BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= bmmt10a01 (650 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g21105.1 68418.m02515 L-ascorbate oxidase, putative similar t... 29 2.7 At5g15630.1 68418.m01829 phytochelatin synthetase family protein... 28 4.7 >At5g21105.1 68418.m02515 L-ascorbate oxidase, putative similar to L-ascorbate oxidase from {Nicotiana tabacum} SP|Q40588, {Cucurbita pepo var. melopepo} SP|P37064; contains Pfam profile PF00394: Multicopper oxidase; supported by cDNA gi_15215753_gb_AY050406.1_; A false intron was added between exons 4 and 5 to circumvent the single nucleotide insertion in this BAC which, otherwise, causes a frameshift. Length = 571 Score = 29.1 bits (62), Expect = 2.7 Identities = 13/23 (56%), Positives = 15/23 (65%) Frame = -2 Query: 352 KSRPRSYFLWYDAIMPPVLNNTT 284 KS PRSY + YD + PP NTT Sbjct: 410 KSPPRSYRMDYDIMNPPPFPNTT 432 >At5g15630.1 68418.m01829 phytochelatin synthetase family protein / COBRA cell expansion protein COBL4 similar to phytochelatin synthetase [Hordeum vulgare subsp. vulgare] GI:29570314; identified in Roudier, et al, Plant Phys. (2002) 130:538-548 (PMID:12376623); contains Pfam profile PF04833: Phytochelatin synthetase-like conserved region Length = 431 Score = 28.3 bits (60), Expect = 4.7 Identities = 13/32 (40%), Positives = 19/32 (59%) Frame = -2 Query: 337 SYFLWYDAIMPPVLNNTTAIIPIEFKPNISRY 242 ++ LW AI P LNN T + ++KP +S Y Sbjct: 306 NHTLWTLAIQHPNLNNVTQVFSFDYKP-VSPY 336 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 13,545,712 Number of Sequences: 28952 Number of extensions: 266511 Number of successful extensions: 410 Number of sequences better than 10.0: 2 Number of HSP's better than 10.0 without gapping: 407 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 410 length of database: 12,070,560 effective HSP length: 78 effective length of database: 9,812,304 effective search space used: 1354097952 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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