Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= an--0959
(380 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g15020.1 68416.m01900 malate dehydrogenase [NAD], mitochondri... 83 5e-17
At1g53240.1 68414.m06033 malate dehydrogenase [NAD], mitochondri... 81 3e-16
At3g47520.1 68416.m05168 malate dehydrogenase [NAD], chloroplast... 79 1e-15
At2g22780.1 68415.m02702 malate dehydrogenase, glyoxysomal, puta... 71 3e-13
At5g09660.1 68418.m01117 malate dehydrogenase, glyoxysomal ident... 65 1e-11
At4g21030.1 68417.m03042 Dof-type zinc finger domain-containing ... 29 1.4
At2g36960.2 68415.m04533 myb family transcription factor contain... 27 4.2
At2g36960.1 68415.m04532 myb family transcription factor contain... 27 4.2
At1g45130.1 68414.m05173 beta-galactosidase, putative / lactase,... 27 4.2
At3g27400.1 68416.m03425 pectate lyase family protein similar to... 27 5.6
At2g47980.1 68415.m06004 expressed protein 26 7.4
At2g21140.1 68415.m02508 hydroxyproline-rich glycoprotein family... 26 7.4
At5g67170.2 68418.m08468 SEC-C motif-containing protein / OTU-li... 26 9.7
At5g67170.1 68418.m08467 SEC-C motif-containing protein / OTU-li... 26 9.7
At3g14860.2 68416.m01879 NHL repeat-containing protein contains ... 26 9.7
At3g14860.1 68416.m01878 NHL repeat-containing protein contains ... 26 9.7
At3g12000.1 68416.m01486 S-locus related protein SLR1, putative ... 26 9.7
At1g24625.1 68414.m03099 zinc finger (C2H2 type) family protein ... 26 9.7
At1g10320.1 68414.m01162 U2 snRNP auxiliary factor-related simil... 26 9.7
>At3g15020.1 68416.m01900 malate dehydrogenase [NAD], mitochondrial,
putative similar to mitochondrial NAD-dependent malate
dehydrogenase GB:CAA10320 SP|Q9ZP06 [Arabidopsis
thaliana]; contains InterPro entry IPR001236:
Lactate/malate dehydrogenase
Length = 341
Score = 83.4 bits (197), Expect = 5e-17
Identities = 46/104 (44%), Positives = 68/104 (65%), Gaps = 2/104 (1%)
Frame = +2
Query: 62 MFSRALKPAALAVQNGA--KNFSTTSQRNFKXXXXXXXXXIGQPLALLLKQNPLVTRLAL 235
MF + +A V+ G + F++ S + K IGQPL+LL+K NPLV+ L+L
Sbjct: 1 MFRSMIVRSASPVKQGLLRRGFASESVPDRKVVILGAAGGIGQPLSLLMKLNPLVSSLSL 60
Query: 236 YDIAPVTPGVAADLSHMNTPAKVSGHNGPEELSAAIKDADVVVI 367
YDIA TPGVAAD+ H+NT ++VSG+ G ++L A++ AD+V+I
Sbjct: 61 YDIAN-TPGVAADVGHINTRSQVSGYMGDDDLGKALEGADLVII 103
>At1g53240.1 68414.m06033 malate dehydrogenase [NAD], mitochondrial
identical to mitochondrial NAD-dependent malate
dehydrogenase GI:3929649 SP|Q9ZP06 from [Arabidopsis
thaliana]; contains InterPro entry IPR001236:
Lactate/malate dehydrogenase
Length = 341
Score = 80.6 bits (190), Expect = 3e-16
Identities = 42/85 (49%), Positives = 59/85 (69%)
Frame = +2
Query: 113 KNFSTTSQRNFKXXXXXXXXXIGQPLALLLKQNPLVTRLALYDIAPVTPGVAADLSHMNT 292
++FS+ S K IGQPLALL+K NPLV+ L+LYDIA TPGVAAD+ H+NT
Sbjct: 20 RSFSSGSVPERKVAILGAAGGIGQPLALLMKLNPLVSSLSLYDIAN-TPGVAADVGHINT 78
