Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= an--0970
(762 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g67280.1 68414.m07657 lactoylglutathione lyase, putative / gl... 82 3e-16
At1g11840.2 68414.m01362 lactoylglutathione lyase, putative / gl... 76 3e-14
At1g11840.1 68414.m01361 lactoylglutathione lyase, putative / gl... 76 3e-14
At1g11840.3 68414.m01360 lactoylglutathione lyase, putative / gl... 75 5e-14
At1g08110.2 68414.m00889 lactoylglutathione lyase, putative / gl... 32 0.48
At1g08110.1 68414.m00888 lactoylglutathione lyase, putative / gl... 32 0.48
At2g26680.1 68415.m03200 expressed protein similar to NLPE1 (GI:... 29 3.4
At4g23090.1 68417.m03328 hypothetical protein 28 7.8
>At1g67280.1 68414.m07657 lactoylglutathione lyase, putative /
glyoxalase I, putative similar to putative
lactoylglutathione lyase SP:Q39366, GI:2494843 from
[Brassica oleracea]
Length = 350
Score = 82.2 bits (194), Expect = 3e-16
Identities = 48/131 (36%), Positives = 69/131 (52%), Gaps = 11/131 (8%)
Frame = +2
Query: 158 RALHFVFKVADRTLTAKFYREILGMKVLRHEEFSEGCEAACNGPYANRWSKTMVGYGPED 337
R LH V++V D T KFY E LGMK+LR + E +++ +GYGPED
Sbjct: 88 RMLHVVYRVGDMDRTIKFYTECLGMKLLRKRDIPE-----------EKYTNAFLGYGPED 136
Query: 338 THFVVELTYNYGVTHYEQGNDF--LGITVQS-SESLKRAQTNNWPIKEHNG--------L 484
+HFV+ELTYNYGV Y+ G F GI V +++++ + + G +
Sbjct: 137 SHFVIELTYNYGVDKYDIGAGFGHFGIAVDDVAKTVELVKAKGGKVSREPGPVKGGKTVI 196
Query: 485 KYVEAPGGYKF 517
++E P GYKF
Sbjct: 197 AFIEDPDGYKF 207
Score = 62.5 bits (145), Expect = 3e-10
Identities = 34/92 (36%), Positives = 52/92 (56%)
Frame = +2
Query: 173 VFKVADRTLTAKFYREILGMKVLRHEEFSEGCEAACNGPYANRWSKTMVGYGPEDTHFVV 352
+ +V D KFY + GM++LR + N Y +++ M+GYGPED V+
Sbjct: 223 MLRVGDLDRAIKFYEKAFGMELLRTRD---------NPEY--KYTIAMMGYGPEDKFPVL 271
Query: 353 ELTYNYGVTHYEQGNDFLGITVQSSESLKRAQ 448
ELTYNYGVT Y++GN + I + + + K A+
Sbjct: 272 ELTYNYGVTEYDKGNAYAQIAIGTDDVYKTAE 303
>At1g11840.2 68414.m01362 lactoylglutathione lyase, putative /
glyoxalase I, putative highly similar to putative
lactoylglutathione lyase SP:Q39366 from [Brassica
oleracea]
Length = 283
Score = 75.8 bits (178), Expect = 3e-14
Identities = 65/219 (29%), Positives = 103/219 (47%), Gaps = 22/219 (10%)
Frame = +2
Query: 158 RALHFVFKVADRTLTAKFYREILGMKVLRHEEFSEGCEAACNGPYANRWSKTMVGYGPED 337
R LH V++V D T +FY E+ GMK+LR + E ++S +G+GPE
Sbjct: 17 RFLHVVYRVGDLDRTIEFYTEVFGMKLLRKRDIPE-----------EKYSNAFLGFGPET 65
Query: 338 THFVVELTYNYGVTHYEQGNDF--LGITVQSSESL---KRAQTNN-----WPIKEHNG-L 484
++FVVELTYNYGV+ Y+ G F I+ Q L RA+ N P+K +
Sbjct: 66 SNFVVELTYNYGVSSYDIGTGFGHFAISTQDVSKLVENVRAKGGNVTREPGPVKGGGSVI 125
Query: 485 KYVEAPGGYKFYIXXXXXXXXXXXXXXXSLASSNLAKSIAYWNGLLTLKLYEKTDK---- 652
+V+ P GY F + L +L ++I ++ L ++L K ++
Sbjct: 126 AFVKDPDGYTFEL--IQRGPTPEPFCQVMLRVGDLDRAIKFYEKALGMRLLRKIERPEYK 183
Query: 653 --TALLGYSDDQAK--LELV---DIGEPINRAKAYGRIA 748
