Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= an--0961
(768 letters)
Database: uniref50
1,657,284 sequences; 575,637,011 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
UniRef50_UPI0000E4A7B0 Cluster: PREDICTED: similar to NifU-like ... 76 8e-13
UniRef50_Q9H1K1 Cluster: Iron-sulfur cluster assembly enzyme ISC... 75 1e-12
UniRef50_Q6CFQ0 Cluster: Iron sulfur cluster assembly protein 1,... 73 8e-12
UniRef50_A2QHV4 Cluster: Remark: alternate names for S. cerevisi... 69 2e-10
UniRef50_Q4WNS3 Cluster: Iron-sulfur cofactor synthesis protein ... 67 4e-10
UniRef50_Q9UTC6 Cluster: Iron sulfur cluster assembly protein 1,... 67 5e-10
UniRef50_A2ZW89 Cluster: Putative uncharacterized protein; n=1; ... 65 2e-09
UniRef50_Q6E0I7 Cluster: Iron-sulfur cluster assembly protein; n... 64 3e-09
UniRef50_Q0CL81 Cluster: Iron sulfur cluster assembly protein 1,... 62 2e-08
UniRef50_Q4N9C2 Cluster: NifU protein, putative; n=11; cellular ... 60 8e-08
UniRef50_Q7QTV3 Cluster: GLP_76_35055_35693; n=4; Eukaryota|Rep:... 59 1e-07
UniRef50_Q38DM2 Cluster: Iron-sulfur cluster assembly protein, p... 56 1e-06
UniRef50_UPI00001D2CFF Cluster: UPI00001D2CFF related cluster; n... 47 6e-04
UniRef50_Q896F6 Cluster: NifU protein; n=7; cellular organisms|R... 46 0.001
UniRef50_Q8TME1 Cluster: NifU protein; n=24; cellular organisms|... 46 0.001
UniRef50_P05340 Cluster: Nitrogen fixation protein nifU; n=17; B... 44 0.004
UniRef50_O42770 Cluster: Putative uncharacterized protein; n=1; ... 41 0.029
UniRef50_P20628 Cluster: Nitrogen fixation protein nifU; n=24; B... 41 0.029
UniRef50_Q8KB04 Cluster: IscU protein; n=10; Chlorobiaceae|Rep: ... 39 0.16
UniRef50_P05343 Cluster: Nitrogen fixation protein nifU; n=4; Pr... 38 0.27
UniRef50_Q8TSI9 Cluster: NifU family protein; n=5; Methanomicrob... 37 0.63
UniRef50_Q2LWE9 Cluster: NifU-like protein involved in Fe-S clus... 36 0.84
UniRef50_A4LX66 Cluster: Fe-S cluster assembly protein NifU; n=2... 36 1.1
UniRef50_O34393 Cluster: NifU protein; n=24; cellular organisms|... 36 1.5
UniRef50_Q3A8K8 Cluster: NifU-like protein; n=3; Desulfuromonada... 35 1.9
UniRef50_Q2RIL0 Cluster: Nitrogen-fixing NifU-like; n=3; Clostri... 35 1.9
UniRef50_O67045 Cluster: NifU-like protein; n=4; cellular organi... 35 1.9
UniRef50_A7H4A3 Cluster: Peptidase, M48 family; n=12; Campylobac... 34 3.4
UniRef50_A6DAA5 Cluster: NifU family protein; n=2; Caminibacter ... 34 4.5
>UniRef50_UPI0000E4A7B0 Cluster: PREDICTED: similar to NifU-like
protein; n=2; Strongylocentrotus purpuratus|Rep:
PREDICTED: similar to NifU-like protein -
