Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= an--0894
(800 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g47810.3 68416.m05210 calcineurin-like phosphoesterase family... 272 2e-73
At3g47810.1 68416.m05209 calcineurin-like phosphoesterase family... 272 2e-73
At3g47810.2 68416.m05208 calcineurin-like phosphoesterase family... 202 2e-52
At3g52780.1 68416.m05815 purple acid phosphatase (PAP20) identic... 34 0.096
At1g20850.1 68414.m02612 cysteine endopeptidase, papain-type (XC... 30 2.1
At3g47910.1 68416.m05224 expressed protein low similarity to non... 29 2.7
At3g43960.1 68416.m04706 cysteine proteinase, putative contains ... 29 3.6
At3g27530.1 68416.m03441 vesicle tethering family protein contai... 29 3.6
At1g74060.1 68414.m08578 60S ribosomal protein L6 (RPL6B) simila... 29 3.6
At1g09510.1 68414.m01066 cinnamyl-alcohol dehydrogenase family /... 28 6.3
At1g06590.1 68414.m00698 expressed protein 28 6.3
At5g47510.1 68418.m05866 SEC14 cytosolic factor family protein /... 28 8.3
At4g36350.1 68417.m05161 calcineurin-like phosphoesterase family... 28 8.3
At4g00905.1 68417.m00123 expressed protein 28 8.3
At1g56360.1 68414.m06481 calcineurin-like phosphoesterase family... 28 8.3
>At3g47810.3 68416.m05210 calcineurin-like phosphoesterase family
protein contains Pfam profile: PF00149 calcineurin-like
phosphoesterase
Length = 190
Score = 272 bits (666), Expect = 2e-73
Identities = 115/181 (63%), Positives = 148/181 (81%)
Frame = +2
Query: 92 MLVLVLGDLHIPHRCSSLPAKFKKLLLPGRIQHILCTGNLCTKDSYDYLKTLASDVHVVR 271
+LVL LGDLH+PHR + LP KFK +L+PG+IQHI+CTGNLC K+ +DYLKT+ D+H+VR
Sbjct: 2 VLVLALGDLHVPHRAADLPPKFKSMLVPGKIQHIICTGNLCIKEIHDYLKTICPDLHIVR 61
Query: 272 GDFDENATYPEQKVVTVGQFRIGLIHGHQVVPWGDEESLALIQRQLDVDILISGHTHRFE 451
G+FDE+A YPE K +T+GQF++GL HGHQV+PWGD +SLA++QRQL VDIL++GHTH+F
Sbjct: 62 GEFDEDARYPENKTLTIGQFKLGLCHGHQVIPWGDLDSLAMLQRQLGVDILVTGHTHQFT 121
Query: 452 AYEHENKFYINPGSATGGYSPLYRDPTPSFVLMDIQSSTVVTYVYKLLGDEVKVERIEYK 631
AY+HE INPGSATG YS + +D PSFVLMDI V YVY+L+ EVKV++IE+K
Sbjct: 122 AYKHEGGVVINPGSATGAYSSINQDVNPSFVLMDIDGFRAVVYVYELIDGEVKVDKIEFK 181
Query: 632 K 634
K
Sbjct: 182 K 182
>At3g47810.1 68416.m05209 calcineurin-like phosphoesterase family
protein contains Pfam profile: PF00149 calcineurin-like
phosphoesterase
Length = 190
Score = 272 bits (666), Expect = 2e-73
Identities = 115/181 (63%), Positives = 148/181 (81%)
Frame = +2
Query: 92 MLVLVLGDLHIPHRCSSLPAKFKKLLLPGRIQHILCTGNLCTKDSYDYLKTLASDVHVVR 271
