BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= an--0895 (739 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g20560.1 68414.m02563 AMP-dependent synthetase and ligase fam... 33 0.26 At3g16910.1 68416.m02162 AMP-dependent synthetase and ligase fam... 31 1.1 At5g37150.1 68418.m04460 tRNA-splicing endonuclease positive eff... 29 2.4 At4g32700.1 68417.m04655 DNA-directed DNA polymerase family prot... 29 3.2 At5g51490.1 68418.m06386 pectinesterase family protein contains ... 29 4.3 At3g21060.1 68416.m02662 transducin family protein / WD-40 repea... 28 7.4 At2g17650.1 68415.m02042 AMP-dependent synthetase and ligase fam... 28 7.4 At1g54570.1 68414.m06223 esterase/lipase/thioesterase family pro... 27 9.8 >At1g20560.1 68414.m02563 AMP-dependent synthetase and ligase family protein similar to AMP-binding protein GI:1903034 from [Brassica napus]; contains Pfam AMP-binding domain PF00501; identical to adenosine monophosphate binding protein 1 AMPBP1 (AMPBP1) GI:20799710 Length = 556 Score = 32.7 bits (71), Expect = 0.26 Identities = 15/39 (38%), Positives = 21/39 (53%) Frame = -2 Query: 675 FTSFHWAILKEVYHPWTLCTWRTWWFMSLIETVSKLCSR 559 F+ FH L E Y P T+CTW+ W E +K+ +R Sbjct: 326 FSMFHSYGLTETYGPGTICTWKPEWDSLPREEQAKMKAR 364 >At3g16910.1 68416.m02162 AMP-dependent synthetase and ligase family protein similar to AMP-binding protein GI:1903034 from [Brassica napus]; contains Pfam AMP-binding domain PF00501; identical to cDNA adenosine monophosphate binding protein 7 AMPBP7 (AMPBP7) GI:20799722 Length = 569 Score = 30.7 bits (66), Expect = 1.1 Identities = 18/45 (40%), Positives = 22/45 (48%) Frame = -2 Query: 693 NQHGTQFTSFHWAILKEVYHPWTLCTWRTWWFMSLIETVSKLCSR 559 NQ G F H L E Y P T+C W+ W ET +KL +R Sbjct: 333 NQKG--FRVAHTYGLSETYGPSTVCAWKPEWDSLPPETQAKLNAR 375 >At5g37150.1 68418.m04460 tRNA-splicing endonuclease positive effector-related contains similarity to SEN1, a positive effector of tRNA-splicing endonuclease [Saccharomyces cerevisiae] gi|172574|gb|AAB63976 Length = 839 Score = 29.5 bits (63), Expect = 2.4 Identities = 15/72 (20%), Positives = 33/72 (45%) Frame = -1 Query: 403 GDSFIKLKNTLLNINFYYEDIEDADGKVHWKIFNHDVEYEVEKAVFRLENLLNDKNLGEQ 224 G F + + + +E+ +GK ++ + +E+ R E ++N +GE Sbjct: 341 GKLFSPFSGWMQRLESLIQFLENPEGKYERHVYELEEVERMEEEAERQEVVVNIPTIGEF 400 Query: 223 INKILNGLSQQI 188 + K N LS+++ Sbjct: 401 VKKNFNSLSEEV 412 >At4g32700.1 68417.m04655 DNA-directed DNA polymerase family protein similar to DNA helicase HEL308 [Homo sapiens] GI:19110782; contains Pfam profiles PF00271: Helicase conserved C-terminal domain, PF00176: SNF2 family N-terminal domain, PF00476: DNA polymerase family A Length = 1548 Score = 29.1 bits (62), Expect = 3.2 Identities = 10/22 (45%), Positives = 14/22 (63%), Gaps = 1/22 (4%) Frame = -3 Query: 191 NRRRSWTNNLR-ITHEGCCRKL 129 NR W N +R + H GCCR++ Sbjct: 1080 NRSGRWRNQIRRVAHNGCCRRV 1101 >At5g51490.1 68418.m06386 pectinesterase family protein contains Pfam profile: PF01095 pectinesterase Length = 536 Score = 28.7 bits (61), Expect = 4.3 Identities = 24/87 (27%), Positives = 36/87 (41%) Frame = -1 Query: 619 YVENMVVYVPNRNGFKVVFKDNYFTGLSSLTLQNLKFDMDKKIIAAQALVNLDVNNTYDL 440 Y + Y N NGF+ T LS + ++ MD+ I A L N N T Sbjct: 43 YPDPCKCYFKNHNGFQQP------TQLSEFRVMLVEAAMDRAISARAELTNSGKNCTDSK 96 Query: 439 SGRILVIPIKSSGDSFIKLKNTLLNIN 359 +L I GD+ ++L TL ++ Sbjct: 97 KQAVLADCIDLYGDTIMQLNRTLHGVS 123 >At3g21060.1 68416.m02662 transducin family protein / WD-40 repeat family protein contains 4 WD-40 repeats (PF00400); similar to Retinoblastoma-binding protein 5 (RBBP-5) [Homo sapiens](RBQ-3) Length = 547 Score = 27.9 bits (59), Expect = 7.4 Identities = 18/75 (24%), Positives = 35/75 (46%), Gaps = 2/75 (2%) Frame = -1 Query: 400 DSFIKLKNTLLNINFYYEDIEDADGKVHWK--IFNHDVEYEVEKAVFRLENLLNDKNLGE 227 D K+K + + +D+ K+HWK F+ D E+ V + + E+ + + Sbjct: 267 DGIEKMKTVGSKCLTLFREFQDSVTKMHWKAPCFSGDGEWVVGGSACKGEHKIYIWDRAG 326 Query: 226 QINKILNGLSQQIVD 182 + KIL G + ++D Sbjct: 327 HLVKILEGPKEALID 341 >At2g17650.1 68415.m02042 AMP-dependent synthetase and ligase family protein similar to AMP-binding protein GI:1903034 from [Brassica napus]; contains Pfam AMP-binding domain PF00501; identical to cDNA adenosine monophosphate binding protein 2 AMPBP2 (AMPBP2) GI:20799712 Length = 603 Score = 27.9 bits (59), Expect = 7.4 Identities = 14/39 (35%), Positives = 18/39 (46%) Frame = -2 Query: 675 FTSFHWAILKEVYHPWTLCTWRTWWFMSLIETVSKLCSR 559 F H L E Y P T C W+ W +E +KL +R Sbjct: 380 FNVSHLYGLTETYGPGTHCVWKPEWDSLSLEERTKLKAR 418 >At1g54570.1 68414.m06223 esterase/lipase/thioesterase family protein contains Interpro entry IPR000379 Length = 704 Score = 27.5 bits (58), Expect = 9.8 Identities = 16/47 (34%), Positives = 23/47 (48%) Frame = -1 Query: 724 KDPNLNDCALKSARNSVHQFSLGDPERGLPPLDPLYVENMVVYVPNR 584 K P LND + R++ QF L + G PLY+ ++ VP R Sbjct: 584 KIPILNDYITEVTRDT-KQFKLREESEGEVANQPLYLPGLIPKVPGR 629 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 14,453,540 Number of Sequences: 28952 Number of extensions: 291812 Number of successful extensions: 844 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 834 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 844 length of database: 12,070,560 effective HSP length: 79 effective length of database: 9,783,352 effective search space used: 1624036432 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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