
L R + G+ + P M+ M+ +W P +ER R G Q G ++
Sbjct: 205 -ALLAARAMVGVAEGVALPCMNNMVARWFPPTERSRAVGIAMAGFQLGNVV 254
>At3g46980.1 68416.m05101 transporter-related low similarity to
brain specific Na+-dependent inorganic phosphate
cotransporter from [Rattus norvegicus] GI:507415, [Homo
sapiens] GI:7328925, vesicular glutamate transporter 3
from [Rattus norvegicus] GI:21685382; contains Pfam
profile PF00083: major facilitator superfamily protein
Length = 533
Score = 60.1 bits (139), Expect = 1e-09
Identities = 29/111 (26%), Positives = 53/111 (47%)
Frame = +3
Query: 309 WNKSTQEMILFAFFVGYTTMMIPMGIIAQKFGGKFPISVALFVNGVVSIISPWIPLFSGW 488
W+KS ++ +F GY I G + ++GGK ++ + + + + ++PW S W
Sbjct: 145 WSKSFSGIVQSSFLWGYLISPIAGGTLVDRYGGKVVMAWGVALWSLATFLTPWAADSSLW 204
Query: 489 MGLATCRLIQGMTQAALYPSMHTMLGKWAPLSERGRLSTYVYTGSQFGTIL 641
L R + G+ + P M+ M+ +W P +ER R G Q G ++
Sbjct: 205 -ALLAARAMVGVAEGVALPCMNNMVARWFPPTERSRAVGIAMAGFQLGNVV 254
>At5g20380.1 68418.m02424 transporter-related low similarity to
vesicular glutamate transporter 3 [Rattus norvegicus]
GI:21685382
Length = 517
Score = 57.2 bits (132), Expect = 1e-08
Identities = 34/145 (23%), Positives = 69/145 (47%), Gaps = 1/145 (0%)
Frame = +3
Query: 252 NTSCVTEEISAWSVYRTYNWNKSTQEMILFAFFVGYTTMMIPMGIIAQKFGGKFPISVAL 431
N V I+ + + W+ S ++ +FF GY +P G +++ FGG+ + + +
Sbjct: 113 NMDKVNLSIAIIPMSHQFGWSSSVAGLVQSSFFWGYALSQLPGGWLSKIFGGRKVLEIGV 172
Query: 432 FVNGVVSIISPWIPLFSGWM-GLATCRLIQGMTQAALYPSMHTMLGKWAPLSERGRLSTY 608
F S + +PL +G+M GL R++ G+ + + ++ + P+ ER R +
Sbjct: 173 F---TWSFATALVPLLAGFMPGLIFSRILVGIGEGVSPSAATDLIARTIPVKERSRAVGF 229
Query: 609 VYTGSQFGTILIFEISGLFAGTPVL 683
V+ G G+++ GL P++
Sbjct: 230 VFGGLSLGSVM-----GLLLAPPII 249
>At2g38060.1 68415.m04672 transporter-related low similarity to
vesicular glutamate transporter 3 [Homo sapiens]
GI:21213895, brain specific Na+-dependent inorganic
phosphate cotransporter [Rattus norvegicus] GI:507415;
contains Pfam profile PF00083: major facilitator
superfamily protein
Length = 512
Score = 53.2 bits (122), Expect = 2e-07
Identities = 26/111 (23%), Positives = 51/111 (45%)
Frame = +3
Query: 309 WNKSTQEMILFAFFVGYTTMMIPMGIIAQKFGGKFPISVALFVNGVVSIISPWIPLFSGW 488
W+ S ++ +F GY + G + ++GGK ++ + + + ++++PW S
Sbjct: 132 WSSSFLGVVQSSFLWGYIFSSVIGGALVDRYGGKRVLAWGVALWSLATLLTPWAAAHST- 190
Query: 489 MGLATCRLIQGMTQAALYPSMHTMLGKWAPLSERGRLSTYVYTGSQFGTIL 641
+ L R G+ + PSM T+L +W P+ ER G G ++
Sbjct: 191 LALLCVRAFFGLAEGVAMPSMTTLLSRWFPMDERASAVGISMAGFHMGNVV 241
>At3g13050.1 68416.m01626 transporter-related low similarity to
apical organic cation transporter [Sus scrofa]
GI:2062135, SP|Q02563 Synaptic vesicle protein 2 (SV2)
{Rattus norvegicus}; contains Pfam profile PF00083:
major facilitator superfamily protein
Length = 500
Score = 37.1 bits (82), Expect = 0.011
Identities = 35/135 (25%), Positives = 59/135 (43%), Gaps = 3/135 (2%)
Frame = +3
Query: 309 WNKSTQE--MILFAFFVGYTTMMIPMGIIAQKFGGKFPISVALFVNGVVSIISPWIPLFS 482
WN S ++ +I F G GI++ K G + + V V +S + P +
Sbjct: 55 WNLSARQESLITSVVFAGMLIGAYSWGIVSDKHGRRKGFIITAVVTFVAGFLSAFSPNYM 114
Query: 483 GWMGLATCRLIQGMTQAALYPSMHTMLGKWAPLSERGRLSTYVYTGSQFGTI-LIFEISG 659
W+ + C + G+ + S + ++ P RG T++ S F T+ IFE S
Sbjct: 115 -WLIILRCLVGLGLGGGPVLASWYL---EFIPAPSRG---TWMVVFSAFWTVGTIFEASL 167
Query: 660 LFAGTPVLGWXRYIL 704
+ P LGW R++L
Sbjct: 168 AWLVMPRLGW-RWLL 181
>At5g26800.1 68418.m03197 expressed protein predicted protein,
Arabidopsis thaliana
Length = 128
Score = 32.3 bits (70), Expect = 0.33
Identities = 18/52 (34%), Positives = 26/52 (50%), Gaps = 2/52 (3%)