+LVL LGDLH+PHR + LP KFK +L+PG+IQHI+CTGNLC K+ +DYLKT+ D+H+VR
Sbjct: 2 VLVLALGDLHVPHRAADLPPKFKSMLVPGKIQHIICTGNLCIKEIHDYLKTICPDLHIVR 61
Query: 272 GDFDENATYPEQKVVTVGQFRIGLIHGHQVVPWGDEESLALIQRQLDVDILISGHTHRFE 451
G+FDE+A YPE K +T+GQF++GL HGHQV+PWGD +SLA++QRQL VDIL++GHTH+F
Sbjct: 62 GEFDEDARYPENKTLTIGQFKLGLCHGHQVIPWGDLDSLAMLQRQLGVDILVTGHTHQFT 121
Query: 452 AYEHENKFYINPGSATGGYSPLYRDPTPSFVLMDIQSSTVVTYVYKLLGDEVKVERIEYK 631
AY+HE INPGSATG YS + +D PSFVLMDI V YVY+L+ EVKV++IE+K
Sbjct: 122 AYKHEGGVVINPGSATGAYSSINQDVNPSFVLMDIDGFRAVVYVYELIDGEVKVDKIEFK 181
Query: 632 K 634
K
Sbjct: 182 K 182
>At3g47810.2 68416.m05208 calcineurin-like phosphoesterase family
protein contains Pfam profile: PF00149 calcineurin-like
phosphoesterase
Length = 180
Score = 202 bits (493), Expect = 2e-52
Identities = 86/140 (61%), Positives = 113/140 (80%)
Frame = +2
Query: 215 TKDSYDYLKTLASDVHVVRGDFDENATYPEQKVVTVGQFRIGLIHGHQVVPWGDEESLAL 394
+K+ +DYLKT+ D+H+VRG+FDE+A YPE K +T+GQF++GL HGHQV+PWGD +SLA+
Sbjct: 33 SKEIHDYLKTICPDLHIVRGEFDEDARYPENKTLTIGQFKLGLCHGHQVIPWGDLDSLAM 92
Query: 395 IQRQLDVDILISGHTHRFEAYEHENKFYINPGSATGGYSPLYRDPTPSFVLMDIQSSTVV 574
+QRQL VDIL++GHTH+F AY+HE INPGSATG YS + +D PSFVLMDI V
Sbjct: 93 LQRQLGVDILVTGHTHQFTAYKHEGGVVINPGSATGAYSSINQDVNPSFVLMDIDGFRAV 152
Query: 575 TYVYKLLGDEVKVERIEYKK 634
YVY+L+ EVKV++IE+KK
Sbjct: 153 VYVYELIDGEVKVDKIEFKK 172
>At3g52780.1 68416.m05815 purple acid phosphatase (PAP20) identical
to purple acid phosphatase GI:20257491 from [Arabidopsis
thaliana]
Length = 427
Score = 34.3 bits (75), Expect = 0.096
Identities = 21/67 (31%), Positives = 34/67 (50%), Gaps = 10/67 (14%)
Frame = +2
Query: 377 EESLALIQRQLDVDILISGHTHRFEAYE--HENKF------YINPGSATG--GYSPLYRD 526
+ES+ + + VD++ +GH H +E + +++KF YIN G G + YRD
Sbjct: 311 KESMETLLYKARVDLVFAGHVHAYERFSRVYQDKFDKCGPVYINIGDGGNLEGLATKYRD 370
Query: 527 PTPSFVL 547
P P L
Sbjct: 371 PNPEISL 377
>At1g20850.1 68414.m02612 cysteine endopeptidase, papain-type (XCP2)
identical to papain-type cysteine endopeptidase XCP2
GI:6708183 from [Arabidopsis thaliana]
Length = 356
Score = 29.9 bits (64), Expect = 2.1
Identities = 18/37 (48%), Positives = 22/37 (59%), Gaps = 2/37 (5%)
Frame = +2
Query: 344 IHGHQVVPWGDEESL--ALIQRQLDVDILISGHTHRF 448
I+GHQ VP DE+SL AL + L V I SG +F
Sbjct: 246 INGHQDVPTNDEKSLLKALAHQPLSVAIDASGREFQF 282
>At3g47910.1 68416.m05224 expressed protein low similarity to
nonmuscle myosin heavy chain (NMHC) [Homo sapiens]
GI:189036; contains Pfam profiles PF04780: Protein of
unknown function (DUF629), PF04781: Protein of unknown
