BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= bmmt10a10 (701 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC4F8.10c |stg1||SM22/transgelin-like actin modulating protein... 79 5e-16 SPAC4F8.13c |rng2||IQGAP|Schizosaccharomyces pombe|chr 1|||Manual 55 1e-08 SPBC1778.06c |fim1||fimbrin|Schizosaccharomyces pombe|chr 2|||Ma... 38 0.001 SPBC17D1.07c |||GTPase regulator |Schizosaccharomyces pombe|chr ... 36 0.007 SPCC1919.05 |||TPR repeat protein Ski3 |Schizosaccharomyces pomb... 30 0.28 SPAC15A10.08 |ain1||alpha-actinin|Schizosaccharomyces pombe|chr ... 30 0.37 SPBC839.16 |||C-1-tetrahydrofolate synthase|Schizosaccharomyces ... 30 0.37 SPAC17G8.02 |||uridine ribohydrolase |Schizosaccharomyces pombe|... 27 2.0 SPAC20G4.02c |fus1||formin Fus1|Schizosaccharomyces pombe|chr 1|... 27 2.0 SPAC29A4.11 |rga3||GTPase activating protein Rga3|Schizosaccharo... 27 2.6 SPBC14C8.01c |cut2|SPBC1815.02c|securin|Schizosaccharomyces pomb... 27 3.4 SPAC1296.04 |mug65||spore wall assembly protein |Schizosaccharom... 27 3.4 SPCPB16A4.03c |ade10||IMP cyclohydrolase|Schizosaccharomyces pom... 26 4.5 SPAC1F3.05 |||adaptin |Schizosaccharomyces pombe|chr 1|||Manual 26 6.0 >SPAC4F8.10c |stg1||SM22/transgelin-like actin modulating protein Stg1|Schizosaccharomyces pombe|chr 1|||Manual Length = 174 Score = 79.4 bits (187), Expect = 5e-16 Identities = 58/168 (34%), Positives = 90/168 (53%), Gaps = 8/168 (4%) Frame = +1 Query: 133 EIAQETLEWIRKLTGEPANTSGDADNLYEVLKDGTLLCKLVNELQPGSVKKINQTTMAFK 312 ++ +E EWI + N D L + L+ G +LC++ E +++ ++ M F Sbjct: 4 QLEKEAREWIEETLHTKLNAQLD---LLDQLQSGVILCRICKEALGANIR-YKESNMPFV 59 Query: 313 CMENINAFLE-AVKKLGVPAQETFQTIDLWERQNLYSVVVCLQSLGRKAGNF--GK-PSI 480 MENI+AF+ A + + VP+Q+ FQT DL+ER+N V+ + S R A GK + Sbjct: 60 QMENISAFINYAQQVVHVPSQDMFQTSDLFERRNDEQVLRSIHSFSRYAAKMFPGKVRGL 119 Query: 481 GPKEADKNVRDFS-EEQLKAGQNVISLQYGTNKGQQSG---ISFGNRR 612 GPK A+K R FS ++Q + + V SLQYG+ G I+F RR Sbjct: 120 GPKLAEKKPRVFSAQQQREFREGVNSLQYGSFDMPTQGTEKIAFSRRR 167 >SPAC4F8.13c |rng2||IQGAP|Schizosaccharomyces pombe|chr 1|||Manual Length = 1489 Score = 54.8 bits (126), Expect = 1e-08 Identities = 30/101 (29%), Positives = 53/101 (52%), Gaps = 1/101 (0%) Frame = +1 Query: 145 ETLEWIRKLTGEPANTSGDADNLYEVLKDGTLLCKLVNELQPGSVKKINQTT-MAFKCME 321 E +WI + G G + L++G +L LV + QP + KI + + F+ + Sbjct: 46 EAKKWIEECLGTDL---GPTSTFEQSLRNGVVLALLVQKFQPDKLIKIFYSNELQFRHSD 102 Query: 322 NINAFLEAVKKLGVPAQETFQTIDLWERQNLYSVVVCLQSL 444 NIN FL+ + +G+P F+ D++E +NL V+ C+ +L Sbjct: 103 NINKFLDFIHGIGLPEIFHFELTDIYEGKNLPKVIYCIHAL 143 >SPBC1778.06c |fim1||fimbrin|Schizosaccharomyces pombe|chr 2|||Manual Length = 614 Score = 38.3 bits (85), Expect = 0.001 Identities = 28/112 (25%), Positives = 54/112 (48%), Gaps = 13/112 (11%) Frame = +1 Query: 64 EYRAGKAGINAEAQARIHSKYNDEIAQETLEWIRK-LTGEP-----ANTSGDADNLYEVL 