++GY+++ LEL D+ E + AY +IA
Sbjct: 184 YTIGMMGYAEEYESIVLELTYNYDVTE-YTKGNAYAQIA 221
Score = 50.0 bits (114), Expect = 2e-06
Identities = 30/92 (32%), Positives = 46/92 (50%)
Frame = +2
Query: 173 VFKVADRTLTAKFYREILGMKVLRHEEFSEGCEAACNGPYANRWSKTMVGYGPEDTHFVV 352
+ +V D KFY + LGM++LR E E +++ M+GY E V+
Sbjct: 152 MLRVGDLDRAIKFYEKALGMRLLRKIERPE-----------YKYTIGMMGYAEEYESIVL 200
Query: 353 ELTYNYGVTHYEQGNDFLGITVQSSESLKRAQ 448
ELTYNY VT Y +GN + I + + + K +
Sbjct: 201 ELTYNYDVTEYTKGNAYAQIAIGTDDVYKSGE 232
>At1g11840.1 68414.m01361 lactoylglutathione lyase, putative /
glyoxalase I, putative highly similar to putative
lactoylglutathione lyase SP:Q39366 from [Brassica
oleracea]
Length = 283
Score = 75.8 bits (178), Expect = 3e-14
Identities = 65/219 (29%), Positives = 103/219 (47%), Gaps = 22/219 (10%)
Frame = +2
Query: 158 RALHFVFKVADRTLTAKFYREILGMKVLRHEEFSEGCEAACNGPYANRWSKTMVGYGPED 337
R LH V++V D T +FY E+ GMK+LR + E ++S +G+GPE
Sbjct: 17 RFLHVVYRVGDLDRTIEFYTEVFGMKLLRKRDIPE-----------EKYSNAFLGFGPET 65
Query: 338 THFVVELTYNYGVTHYEQGNDF--LGITVQSSESL---KRAQTNN-----WPIKEHNG-L 484
++FVVELTYNYGV+ Y+ G F I+ Q L RA+ N P+K +
Sbjct: 66 SNFVVELTYNYGVSSYDIGTGFGHFAISTQDVSKLVENVRAKGGNVTREPGPVKGGGSVI 125
Query: 485 KYVEAPGGYKFYIXXXXXXXXXXXXXXXSLASSNLAKSIAYWNGLLTLKLYEKTDK---- 652
+V+ P GY F + L +L ++I ++ L ++L K ++
Sbjct: 126 AFVKDPDGYTFEL--IQRGPTPEPFCQVMLRVGDLDRAIKFYEKALGMRLLRKIERPEYK 183
Query: 653 --TALLGYSDDQAK--LELV---DIGEPINRAKAYGRIA 748
++GY+++ LEL D+ E + AY +IA
Sbjct: 184 YTIGMMGYAEEYESIVLELTYNYDVTE-YTKGNAYAQIA 221
Score = 50.0 bits (114), Expect = 2e-06
Identities = 30/92 (32%), Positives = 46/92 (50%)
Frame = +2
Query: 173 VFKVADRTLTAKFYREILGMKVLRHEEFSEGCEAACNGPYANRWSKTMVGYGPEDTHFVV 352
+ +V D KFY + LGM++LR E E +++ M+GY E V+
Sbjct: 152 MLRVGDLDRAIKFYEKALGMRLLRKIERPE-----------YKYTIGMMGYAEEYESIVL 200
Query: 353 ELTYNYGVTHYEQGNDFLGITVQSSESLKRAQ 448
ELTYNY VT Y +GN + I + + + K +
Sbjct: 201 ELTYNYDVTEYTKGNAYAQIAIGTDDVYKSGE 232
>At1g11840.3 68414.m01360 lactoylglutathione lyase, putative /
glyoxalase I, putative highly similar to putative
lactoylglutathione lyase SP:Q39366 from [Brassica
oleracea]
Length = 232
Score = 74.9 bits (176), Expect = 5e-14
Identities = 64/222 (28%), Positives = 102/222 (45%), Gaps = 22/222 (9%)
Frame = +2
Query: 158 RALHFVFKVADRTLTAKFYREILGMKVLRHEEFSEGCEAACNGPYANRWSKTMVGYGPED 337
R LH V++V D T +FY E+ GMK+LR + E ++S +G+GPE
Sbjct: 17 RFLHVVYRVGDLDRTIEFYTEVFGMKLLRKRDIPE-----------EKYSNAFLGFGPET 65
Query: 338 THFVVELTYNYGVTHYEQGNDF--LGITVQSSESL---KRAQTNN-----WPIKEHNG-L 484
++FVVELTYNYGV+ Y+ G F I+ Q L RA+ N P+K +
Sbjct: 66 SNFVVELTYNYGVSSYDIGTGFGHFAISTQDVSKLVENVRAKGGNVTREPGPVKGGGSVI 125
Query: 485 KYVEAPGGYKFYIXXXXXXXXXXXXXXXSLASSNLAKSIAYWNGLLTLKLYEKTDK---- 652
+V+ P GY F + L +L ++I ++ L ++L K ++
Sbjct: 126 AFVKDPDGYTFEL--IQRGPTPEPFCQVMLRVGDLDRAIKFYEKALGMRLLRKIERPEYK 183