Strongylocentrotus purpuratus
Length = 163
Score = 76.2 bits (179), Expect = 8e-13
Identities = 34/41 (82%), Positives = 38/41 (92%)
Frame = -3
Query: 760 LKNTDIAKELSLPPVKLHCSMLAEDAIKAALSDYRINQQTE 638
+KNTDIAKELSLPPVKLHCSMLAEDAIKAAL DYR+ Q ++
Sbjct: 118 IKNTDIAKELSLPPVKLHCSMLAEDAIKAALQDYRVKQDSK 158
>UniRef50_Q9H1K1 Cluster: Iron-sulfur cluster assembly enzyme ISCU,
mitochondrial precursor; n=320; cellular organisms|Rep:
Iron-sulfur cluster assembly enzyme ISCU, mitochondrial
precursor - Homo sapiens (Human)
Length = 167
Score = 75.4 bits (177), Expect = 1e-12
Identities = 34/46 (73%), Positives = 40/46 (86%)
Frame = -3
Query: 766 LKLKNTDIAKELSLPPVKLHCSMLAEDAIKAALSDYRINQQTENKE 629
L +KNTDIAKEL LPPVKLHCSMLAEDAIKAAL+DY++ Q+ + E
Sbjct: 118 LTIKNTDIAKELCLPPVKLHCSMLAEDAIKAALADYKLKQEPKKGE 163
>UniRef50_Q6CFQ0 Cluster: Iron sulfur cluster assembly protein 1,
mitochondrial precursor; n=5; Ascomycota|Rep: Iron
sulfur cluster assembly protein 1, mitochondrial
precursor - Yarrowia lipolytica (Candida lipolytica)
Length = 181
Score = 72.9 bits (171), Expect = 8e-12
Identities = 33/43 (76%), Positives = 39/43 (90%)
Frame = -3
Query: 763 KLKNTDIAKELSLPPVKLHCSMLAEDAIKAALSDYRINQQTEN 635
K+KNT IAKELSLPPVKLHCSMLAEDAIK+A+SDY ++T+N
Sbjct: 121 KIKNTVIAKELSLPPVKLHCSMLAEDAIKSAISDYNSKRKTKN 163
>UniRef50_A2QHV4 Cluster: Remark: alternate names for S. cerevisiae
Isu2: Nua2; n=8; cellular organisms|Rep: Remark:
alternate names for S. cerevisiae Isu2: Nua2 -
Aspergillus niger
Length = 198
Score = 68.5 bits (160), Expect = 2e-10
Identities = 30/35 (85%), Positives = 34/35 (97%)
Frame = -3
Query: 763 KLKNTDIAKELSLPPVKLHCSMLAEDAIKAALSDY 659
K++NTDIAKEL LPPVKLHCSMLAEDAIK+A+SDY
Sbjct: 130 KIRNTDIAKELCLPPVKLHCSMLAEDAIKSAISDY 164
>UniRef50_Q4WNS3 Cluster: Iron-sulfur cofactor synthesis protein
(Isu1), putative; n=2; Eurotiomycetidae|Rep: Iron-sulfur
cofactor synthesis protein (Isu1), putative -
Aspergillus fumigatus (Sartorya fumigata)
Length = 255
Score = 67.3 bits (157), Expect = 4e-10
Identities = 29/35 (82%), Positives = 34/35 (97%)
Frame = -3
Query: 763 KLKNTDIAKELSLPPVKLHCSMLAEDAIKAALSDY 659
K++NTDIAKEL LPPVKLHCSMLAEDAIK+A++DY
Sbjct: 189 KIRNTDIAKELCLPPVKLHCSMLAEDAIKSAINDY 223
>UniRef50_Q9UTC6 Cluster: Iron sulfur cluster assembly protein 1,
mitochondrial precursor; n=6; Dikarya|Rep: Iron sulfur
cluster assembly protein 1, mitochondrial precursor -
Schizosaccharomyces pombe (Fission yeast)
Length = 192
Score = 66.9 bits (156), Expect = 5e-10
Identities = 31/41 (75%), Positives = 34/41 (82%)
Frame = -3
Query: 763 KLKNTDIAKELSLPPVKLHCSMLAEDAIKAALSDYRINQQT 641
K+KNT IAKEL LPPVKLHCSMLAEDAIK+A+ YR Q T
Sbjct: 138 KIKNTQIAKELCLPPVKLHCSMLAEDAIKSAVKHYRSKQLT 178
>UniRef50_A2ZW89 Cluster: Putative uncharacterized protein; n=1;
Oryza sativa (japonica cultivar-group)|Rep: Putative
uncharacterized protein - Oryza sativa subsp. japonica
(Rice)
Length = 156
Score = 65.3 bits (152), Expect = 2e-09
Identities = 29/34 (85%), Positives = 32/34 (94%)
Frame = -3
Query: 760 LKNTDIAKELSLPPVKLHCSMLAEDAIKAALSDY 659
+KNT+IAK LSLPPVKLHCSMLAEDAIKAA+ DY
Sbjct: 105 IKNTEIAKHLSLPPVKLHCSMLAEDAIKAAVKDY 138
>UniRef50_Q6E0I7 Cluster: Iron-sulfur cluster assembly protein; n=1;
Toxoplasma gondii|Rep: Iron-sulfur cluster assembly
protein - Toxoplasma gondii
Length = 219
Score = 64.5 bits (150), Expect = 3e-09
Identities = 28/42 (66%), Positives = 37/42 (88%)
Frame = -3
Query: 766 LKLKNTDIAKELSLPPVKLHCSMLAEDAIKAALSDYRINQQT 641
LKLKNTDIA+ L+LPPVK+HCS+LAEDA++ A+ DY+ Q+T
Sbjct: 152 LKLKNTDIAEYLNLPPVKVHCSLLAEDAVRHAILDYQKKQKT 193
>UniRef50_Q0CL81 Cluster: Iron sulfur cluster assembly protein 1,
mitochondrial; n=1; Aspergillus terreus NIH2624|Rep:
Iron sulfur cluster assembly protein 1, mitochondrial -
Aspergillus terreus (strain NIH 2624)
Length = 179
Score = 61.7 bits (143), Expect = 2e-08
Identities = 28/35 (80%), Positives = 31/35 (88%)
Frame = -3
Query: 763 KLKNTDIAKELSLPPVKLHCSMLAEDAIKAALSDY 659
K+ N IAKEL LPPVKLHCSMLAEDAIK+A+SDY
Sbjct: 108 KVTNVAIAKELCLPPVKLHCSMLAEDAIKSAISDY 142
>UniRef50_Q4N9C2 Cluster: NifU protein, putative; n=11; cellular
organisms|Rep: NifU protein, putative - Theileria parva
Length = 301
Score = 59.7 bits (138), Expect = 8e-08
Identities = 24/36 (66%), Positives = 33/36 (91%)
Frame = -3
Query: 766 LKLKNTDIAKELSLPPVKLHCSMLAEDAIKAALSDY 659
L +KNTDI+ +L+LPPVK+HCS+LAEDA+K A++DY
Sbjct: 260 LAIKNTDISDKLNLPPVKIHCSLLAEDAVKHAINDY 295
>UniRef50_Q7QTV3 Cluster: GLP_76_35055_35693; n=4; Eukaryota|Rep:
GLP_76_35055_35693 - Giardia lamblia ATCC 50803
Length = 212
Score = 58.8 bits (136), Expect = 1e-07
Identities = 24/36 (66%), Positives = 32/36 (88%)
Frame = -3
Query: 763 KLKNTDIAKELSLPPVKLHCSMLAEDAIKAALSDYR 656
++KNTDI+ +L LPPVKLHCS+LAEDAI+ A+ DY+
Sbjct: 164 QIKNTDISDKLGLPPVKLHCSVLAEDAIRQAIDDYK 199
>UniRef50_Q38DM2 Cluster: Iron-sulfur cluster assembly protein,
putative; n=2; Trypanosoma|Rep: Iron-sulfur cluster
assembly protein, putative - Trypanosoma brucei
Length = 180
Score = 55.6 bits (128), Expect = 1e-06
Identities = 25/36 (69%), Positives = 31/36 (86%)
Frame = -3
Query: 766 LKLKNTDIAKELSLPPVKLHCSMLAEDAIKAALSDY 659
L+L N IA+ELSLPPVKLHCSMLAE+ I+AA+ +Y
Sbjct: 106 LQLTNKRIARELSLPPVKLHCSMLAEETIQAAVENY 141
>UniRef50_UPI00001D2CFF Cluster: UPI00001D2CFF related cluster; n=1;
unknown|Rep: UPI00001D2CFF UniRef100 entry - unknown
Length = 51
Score = 46.8 bits (106), Expect = 6e-04
Identities = 19/29 (65%), Positives = 26/29 (89%)
Frame = -3
Query: 733 LSLPPVKLHCSMLAEDAIKAALSDYRINQ 647
LSLPPVK+HCS+ AEDA++AA++DY+ Q
Sbjct: 15 LSLPPVKIHCSVPAEDAMRAAVADYQAEQ 43
>UniRef50_Q896F6 Cluster: NifU protein; n=7; cellular organisms|Rep:
NifU protein - Clostridium tetani
Length = 158
Score = 45.6 bits (103), Expect = 0.001
Identities = 21/37 (56%), Positives = 28/37 (75%), Gaps = 1/37 (2%)
Frame = -3
Query: 763 KLKNTDIAKELS-LPPVKLHCSMLAEDAIKAALSDYR 656
+L N +A L LPPVK+HCS+LAE+AI A++DYR
Sbjct: 99 ELSNKAVADALDGLPPVKMHCSVLAEEAIHKAINDYR 135
>UniRef50_Q8TME1 Cluster: NifU protein; n=24; cellular
organisms|Rep: NifU protein - Methanosarcina acetivorans
Length = 128
Score = 45.6 bits (103), Expect = 0.001
Identities = 20/36 (55%), Positives = 28/36 (77%), Gaps = 1/36 (2%)
Frame = -3
Query: 763 KLKNTDIAKELS-LPPVKLHCSMLAEDAIKAALSDY 659
+L N +A+ L LPP+K+HCSMLAE+AI A++DY
Sbjct: 83 ELSNKAVAEALDGLPPIKMHCSMLAEEAIHEAINDY 118
>UniRef50_P05340 Cluster: Nitrogen fixation protein nifU; n=17;
Bacteria|Rep: Nitrogen fixation protein nifU -
Azotobacter vinelandii
Length = 312
Score = 44.0 bits (99), Expect = 0.004
Identities = 19/38 (50%), Positives = 29/38 (76%), Gaps = 1/38 (2%)
Frame = -3
Query: 766 LKLKNTDIAKELS-LPPVKLHCSMLAEDAIKAALSDYR 656
LK+ N DIA L LPP K+HCS++ +A++AA+++YR
Sbjct: 85 LKISNQDIADYLDGLPPEKMHCSVMGREALQAAVANYR 122
>UniRef50_O42770 Cluster: Putative uncharacterized protein; n=1;
Paxillus involutus|Rep: Putative uncharacterized protein
- Paxillus involutus (Naked brimcap)
Length = 47
Score = 41.1 bits (92), Expect = 0.029
Identities = 17/21 (80%), Positives = 20/21 (95%)
Frame = -3
Query: 718 VKLHCSMLAEDAIKAALSDYR 656
VKLHCSMLAEDAI++A+ DYR
Sbjct: 1 VKLHCSMLAEDAIRSAIRDYR 21
>UniRef50_P20628 Cluster: Nitrogen fixation protein nifU; n=24;
Bacteria|Rep: Nitrogen fixation protein nifU - Anabaena
sp. (strain PCC 7120)
Length = 300
Score = 41.1 bits (92), Expect = 0.029
Identities = 18/38 (47%), Positives = 28/38 (73%), Gaps = 1/38 (2%)
Frame = -3
Query: 766 LKLKNTDIAKELS-LPPVKLHCSMLAEDAIKAALSDYR 656
LK+ N DIA L LP K+HCS++ ++A++AA+ +YR
Sbjct: 91 LKVSNKDIADYLGGLPEAKMHCSVMGQEALEAAIYNYR 128
>UniRef50_Q8KB04 Cluster: IscU protein; n=10; Chlorobiaceae|Rep:
IscU protein - Chlorobium tepidum
Length = 203
Score = 38.7 bits (86), Expect = 0.16
Identities = 17/33 (51%), Positives = 26/33 (78%), Gaps = 1/33 (3%)
Frame = -3
Query: 748 DIAKELS-LPPVKLHCSMLAEDAIKAALSDYRI 653
++AKEL LP K+HCS+L + A++AA++DY I
Sbjct: 99 EVAKELGGLPENKIHCSVLGDKALRAAINDYFI 131
>UniRef50_P05343 Cluster: Nitrogen fixation protein nifU; n=4;
Proteobacteria|Rep: Nitrogen fixation protein nifU -
Klebsiella pneumoniae
Length = 274
Score = 37.9 bits (84), Expect = 0.27
Identities = 15/37 (40%), Positives = 28/37 (75%), Gaps = 1/37 (2%)
Frame = -3
Query: 763 KLKNTDIAKELS-LPPVKLHCSMLAEDAIKAALSDYR 656
++ N IA L LPP K+HCS++ ++A++AA++++R
Sbjct: 86 QITNQQIADYLDGLPPEKMHCSVMGQEALRAAIANFR 122
>UniRef50_Q8TSI9 Cluster: NifU family protein; n=5;
Methanomicrobia|Rep: NifU family protein -
Methanosarcina acetivorans
Length = 220
Score = 36.7 bits (81), Expect = 0.63
Identities = 13/23 (56%), Positives = 19/23 (82%)
Frame = -3
Query: 727 LPPVKLHCSMLAEDAIKAALSDY 659
LPP+K HCS+LA + ++AA+ DY
Sbjct: 109 LPPIKAHCSVLAVEGLRAAIRDY 131
>UniRef50_Q2LWE9 Cluster: NifU-like protein involved in Fe-S cluster
formation with 2Fe-2S domain; n=1; Syntrophus
aciditrophicus SB|Rep: NifU-like protein involved in
Fe-S cluster formation with 2Fe-2S domain - Syntrophus
aciditrophicus (strain SB)
Length = 229
Score = 36.3 bits (80), Expect = 0.84
Identities = 17/37 (45%), Positives = 24/37 (64%), Gaps = 1/37 (2%)
Frame = -3
Query: 763 KLKNTDIAKELS-LPPVKLHCSMLAEDAIKAALSDYR 656
K+ N DIA L LP K+HCS+L E ++ A++ YR
Sbjct: 85 KITNQDIAAALGGLPEQKMHCSVLGEQVLEKAIACYR 121
>UniRef50_A4LX66 Cluster: Fe-S cluster assembly protein NifU; n=20;
cellular organisms|Rep: Fe-S cluster assembly protein
NifU - Geobacter bemidjiensis Bem
Length = 284
Score = 35.9 bits (79), Expect = 1.1
Identities = 15/38 (39%), Positives = 27/38 (71%), Gaps = 1/38 (2%)
Frame = -3
Query: 766 LKLKNTDIAKELS-LPPVKLHCSMLAEDAIKAALSDYR 656
L++ N +IA L LP K+HCS++ ++A++ A++ YR
Sbjct: 85 LQISNQEIADFLGGLPEEKMHCSVMGQEALEVAIAKYR 122
>UniRef50_O34393 Cluster: NifU protein; n=24; cellular
organisms|Rep: NifU protein - Archaeoglobus fulgidus
Length = 153
Score = 35.5 bits (78), Expect = 1.5
Identities = 17/37 (45%), Positives = 24/37 (64%), Gaps = 1/37 (2%)
Frame = -3
Query: 766 LKLKNTDIAKELS-LPPVKLHCSMLAEDAIKAALSDY 659
LK+ +A+ L LP K+HCS LA DA++ A+ DY
Sbjct: 81 LKITRDAVAEALGGLPKQKMHCSNLAADALRRAIVDY 117
>UniRef50_Q3A8K8 Cluster: NifU-like protein; n=3;
Desulfuromonadales|Rep: NifU-like protein - Pelobacter
carbinolicus (strain DSM 2380 / Gra Bd 1)
Length = 143
Score = 35.1 bits (77), Expect = 1.9
Identities = 18/37 (48%), Positives = 24/37 (64%), Gaps = 1/37 (2%)