Query: 293 PAKVSGHNGPEELSAAIKDADVVVI 367
++V G+ G + L+ A++ AD+V+I
Sbjct: 79 RSEVVGYMGDDNLAKALEGADLVII 103
>At3g47520.1 68416.m05168 malate dehydrogenase [NAD], chloroplast
(MDH) identical to chloroplast NAD-malate dehydrogenase
[Arabidopsis thaliana] GI:3256066; contains InterPro
entry IPR001236: Lactate/malate dehydrogenase; contains
Pfam profiles PF00056: lactate/malate dehydrogenase, NAD
binding domain and PF02866: lactate/malate
dehydrogenase, alpha/beta C-terminal domain
Length = 403
Score = 79.0 bits (186), Expect = 1e-15
Identities = 40/64 (62%), Positives = 49/64 (76%)
Frame = +2
Query: 176 IGQPLALLLKQNPLVTRLALYDIAPVTPGVAADLSHMNTPAKVSGHNGPEELSAAIKDAD 355
IGQPL+LL+K +PLV+ L LYDIA V GVAADLSH NTP++V GP EL+ +KD +
Sbjct: 94 IGQPLSLLIKMSPLVSTLHLYDIANVK-GVAADLSHCNTPSQVRDFTGPSELADCLKDVN 152
Query: 356 VVVI 367
VVVI
Sbjct: 153 VVVI 156
>At2g22780.1 68415.m02702 malate dehydrogenase, glyoxysomal,
putative strong similarity to glyoxysomal malate
dehydrogenase (EC 1.1.1.37) SP|P19446 {Citrullus
lanatus}, SP|P46488 {Cucumis sativus}, [Medicago sativa]
GI:2827078, SP|Q42972 {Oryza sativa}, SP|Q9ZP05
{Arabidopsis thaliana}, SP|P37228 {Glycine max};
contains InterPro entry IPR001236: Lactate/malate
dehydrogenase
Length = 354
Score = 70.5 bits (165), Expect = 3e-13
Identities = 37/75 (49%), Positives = 47/75 (62%)
Frame = +2
Query: 143 FKXXXXXXXXXIGQPLALLLKQNPLVTRLALYDIAPVTPGVAADLSHMNTPAKVSGHNGP 322
FK IGQPLA+L+K NPLV+ L LYD+A PGV AD+SHM+T A V G G
Sbjct: 43 FKVAILGAAGGIGQPLAMLMKMNPLVSVLHLYDVAN-APGVTADISHMDTSAVVRGFLGQ 101
Query: 323 EELSAAIKDADVVVI 367
+L A+ D+V+I
Sbjct: 102 PQLEEALTGMDLVII 116
>At5g09660.1 68418.m01117 malate dehydrogenase, glyoxysomal
identical to SP|Q9ZP05; identical to cDNA microbody
NAD-dependent malate dehydrogenase GI:3929650
Length = 354
Score = 65.3 bits (152), Expect = 1e-11
Identities = 35/75 (46%), Positives = 46/75 (61%)
Frame = +2
Query: 143 FKXXXXXXXXXIGQPLALLLKQNPLVTRLALYDIAPVTPGVAADLSHMNTPAKVSGHNGP 322
FK IGQ L+LL+K NPLV+ L LYD+ PGV AD+SHM+T A V G G
Sbjct: 43 FKVAILGAAGGIGQSLSLLMKMNPLVSLLHLYDVVNA-PGVTADVSHMDTGAVVRGFLGA 101
Query: 323 EELSAAIKDADVVVI 367
++L A+ D+V+I
Sbjct: 102 KQLEDALTGMDLVII 116
>At4g21030.1 68417.m03042 Dof-type zinc finger domain-containing
protein prolamin box binding factor, Zea mays,
PID:g2393775
Length = 194
Score = 28.7 bits (61), Expect = 1.4
Identities = 10/25 (40%), Positives = 16/25 (64%)
Frame = +1
Query: 22 SNIHVYTR*TNNENVLPRPKTCRPC 96
+N++V+T N NV+P P+ C C
Sbjct: 2 NNLNVFTNEDNEMNVMPPPRVCPRC 26
>At2g36960.2 68415.m04533 myb family transcription factor contains
Pfam profile: PF00249 myb-like DNA-binding domain
Length = 741
Score = 27.1 bits (57), Expect = 4.2
Identities = 15/41 (36%), Positives = 18/41 (43%), Gaps = 1/41 (2%)
Frame = +1
Query: 193 PSTEAE-SSGDQAGFIRHSACDPRRRSRPFPHEHPSQGQRP 312
PS E SSGD+ + H DP PH S G+ P
