Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= an--0951
(501 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g52250.1 68418.m06485 transducin family protein / WD-40 repea... 31 0.58
At2g04020.1 68415.m00369 GDSL-motif lipase/hydrolase family prot... 30 0.76
At2g21470.2 68415.m02555 SUMO activating enzyme 2 (SAE2) nearly ... 29 1.3
At4g33985.1 68417.m04822 expressed protein 29 1.8
At2g21470.1 68415.m02554 SUMO activating enzyme 2 (SAE2) nearly ... 29 1.8
At5g27160.1 68418.m03241 hypothetical protein contains Pfam prof... 29 2.3
At4g25720.1 68417.m03702 glutamine cyclotransferase family prote... 29 2.3
At2g03980.1 68415.m00365 GDSL-motif lipase/hydrolase family prot... 29 2.3
At2g18010.1 68415.m02093 auxin-responsive family protein similar... 28 3.1
At5g53440.1 68418.m06641 expressed protein 27 5.4
At5g01400.1 68418.m00053 expressed protein contains low similari... 27 5.4
At3g56000.1 68416.m06222 glycosyl transferase family 2 protein s... 27 5.4
At1g22260.1 68414.m02782 expressed protein 27 5.4
At3g07840.1 68416.m00959 polygalacturonase, putative / pectinase... 27 7.1
At3g07830.1 68416.m00958 polygalacturonase, putative / pectinase... 27 7.1
At1g16140.1 68414.m01934 wall-associated kinase, putative contai... 27 7.1
At4g11640.1 68417.m01861 serine racemase, putative similar to se... 27 9.4
At4g01860.2 68417.m00244 transducin family protein / WD-40 repea... 27 9.4
At4g01860.1 68417.m00243 transducin family protein / WD-40 repea... 27 9.4
At3g43210.1 68416.m04561 kinesin motor family protein (NACK2) co... 27 9.4
At2g23120.1 68415.m02758 expressed protein 27 9.4
>At5g52250.1 68418.m06485 transducin family protein / WD-40 repeat
family protein contains 7 WD-40 repeats (PF00400);
similar to photomorphogenesis repressor PnCOP1
(GI:11127996) [Ipomoea nil]
Length = 385
Score = 30.7 bits (66), Expect = 0.58
Identities = 16/49 (32%), Positives = 23/49 (46%)
Frame = +1
Query: 202 TYRSSSCFEPRHETADSIGQYCCGSRRICSLPTRRSAASWVLQEGDNDG 348
+YR SS E R + + Y C ++ SL R + V+ GD DG
Sbjct: 97 SYRLSSLLESRDDHVTASESYICTPAKLSSLKWRPDFSGRVIGSGDYDG 145
>At2g04020.1 68415.m00369 GDSL-motif lipase/hydrolase family protein
similar to family II lipase EXL6 (GI:15054390), EXL1
(GI:15054382) [Arabidopsis thaliana]; contains Pfam
profile PF00657: Lipase/Acylhydrolase with GDSL-like
motif
Length = 261
Score = 30.3 bits (65), Expect = 0.76
Identities = 16/54 (29%), Positives = 24/54 (44%)
Frame = +2
Query: 320 GSYKKGTMMAGKNVADIVVIMKTLPTKEAVEGLSNKVNEEVNKLMNAEGSGAXV 481
G G GK +AD + I LP A GLS+ + ++ MN +G +
Sbjct: 77 GGKATGRFSNGKTIADYIAIYYKLPLVPAYLGLSDDRKDTISTGMNYASAGCGI 130
>At2g21470.2 68415.m02555 SUMO activating enzyme 2 (SAE2) nearly
identical to SUMO activating enzyme 2 [Arabidopsis
thaliana] GI:22652854; contains Pfam profiles PF00899:
ThiF family, PF02134: Repeat in ubiquitin-activating
(UBA) protein
Length = 700
Score = 29.5 bits (63), Expect = 1.3
Identities = 13/37 (35%), Positives = 20/37 (54%)
Frame = -3
Query: 115 FC**KVEGRTKHQDSGSVEGWTPHPAPAGDTASTTTD 5
FC + K + + GWTP PA G++AST+ +
Sbjct: 603 FCHREEFDEEKEPEGMVLSGWTPSPATNGESASTSNN 639
>At4g33985.1 68417.m04822 expressed protein
Length = 154
Score = 29.1 bits (62), Expect = 1.8
Identities = 14/40 (35%), Positives = 24/40 (60%), Gaps = 1/40 (2%)
Frame = +1
Query: 226 EPR-HETADSIGQYCCGSRRICSLPTRRSAASWVLQEGDN 342
+PR ET ++G YC +++ S +R S+ S + EG+N
Sbjct: 75 DPRLSETLPALGLYCAVNKQYSSRLSRTSSLSSIASEGEN 114
>At2g21470.1 68415.m02554 SUMO activating enzyme 2 (SAE2) nearly
identical to SUMO activating enzyme 2 [Arabidopsis
thaliana] GI:22652854; contains Pfam profiles PF00899:
ThiF family, PF02134: Repeat in ubiquitin-activating
(UBA) protein
Length = 625
Score = 29.1 bits (62), Expect = 1.8
Identities = 11/27 (40%), Positives = 17/27 (62%)
Frame = -3
Query: 85 KHQDSGSVEGWTPHPAPAGDTASTTTD 5
K + + GWTP PA G++AST+ +
Sbjct: 538 KEPEGMVLSGWTPSPATNGESASTSNN 564
>At5g27160.1 68418.m03241 hypothetical protein contains Pfam profile
PF03384: Drosophila protein of unknown function, DUF287
Length = 702
Score = 28.7 bits (61), Expect = 2.3
Identities = 18/68 (26%), Positives = 33/68 (48%)
Frame = +2
Query: 245 QTVLDNIVVAPGEFAACQLEEVRQVGSYKKGTMMAGKNVADIVVIMKTLPTKEAVEGLSN 424
+ V ++ PG ++C L+E G + M+A N + +V ++ E+V
Sbjct: 458 ENVQRHVPDVPGSSSSCSLKEAIDEGFKRVMAMLAKHN--ERLVTIERRQAGESVPPFEK 515
Query: 425 KVNEEVNK 448
KV+ +VNK
Sbjct: 516 KVHSDVNK 523
>At4g25720.1 68417.m03702 glutamine cyclotransferase family protein
contains Pfam profile: PF05096 glutamine
cyclotransferase
Length = 320
Score = 28.7 bits (61), Expect = 2.3
Identities = 15/38 (39%), Positives = 22/38 (57%)
Frame = +1
Query: 19 TRYPRPGRDGASTLQQTPSLDASSALRPFISRTRFS*M 132
TR P + S +Q P+ ++S+ R FISR RF+ M
Sbjct: 3 TRSPYKRQTKRSMIQSLPASSSASSRRRFISRKRFAMM 40
>At2g03980.1 68415.m00365 GDSL-motif lipase/hydrolase family protein
similar to Anther-specific proline-rich protein APG from
Brassica napus (SP|P40603 ), Arabidopsis thaliana
(GI:22599); contains Pfam profile PF00657:
Lipase/Acylhydrolase with GDSL-like motif
Length = 367
Score = 28.7 bits (61), Expect = 2.3
Identities = 15/54 (27%), Positives = 23/54 (42%)
Frame = +2
Query: 320 GSYKKGTMMAGKNVADIVVIMKTLPTKEAVEGLSNKVNEEVNKLMNAEGSGAXV 481
G G GK +AD + I LP A GLS + ++ +N +G +
Sbjct: 77 GGKATGRFSNGKTIADYIAIYYGLPLVPAYLGLSQEEKNSISTGINYASAGCGI 130
>At2g18010.1 68415.m02093 auxin-responsive family protein similar to
auxin-induced protein TGSAUR22 (GI:10185820) [Tulipa
gesnerian] ;similar to indole-3-acetic acid induced
protein ARG7 (SP:P32295) [Phaseolus aureus]
Length = 112
Score = 28.3 bits (60), Expect = 3.1
Identities = 14/52 (26%), Positives = 25/52 (48%)
Frame = -3
Query: 385 FHDDYDISNVLPGHHCPLLVGPNLPHFF*LAGCKFSWSHNNIVQYCLQFRDE 230
++ + D+ +P H P+ VGPN + SW H++ Q L+ +E