Frame = -3
Query: 766 LKLKNTDIAKELS-LPPVKLHCSMLAEDAIKAALSDY 659
++L ++A L LPP K+HCS LA A+KAAL Y
Sbjct: 81 MELTEKEVAVALGGLPPQKMHCSNLAVGALKAALRVY 117
>UniRef50_Q2RIL0 Cluster: Nitrogen-fixing NifU-like; n=3;
Clostridia|Rep: Nitrogen-fixing NifU-like - Moorella
thermoacetica (strain ATCC 39073)
Length = 139
Score = 35.1 bits (77), Expect = 1.9
Identities = 16/47 (34%), Positives = 27/47 (57%), Gaps = 1/47 (2%)
Frame = -3
Query: 766 LKLKNTDIAKELS-LPPVKLHCSMLAEDAIKAALSDYRINQQTENKE 629
L++ + D+A + LP VKLHCS L A+ A+ +Y+ Q ++
Sbjct: 81 LRITDADVAAAIGGLPEVKLHCSNLGASALHDAIRNYQAKMQAGGQQ 127
>UniRef50_O67045 Cluster: NifU-like protein; n=4; cellular
organisms|Rep: NifU-like protein - Aquifex aeolicus
Length = 157
Score = 35.1 bits (77), Expect = 1.9
Identities = 17/41 (41%), Positives = 24/41 (58%), Gaps = 1/41 (2%)
Frame = -3
Query: 766 LKLKNTDIAKELS-LPPVKLHCSMLAEDAIKAALSDYRINQ 647
L L DI +EL LPP K+HC+ L + + A+ DY + Q
Sbjct: 86 LNLTYKDIFEELGGLPPQKIHCTNLGLETLHVAIKDYLMKQ 126
>UniRef50_A7H4A3 Cluster: Peptidase, M48 family; n=12;
Campylobacter|Rep: Peptidase, M48 family - Campylobacter
jejuni subsp. doylei 269.97
Length = 395
Score = 34.3 bits (75), Expect = 3.4
Identities = 16/43 (37%), Positives = 24/43 (55%)
Frame = +3
Query: 90 EVIYPTTFLTYYNNSTFQILHLLTNIFLWLLDKINNCKNRKNQ 218
E +Y + L N F +L +L NIF +L+ I N +RKN+
Sbjct: 298 EFVYLESHLEGVNGGVFALLFILANIFSFLISPILNALSRKNE 340
>UniRef50_A6DAA5 Cluster: NifU family protein; n=2; Caminibacter
mediatlanticus TB-2|Rep: NifU family protein -
Caminibacter mediatlanticus TB-2
Length = 321
Score = 33.9 bits (74), Expect = 4.5
Identities = 18/53 (33%), Positives = 28/53 (52%), Gaps = 7/53 (13%)
Frame = -3
Query: 766 LKLKNTDIAKEL-------SLPPVKLHCSMLAEDAIKAALSDYRINQQTENKE 629
+K+ N D+ K L + PP K+HCS++A D I A + Y+ E K+
Sbjct: 104 MKVTNLDVEKALRDEPNKPAFPPQKMHCSVMAYDVIMEAAAKYKGKDAEELKD 156
Database: uniref50
Posted date: Oct 5, 2007 11:19 AM
Number of letters in database: 575,637,011
Number of sequences in database: 1,657,284
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 615,834,274
Number of Sequences: 1657284
Number of extensions: 11187914
Number of successful extensions: 21903
Number of sequences better than 10.0: 29
Number of HSP's better than 10.0 without gapping: 21022
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 21899
length of database: 575,637,011
effective HSP length: 99
effective length of database: 411,565,895
effective search space used: 64204279620
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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