Sbjct: 635 PSQLVEASSGDEGSYNPHDDGDPMEEGPADPHTMDSPGKTP 675
>At2g36960.1 68415.m04532 myb family transcription factor contains
Pfam profile: PF00249 myb-like DNA-binding domain
Length = 743
Score = 27.1 bits (57), Expect = 4.2
Identities = 15/41 (36%), Positives = 18/41 (43%), Gaps = 1/41 (2%)
Frame = +1
Query: 193 PSTEAE-SSGDQAGFIRHSACDPRRRSRPFPHEHPSQGQRP 312
PS E SSGD+ + H DP PH S G+ P
Sbjct: 637 PSQLVEASSGDEGSYNPHDDGDPMEEGPADPHTMDSPGKTP 677
>At1g45130.1 68414.m05173 beta-galactosidase, putative / lactase,
putative similar to beta-galactosidase [Lycopersicon
esculentum] GI:7939619, beta-galactosidase BG1
GI:15081596 from [Vitis vinifera]; contains Pfam profile
PF01301: Glycosyl hydrolases family 35
Length = 732
Score = 27.1 bits (57), Expect = 4.2
Identities = 21/53 (39%), Positives = 29/53 (54%), Gaps = 6/53 (11%)
Frame = +2
Query: 176 IGQPLALLLKQNPLVTRLALYDIAPV-TPGVAADLS-----HMNTPAKVSGHN 316
I Q A L+ +P VT+L Y+ A V T G + ++ HMN PAKV +N
Sbjct: 343 IKQCEAALVSSDPHVTKLGNYEEAHVFTAGKGSCVAFLTNYHMNAPAKVVFNN 395
>At3g27400.1 68416.m03425 pectate lyase family protein similar to
pectate lyase GP:7547009 from [Vitis vinifera]; contains
Pfam profile: PF00544 pectate lyase
Length = 412
Score = 26.6 bits (56), Expect = 5.6
Identities = 13/32 (40%), Positives = 17/32 (53%)
Frame = +1
Query: 10 GGNSSNIHVYTR*TNNENVLPRPKTCRPCCTK 105
GG I+V T N++ V PRP T R T+
Sbjct: 96 GGRKGRIYVVTDPANDDPVNPRPGTLRYAVTQ 127
>At2g47980.1 68415.m06004 expressed protein
Length = 1098
Score = 26.2 bits (55), Expect = 7.4
Identities = 12/33 (36%), Positives = 17/33 (51%)
Frame = +2
Query: 212 PLVTRLALYDIAPVTPGVAADLSHMNTPAKVSG 310
P TRL++ DI + V + H+NT SG
Sbjct: 969 PFATRLSVSDIIDIKKDVQGRIVHVNTDEDPSG 1001
>At2g21140.1 68415.m02508 hydroxyproline-rich glycoprotein family
protein identical to proline-rich protein 2 [Arabidopsis
thaliana] gi|7620011|gb|AAF64549
Length = 321
Score = 26.2 bits (55), Expect = 7.4
Identities = 9/23 (39%), Positives = 15/23 (65%)
Frame = +3
Query: 69 PAP*NLPPLLYKMVQRTFPPHHR 137
P P NLPPL + +++ PP ++
Sbjct: 167 PPPLNLPPLTFPKIKKPCPPIYK 189
>At5g67170.2 68418.m08468 SEC-C motif-containing protein / OTU-like
cysteine protease family protein contains Pfam profiles
PF02338: OTU-like cysteine protease, PF02810: SEC-C
motif
Length = 374
Score = 25.8 bits (54), Expect = 9.7
Identities = 12/33 (36%), Positives = 17/33 (51%)
Frame = +1
Query: 7 SGGNSSNIHVYTR*TNNENVLPRPKTCRPCCTK 105
SG NS + ++ +PR KTC PC +K
Sbjct: 290 SGNNSETVQAKCTTQTDDKKIPRNKTC-PCGSK 321
>At5g67170.1 68418.m08467 SEC-C motif-containing protein / OTU-like
cysteine protease family protein contains Pfam profiles
PF02338: OTU-like cysteine protease, PF02810: SEC-C
motif
Length = 375
Score = 25.8 bits (54), Expect = 9.7
Identities = 12/33 (36%), Positives = 17/33 (51%)
Frame = +1
Query: 7 SGGNSSNIHVYTR*TNNENVLPRPKTCRPCCTK 105