Query: 653 --TALLGYSDDQAK--LELV---DIGEPINRAKAYGRIAFSC 757
++GY+++ LEL D+ E + AY + C
Sbjct: 184 YTIGMMGYAEEYESIVLELTYNYDVTE-YTKGNAYAQAQMMC 224
Score = 48.4 bits (110), Expect = 5e-06
Identities = 29/89 (32%), Positives = 46/89 (51%)
Frame = +2
Query: 173 VFKVADRTLTAKFYREILGMKVLRHEEFSEGCEAACNGPYANRWSKTMVGYGPEDTHFVV 352
+ +V D KFY + LGM++LR E E +++ M+GY E V+
Sbjct: 152 MLRVGDLDRAIKFYEKALGMRLLRKIERPE-----------YKYTIGMMGYAEEYESIVL 200
Query: 353 ELTYNYGVTHYEQGNDFLGITVQSSESLK 439
ELTYNY VT Y +GN + + ++++K
Sbjct: 201 ELTYNYDVTEYTKGNAYAQAQMMCTKAVK 229
>At1g08110.2 68414.m00889 lactoylglutathione lyase, putative /
glyoxalase I, putative similar to lactoylglutathione
lyase SP:O04885 from [Brassica juncea]
Length = 185
Score = 31.9 bits (69), Expect = 0.48
Identities = 27/94 (28%), Positives = 44/94 (46%), Gaps = 8/94 (8%)
Frame = +2
Query: 173 VFKVADRTLTAKFYREILGMKVLRHEEFSE--------GCEAACNGPYANRWSKTMVGYG 328
+F++ D + FY +LGM +L+ +FSE G E P + +T+ +G
Sbjct: 32 MFRIKDPKASLDFYSRVLGMSLLKRLDFSEMKFSLYFLGYEDTTTAP-TDPTERTVWTFG 90
Query: 329 PEDTHFVVELTYNYGVTHYEQGNDFLGITVQSSE 430
T +ELT+N+G E +F G +SE
Sbjct: 91 QPAT---IELTHNWGT---ESDPEFKGYHNGNSE 118
>At1g08110.1 68414.m00888 lactoylglutathione lyase, putative /
glyoxalase I, putative similar to lactoylglutathione
lyase SP:O04885 from [Brassica juncea]
Length = 185
Score = 31.9 bits (69), Expect = 0.48
Identities = 27/94 (28%), Positives = 44/94 (46%), Gaps = 8/94 (8%)
Frame = +2
Query: 173 VFKVADRTLTAKFYREILGMKVLRHEEFSE--------GCEAACNGPYANRWSKTMVGYG 328
+F++ D + FY +LGM +L+ +FSE G E P + +T+ +G
Sbjct: 32 MFRIKDPKASLDFYSRVLGMSLLKRLDFSEMKFSLYFLGYEDTTTAP-TDPTERTVWTFG 90
Query: 329 PEDTHFVVELTYNYGVTHYEQGNDFLGITVQSSE 430
T +ELT+N+G E +F G +SE
Sbjct: 91 QPAT---IELTHNWGT---ESDPEFKGYHNGNSE 118
>At2g26680.1 68415.m03200 expressed protein similar to NLPE1
(GI:13022100) [Rhizobium etli];
Length = 319
Score = 29.1 bits (62), Expect = 3.4
Identities = 18/58 (31%), Positives = 27/58 (46%)
Frame = +2
Query: 128 KLLNSKMVSGRALHFVFKVADRTLTAKFYREILGMKVLRHEEFSEGCEAACNGPYANR 301
++L+S SG+ V V A F ++G KVL E E + C+G + NR
Sbjct: 121 EVLDSMRASGKN-GIVVDVGANVGMASFAAAVMGFKVLAFEPVFENLQRICDGIWFNR 177
>At4g23090.1 68417.m03328 hypothetical protein
Length = 179
Score = 27.9 bits (59), Expect = 7.8
Identities = 16/38 (42%), Positives = 20/38 (52%), Gaps = 1/38 (2%)
Frame = -2
Query: 341 VYLQGHIQPWFCSIGLHKVHCKLPR-ILH*TLHGGEPS 231
+YL G QP +G+ VH P ILH +L G PS
Sbjct: 114 LYLSGWFQPMLTDVGV-AVHSSRPNGILHVSLSGVSPS 150
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,738,678
Number of Sequences: 28952
Number of extensions: 282925
Number of successful extensions: 756
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 732
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 748
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1702303248
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -