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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= an--0927
         (472 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At2g01070.1 68415.m00013 expressed protein similar to membrane p...    34   0.042
At5g67330.1 68418.m08491 NRAMP metal ion transporter 4 (NRAMP4) ...    29   1.2  
At2g23150.1 68415.m02765 NRAMP metal ion transporter 3 (NRAMP3) ...    29   1.2  
At5g46450.1 68418.m05721 disease resistance protein (TIR-NBS-LRR...    28   3.7  
At1g62200.1 68414.m07016 proton-dependent oligopeptide transport...    28   3.7  
At4g18790.1 68417.m02775 NRAMP metal ion transporter 5, putative...    27   4.8  
At1g47240.1 68414.m05230 NRAMP metal ion transporter 2, putative...    27   4.8  
At4g37410.1 68417.m05296 cytochrome P450, putative similar to cy...    27   6.4  
At5g46470.1 68418.m05723 disease resistance protein (TIR-NBS-LRR...    27   8.5  
At5g23320.1 68418.m02728 isoprenylcysteine carboxyl methyltransf...    27   8.5  
At4g14160.2 68417.m02186 transport protein, putative similar to ...    27   8.5  
At1g08570.1 68414.m00950 thioredoxin family protein contains Pfa...    27   8.5  

>At2g01070.1 68415.m00013 expressed protein similar to membrane
           protein PTM1 precursor isolog GB:AAB65479
          Length = 496

 Score = 34.3 bits (75), Expect = 0.042
 Identities = 26/94 (27%), Positives = 42/94 (44%), Gaps = 6/94 (6%)
 Frame = +2

Query: 74  AFFEVVAIAWFYGVGRLSRNIKQMTGRRPS----IYFRFCWLIATPALLIALWVASLVDY 241
           AF +   I W +    LS+ ++Q+  +R S    IY +F   +A   +    W+   V +
Sbjct: 347 AFLDAFLILWIFT--SLSKTLEQLQMKRTSVKLEIYRKFSNALAVMVVASVAWIVYEVYF 404

Query: 242 --TPPSYRQYQYPAWAQAIGWIIASLSLLCIPVY 337
             T P   ++Q  AW     W + +  LLCI  Y
Sbjct: 405 KATDPFNERWQ-TAWTITAFWDVIAFLLLCIICY 437


>At5g67330.1 68418.m08491 NRAMP metal ion transporter 4 (NRAMP4)
           identical to metal transporter Nramp4 [Arabidopsis
           thaliana] gi|6468014|gb|AAF13279; member of the natural
           resistance-associated macrophage protein (NRAMP) metal
           transporter family, PMID:11500563
          Length = 512

 Score = 29.5 bits (63), Expect = 1.2
 Identities = 10/27 (37%), Positives = 18/27 (66%)
 Frame = +2

Query: 266 QYPAWAQAIGWIIASLSLLCIPVYAVI 346
           +YP WA+ + WI+A ++L+   +  VI
Sbjct: 122 EYPTWARMVLWIMAEIALIGADIQEVI 148


>At2g23150.1 68415.m02765 NRAMP metal ion transporter 3 (NRAMP3)
           identical to metal transporter Nramp3 [Arabidopsis
           thaliana] gi|6468012|gb|AAF13278; member of the natural
           resistance-associated macrophage protein (NRAMP) metal
           transporter family, PMID:11500563
          Length = 509

 Score = 29.5 bits (63), Expect = 1.2
 Identities = 10/27 (37%), Positives = 18/27 (66%)
 Frame = +2

Query: 266 QYPAWAQAIGWIIASLSLLCIPVYAVI 346
           +YP WA+ + W++A L+L+   +  VI
Sbjct: 126 EYPTWARMVLWVMAELALIGSDIQEVI 152


>At5g46450.1 68418.m05721 disease resistance protein (TIR-NBS-LRR
           class), putative domain signature TIR-NBS-LRR exists,
           suggestive of a disease resistance protein.
          Length = 1123

 Score = 27.9 bits (59), Expect = 3.7
 Identities = 13/32 (40%), Positives = 20/32 (62%), Gaps = 1/32 (3%)
 Frame = -2

Query: 363 GALNTTI-TAYTGMHNKEREAIIQPIACAHAG 271
           G +  T+  +Y G++NK+ EAI + IAC   G
Sbjct: 417 GKIEKTLRVSYDGLNNKKDEAIFRHIACLFNG 448


>At1g62200.1 68414.m07016 proton-dependent oligopeptide transport
           (POT) family protein contains Pfam profile: PF00854 POT
           family ; contains non-consensus GA donor site at intron
           4
          Length = 590

 Score = 27.9 bits (59), Expect = 3.7
 Identities = 11/19 (57%), Positives = 15/19 (78%)
 Frame = +2

Query: 296 WIIASLSLLCIPVYAVIVV 352
           W++ SL L+ IPVYA+I V
Sbjct: 564 WLLVSLGLVNIPVYALICV 582


>At4g18790.1 68417.m02775 NRAMP metal ion transporter 5, putative
           (NRAMP5) identical to heavy metal transporter
           [Arabidopsis thaliana]gi|12657269|emb|CAC27822; similar
           to metal transporter Nramp3 [Arabidopsis thaliana]
           gi|6468012|gb|AAF13278; member of the natural
           resistance-associated macrophage protein (NRAMP) metal
           transporter family, PMID:11500563
          Length = 530