function (DUF627)
Length = 1290
Score = 29.5 bits (63), Expect = 2.7
Identities = 24/86 (27%), Positives = 40/86 (46%), Gaps = 3/86 (3%)
Frame = +3
Query: 540 LC*WTFRVRQWSPTFTSSS---VMKSKLKELNTRRHDLLYVPPFSNMSEYYNRSVTLVYE 710
LC R R+ S FT S V++ + +ELN H+LL++ S + ++T V +
Sbjct: 748 LCLEEGRKRETSAEFTHESYETVLRKRREELNESDHELLFI-----SSRFELDAITNVLK 802
Query: 711 FIWLNTYTYF*YDAIPGCTEQILLEI 788
+ F Y+ GCT L ++
Sbjct: 803 DAETLNHNQFGYEESYGCTSSQLRDL 828
>At3g43960.1 68416.m04706 cysteine proteinase, putative contains
similarity to cysteine proteinase RD21A (thiol protease)
GI:435619, SP:P43297 from [Arabidopsis thaliana]
Length = 376
Score = 29.1 bits (62), Expect = 3.6
Identities = 14/41 (34%), Positives = 22/41 (53%)
Frame = +2
Query: 308 KVVTVGQFRIGLIHGHQVVPWGDEESLALIQRQLDVDILIS 430
K + + R+ I+GH+VVP DE SL + ++IS
Sbjct: 227 KAIEMKTTRVVTINGHEVVPVNDEMSLKKAVAYQPISVMIS 267
>At3g27530.1 68416.m03441 vesicle tethering family protein contains
Pfam PF04869: Uso1 / p115 like vesicle tethering
protein, head region and PF04871: Uso1 / p115 like
vesicle tethering protein, C terminal region
Length = 914
Score = 29.1 bits (62), Expect = 3.6
Identities = 16/38 (42%), Positives = 19/38 (50%), Gaps = 2/38 (5%)
Frame = +2
Query: 431 GHTHRFEAYEH-ENKFYINPGSATG-GYSPLYRDPTPS 538
G T F +E +N F +P GY PL R PTPS
Sbjct: 603 GLTSYFSKFEEMQNSFIFSPSKKPPQGYKPLTRTPTPS 640
>At1g74060.1 68414.m08578 60S ribosomal protein L6 (RPL6B) similar
to 60S ribosomal protein L6 (YL 16 like) GB:CAB57309
from [Cyanophora paradoxa]
Length = 233
Score = 29.1 bits (62), Expect = 3.6
Identities = 19/56 (33%), Positives = 30/56 (53%)
Frame = +2
Query: 122 IPHRCSSLPAKFKKLLLPGRIQHILCTGNLCTKDSYDYLKTLASDVHVVRGDFDEN 289
+P+R ++ PAK + + PG + I+ G K +LK LAS + +V G F N
Sbjct: 74 LPNRRTAKPAKLRASITPGTVL-IILAGRFKGK-RVVFLKQLASGLLLVTGPFKIN 127
>At1g09510.1 68414.m01066 cinnamyl-alcohol dehydrogenase family /
CAD family similar to cinnamyl alcohol dehydrogenase,
Eucalyptus gunnii [gi:1143445], CPRD14 protein, Vigna
unguiculata [gi:1854445]
Length = 322
Score = 28.3 bits (60), Expect = 6.3
Identities = 22/89 (24%), Positives = 39/89 (43%), Gaps = 2/89 (2%)
Frame = +2
Query: 401 RQLDVDILISGHTHRFEAYEHENKFYIN-PGSATGGYSPLYRDPTPSFVLMDI-QSSTVV 574
R +DV + H FE ++ I P + R+ P L + ++S ++
Sbjct: 225 RLVDVRDVALAHIKAFETPSANGRYIIEGPVVTINDIEKILREFFPDLNLGNKGEASEII 284
Query: 575 TYVYKLLGDEVKVERIEYKKT*SVIRSAI 661
+YKL ++VK IE+ T + +R I
Sbjct: 285 PVIYKLCVEKVKSLGIEFTPTEATLRDTI 313
>At1g06590.1 68414.m00698 expressed protein
