BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= an--0890 (808 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g05990.1 68414.m00627 calcium-binding protein, putative stron... 31 0.90 At2g27360.1 68415.m03296 lipase, putative similar to lipase [Ara... 28 6.3 At2g24140.1 68415.m02884 expressed protein contains Pfam PF04576... 28 8.4 >At1g05990.1 68414.m00627 calcium-binding protein, putative strong similarity to calcium-binding protein [Lotus japonicus] GI:18413495; contains INTERPRO:IPR002048 calcium-binding EF-hand domain Length = 150 Score = 31.1 bits (67), Expect = 0.90 Identities = 14/40 (35%), Positives = 22/40 (55%) Frame = -2 Query: 564 SVDASQEVMKNLSLNFGKALDECKKEMTLTDAINEDFYNF 445 +VD + V+ +L L GK LD+CKK + D + N+ Sbjct: 96 TVDELKAVLSSLGLKQGKTLDDCKKMIKKVDVDGDGRVNY 135 >At2g27360.1 68415.m03296 lipase, putative similar to lipase [Arabidopsis thaliana] GI:1145627; contains Pfam profile PF00657: Lipase/Acylhydrolase with GDSL-like motif Length = 394 Score = 28.3 bits (60), Expect = 6.3 Identities = 18/65 (27%), Positives = 35/65 (53%), Gaps = 3/65 (4%) Frame = +1 Query: 583 HERQSDLSLDRHFRFYLESLTC*AGSST--MKVLIVLSCVLVAVLADDKYT-DKYDKINL 753 H QS++SL + + ESL GSS+ + +I + +L+ + + Y +D+ N+ Sbjct: 131 HCSQSNISLGNQLKSFKESLPYLCGSSSPDCRDMIENAFILIGEIGGNDYNFPLFDRKNI 190 Query: 754 QEILE 768 +E+ E Sbjct: 191 EEVKE 195 >At2g24140.1 68415.m02884 expressed protein contains Pfam PF04576: Protein of unknown function, DUF593 Length = 273 Score = 27.9 bits (59), Expect = 8.4 Identities = 18/61 (29%), Positives = 32/61 (52%), Gaps = 3/61 (4%) Frame = -2 Query: 564 SVDASQEVMKNLSLNFG--KALDECKKEMT-LTDAINEDFYNFWKEGYEIKNRETGCAIM 394 S+D+ ++ KNL LN+ K +DE KE+T D++ + + +E + + G M Sbjct: 105 SLDSRRDHEKNLDLNWEKIKKVDEQLKELTDFRDSVRDQYKILKQETTSVSETKNGEKGM 164 Query: 393 C 391 C Sbjct: 165 C 165 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 17,427,606 Number of Sequences: 28952 Number of extensions: 358879 Number of successful extensions: 845 Number of sequences better than 10.0: 3 Number of HSP's better than 10.0 without gapping: 825 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 845 length of database: 12,070,560 effective HSP length: 80 effective length of database: 9,754,400 effective search space used: 1833827200 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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