SG NS + ++ +PR KTC PC +K
Sbjct: 291 SGNNSETVQAKCTTQTDDKKIPRNKTC-PCGSK 322
>At3g14860.2 68416.m01879 NHL repeat-containing protein contains
Pfam profile PF01436: NHL repeat
Length = 493
Score = 25.8 bits (54), Expect = 9.7
Identities = 17/39 (43%), Positives = 19/39 (48%), Gaps = 3/39 (7%)
Frame = -1
Query: 191 PKAGRYHRRHQRPPL*SSSVM---WWKSSLHHFVQQGRQ 84
PKA + H+ P L SSSVM SS HH RQ
Sbjct: 384 PKADDSYPEHKTPKLRSSSVMKDPTLSSSKHHRTSSKRQ 422
>At3g14860.1 68416.m01878 NHL repeat-containing protein contains
Pfam profile PF01436: NHL repeat
Length = 492
Score = 25.8 bits (54), Expect = 9.7
Identities = 17/39 (43%), Positives = 19/39 (48%), Gaps = 3/39 (7%)
Frame = -1
Query: 191 PKAGRYHRRHQRPPL*SSSVM---WWKSSLHHFVQQGRQ 84
PKA + H+ P L SSSVM SS HH RQ
Sbjct: 383 PKADDSYPEHKTPKLRSSSVMKDPTLSSSKHHRTSSKRQ 421
>At3g12000.1 68416.m01486 S-locus related protein SLR1, putative
(S1) identical to S-locus related protein SLR1 homolog
(AtS1) GI:246209 Arabidopsis thaliana]; contains Pfam
profiles PF01453: Lectin (probable mannose binding),
PF00954: S-locus glycoprotein family
Length = 439
Score = 25.8 bits (54), Expect = 9.7
Identities = 10/24 (41%), Positives = 16/24 (66%)
Frame = -1
Query: 299 WLGCSCGKGRLRRRGSQALCRIKP 228
W G + G LRRR S+++C+ +P
Sbjct: 415 WTGGAHGYEELRRRRSRSICQNRP 438
>At1g24625.1 68414.m03099 zinc finger (C2H2 type) family protein
(ZFP7) identical to zinc finger protein, ZFP7
gi|790685|gb|AAA87303; contains Pfam domain, PF00096:
Zinc finger, C2H2 type
Length = 209
Score = 25.8 bits (54), Expect = 9.7
Identities = 21/75 (28%), Positives = 33/75 (44%), Gaps = 3/75 (4%)
Frame = -1
Query: 287 SCGKGRLRRRGSQALCRIKPAWSPEDSASVEGPKAGRYHRRHQRPPL*SSSVMWWKSS-- 114
SC R + SQAL + A E + + GR H RP +SS + ++
Sbjct: 60 SCNYCRRKFYSSQALGGHQNAHKRERTMAKRAMHMGRMFGHHHRPYTYTSSSLGMQAHSG 119
Query: 113 -LHHFVQQGRQVLGR 72
LHH + Q + ++ R
Sbjct: 120 LLHHTLSQPQPLVSR 134
>At1g10320.1 68414.m01162 U2 snRNP auxiliary factor-related similar
to U2 small nuclear ribonucleoprotein auxiliary factor
35 kD subunit related protein 1 (sp|Q15695)
Length = 757
Score = 25.8 bits (54), Expect = 9.7
Identities = 16/43 (37%), Positives = 20/43 (46%), Gaps = 4/43 (9%)
Frame = +1
Query: 202 EAESSGDQAGFIRHSACD----PRRRSRPFPHEHPSQGQRPQW 318
E ESSG I + D P RRSR H+H + G P +
Sbjct: 440 EHESSGSLNDSISDLSTDSHRQPSRRSRSRDHDHANVGSTPSY 482
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 9,500,881
Number of Sequences: 28952
Number of extensions: 221347
Number of successful extensions: 551
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 541
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 546
length of database: 12,070,560
effective HSP length: 73
effective length of database: 9,957,064
effective search space used: 527724392
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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