Sbjct: 38 YNQEDDLPQDVPKGHFPVYVGPNRSRYI----VPISWLHHSEFQTLLRLAEE 85
>At5g53440.1 68418.m06641 expressed protein
Length = 1181
Score = 27.5 bits (58), Expect = 5.4
Identities = 11/35 (31%), Positives = 18/35 (51%)
Frame = +1
Query: 163 SGPSKEAHGAMSVTYRSSSCFEPRHETADSIGQYC 267
+ P+KE H + T + +P ET D+ +YC
Sbjct: 820 TSPAKELHASSPKTMEEVAADDPVSETIDNTERYC 854
>At5g01400.1 68418.m00053 expressed protein contains low similarity to
symplekin SP:Q92797 from [Homo sapiens]
Length = 1467
Score = 27.5 bits (58), Expect = 5.4
Identities = 17/37 (45%), Positives = 20/37 (54%)
Frame = +1
Query: 46 GASTLQQTPSLDASSALRPFISRTRFS*MQTGSRRLR 156
G S + QTP + SSA P S TR S Q S+ LR
Sbjct: 1395 GQSDMSQTPQVSDSSAPEP-TSHTRTSDPQASSQTLR 1430
>At3g56000.1 68416.m06222 glycosyl transferase family 2 protein
similar to beta-(1-3)-glucosyl transferase GB:AAC62210
GI:3687658 from [Bradyrhizobium japonicum], cellulose
synthase from Agrobacterium tumeficiens [gi:710492] and
Agrobacterium radiobacter [gi:710493]; contains Pfam
glycosyl transferase, group 2 family protein domain
PF00535
Length = 535
Score = 27.5 bits (58), Expect = 5.4
Identities = 20/86 (23%), Positives = 36/86 (41%), Gaps = 6/86 (6%)
Frame = -3
Query: 448 LVHLLVNLIGESLDSLFRWQSFHDDYDISNVLPGHH------CPLLVGPNLPHFF*LAGC 287
+V +V L+G + + +W+SF D DI +H P+ + A C
Sbjct: 57 IVVAIVKLLGRTPQKVLKWESFKSD-DIELAPSSNHPMVLIQIPIFNEKEVCQLSIGAAC 115
Query: 286 KFSWSHNNIVQYCLQFRDEAQNSCLI 209
K SW + ++ L E ++ L+
Sbjct: 116 KLSWPLDRMIIQVLDDSTEEESQKLV 141
>At1g22260.1 68414.m02782 expressed protein
Length = 857
Score = 27.5 bits (58), Expect = 5.4
Identities = 11/31 (35%), Positives = 20/31 (64%)
Frame = +2
Query: 377 IMKTLPTKEAVEGLSNKVNEEVNKLMNAEGS 469
+ + TKEA+E N++NE++ +L N + S
Sbjct: 347 LFRVAATKEALESAGNELNEKIVELQNDKES 377
>At3g07840.1 68416.m00959 polygalacturonase, putative / pectinase,
putative strong similarity to polygalacturonase PGA3
[Arabidopsis thaliana] GI:3152948; contains
non-consensus AA acceptor splice site at exon 3
Length = 401
Score = 27.1 bits (57), Expect = 7.1
Identities = 12/26 (46%), Positives = 16/26 (61%)
Frame = +2
Query: 152 SVLTQALLKRHTELCPSPTDQAAVLS 229
S +TQALLK T C +PT V++
Sbjct: 34 SDITQALLKAFTSACQAPTASKVVIT 59
>At3g07830.1 68416.m00958 polygalacturonase, putative / pectinase,
putative strong similarity to polygalacturonase (PGA3)
GI:3152948 from [Arabidopsis thaliana]
Length = 397
Score = 27.1 bits (57), Expect = 7.1
Identities = 12/25 (48%), Positives = 16/25 (64%)
Frame = +2
Query: 152 SVLTQALLKRHTELCPSPTDQAAVL 226
S +TQALL+ T C SPT + V+
Sbjct: 34 SDITQALLRAFTTACQSPTPRKVVI 58
>At1g16140.1 68414.m01934 wall-associated kinase, putative contains
similarity to wall-associated kinase 4 GI:3355308 from
[Arabidopsis thaliana]
Length = 690
Score = 27.1 bits (57), Expect = 7.1
Identities = 17/52 (32%), Positives = 24/52 (46%), Gaps = 2/52 (3%)
Frame = +1