Length = 916
Score = 28.3 bits (60), Expect = 6.3
Identities = 10/33 (30%), Positives = 19/33 (57%)
Frame = -2
Query: 661 NGGTYNRSCLLVFNSFNFDFITEELVNVGDHCR 563
N G + R C+L FN +F+ + ++ D+C+
Sbjct: 133 NLGMFVRRCILAFNLLSFEGVCHLFSSIEDYCK 165
>At5g47510.1 68418.m05866 SEC14 cytosolic factor family protein /
phosphoglyceride transfer family protein similar to
phosphatidylinositol transfer-like protein IV
(GI:14486707) [Lotus japonicus], SEC14 cytosolic factor
(Phosphatidylinositol/phosphatidylcholine transfer
protein) (PI/PCTP) (SP:P24859) [Kluyveromyces lactis]
and to SEC14 cytosolic factor (SP:P53989) [Candida
glabrata]
Length = 376
Score = 27.9 bits (59), Expect = 8.3
Identities = 13/56 (23%), Positives = 24/56 (42%)
Frame = +2
Query: 254 DVHVVRGDFDENATYPEQKVVTVGQFRIGLIHGHQVVPWGDEESLALIQRQLDVDI 421
D+H+ G ++ P + + + L+HGH GD +L + D D+
Sbjct: 7 DIHISDGTMNKEEQSPNNEEMVEAFRNLLLLHGHLPDKHGDHNTLRRFLKMRDFDL 62
>At4g36350.1 68417.m05161 calcineurin-like phosphoesterase family
protein contains Pfam profile: PF00149 calcineurin-like
phosphoesterase
Length = 466
Score = 27.9 bits (59), Expect = 8.3
Identities = 14/40 (35%), Positives = 21/40 (52%)
Frame = +2
Query: 413 VDILISGHTHRFEAYEHENKFYINPGSATGGYSPLYRDPT 532
VD+++SGH H +E E + N T G S +DP+
Sbjct: 344 VDLVLSGHVHSYERSERVSNIKYN---ITNGLSYPVKDPS 380
>At4g00905.1 68417.m00123 expressed protein
Length = 263
Score = 27.9 bits (59), Expect = 8.3
Identities = 16/54 (29%), Positives = 27/54 (50%)
Frame = -2
Query: 337 NAELSNCDDFLLWICRILIKISPNNMHITGQSLQIIIGVFCT*VSSTQYMLYPS 176
N +NC+DF L+ L+ + N + +GQ+ II +SS +L P+
Sbjct: 153 NVFQNNCEDFALYCKTGLLILDKNGVGRSGQASSIIGAPLAALLSSPLTLLIPN 206
>At1g56360.1 68414.m06481 calcineurin-like phosphoesterase family
protein contains Pfam profile: PF00149 calcineurin-like
phosphoesterase
Length = 466
Score = 27.9 bits (59), Expect = 8.3
Identities = 14/39 (35%), Positives = 20/39 (51%)
Frame = +2
Query: 413 VDILISGHTHRFEAYEHENKFYINPGSATGGYSPLYRDP 529
VD+++SGH H +E E + N T G S +DP
Sbjct: 344 VDLVLSGHVHAYERSERISNIKYN---ITNGLSSPVKDP 379
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 18,406,416
Number of Sequences: 28952
Number of extensions: 408376
Number of successful extensions: 1156
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 1113
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1155
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1814318400
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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