Frame = -1
Query: 660 VLIFRRSRLFRIEIPCRHKCSIFLFHSTVPIYPALC--ACSDRELLVSSLES 511
+L+ R RL + IPC+H CS FL +T+ + C + V SL S
Sbjct: 56 LLVTRTLRLKKFGIPCKHVCSHFLLLTTIYFFHVAVNRFCEQYHVWVCSLNS 107
>At1g68900.1 68414.m07885 mandelate racemase/muconate lactonizing
enzyme C-terminal domain-containing protein / hydrolase,
alpha/beta fold family protein contains Pfam profiles
PF01188: Mandelate racemase / muconate lactonizing
enzyme, C-terminal domain, PF00561: hydrolase,
alpha/beta fold family
Length = 656
Score = 31.1 bits (67), Expect = 0.75
Identities = 13/29 (44%), Positives = 18/29 (62%)
Frame = +3
Query: 420 SVALFVNGVVSIISPWIPLFSGWMGLATC 506
SVALF++G + WIP+ +G G A C
Sbjct: 381 SVALFLHGFLGTGEEWIPIMTGISGSARC 409
>At1g75710.1 68414.m08795 zinc finger (C2H2 type) family protein
contains zinc finger, C2H2 type, domain, PROSITE:PS00028
Length = 462
Score = 29.9 bits (64), Expect = 1.7
Identities = 16/48 (33%), Positives = 26/48 (54%)
Frame = -1
Query: 645 RSRLFRIEIPCRHKCSIFLFHSTVPIYPALCACSDRELLVSSLESIDM 502
R+ FR C ++C + + S PI P +CACS + LES+++
Sbjct: 175 RAMQFRKLSGC-YECHMIVDPSRYPISPRVCACSQCGEVFPKLESLEL 221
>At4g28080.1 68417.m04027 expressed protein
Length = 1660
Score = 27.9 bits (59), Expect = 7.0
Identities = 19/66 (28%), Positives = 27/66 (40%), Gaps = 2/66 (3%)
Frame = +3
Query: 114 HTQALVLFICLTVAYTMRAQLSVSMVAITGHAVTESELCSNV--STSENTSCVTEEISAW 287
H Q+L + + AY Q V+ V T T C NV T +T V +E W
Sbjct: 599 HVQSLCVHEMIVRAYKHILQAVVAAVENTADVATSIATCLNVLLGTPSDTESVYDEKIKW 658
Query: 288 SVYRTY 305
+ T+
Sbjct: 659 TWVETF 664
>At3g19610.1 68416.m02486 hypothetical protein
Length = 612
Score = 27.9 bits (59), Expect = 7.0
Identities = 11/31 (35%), Positives = 19/31 (61%)
Frame = +1
Query: 34 STRGYQRMTNMKGHHQKFRHQKALAPATLKL 126
S +QR T++ HHQ+ +H++ L T+ L
Sbjct: 582 SLLSHQRSTSLMSHHQQQQHEQHLFQKTMSL 612
>At3g49840.1 68416.m05449 proline-rich family protein contains
proline rich extensin domains, INTERPRO:IPR002965
Length = 606
Score = 27.5 bits (58), Expect = 9.3
Identities = 15/48 (31%), Positives = 23/48 (47%)
Frame = +3
Query: 312 NKSTQEMILFAFFVGYTTMMIPMGIIAQKFGGKFPISVALFVNGVVSI 455
N ST E++ F Y + + MGI+ + SVA + G +SI
Sbjct: 153 NVSTVELVAKQEFFIYRIVFLIMGIVLLSLASRLSKSVAFYYIGAMSI 200
>At2g13100.1 68415.m01436 glycerol-3-phosphate transporter, putative
/ glycerol 3-phosphate permease, putative similar to
glycerol-3-phosphate transporter (glycerol 3-phosphate
permease) [Homo sapiens] GI:7543982; contains Pfam
profile PF00083: major facilitator superfamily protein
Length = 493
Score = 27.5 bits (58), Expect = 9.3
Identities = 21/124 (16%), Positives = 56/124 (45%), Gaps = 3/124 (2%)
Frame = +3
Query: 279 SAWSVYRTYNWNKSTQEMILFAFFVGYTTMMIPMGIIAQKFGGKFPISVALFVNGVVSII 458
+ W+ + K E+ L AF Y M G + + ++ + + +G+++++
Sbjct: 69 NGWAPFNGTQGTKRLGELDL-AFLSSYALGMYFAGHLGDRIDLRYFLVFGMMGSGILTLV 127
Query: 459 ---SPWIPLFSGWMGLATCRLIQGMTQAALYPSMHTMLGKWAPLSERGRLSTYVYTGSQF 629
W+ + + ++ +++ G+ Q+ +P + +++G W +RG + + +
Sbjct: 128 FGLGYWMNVHTLGFYMSV-QIVCGLFQSIGWPCVVSVVGNWCGKEKRGLIMGLWNSHTSV 186
Query: 630 GTIL 641
G IL
Sbjct: 187 GNIL 190
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,673,410
Number of Sequences: 28952
Number of extensions: 365254
Number of successful extensions: 902
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 879
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 896
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1535986264
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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