225 E + G+ I + + H+ N+E +E ++ I L G+P + + ++ Sbjct: 91 EVKKGRITIKGSSSSVSHT-INEEERREFIKHINSVLAGDPDVGSRVPINTETFEFFDQC 149 Query: 226 KDGTLLCKLVNELQPGSV-------KKINQTTMAFKCMENINAFLEAVKKLG 360 KDG +L KL+N+ P ++ ++ N+ FKC+EN N + + K +G Sbjct: 150 KDGLILSKLINDSVPDTIDERVLNKQRNNKPLDNFKCIENNNVVINSAKAMG 201 >SPBC17D1.07c |||GTPase regulator |Schizosaccharomyces pombe|chr 2|||Manual Length = 962 Score = 35.5 bits (78), Expect = 0.007 Identities = 25/101 (24%), Positives = 47/101 (46%), Gaps = 1/101 (0%) Frame = +1 Query: 145 ETLEWIRKLTGEPANTSGDADNLYEVLKDGTLLCKLVNELQPGSVKKIN-QTTMAFKCME 321 E +W+ + T N + D+ + L +G +LC+L + P + ++ + Sbjct: 69 EAKKWLEEETN---NEYQNLDDFVDALVNGKVLCQLAFKYYPKLASNWKPRYQISERNTV 125 Query: 322 NINAFLEAVKKLGVPAQETFQTIDLWERQNLYSVVVCLQSL 444 +NAF + +G+ F+T DL R N+ V+ CL +L Sbjct: 126 YLNAFFHFLDFIGMFTPFRFETKDLVRRFNIPKVIYCLHAL 166 >SPCC1919.05 |||TPR repeat protein Ski3 |Schizosaccharomyces pombe|chr 3|||Manual Length = 1389 Score = 30.3 bits (65), Expect = 0.28 Identities = 21/63 (33%), Positives = 30/63 (47%) Frame = +1 Query: 208 NLYEVLKDGTLLCKLVNELQPGSVKKINQTTMAFKCMENINAFLEAVKKLGVPAQETFQT 387 NLYE D + L K+ P K + KC+ +N ++E VKK G A E F+ Sbjct: 78 NLYESTHDISELHKIT----PILASKFLELEEQNKCLNTVNKYMEVVKKYGNKADE-FKA 132 Query: 388 IDL 396 + L Sbjct: 133 LKL 135 >SPAC15A10.08 |ain1||alpha-actinin|Schizosaccharomyces pombe|chr 1|||Manual Length = 621 Score = 29.9 bits (64), Expect = 0.37 Identities = 17/54 (31%), Positives = 30/54 (55%), Gaps = 1/54 (1%) Frame = +1 Query: 208 NLYEVLKDGTLLCKLVNELQPGSVKKINQTT-MAFKCMENINAFLEAVKKLGVP 366 +L + L DG LL +L+ + ++ + N+ M +EN+N LE +K G+P Sbjct: 32 DLRKDLSDGILLIQLLEIIGDENLGRYNRNPRMRVHRLENVNKALEYIKSKGMP 85 >SPBC839.16 |||C-1-tetrahydrofolate synthase|Schizosaccharomyces pombe|chr 2|||Manual Length = 937 Score = 29.9 bits (64), Expect = 0.37 Identities = 17/52 (32%), Positives = 26/52 (50%) Frame = -1 Query: 224 KTSYRLSASPEVLAGSPVNFLIHSRVSWAISSLYLLWILAWASALMPAFPAR 69 KT Y LS P+ L G+P NF + R + ++ LA A + +P P + Sbjct: 869 KTQYSLSHDPD-LKGAPTNFTVPIRDMRLSAGAGFIYPLAAAISTIPGLPTK 919 >SPAC17G8.02 |||uridine ribohydrolase |Schizosaccharomyces pombe|chr 1|||Manual Length = 330 Score = 27.5 bits (58), Expect = 2.0 Identities = 33/127 (25%), Positives = 51/127 (40%), Gaps = 1/127 (0%) Frame = +1 Query: 67 YRAGKAGI-NAEAQARIHSKYNDEIAQETLEWIRKLTGEPANTSGDADNLYEVLKDGTLL 243 + G GI N +QA + + E A+ LE + L G+ D ++VL D ++ Sbjct: 163 FMGGSTGIGNITSQAEFNVYADPEAARLVLE-TKSLIGKLFMVPLDVT--HKVLLDANII 219 Query: 244 CKLVNELQPGSVKKINQTTMAFKCMENINAFLEAVKKLGVPAQETFQTIDLWERQNLYSV 423 L P S + T+ + EN+ V V A LW +++Y V Sbjct: 220 QLLRQHSNPFSSTLVELMTVFQQTYENVYGIRNGVPVHDVCAVALALWPSLWTSRSMY-V 278 Query: 424 VVCLQSL 444 V L SL Sbjct: 279 TVSLDSL 285 >SPAC20G4.02c |fus1||formin Fus1|Schizosaccharomyces pombe|chr 