Query: 214 SSCFEPRHETADSIGQYCCGSRRICS--LPTRRSAASWVLQEGDNDGREERC 363
SSC + + ++ +G C + C LP R A V E +ND R E C
Sbjct: 145 SSCKDTK---SNEVGNSLCNGYKCCQARLPVERPQAVGVNIESNNDTRGEGC 193
>At4g11640.1 68417.m01861 serine racemase, putative similar to
serine racemase [Mus musculus] GI:6448865; contains Pfam
profile PF00291: Pyridoxal-phosphate dependent enzyme
Length = 346
Score = 26.6 bits (56), Expect = 9.4
Identities = 19/62 (30%), Positives = 32/62 (51%), Gaps = 3/62 (4%)
Frame = +2
Query: 200 SPTDQAAVLSLVTKLQTVLDNIVVAPGEFAACQLEEVRQVGS---YKKGTMMAGKNVADI 370
S + AA LSL K+Q + IVV G C+++ V + G + + TM + + +A
Sbjct: 98 SSGNHAAALSLAAKIQGIPAYIVVPKGA-PKCKVDNVIRYGGKVIWSEATMSSREEIASK 156
Query: 371 VV 376
V+
Sbjct: 157 VL 158
>At4g01860.2 68417.m00244 transducin family protein / WD-40 repeat
family protein contains ten G-protein beta-subunit
(beta-transducin) WD-40 repeats
Length = 1308
Score = 26.6 bits (56), Expect = 9.4
Identities = 12/45 (26%), Positives = 22/45 (48%)
Frame = +1
Query: 265 CCGSRRICSLPTRRSAASWVLQEGDNDGREERC*YRSHHENFANE 399
C R + S+ +R SW+L+ G + + E C + H ++E
Sbjct: 853 CESPRLLISVGAKRVVTSWLLRNGRHKKKGESCISDNGHNRASSE 897
>At4g01860.1 68417.m00243 transducin family protein / WD-40 repeat
family protein contains ten G-protein beta-subunit
(beta-transducin) WD-40 repeats
Length = 1308
Score = 26.6 bits (56), Expect = 9.4
Identities = 12/45 (26%), Positives = 22/45 (48%)
Frame = +1
Query: 265 CCGSRRICSLPTRRSAASWVLQEGDNDGREERC*YRSHHENFANE 399
C R + S+ +R SW+L+ G + + E C + H ++E
Sbjct: 853 CESPRLLISVGAKRVVTSWLLRNGRHKKKGESCISDNGHNRASSE 897
>At3g43210.1 68416.m04561 kinesin motor family protein (NACK2)
contains Pfam profile: PF00225 kinesin motor domain
Length = 938
Score = 26.6 bits (56), Expect = 9.4
Identities = 21/68 (30%), Positives = 36/68 (52%), Gaps = 6/68 (8%)
Frame = +2
Query: 278 GEFAACQLEEV-RQVGSYKKGTMMAGKNVADIVVIMK-----TLPTKEAVEGLSNKVNEE 439
GE A L+ + ++V S+K G A + VA ++ ++ L T+E V G + EE
Sbjct: 502 GEEANQALDLIHKEVTSHKLGDQQAAEKVAKMLSEIRDMQKSNLLTEEIVVGDKANLKEE 561
Query: 440 VNKLMNAE 463
+N+L + E
Sbjct: 562 INRLNSQE 569
>At2g23120.1 68415.m02758 expressed protein
Length = 83
Score = 26.6 bits (56), Expect = 9.4
Identities = 17/37 (45%), Positives = 20/37 (54%), Gaps = 3/37 (8%)
Frame = -3
Query: 103 KVEGRTKHQDS--GSVEGWTPHPAPAGDT-ASTTTDA 2
K G HQ+ G G T P P+GD A+TTTDA
Sbjct: 44 KAYGAEGHQEPTPGLGGGSTDAPTPSGDAPAATTTDA 80
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,005,417
Number of Sequences: 28952
Number of extensions: 237164
Number of successful extensions: 699
Number of sequences better than 10.0: 21
Number of HSP's better than 10.0 without gapping: 689
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 698
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 888318720
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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