 Score = 27.5 bits (58), Expect = 4.8
 Identities = 9/27 (33%), Positives = 18/27 (66%)
 Frame = +2

Query: 266 QYPAWAQAIGWIIASLSLLCIPVYAVI 346
           +YP+WA+ + W +A ++L+   +  VI
Sbjct: 140 EYPSWARILLWFMAEVALIGADIQEVI 166


>At1g47240.1 68414.m05230 NRAMP metal ion transporter 2, putative
           (NRAMP2) similar to metal transporter Nramp3
           [Arabidopsis thaliana] gi|6468012|gb|AAF13278; member of
           the natural resistance-associated macrophage protein
           (NRAMP) metal transporter family, PMID:11500563
          Length = 530

 Score = 27.5 bits (58), Expect = 4.8
 Identities = 10/27 (37%), Positives = 17/27 (62%)
 Frame = +2

Query: 266 QYPAWAQAIGWIIASLSLLCIPVYAVI 346
           +YP WA+ + W +A L+L+   +  VI
Sbjct: 138 EYPTWARYVLWSMAELALIGADIQEVI 164


>At4g37410.1 68417.m05296 cytochrome P450, putative similar to
           cytochrome p450 SP:O65790 from [Arabidopsis thaliana]
          Length = 501

 Score = 27.1 bits (57), Expect = 6.4
 Identities = 11/33 (33%), Positives = 17/33 (51%)
 Frame = +2

Query: 326 IPVYAVIVVFRAPGENLLEKLRYFHSPEFYLRL 424
           +P+    ++ + P   L  +L   H P FYLRL
Sbjct: 38  LPILGHHLLIKPPVHRLFHRLSNIHGPIFYLRL 70


>At5g46470.1 68418.m05723 disease resistance protein (TIR-NBS-LRR
           class), putative domain signature TIR-NBS-LRR exists,
           suggestive of a disease resistance protein.
          Length = 1127

 Score = 26.6 bits (56), Expect = 8.5
 Identities = 12/32 (37%), Positives = 20/32 (62%), Gaps = 1/32 (3%)
 Frame = -2

Query: 363 GALNTTI-TAYTGMHNKEREAIIQPIACAHAG 271
           G +  T+  +Y G++N++ EAI + IAC   G
Sbjct: 419 GKIGKTLRVSYDGLNNRKDEAIFRHIACIFNG 450


>At5g23320.1 68418.m02728 isoprenylcysteine carboxyl
           methyltransferase family protein / ICMT family protein
           similar to SP|O12947 Protein-S isoprenylcysteine
           O-methyltransferase (EC 2.1.1.100) (Isoprenylcysteine
           carboxylmethyltransferase) (Farnesyl cysteine carboxyl
           methyltransferase) (FCMT) {Xenopus laevis}; contains
           Pfam profile PF04140: Isoprenylcysteine carboxyl
           methyltransferase (ICMT) family
          Length = 197

 Score = 26.6 bits (56), Expect = 8.5
 Identities = 13/38 (34%), Positives = 23/38 (60%), Gaps = 3/38 (7%)
 Frame = +2

Query: 254 YRQYQYPAWAQAIGWIIASLSLLCIPVYAV---IVVFR 358
           YR  ++P++   + W + +  +LC PV AV   +VV+R
Sbjct: 124 YRLMRHPSYCGFLIWSVGTQVMLCNPVSAVAFAVVVWR 161


>At4g14160.2 68417.m02186 transport protein, putative similar to
           Swiss-Prot:Q15436 protein transport protein Sec23A [Homo
           sapiens]
          Length = 772

 Score = 26.6 bits (56), Expect = 8.5
 Identities = 16/66 (24%), Positives = 30/66 (45%), Gaps = 3/66 (4%)
 Frame = +2

Query: 221 VASLVDYTPPSYRQYQYPAWAQAIGWIIASLS---LLCIPVYAVIVVFRAPGENLLEKLR 391
           +++ +    PS   Y + +  QA    +AS++   +L +  Y  +VVF     +    + 
Sbjct: 622 ISNAIVMIQPSLTSYSFNSGPQAALLDVASIAADKILLLDAYFSVVVFHGMTISQWRNMG 681

Query: 392 YFHSPE 409
           Y H PE
Sbjct: 682 YHHQPE 687


>At1g08570.1 68414.m00950 thioredoxin family protein contains Pfam
           profile: PF00085 Thioredoxin; similar to ESTs gb|T46281,
           gb|R83933, gb|N65879, emb|F14466, gb|N96726,
           gb|AA042340, and emb|Z18150
          Length = 275

 Score = 26.6 bits (56), Expect = 8.5
 Identities = 16/48 (33%), Positives = 22/48 (45%)
 Frame = -3

Query: 275 LDIGTAGSWGEYNRRDLPPIVLLIEQV*QSANKIGNKLTDVDLSSV*C 132
           L IGTA  W E   +D    +   +++  S    G+KL  VD  S  C
Sbjct: 82  LRIGTAQKWWEKGLKDNMREISSAQELVDSLTNAGDKLVVVDFFSPGC 129


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,739,297
Number of Sequences: 28952
Number of extensions: 258566
Number of successful extensions: 686
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 675
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 686
length of database: 12,070,560
effective HSP length: 75
effective length of database: 9,899,160
effective search space used: 801831960
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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