1|||Manual Length = 1372 Score = 27.5 bits (58), Expect = 2.0 Identities = 23/90 (25%), Positives = 37/90 (41%), Gaps = 4/90 (4%) Frame = +1 Query: 151 LEWIRKLTGEPANTSGDADNLYEVLKDGTLLCKLVNELQPGSVKKINQTTMAFKCMEN-- 324 L+ +R + G+ T N Y + D + C L +L V+K+ + F C Sbjct: 246 LKSMRCIIGQKIGTDFYLSNKYPI--DSVVHCLLSTKLL---VRKLAAELLTFLCYSEKP 300 Query: 325 --INAFLEAVKKLGVPAQETFQTIDLWERQ 408 INA ++ +K E+ DLW Q Sbjct: 301 NGINAIMKCMKNFANLKVESMNVFDLWLSQ 330 >SPAC29A4.11 |rga3||GTPase activating protein Rga3|Schizosaccharomyces pombe|chr 1|||Manual Length = 969 Score = 27.1 bits (57), Expect = 2.6 Identities = 11/59 (18%), Positives = 29/59 (49%) Frame = +1 Query: 199 DADNLYEVLKDGTLLCKLVNELQPGSVKKINQTTMAFKCMENINAFLEAVKKLGVPAQE 375 + + +Y + + + LV+E + GS++ + T+ F C + +L + + +P + Sbjct: 810 EVEGIYRISGSASQVRVLVDEFENGSIRMEHLTSDLFACTSVLKTYLHRLPEPVIPGTQ 868 >SPBC14C8.01c |cut2|SPBC1815.02c|securin|Schizosaccharomyces pombe|chr 2|||Manual Length = 301 Score = 26.6 bits (56), Expect = 3.4 Identities = 13/26 (50%), Positives = 15/26 (57%) Frame = -2 Query: 565 RTVAISRSDRLSAAPRRSRAHSCPPP 488 R+ RS RLSA+PRRS P P Sbjct: 96 RSAVQERSKRLSASPRRSSLTDTPLP 121 >SPAC1296.04 |mug65||spore wall assembly protein |Schizosaccharomyces pombe|chr 1|||Manual Length = 248 Score = 26.6 bits (56), Expect = 3.4 Identities = 14/51 (27%), Positives = 23/51 (45%) Frame = -2 Query: 529 AAPRRSRAHSCPPPWARWTACRSYLPSYRGTVDKRPPSKGSASPKDQWSGR 377 A+P ++ +CP P W R Y+ G VD+ G + + W G+ Sbjct: 48 ASPVSTKTATCPDPSWEWAWQRWYI-DMSGDVDESGWQYGFSFTMNNWHGK 97 >SPCPB16A4.03c |ade10||IMP cyclohydrolase|Schizosaccharomyces pombe|chr 3|||Manual Length = 585 Score = 26.2 bits (55), Expect = 4.5 Identities = 11/35 (31%), Positives = 19/35 (54%) Frame = +1 Query: 496 DKNVRDFSEEQLKAGQNVISLQYGTNKGQQSGISF 600 D + D+ +Q AG + ++L+YG N Q +F Sbjct: 179 DAAITDYFRKQYAAGVDQLTLRYGANPHQSPAQAF 213 >SPAC1F3.05 |||adaptin |Schizosaccharomyces pombe|chr 1|||Manual Length = 510 Score = 25.8 bits (54), Expect = 6.0 Identities = 18/82 (21%), Positives = 35/82 (42%) Frame = +1 Query: 103 QARIHSKYNDEIAQETLEWIRKLTGEPANTSGDADNLYEVLKDGTLLCKLVNELQPGSVK 282 ++ + ND + +L + KLT EPA S + ++L L N + S Sbjct: 317 ESHVPPSSNDTKQESSLIDLMKLTEEPAVPSPSLPTNVPANQSLSMLSSLSNSMSSTSNG 376 Query: 283 KINQTTMAFKCMENINAFLEAV 348 +N + + + N N+ L ++ Sbjct: 377 ALNSPSYSQAAIPNTNSSLTSI 398 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,887,195 Number of Sequences: 5004 Number of extensions: 60710 Number of successful extensions: 233 Number of sequences better than 10.0: 14 Number of HSP's better than 10.0 without gapping: 224 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 231 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 325165428 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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